##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921656_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3037888
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.98734515558177	33.0	31.0	34.0	28.0	34.0
2	31.122794520403648	33.0	31.0	34.0	28.0	34.0
3	31.198327588113848	34.0	31.0	34.0	28.0	34.0
4	34.67008691564666	37.0	35.0	37.0	32.0	37.0
5	34.37532555512251	37.0	35.0	37.0	31.0	37.0
6	34.34496762224282	37.0	35.0	37.0	31.0	37.0
7	34.2062416389281	37.0	35.0	37.0	30.0	37.0
8	34.14656333610719	37.0	35.0	37.0	30.0	37.0
9	35.70265296153117	39.0	37.0	39.0	30.0	39.0
10-11	35.573938538879645	39.0	37.0	39.0	30.0	39.0
12-13	35.482490467061325	39.0	37.0	39.0	30.0	39.0
14-15	36.69219240472328	40.0	37.0	41.0	30.0	41.0
16-17	36.64573117902964	40.0	37.0	41.0	30.0	41.0
18-19	36.59005615085218	40.0	37.0	41.0	30.0	41.0
20-21	36.52581645537953	40.0	37.0	41.0	29.0	41.0
22-23	36.43098856837382	40.0	37.0	41.0	29.0	41.0
24-25	36.34275374865696	40.0	37.0	41.0	28.5	41.0
26-27	36.20895618929993	40.0	37.0	41.0	28.0	41.0
28-29	36.08685655955716	40.0	36.5	41.0	27.0	41.0
30-31	35.930081359154784	40.0	36.0	41.0	27.0	41.0
32-33	35.884696703762614	40.0	36.0	41.0	27.0	41.0
34-35	36.0220859031011	40.0	37.0	41.0	27.5	41.0
36-37	36.0203080232056	40.0	36.5	41.0	27.0	41.0
38-39	35.90863043667179	40.0	36.0	41.0	27.0	41.0
40-41	35.82925670729138	40.0	36.0	41.0	27.0	41.0
42-43	35.699227555459586	40.0	36.0	41.0	26.0	41.0
44-45	35.570134580340024	40.0	35.5	41.0	26.0	41.0
46-47	35.41936667842923	40.0	35.0	41.0	25.0	41.0
48-49	35.27341923072872	40.0	35.0	41.0	24.5	41.0
50-51	35.111266939400004	39.0	35.0	41.0	24.0	41.0
52-53	34.938583482998716	39.0	35.0	41.0	24.0	41.0
54-55	34.71730162533971	39.0	35.0	41.0	23.0	41.0
56-57	34.49177767580635	39.0	34.5	40.5	23.0	41.0
58-59	34.24646020524786	38.0	34.0	40.0	22.0	41.0
60-61	33.961757971327444	38.0	34.0	40.0	22.0	41.0
62-63	33.657610484652494	37.0	34.0	40.0	20.5	41.0
64-65	33.3172809530832	37.0	34.0	40.0	20.0	41.0
66-67	32.96358621516001	36.0	33.0	39.0	18.5	41.0
68-69	32.60289434633535	36.0	33.0	39.0	17.5	40.5
70-71	32.21081636321023	35.0	33.0	38.5	13.0	40.0
72-73	31.854811961467966	35.0	32.0	37.0	11.0	39.5
74-75	31.460219896191038	35.0	32.0	37.0	7.5	39.0
76-77	28.8032613447237	32.0	28.0	34.5	4.0	36.5
78-79	30.348498364653338	34.5	31.0	35.5	2.0	37.0
80-81	30.532016815629806	35.0	31.0	35.5	2.0	37.0
82-83	30.360993558682875	35.0	31.0	35.0	2.0	37.0
84-85	30.161338732698507	35.0	31.0	35.0	2.0	36.0
86-87	29.951528989877175	35.0	31.0	35.0	2.0	36.0
88-89	29.68463353487686	34.0	31.0	35.0	2.0	36.0
90-91	29.481714105325807	34.0	31.0	35.0	2.0	35.0
92-93	29.314204638222343	34.0	31.0	35.0	2.0	35.0
94-95	29.201385469115387	34.0	31.0	35.0	2.0	35.0
96-97	28.98717464238313	34.0	30.5	35.0	2.0	35.0
98-99	28.763964635957613	34.0	30.0	35.0	2.0	35.0
100-101	27.643644038226554	33.0	27.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	83419.0
3	26165.0
4	13669.0
5	9214.0
6	7754.0
7	7641.0
8	7762.0
9	8479.0
10	9029.0
11	9642.0
12	10299.0
13	10801.0
14	11381.0
15	12167.0
16	12858.0
17	13341.0
18	12533.0
19	11875.0
20	11433.0
21	11334.0
22	12283.0
23	13778.0
24	16043.0
25	19278.0
26	23219.0
27	27423.0
28	33109.0
29	40716.0
30	49223.0
31	60885.0
32	77444.0
33	101783.0
34	143361.0
35	217136.0
36	386048.0
37	728301.0
38	707717.0
39	79345.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.52784984640866	19.07793104312228	12.08189296802834	27.312326142440728
2	15.49091407045167	20.615369118768196	36.11062831892199	27.783088491858148
3	18.016629428168766	23.711107387444212	28.739934165715542	29.532329018671483
4	12.831445457229886	15.865631986126424	34.45416409757723	36.84875845906646
5	13.850444697818507	37.18340817672413	34.73766071793683	14.228486407520535
6	35.32924138003681	35.35649766017344	15.672821933536813	13.641439026252932
7	29.80952556512946	31.20075526155013	20.31684512398087	18.67287404933954
8	27.410227105146735	35.16647091663682	18.838910453578276	18.584391524638168
9	26.463986316805073	14.602880225630512	18.32309760040449	40.61003585715993
10-11	26.077989491800718	24.955837109079376	27.635991076527276	21.330182322592627
12-13	26.222523163380217	22.16737214837862	27.501903467454408	24.108201220786754
14-15	23.105300659749364	23.957561276137607	24.248550199004555	28.688587865108474
16-17	23.878369426122593	27.259024568882158	25.11172648756743	23.750879517427816
18-19	23.862541464398966	26.550711737622073	26.299530555686314	23.28721624229265
20-21	24.17663771335198	25.765641093670943	26.3558884340028	23.70183275897427
22-23	24.02139249373249	25.660195504245053	26.093605162533972	24.224806839488487
24-25	23.852963521833704	25.88856607520001	26.192693438038205	24.06577696492808
26-27	23.747727184071795	26.072085236502062	26.095210475058238	24.084977104367905
28-29	23.91267492223575	26.154003661314274	25.984797085433193	23.94852433101678
30-31	23.9644705957108	25.987535604386885	25.910363615382575	24.13763018451974
32-33	23.738767582159817	26.07965366540193	25.851787228088657	24.329791524349595
34-35	24.062074138623224	26.04036715248436	25.994755387776543	23.902803321115876
36-37	24.171075728884027	25.796359344948733	26.031547735145182	24.001017191022058
38-39	23.97468635688451	25.984849669383742	26.00050199357284	24.03996198015891
40-41	24.07724982104533	25.83759754860498	26.083879332295407	24.001273298054286
42-43	23.945785809802285	25.860707304210074	25.9741679170982	24.21933896888944
44-45	23.94820770642782	25.865695835495078	26.079050009349263	24.107046448727843
46-47	24.08573528796956	25.85394083618603	25.949175200615976	24.111148675228435
48-49	23.826514840513735	26.05092117222923	25.918382194873118	24.204181792383913
50-51	23.88406476464296	26.121111679687882	25.921197235038058	24.0736263206311
52-53	24.203048495865133	25.889237895784685	25.86573424547686	24.041979362873317
54-55	23.655465857125858	25.885785023659615	26.183486809327167	24.275262309887363
56-57	23.734161119770008	26.013761581902116	26.03858332377279	24.21349397455508
58-59	23.710380872034403	25.887100557624642	26.086808370542602	24.31571019979835
60-61	23.609615537186926	26.0264703142247	26.15079269715871	24.213121451429668
62-63	23.729365189637107	25.906288707246294	26.118637360061264	24.24570874305533
64-65	23.48353099129532	25.826381303301048	26.103677646664426	24.586410058739204
66-67	23.504287439255513	25.915753811331594	26.034937178820833	24.54502157059206
68-69	23.723408477176662	25.742476270646936	26.03241067107455	24.501704581101848
70-71	23.564191901682463	25.686131933847793	26.046900772511407	24.702775391958337
72-73	23.565869214351938	25.902676100188334	25.895168445591153	24.636286239868575
74-75	23.472855623298212	25.982860758189112	25.867667325581223	24.67661629293145
76-77	23.57300923638048	25.77206811393493	25.94778631031045	24.707136339374138
78-79	23.51184951145978	25.82039292414346	25.933079123633778	24.734678440762988
80-81	23.57506019990932	25.91101925557157	25.7939050895995	24.72001545491961
82-83	23.516911307971462	25.929190529476564	25.841655374242013	24.712242788309965
84-85	23.43138917751983	25.877224905437917	25.82339409129008	24.867991825752174
86-87	23.425537206280296	26.000932195154892	25.7370023831915	24.836528215373317
88-89	23.30849753897855	25.86913175955606	25.719935429135454	25.10243527232993
90-91	23.25336288709042	25.880600114259998	25.770301742473272	25.095735256176305
92-93	23.325903976037235	25.741512707165036	25.896878764251152	25.035704552546576
94-95	23.19896115627823	25.54493535703093	25.999832763147847	25.256270723543
96-97	23.182972183771703	25.417343837527063	26.037814241436124	25.361869737265113
98-99	23.086598403745793	25.518506107255696	25.884717607317253	25.510177881681262
100-101	23.05611277857402	25.457670837774476	25.880333732514956	25.605882651136547
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	29.0
1	23.0
2	39.0
3	59.0
4	50.0
5	48.5
6	110.0
7	238.0
8	347.5
9	355.0
10	289.0
11	251.0
12	263.5
13	283.5
14	318.5
15	346.5
16	388.0
17	473.0
18	506.5
19	550.5
20	647.5
21	791.5
22	1006.0
23	1247.0
24	1515.5
25	1959.0
26	2746.0
27	3966.5
28	5830.0
29	8521.5
30	12214.5
31	17323.0
32	25443.5
33	31206.0
34	34903.0
35	41060.5
36	48998.0
37	57315.0
38	65241.0
39	73271.0
40	80805.0
41	88222.5
42	95316.0
43	103127.5
44	110349.5
45	118144.0
46	124050.5
47	128215.5
48	132311.0
49	133929.5
50	134270.5
51	134664.5
52	134648.5
53	133863.0
54	131097.0
55	123911.5
56	113884.0
57	102748.5
58	92821.0
59	81411.0
60	67613.0
61	56076.5
62	46048.5
63	34816.0
64	24713.5
65	18049.0
66	13425.5
67	10246.5
68	7803.0
69	5847.5
70	4490.5
71	3297.5
72	2271.5
73	1639.0
74	1223.5
75	891.5
76	605.5
77	354.5
78	195.5
79	109.5
80	70.0
81	50.0
82	31.5
83	18.5
84	11.5
85	11.5
86	9.5
87	4.5
88	4.0
89	3.0
90	1.5
91	2.0
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14859007310341923
2	1.3167042366275518E-4
3	0.008591495143994776
4	0.0056289106115827835
5	0.021626867086607538
6	0.0018433859312785724
7	0.0
8	0.0
9	3.2917605915688795E-5
10-11	0.04615048349379569
12-13	3.620936650725768E-4
14-15	0.004723676448901342
16-17	1.6458802957844397E-5
18-19	0.0013496218425432405
20-21	2.962584532411991E-4
22-23	0.0
24-25	5.266816946510207E-4
26-27	0.0026827848821286368
28-29	0.005958086670739672
30-31	0.01700194345545326
32-33	0.011949090947395033
34-35	0.00969423494217035
36-37	0.0036373954536836122
38-39	1.6458802957844397E-5
40-41	0.0035386426359365454
42-43	0.008887753597235974
44-45	0.0071102028777887795
46-47	0.003917195103966967
48-49	0.009579023321465438
50-51	0.0029132081235384586
52-53	0.002040891566772705
54-55	0.0038349010891777446
56-57	0.007521672951734889
58-59	0.014829381465017801
60-61	0.0072747909073672245
62-63	0.021923125539848737
64-65	0.040982419365032545
66-67	0.05958086670739672
68-69	0.04975496134156361
70-71	0.06618084669349232
72-73	0.03250613584174268
74-75	0.027074730865654033
76-77	0.2177170455263657
78-79	0.08122419259696209
80-81	0.06456788400362357
82-83	0.027074730865654033
84-85	0.050133513809594035
86-87	0.06725066888575221
88-89	0.2850006320180336
90-91	0.2907118366444056
92-93	0.04972204373564792
94-95	0.009216929656392862
96-97	0.014302699770366783
98-99	8.558577538079086E-4
100-101	1.975056354941328E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3037888.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.59827848812898
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.37419993875415	46.709673942341716
2	11.94628777195245	14.239563710643042
3	3.9804528922262867	7.116844199393421
4	1.8061425289971147	4.305719417296945
5	1.0168044025915874	3.0299895976804527
6	0.6165737962638769	2.2048042090930458
7	0.41293589519509516	1.7227187935707484
8	0.3174767601283894	1.513685469091254
9	0.23331533822434106	1.2514673252741657
>10	1.2175935227731487	12.760645477087266
>50	0.06002523121009951	2.420409744168183
>100	0.016419319718570583	1.6375991743018106
>500	9.970886057436843E-4	0.427759262291241
>1k	7.755133591634909E-4	0.659119677766806
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.2917605915688795E-5
2	0.0	0.0	0.0	3.2917605915688795E-5	3.2917605915688795E-5
3	0.0	0.0	0.0	6.583521183137759E-5	3.2917605915688795E-5
4	0.0	0.0	0.0	6.583521183137759E-5	3.2917605915688795E-5
5	0.0	0.0	0.0	6.583521183137759E-5	6.583521183137759E-5
6	0.0	0.0	0.0	6.583521183137759E-5	1.6458802957844396E-4
7	0.0	0.0	0.0	6.583521183137759E-5	1.6458802957844396E-4
8	0.0	0.0	0.0	6.583521183137759E-5	1.9750563549413277E-4
9	0.0	0.0	0.0	6.583521183137759E-5	2.3042324140982155E-4
10-11	0.0	0.0	0.0	9.875281774706638E-5	2.3042324140982155E-4
12-13	0.0	0.0	0.0	9.875281774706638E-5	3.4563486211473235E-4
14-15	0.0	0.0	0.0	9.875281774706638E-5	4.7730528577748753E-4
16-17	0.0	0.0	0.0	9.875281774706638E-5	5.266816946510207E-4
18-19	0.0	0.0	0.0	9.875281774706638E-5	5.266816946510207E-4
20-21	0.0	0.0	0.0	1.4812922662059956E-4	6.58352118313776E-4
22-23	0.0	0.0	0.0	1.6458802957844396E-4	7.571049360608423E-4
24-25	1.6458802957844397E-5	0.0	0.0	2.797996502833548E-4	8.558577538079087E-4
26-27	3.2917605915688795E-5	0.0	0.0	6.58352118313776E-4	8.558577538079087E-4
28-29	3.2917605915688795E-5	0.0	0.0	0.004295747571997388	8.558577538079087E-4
30-31	3.2917605915688795E-5	1.6458802957844397E-5	0.0	0.017578001558977815	8.72316556765753E-4
32-33	1.1521162070491077E-4	3.2917605915688795E-5	0.0	0.04481732045421029	9.216929656392862E-4
34-35	1.3167042366275518E-4	3.2917605915688795E-5	0.0	0.07801472602018245	9.87528177470664E-4
36-37	1.3167042366275518E-4	3.2917605915688795E-5	0.0	0.13402403248572692	9.87528177470664E-4
38-39	1.3167042366275518E-4	6.583521183137759E-5	0.0	0.22853047906966945	0.0010039869804285082
40-41	1.3167042366275518E-4	1.1521162070491077E-4	0.0	0.3297356584574547	0.0010204457833863526
42-43	1.3167042366275518E-4	1.6458802957844396E-4	0.0	0.4371787241662629	0.001036904586344197
44-45	1.3167042366275518E-4	1.6458802957844396E-4	0.0	0.564948411528009	0.0010533633893020414
46-47	1.3167042366275518E-4	1.6458802957844396E-4	0.0	0.7178671498093412	0.0010698221922598858
48-49	1.3167042366275518E-4	1.8104683253628836E-4	0.0	0.8772706564560642	0.0010862809952177303
50-51	1.3167042366275518E-4	1.9750563549413277E-4	0.0	1.052046685065414	0.0010862809952177303
52-53	1.3167042366275518E-4	1.9750563549413277E-4	0.0	1.224337434428129	0.0012673278277540185
54-55	1.3167042366275518E-4	2.3042324140982155E-4	0.0	1.421711399498599	0.001398998251416774
56-57	1.3167042366275518E-4	2.3042324140982155E-4	0.0	1.6465880243116269	0.0014154570543746183
58-59	1.3167042366275518E-4	2.3042324140982155E-4	0.0	1.8833972812690922	0.001448374660290307
60-61	1.3167042366275518E-4	2.3042324140982155E-4	0.0	2.1403850306528747	0.0014812922662059958
62-63	1.3167042366275518E-4	2.3042324140982155E-4	0.0	2.4031333610719026	0.0015142098721216846
64-65	1.3167042366275518E-4	2.3042324140982155E-4	0.0	2.684480138833295	0.0015142098721216846
66-67	1.3167042366275518E-4	2.6334084732551036E-4	0.0	3.0071549708218344	0.0015471274780373733
68-69	1.3167042366275518E-4	2.6334084732551036E-4	0.0	3.3643274538100156	0.0015800450839530621
70-71	1.3167042366275518E-4	2.6334084732551036E-4	0.0	3.7380739513767463	0.001612962689868751
72-73	1.3167042366275518E-4	2.6334084732551036E-4	0.0	4.130122637832599	0.0018104683253628837
74-75	1.3167042366275518E-4	2.6334084732551036E-4	0.0	4.525364332062275	0.0018433859312785724
76-77	1.3167042366275518E-4	2.6334084732551036E-4	0.0	4.959860271346409	0.0018433859312785724
78-79	1.3167042366275518E-4	2.6334084732551036E-4	0.0	5.448489213558894	0.0018763035371942613
80-81	1.3167042366275518E-4	2.6334084732551036E-4	0.0	5.964736027134641	0.0018763035371942613
82-83	1.3167042366275518E-4	2.6334084732551036E-4	0.0	6.49493990561864	0.0020079739608570165
84-85	1.3167042366275518E-4	2.6334084732551036E-4	0.0	7.038031026818632	0.0020079739608570165
86-87	1.3167042366275518E-4	2.6334084732551036E-4	0.0	7.623207307181831	0.0020079739608570165
88-89	1.3167042366275518E-4	2.6334084732551036E-4	0.0	8.258566477763498	0.0020079739608570165
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1790	0.0	40.359653	1
GTATCAA	2865	0.0	34.671974	1
GTACATG	6450	0.0	28.664658	1
TCAACGC	3365	0.0	28.349031	4
CAACGCA	3415	0.0	27.813765	5
ATCAACG	3560	0.0	26.796646	3
TACATGG	7055	0.0	26.236263	2
ACATGGG	6960	0.0	25.844275	3
AACGCAG	3725	0.0	25.75193	6
TATCAAC	3810	0.0	25.038336	2
CATGGGG	5635	0.0	23.666803	4
ACGCAGA	4670	0.0	19.930761	7
GTATAGG	670	0.0	19.1534	1
CGCAGAG	4960	0.0	18.573969	8
TATACTG	1310	0.0	18.489252	5
GAGTACA	4685	0.0	17.44921	1
GTATAGC	880	0.0	16.7431	1
AGAGTAC	4835	0.0	16.156754	10-11
TTATACT	1225	0.0	15.884554	4
ATGGGGG	3450	0.0	15.417693	5
>>END_MODULE