FastQCFastQC Report
Mon 15 Aug 2016
SRR2921655_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921655_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2726282
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32950.044.4150851
GGTATCA17850.038.864211
TCAACGC42900.033.851124
CAACGCA44100.033.0594565
TATCAAC45900.031.9476412
ATCAACG45750.031.7412013
AACGCAG48750.029.99916
GTACATG72100.026.6244891
TACATGG76250.024.7705822
ACGCAGA59950.023.6808997
ACATGGG79850.022.7029063
CGCAGAG63350.021.8103478
CATGGGG68750.020.2947444
GCAGAGT72050.018.32049
AGAGTAC58850.017.91144610-11
TAAGGCG6550.016.6756365
TAAGGTA6350.016.4421234
GTATAAT8100.016.4251061
GTATTAA14550.015.6752121
GTATATA6150.015.452191
Produced by FastQC (version 0.11.5)