##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921655_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2726282
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.919799565855623	33.0	31.0	34.0	28.0	34.0
2	31.064559352260698	33.0	31.0	34.0	28.0	34.0
3	31.133831716601584	34.0	31.0	34.0	27.0	34.0
4	34.61565201252108	37.0	35.0	37.0	32.0	37.0
5	34.308759695438695	37.0	35.0	37.0	30.0	37.0
6	34.2705648205138	37.0	35.0	37.0	30.0	37.0
7	34.13694914906088	37.0	35.0	37.0	30.0	37.0
8	34.06865577368739	37.0	35.0	37.0	30.0	37.0
9	35.61151084150502	39.0	37.0	39.0	30.0	39.0
10-11	35.484523061077326	39.0	37.0	39.0	30.0	39.0
12-13	35.38883395041306	39.0	37.0	39.0	30.0	39.0
14-15	36.56829557617297	40.0	37.0	41.0	30.0	41.0
16-17	36.52080654165637	40.0	37.0	41.0	29.5	41.0
18-19	36.46874626322589	40.0	37.0	41.0	29.0	41.0
20-21	36.40029718128939	40.0	37.0	41.0	29.0	41.0
22-23	36.29890744977959	40.0	37.0	41.0	28.0	41.0
24-25	36.202694732239735	40.0	37.0	41.0	27.5	41.0
26-27	36.06251664354605	40.0	36.0	41.0	27.0	41.0
28-29	35.929640440717435	40.0	36.0	41.0	27.0	41.0
30-31	35.76857383058686	40.0	36.0	41.0	27.0	41.0
32-33	35.719320121689535	40.0	36.0	41.0	26.5	41.0
34-35	35.854116522061915	40.0	36.0	41.0	27.0	41.0
36-37	35.84496541443622	40.0	36.0	41.0	27.0	41.0
38-39	35.73353068391311	40.0	36.0	41.0	26.0	41.0
40-41	35.642790070873076	40.0	36.0	41.0	26.0	41.0
42-43	35.515288220367516	40.0	35.0	41.0	25.0	41.0
44-45	35.38093839888904	40.0	35.0	41.0	25.0	41.0
46-47	35.21792536502093	40.0	35.0	41.0	24.0	41.0
48-49	35.063268216567465	39.0	35.0	41.0	24.0	41.0
50-51	34.89051572801346	39.0	35.0	41.0	23.5	41.0
52-53	34.70706772080071	39.0	35.0	41.0	23.0	41.0
54-55	34.482087142856095	39.0	34.5	41.0	22.5	41.0
56-57	34.238327876573294	38.0	34.0	40.0	22.0	41.0
58-59	33.98280882168463	38.0	34.0	40.0	21.0	41.0
60-61	33.69032147077962	37.5	34.0	40.0	20.0	41.0
62-63	33.37774082064878	37.0	33.5	40.0	18.5	41.0
64-65	33.03244436929121	36.5	33.0	39.5	17.5	41.0
66-67	32.67235341024883	36.0	33.0	39.0	14.0	41.0
68-69	32.30831531734428	35.0	33.0	39.0	12.5	40.0
70-71	31.917645716767375	35.0	32.0	38.0	8.0	40.0
72-73	31.56133463082689	35.0	32.0	37.0	7.0	39.0
74-75	31.16564317264318	35.0	32.0	37.0	3.5	39.0
76-77	28.52779022859704	32.0	27.5	34.5	2.0	36.0
78-79	30.080686260628944	34.5	30.5	35.0	2.0	37.0
80-81	30.269383724794427	35.0	31.0	35.0	2.0	37.0
82-83	30.112137152356212	35.0	31.0	35.0	2.0	36.5
84-85	29.92444838795106	35.0	31.0	35.0	2.0	36.0
86-87	29.72602815849571	34.5	31.0	35.0	2.0	36.0
88-89	29.47211293622597	34.0	31.0	35.0	2.0	35.0
90-91	29.277819389190114	34.0	31.0	35.0	2.0	35.0
92-93	29.119858473921628	34.0	30.0	35.0	2.0	35.0
94-95	29.006663654016716	34.0	30.0	35.0	2.0	35.0
96-97	28.794045700334742	34.0	30.0	35.0	2.0	35.0
98-99	28.579931203008346	34.0	30.0	35.0	2.0	35.0
100-101	27.442157304343425	33.0	27.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	76719.0
3	23923.0
4	12874.0
5	8770.0
6	7473.0
7	7430.0
8	7603.0
9	8008.0
10	8698.0
11	9240.0
12	9691.0
13	10327.0
14	10716.0
15	11176.0
16	11940.0
17	12517.0
18	11887.0
19	10989.0
20	10475.0
21	10711.0
22	11223.0
23	12596.0
24	14914.0
25	17736.0
26	21504.0
27	26068.0
28	31280.0
29	37181.0
30	45673.0
31	56784.0
32	71431.0
33	94660.0
34	134515.0
35	203204.0
36	357792.0
37	658624.0
38	594407.0
39	55523.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.109669309710746	19.700877839350035	13.46079109127869	26.728661759660532
2	15.326480270699031	21.177027262473523	36.36027179943329	27.136220667394156
3	17.70170169729531	24.55742627684156	28.554391841948203	29.186480183914927
4	13.360199138562848	16.936755312085726	34.16795603457194	35.53508951477949
5	14.747258770521846	36.829202701845	33.74261151200814	14.680927015625016
6	34.531643916665786	35.00317471729243	16.367675912534065	14.097505453507722
7	29.548300579323783	31.128107803961587	20.66950520892556	18.65408640778907
8	26.999958184809934	34.481979487081674	19.525126160830027	18.992936167278366
9	26.757036417009104	15.117627273197442	18.731194620070347	39.39414168972311
10-11	25.9921690659758	25.00027522026795	27.49984404184816	21.507711671908083
12-13	26.37992366137813	22.356667011459596	27.268829178578528	23.994580148583744
14-15	23.672857552643684	23.96335795650876	24.224586395946236	28.139198094901325
16-17	24.126686087499387	26.92243502322944	25.034534211794675	23.916344677476506
18-19	24.19253257233406	26.38980794060684	26.17216385938142	23.24549562767768
20-21	24.729525882820074	25.546647397412777	25.93176960159045	23.792057118176707
22-23	24.585754518424725	25.326470262430668	25.734736905426516	24.3530383137181
24-25	24.403626347950258	25.53069781448566	25.675272689525503	24.390403148038583
26-27	24.231802859983542	25.6648024753829	25.757000254382973	24.34639441025059
28-29	24.318278192532077	25.812695883367958	25.63486019649963	24.234165727600335
30-31	24.60724225705902	25.59213800316712	25.398416587439787	24.402203152334074
32-33	24.38370913573091	25.66152288472438	25.366230793357904	24.58853718618681
34-35	24.624112210763485	25.64085351023638	25.494632637481917	24.24040164151822
36-37	24.665773966674696	25.510030219869407	25.482152449951606	24.342043363504295
38-39	24.559546664651712	25.584458981132546	25.48243358537378	24.373560768841962
40-41	24.638115222462506	25.4567153251337	25.546603056256224	24.358566396147573
42-43	24.59390083188816	25.4306862274867	25.376193013460064	24.599219927165077
44-45	24.42474386392232	25.431973999391065	25.47225146638592	24.671030670300688
46-47	24.59917616917382	25.466566330413055	25.359107743419194	24.575149756993934
48-49	24.35892474226672	25.501687346651746	25.422891311911794	24.716496599169744
50-51	24.402528503992926	25.540524034029822	25.442126889582816	24.61482057239444
52-53	24.615132365048503	25.42584914439522	25.39065401500497	24.568364475551306
54-55	24.271733369036724	25.354976679694737	25.539356568659638	24.8339333826089
56-57	24.259872508659452	25.405332039650524	25.504100687162	24.830694764528026
58-59	24.310555864019367	25.325038010751417	25.53794224126688	24.826463883962333
60-61	24.258216145854828	25.353304863887914	25.614393999003333	24.77408499125393
62-63	24.294026995381167	25.393088755373185	25.566494483870773	24.746389765374875
64-65	24.14678895716102	25.20705781766876	25.525143979994162	25.12100924517605
66-67	24.097020735918765	25.369098032767813	25.421360350700706	25.112520880612717
68-69	24.148267727862557	25.30897519831367	25.408847637557173	25.133909436266595
70-71	24.07388715790926	25.112560574601222	25.48816622885463	25.32538603863488
72-73	24.069136664950108	25.249544702115845	25.34125555626697	25.34006307666708
74-75	23.97985961460884	25.333980782357884	25.360837564713563	25.325322038319715
76-77	23.96225731971541	25.033223607976314	25.589428432645168	25.41509063966312
78-79	23.900132830009706	25.09195041331112	25.566886067752666	25.441030688926507
80-81	23.928179620318534	25.22028432616505	25.38535763926994	25.466178414246478
82-83	23.825268012893456	25.162567002076813	25.493543823976538	25.51862116105319
84-85	23.820340956652238	25.127905993154737	25.496720032162052	25.55503301803097
86-87	23.74441022621895	25.24185572689953	25.455509098620244	25.55822494826127
88-89	23.572353393387207	25.038051694394685	25.535206553204326	25.85438835901378
90-91	23.481213377678156	25.027831999009763	25.56059890158094	25.93035572173114
92-93	23.537061046753788	24.967348348261286	25.629996143086714	25.865594461898212
94-95	23.421846536029996	24.84695715460629	25.693791786896274	26.03740452246744
96-97	23.26709926782343	24.774832363536113	25.702282922770657	26.255785445869794
98-99	23.181296987516948	24.7196521529893	25.660521242319135	26.438529617174616
100-101	23.092221468855264	24.72228177631824	25.655715050492805	26.529781704333686
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	54.0
1	41.5
2	43.0
3	67.0
4	61.0
5	64.0
6	116.5
7	262.5
8	374.0
9	374.0
10	324.5
11	273.0
12	257.0
13	284.0
14	333.0
15	344.0
16	368.5
17	419.0
18	477.0
19	506.0
20	584.5
21	671.0
22	770.0
23	936.0
24	1067.0
25	1301.0
26	1695.5
27	2192.0
28	2872.5
29	3956.5
30	5820.0
31	8624.0
32	13060.0
33	18150.5
34	23192.5
35	29383.5
36	37596.5
37	46019.5
38	53988.0
39	61770.5
40	68818.0
41	75653.5
42	82341.0
43	89930.5
44	97805.0
45	105382.0
46	110711.5
47	115795.0
48	122228.0
49	125156.0
50	124598.5
51	123885.5
52	123708.5
53	123747.0
54	122516.0
55	117076.5
56	108509.0
57	98073.0
58	88399.5
59	78700.5
60	66039.5
61	54315.5
62	44640.0
63	34483.0
64	25300.0
65	18670.5
66	14159.5
67	11244.5
68	8718.5
69	6752.5
70	5247.0
71	3946.0
72	3020.5
73	2359.0
74	1789.0
75	1340.0
76	967.5
77	624.0
78	368.5
79	226.5
80	139.5
81	83.0
82	49.5
83	32.0
84	20.0
85	11.0
86	7.5
87	7.0
88	6.5
89	3.5
90	2.0
91	2.0
92	1.5
93	0.5
94	1.0
95	1.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1436388458714102
2	2.567599389938385E-4
3	0.008766517917075342
4	0.00513519877987677
5	0.021604514866767267
6	0.0020173995206658737
7	0.0
8	0.0
9	3.667999128483407E-5
10-11	0.04372254961152221
12-13	2.9343993027867256E-4
14-15	0.004548318919319424
16-17	0.0
18-19	0.001540559633963031
20-21	1.6505996078175332E-4
22-23	0.0
24-25	4.7683988670284294E-4
26-27	0.0028426993245746403
28-29	0.006235598518421792
30-31	0.01689113598666609
32-33	0.01236115706298908
34-35	0.009866917655620365
36-37	0.003502939167701654
38-39	0.0
40-41	0.0037963790979803264
42-43	0.009335057781990272
44-45	0.007042558326688141
46-47	0.004236538993398335
48-49	0.009408417764559938
50-51	0.003117799259210896
52-53	0.0019256995424537888
54-55	0.004291558980325586
56-57	0.007867858130596908
58-59	0.014983776439854716
60-61	0.007391018243894066
62-63	0.021769574827549022
64-65	0.039504350613766295
66-67	0.05742252635640774
68-69	0.047904068617993296
70-71	0.06415330475717479
72-73	0.03185657243087839
74-75	0.026171173781729107
76-77	0.20913097031048145
78-79	0.07788996149334515
80-81	0.06363978487918712
82-83	0.026171173781729107
84-85	0.04883940839575657
86-87	0.06512532452622288
88-89	0.279208093660157
90-91	0.2838481125576885
92-93	0.04786738862670846
94-95	0.009096637838638849
96-97	0.013516576788461356
98-99	9.536797734056859E-4
100-101	9.169997821208518E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2726282.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.76625473588162
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.54534547549825	47.72906471481954
2	11.823947278384091	14.36993984603844
3	3.9708019139427133	7.238722818251077
4	1.8010783504925922	4.377791433812574
5	1.0084770565469923	3.064068685671332
6	0.6363018537347563	2.319940831977594
7	0.42030981449100013	1.7878457278745885
8	0.30152061103087135	1.4657822606416486
9	0.23995526677782325	1.3123064579613875
>10	1.191205794145123	12.641458094749394
>50	0.04644629103183592	1.883683399903524
>100	0.013762562949582006	1.388862111285007
>500	6.055221245410984E-4	0.24814758187958808
>1k	2.422088497733872E-4	0.1723860351342977
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.667999128483407E-5
2	0.0	0.0	0.0	0.0	7.335998256966814E-5
3	0.0	0.0	0.0	0.0	7.335998256966814E-5
4	0.0	0.0	0.0	0.0	7.335998256966814E-5
5	0.0	0.0	0.0	0.0	7.335998256966814E-5
6	0.0	0.0	0.0	0.0	1.1003997385450222E-4
7	0.0	0.0	0.0	0.0	1.1003997385450222E-4
8	0.0	0.0	0.0	0.0	1.1003997385450222E-4
9	0.0	0.0	0.0	0.0	1.1003997385450222E-4
10-11	0.0	0.0	0.0	3.667999128483407E-5	1.1003997385450222E-4
12-13	0.0	0.0	0.0	3.667999128483407E-5	2.017399520665874E-4
14-15	1.8339995642417035E-5	3.667999128483407E-5	0.0	3.667999128483407E-5	4.2181989977559185E-4
16-17	3.667999128483407E-5	3.667999128483407E-5	0.0	5.50199869272511E-5	5.31859873630094E-4
18-19	3.667999128483407E-5	3.667999128483407E-5	0.0	1.1003997385450222E-4	5.501998692725111E-4
20-21	3.667999128483407E-5	3.667999128483407E-5	0.0	1.4671996513933628E-4	5.685398649149282E-4
22-23	3.667999128483407E-5	3.667999128483407E-5	0.0	2.200799477090044E-4	7.152598300542644E-4
24-25	3.667999128483407E-5	3.667999128483407E-5	0.0	5.31859873630094E-4	7.702798169815154E-4
26-27	3.667999128483407E-5	3.667999128483407E-5	0.0	7.702798169815154E-4	7.702798169815154E-4
28-29	3.667999128483407E-5	3.667999128483407E-5	0.0	0.0025492593942959677	7.702798169815154E-4
30-31	3.667999128483407E-5	3.667999128483407E-5	0.0	0.010875617415953302	7.886198126239325E-4
32-33	3.667999128483407E-5	5.50199869272511E-5	0.0	0.03501105168137412	8.436397995511836E-4
34-35	3.667999128483407E-5	7.335998256966814E-5	0.0	0.06884834364163356	9.536797734056859E-4
36-37	3.667999128483407E-5	7.335998256966814E-5	0.0	0.125115450272569	9.536797734056859E-4
38-39	3.667999128483407E-5	7.335998256966814E-5	0.0	0.21274394945203762	9.9035976469052E-4
40-41	3.667999128483407E-5	7.335998256966814E-5	0.0	0.31174324592980474	9.9035976469052E-4
42-43	5.50199869272511E-5	7.335998256966814E-5	0.0	0.41749166080398137	9.9035976469052E-4
44-45	7.335998256966814E-5	7.335998256966814E-5	0.0	0.5526024087016677	0.0010086997603329369
46-47	7.335998256966814E-5	7.335998256966814E-5	0.0	0.7116285109170658	0.001027039755975354
48-49	7.335998256966814E-5	7.335998256966814E-5	0.0	0.8796228710016059	0.001063719747260188
50-51	7.335998256966814E-5	7.335998256966814E-5	0.0	1.0548615293649006	0.0011003997385450222
52-53	7.335998256966814E-5	7.335998256966814E-5	0.0	1.2339882668043878	0.0012287797080419412
54-55	7.335998256966814E-5	7.335998256966814E-5	0.0	1.4446414567531898	0.0013571596775388607
56-57	1.1003997385450222E-4	7.335998256966814E-5	0.0	1.6909109182395659	0.0013754996731812777
58-59	1.1003997385450222E-4	7.335998256966814E-5	0.0	1.946038597621229	0.001430519660108529
60-61	1.1003997385450222E-4	7.335998256966814E-5	0.0	2.2153247536388383	0.0014671996513933628
62-63	1.1003997385450222E-4	7.335998256966814E-5	0.0	2.492001927900342	0.0014671996513933628
64-65	1.1003997385450222E-4	7.335998256966814E-5	0.0	2.803176633965232	0.0015222196383206138
66-67	1.1003997385450222E-4	7.335998256966814E-5	0.0	3.1629706684781693	0.001558899629605448
68-69	1.1003997385450222E-4	7.335998256966814E-5	0.0	3.5511183362542837	0.001577239625247865
70-71	1.1003997385450222E-4	7.335998256966814E-5	0.0	3.9614390587620796	0.0016139196165326991
72-73	1.1003997385450222E-4	7.335998256966814E-5	0.0	4.388467517300118	0.0017606395816720352
74-75	1.1003997385450222E-4	7.335998256966814E-5	0.0	4.837338910648275	0.0018523395598841206
76-77	1.1003997385450222E-4	7.335998256966814E-5	0.0	5.352179268322206	0.0018706795555265376
78-79	1.1003997385450222E-4	7.335998256966814E-5	0.0	5.9216361330192555	0.0018706795555265376
80-81	1.1003997385450222E-4	7.335998256966814E-5	0.0	6.4998595156333785	0.0019073595468113718
82-83	1.1003997385450222E-4	7.335998256966814E-5	0.0	7.099705753109913	0.002054079511950708
84-85	1.1003997385450222E-4	7.335998256966814E-5	0.0	7.738946301226359	0.002054079511950708
86-87	1.1003997385450222E-4	7.335998256966814E-5	0.0	8.417636179969644	0.002054079511950708
88-89	1.1003997385450222E-4	7.335998256966814E-5	0.0	9.151180985679398	0.002054079511950708
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3295	0.0	44.415085	1
GGTATCA	1785	0.0	38.86421	1
TCAACGC	4290	0.0	33.85112	4
CAACGCA	4410	0.0	33.059456	5
TATCAAC	4590	0.0	31.947641	2
ATCAACG	4575	0.0	31.741201	3
AACGCAG	4875	0.0	29.9991	6
GTACATG	7210	0.0	26.624489	1
TACATGG	7625	0.0	24.770582	2
ACGCAGA	5995	0.0	23.680899	7
ACATGGG	7985	0.0	22.702906	3
CGCAGAG	6335	0.0	21.810347	8
CATGGGG	6875	0.0	20.294744	4
GCAGAGT	7205	0.0	18.3204	9
AGAGTAC	5885	0.0	17.911446	10-11
TAAGGCG	655	0.0	16.675636	5
TAAGGTA	635	0.0	16.442123	4
GTATAAT	810	0.0	16.425106	1
GTATTAA	1455	0.0	15.675212	1
GTATATA	615	0.0	15.45219	1
>>END_MODULE