FastQCFastQC Report
Mon 15 Aug 2016
SRR2921655_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921655_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2726282
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19300.045.746741
GTATCAA33950.041.945561
TCAACGC42000.033.1185044
CAACGCA42900.032.4225165
ATCAACG43800.031.6467553
AACGCAG45750.030.5934336
TATCAAC46400.030.2826732
GTACATG75650.028.236321
TACATGG79600.025.7626862
ACGCAGA54800.025.0247867
ACATGGG81750.024.0399193
CGCAGAG58050.023.4624088
CATGGGG66850.021.2335264
TAAGGCG6700.019.8389935
GCAGAGT69300.019.1067929
AGAGTAC55900.018.29617310-11
TATACCG2352.544355E-618.1807175
GAGTACA55200.017.8006481
CAGAGTA60200.017.0681410-11
AGTACAT56350.016.1743852
Produced by FastQC (version 0.11.5)