##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921655_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2726282
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.697838668193533	31.0	31.0	34.0	27.0	34.0
2	30.76705711294723	33.0	31.0	34.0	27.0	34.0
3	30.82611006491625	33.0	31.0	34.0	27.0	34.0
4	34.28204602458587	37.0	35.0	37.0	31.0	37.0
5	34.279664025951824	37.0	35.0	37.0	30.0	37.0
6	34.321515896007824	37.0	35.0	37.0	30.0	37.0
7	34.238989583616075	37.0	35.0	37.0	30.0	37.0
8	34.207785548230156	37.0	35.0	37.0	30.0	37.0
9	35.804583311631006	39.0	37.0	39.0	30.0	39.0
10-11	35.713030236784014	39.0	37.0	39.0	30.0	39.0
12-13	35.61069104369981	39.0	37.0	39.0	30.0	39.0
14-15	36.80189045007083	40.0	37.0	41.0	30.0	41.0
16-17	36.73821710299961	40.0	37.0	41.0	30.0	41.0
18-19	36.69668269826819	40.0	37.0	41.0	30.0	41.0
20-21	36.619449675418764	40.0	37.0	41.0	30.0	41.0
22-23	36.52109796418712	40.0	37.0	41.0	29.5	41.0
24-25	36.410081568964614	40.0	37.0	41.0	29.0	41.0
26-27	36.26962562933695	40.0	37.0	41.0	28.5	41.0
28-29	36.13674924310838	40.0	36.0	41.0	28.0	41.0
30-31	35.99424179156815	40.0	36.0	41.0	27.0	41.0
32-33	35.823881572045735	40.0	36.0	41.0	27.0	41.0
34-35	35.65826664299585	40.0	36.0	41.0	26.5	41.0
36-37	35.63043680000821	39.5	35.5	41.0	26.5	41.0
38-39	35.822237391436396	40.0	36.0	41.0	27.0	41.0
40-41	35.88580528353266	40.0	36.0	41.0	27.0	41.0
42-43	35.73051320443006	40.0	36.0	41.0	26.0	41.0
44-45	35.60958129056348	40.0	35.5	41.0	26.0	41.0
46-47	35.454137539696916	40.0	35.0	41.0	25.5	41.0
48-49	35.28923640327743	40.0	35.0	41.0	24.0	41.0
50-51	33.57771096313587	38.0	33.0	39.5	22.0	40.0
52-53	33.701865214236825	37.5	33.5	39.5	22.5	40.0
54-55	34.61252632706375	39.0	35.0	40.5	23.0	41.0
56-57	34.61562688672706	39.0	35.0	41.0	23.0	41.0
58-59	34.413201018823436	38.5	34.5	41.0	22.5	41.0
60-61	34.14977577521328	38.0	34.0	40.5	22.0	41.0
62-63	33.84730486428036	37.0	34.0	40.0	22.0	41.0
64-65	33.541831879460744	37.0	34.0	40.0	20.5	41.0
66-67	33.186622293658544	36.0	34.0	39.0	20.0	41.0
68-69	32.81563921120412	36.0	33.0	39.0	18.5	41.0
70-71	32.39814149820158	35.0	33.0	38.5	17.0	40.5
72-73	32.02749183686794	35.0	33.0	37.5	13.0	40.0
74-75	31.62296783678284	35.0	32.5	37.0	9.0	39.0
76-77	31.251839684962892	35.0	32.0	36.5	7.0	39.0
78-79	30.91367840891001	35.0	32.0	36.0	2.0	38.0
80-81	30.605625903703285	35.0	32.0	35.5	2.0	37.0
82-83	30.304221280117027	35.0	31.5	35.0	2.0	37.0
84-85	30.050495693402226	35.0	31.0	35.0	2.0	36.0
86-87	29.822703960925537	35.0	31.0	35.0	2.0	36.0
88-89	29.613481474036803	35.0	31.0	35.0	2.0	36.0
90-91	29.395291462878745	35.0	31.0	35.0	2.0	35.0
92-93	29.203229159712752	35.0	31.0	35.0	2.0	35.0
94-95	28.975174431698555	34.5	31.0	35.0	2.0	35.0
96-97	28.737381716198104	34.0	30.0	35.0	2.0	35.0
98-99	28.466731431304613	34.0	30.0	35.0	2.0	35.0
100-101	27.38956700003888	33.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	80801.0
3	14520.0
4	10217.0
5	7712.0
6	7036.0
7	7291.0
8	7595.0
9	8098.0
10	8623.0
11	9385.0
12	9407.0
13	9449.0
14	9744.0
15	10641.0
16	10553.0
17	10938.0
18	10720.0
19	10281.0
20	10525.0
21	11163.0
22	12031.0
23	14019.0
24	16378.0
25	19745.0
26	23353.0
27	27611.0
28	32414.0
29	38177.0
30	45919.0
31	56331.0
32	70197.0
33	91718.0
34	129919.0
35	193236.0
36	334766.0
37	620763.0
38	635428.0
39	99573.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.33872875982009	17.52039615063941	12.650053724297475	25.490821365243022
2	16.482082203767444	19.289351653038466	38.53957683213568	25.688989311058403
3	19.44297430755329	22.956169204151486	28.688650942849154	28.912205545446067
4	13.732786074782785	15.281374111753934	35.364351094523215	35.621488718940064
5	15.066335329503943	36.23312072409722	33.79231678005459	14.908227166344249
6	34.651897399176306	34.06987693785191	16.77698007462158	14.501245588350201
7	29.901414475204678	30.79240399155086	20.620897313898453	18.685284219346006
8	27.03338355596008	33.82092203459968	19.843440407680063	19.302254001760176
9	27.033318538136758	15.122353830182936	18.91126917108994	38.93305846059037
10-11	26.133383420196623	24.515972708039932	27.596975125195762	21.753668746567687
12-13	26.582935608360536	22.057033926193938	27.267509284733414	24.092521180712115
14-15	23.86904262827681	23.629414863159354	24.31270533527247	28.188837173291365
16-17	24.25727399022118	26.5474616545552	25.163703418477763	24.031560936745862
18-19	24.271739594963243	26.043493404896072	26.239825062330347	23.444941937810334
20-21	24.78587105762537	25.31346848255665	26.010285233160385	23.890375226657593
22-23	24.707679211255822	25.050461501204435	25.832013928511806	24.40984535902794
24-25	24.530527628971893	25.261337785279224	25.765887576135615	24.44224700961327
26-27	24.280420805473774	25.437232295994644	25.838648432985718	24.443698465545864
28-29	24.40659841541917	25.544565533395147	25.690697672285793	24.35813837889989
30-31	24.656931968942246	25.354834556055337	25.498197236306492	24.49003623869593
32-33	24.417219638690298	25.454370041089103	25.457671549107914	24.670738771112685
34-35	24.73794729985439	25.41913484046841	25.531822407768693	24.311095451908507
36-37	24.75658507321659	25.282782037886697	25.507852239358638	24.452780649538074
38-39	24.60240836574762	25.353922214510938	25.585126206640542	24.4585432131009
40-41	24.747427507485565	25.248005774971965	25.638891120283674	24.365675597258797
42-43	24.606746859175736	25.2336069023337	25.468546180895157	24.69110005759541
44-45	24.477975713887297	25.284211895543336	25.5744040084175	24.66340838215187
46-47	24.655314332750283	25.288260844002174	25.4642416180383	24.592183205209235
48-49	24.432785465872314	25.301414689835255	25.500725526725788	24.76507431756664
50-51	24.400542743832812	25.394054254172783	25.609865664470767	24.595537337523645
52-53	24.61321022012134	25.26748496333026	25.55989476627979	24.559410050268614
54-55	24.34198849286013	25.1148821486462	25.761235483324498	24.781893875169178
56-57	24.288661263759835	25.220698521731027	25.74100407193251	24.74963614257662
58-59	24.41969930595638	25.09103017212627	25.75170295102223	24.737567570895116
60-61	24.350984783080897	25.103377506980344	25.839029157400272	24.706608552538487
62-63	24.399679630697023	25.160915018532947	25.826642283125544	24.612763067644483
64-65	24.26873818774734	24.949131290696343	25.781735478301837	25.000395043254482
66-67	24.21295142846854	25.095726096219977	25.742235529558233	24.94908694575325
68-69	24.312683429257625	25.051746939186458	25.74605435330197	24.889515278253942
70-71	24.21067734804417	24.802512031889812	25.856240480169635	25.13057013989638
72-73	24.245989232105398	24.925785383289533	25.76206748867879	25.066157895926278
74-75	24.22125061067666	25.063070336171183	25.734350738865935	24.981328314286223
76-77	24.22354326002792	24.79368557238463	25.851736542919962	25.131034624667485
78-79	24.157473601424464	24.80803666997118	25.89260858260345	25.141881146000912
80-81	24.299095305102288	24.934305834252044	25.709716598520664	25.056882262125008
82-83	24.188825742783504	24.904948520865013	25.819306168767024	25.086919567584452
84-85	24.20036884284928	24.894264559847286	25.81646356298664	25.08890303431679
86-87	24.149840219125956	24.966673644692992	25.808723537642468	25.074762598538587
88-89	24.088823920002014	24.77353954624656	25.868088451210635	25.269548082540787
90-91	24.098365627168175	24.69381584932709	25.902452248468784	25.305366275035958
92-93	24.146092323661748	24.6247584615198	25.997314544209495	25.23183467060896
94-95	24.13699306889058	24.45399501854079	26.079310035552446	25.32970187701618
96-97	24.113791944853098	24.415615495067083	26.05756795762817	25.413024602451646
98-99	24.04556982405156	24.392309531629383	25.964070002971894	25.598050641347157
100-101	24.253301464393004	24.220216975430723	26.02916655025828	25.497315009917994
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	48.0
1	43.0
2	55.5
3	77.0
4	75.5
5	93.0
6	140.0
7	270.5
8	413.0
9	450.0
10	402.0
11	367.0
12	417.5
13	466.0
14	530.5
15	630.5
16	704.5
17	778.5
18	863.5
19	939.5
20	1002.5
21	1082.5
22	1189.0
23	1307.5
24	1409.0
25	1583.5
26	1812.0
27	2135.5
28	2706.5
29	3591.0
30	5065.5
31	7583.5
32	11760.5
33	16761.5
34	22070.5
35	28572.5
36	36965.0
37	45400.5
38	53633.5
39	61456.0
40	68072.0
41	74655.5
42	81678.0
43	89679.0
44	97569.0
45	105141.0
46	110578.5
47	115288.5
48	121010.0
49	124345.0
50	124506.0
51	123391.0
52	122978.0
53	122985.5
54	122224.0
55	117715.0
56	109139.0
57	98441.0
58	89010.0
59	79569.0
60	66823.0
61	55014.0
62	45256.5
63	35159.0
64	25900.5
65	19271.0
66	14655.0
67	11558.0
68	9212.5
69	7203.5
70	5623.5
71	4323.0
72	3254.0
73	2484.0
74	1942.5
75	1563.5
76	1186.0
77	817.5
78	546.5
79	372.0
80	279.0
81	195.0
82	141.5
83	113.0
84	91.0
85	71.5
86	59.0
87	56.5
88	47.5
89	45.0
90	37.0
91	32.5
92	33.5
93	25.0
94	18.5
95	16.5
96	13.0
97	9.5
98	12.0
99	10.0
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.046143429036321265
2	0.06543710445214398
3	0.061182225463103236
4	0.06129226543695773
5	0.05740418636076532
6	0.009573477725341693
7	0.011884317176286237
8	0.015368916348345476
9	0.006308958500991461
10-11	0.01287467694097676
12-13	0.020944275023640255
14-15	0.03717517116717933
16-17	0.02558429392117176
18-19	0.010362097537965624
20-21	0.01942205538531964
22-23	0.03436915183388952
24-25	0.03235175231322365
26-27	0.020430755145652575
28-29	0.011902657171928656
30-31	0.012049377137067992
32-33	0.009353397777632689
34-35	0.005887138601215869
36-37	7.886198126239326E-4
38-39	0.0043282389716104205
40-41	0.0012837996949691924
42-43	0.20463767137808928
44-45	0.17639407808876703
46-47	0.18200611675534664
48-49	0.18910369506896205
50-51	0.18063061708216538
52-53	0.1871046355439386
54-55	0.18395015629344286
56-57	0.1867928556180175
58-59	0.19599953343051088
60-61	0.19913567268536417
62-63	0.18514225601019996
64-65	0.18563743589254522
66-67	0.18251963663333431
68-69	0.17844815760071775
70-71	0.17094709938296918
72-73	0.1816209768468559
74-75	0.18030049716060187
76-77	0.17490853844173126
78-79	0.19273501420616063
80-81	0.20285869180077482
82-83	0.20245521189664165
84-85	0.1958711534610139
86-87	0.2141194491252189
88-89	0.20773713064165777
90-91	0.2134592092820919
92-93	0.20911263031483907
94-95	0.2125238695043286
96-97	0.21949306784844708
98-99	0.21265224947382552
100-101	0.21912626793559875
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2726282.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.17300821145879
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.21050875455862	47.06161585510425
2	12.00509588114324	14.447654660707645
3	4.058948487181783	7.3271742194723295
4	1.8170236876358201	4.373431251061013
5	1.015990444880474	3.0567600691278214
6	0.6351692196571412	2.2932025602057022
7	0.42065525571736856	1.771846450953216
8	0.3181184940969694	1.5313717406011074
9	0.23625492310235227	1.2794552485051867
>10	1.2141496201304887	12.82407011711752
>50	0.05246798808320244	2.111505913259129
>100	0.014638673132792398	1.4416062259097873
>500	7.950886773240713E-4	0.3418557609096547
>1k	1.8348200245390544E-4	0.13844992706553447
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.667999128483407E-5
2	0.0	0.0	0.0	0.0	3.667999128483407E-5
3	0.0	0.0	0.0	0.0	3.667999128483407E-5
4	0.0	0.0	0.0	0.0	3.667999128483407E-5
5	0.0	0.0	0.0	0.0	3.667999128483407E-5
6	0.0	0.0	0.0	0.0	3.667999128483407E-5
7	0.0	0.0	0.0	0.0	3.667999128483407E-5
8	0.0	0.0	0.0	0.0	7.335998256966814E-5
9	0.0	0.0	0.0	0.0	1.8339995642417035E-4
10-11	0.0	0.0	0.0	3.667999128483407E-5	1.8339995642417035E-4
12-13	0.0	3.667999128483407E-5	0.0	7.335998256966814E-5	3.1177992592108957E-4
14-15	0.0	3.667999128483407E-5	0.0	7.335998256966814E-5	3.667999128483407E-4
16-17	0.0	3.667999128483407E-5	0.0	1.2837996949691924E-4	4.4015989541800886E-4
18-19	0.0	3.667999128483407E-5	0.0	1.4671996513933628E-4	4.7683988670284294E-4
20-21	0.0	3.667999128483407E-5	0.0	1.4671996513933628E-4	5.13519877987677E-4
22-23	0.0	3.667999128483407E-5	0.0	2.200799477090044E-4	6.235598518421792E-4
24-25	0.0	3.667999128483407E-5	0.0	5.13519877987677E-4	6.969198344118474E-4
26-27	0.0	3.667999128483407E-5	0.0	7.152598300542644E-4	7.702798169815154E-4
28-29	0.0	3.667999128483407E-5	0.0	0.002494239407368717	7.702798169815154E-4
30-31	0.0	3.667999128483407E-5	0.0	0.01100399738545022	7.702798169815154E-4
32-33	0.0	3.667999128483407E-5	0.0	0.035506231563719376	8.069598082663496E-4
34-35	0.0	3.667999128483407E-5	0.0	0.07016882332788757	8.436397995511836E-4
36-37	0.0	3.667999128483407E-5	0.0	0.1271511897888773	8.803197908360177E-4
38-39	0.0	3.667999128483407E-5	0.0	0.21718222839750254	8.986597864784347E-4
40-41	0.0	3.667999128483407E-5	0.0	0.3187307842695657	9.169997821208517E-4
42-43	0.0	3.667999128483407E-5	0.0	0.4261297987515598	9.169997821208517E-4
44-45	0.0	3.667999128483407E-5	0.0	0.564266645930245	9.536797734056859E-4
46-47	0.0	3.667999128483407E-5	0.0	0.7273458871826173	0.001045379751617771
48-49	0.0	3.667999128483407E-5	0.0	0.8985130665132954	0.0011003997385450222
50-51	0.0	3.667999128483407E-5	0.0	1.0773280240268615	0.001137079729829856
52-53	0.0	3.667999128483407E-5	0.0	1.2601961205774017	0.0012837996949691924
54-55	0.0	3.667999128483407E-5	0.0	1.475342609458596	0.0014671996513933628
56-57	0.0	3.667999128483407E-5	0.0	1.727810989472109	0.0014671996513933628
58-59	0.0	3.667999128483407E-5	0.0	1.9881105476249339	0.00148553964703578
60-61	0.0	3.667999128483407E-5	0.0	2.262476882435493	0.001503879642678197
62-63	0.0	3.667999128483407E-5	0.0	2.5454446752023454	0.0015405596339630309
64-65	0.0	3.667999128483407E-5	0.0	2.8625248598640933	0.0015405596339630309
66-67	0.0	3.667999128483407E-5	0.0	3.230241772494555	0.001558899629605448
68-69	0.0	3.667999128483407E-5	0.0	3.626917538244393	0.0016139196165326991
70-71	0.0	7.335998256966814E-5	0.0	4.046444938564683	0.0016139196165326991
72-73	0.0	7.335998256966814E-5	0.0	4.4826250549282864	0.0017973195729568694
74-75	0.0	7.335998256966814E-5	0.0	4.938373946642351	0.0018339995642417035
76-77	0.0	7.335998256966814E-5	0.0	5.460165162664758	0.0018339995642417035
78-79	0.0	7.335998256966814E-5	0.0	6.0381684653311725	0.0018339995642417035
80-81	0.0	7.335998256966814E-5	0.0	6.62407630611947	0.001962379533738623
82-83	0.0	7.335998256966814E-5	0.0	7.233165901399782	0.002054079511950708
84-85	0.0	7.335998256966814E-5	0.0	7.878898807973643	0.002054079511950708
86-87	0.0	7.335998256966814E-5	0.0	8.566135124686294	0.002054079511950708
88-89	0.0	7.335998256966814E-5	0.0	9.306942568670445	0.002054079511950708
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1930	0.0	45.74674	1
GTATCAA	3395	0.0	41.94556	1
TCAACGC	4200	0.0	33.118504	4
CAACGCA	4290	0.0	32.422516	5
ATCAACG	4380	0.0	31.646755	3
AACGCAG	4575	0.0	30.593433	6
TATCAAC	4640	0.0	30.282673	2
GTACATG	7565	0.0	28.23632	1
TACATGG	7960	0.0	25.762686	2
ACGCAGA	5480	0.0	25.024786	7
ACATGGG	8175	0.0	24.039919	3
CGCAGAG	5805	0.0	23.462408	8
CATGGGG	6685	0.0	21.233526	4
TAAGGCG	670	0.0	19.838993	5
GCAGAGT	6930	0.0	19.106792	9
AGAGTAC	5590	0.0	18.296173	10-11
TATACCG	235	2.544355E-6	18.180717	5
GAGTACA	5520	0.0	17.800648	1
CAGAGTA	6020	0.0	17.06814	10-11
AGTACAT	5635	0.0	16.174385	2
>>END_MODULE