FastQCFastQC Report
Mon 15 Aug 2016
SRR2921654_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921654_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3247286
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23100.044.998611
GTATCAA39250.040.8737031
CAACGCA44100.036.2703365
TCAACGC44650.035.8257644
ATCAACG46700.034.149893
AACGCAG49600.032.4228256
TATCAAC51250.031.2111452
GTACATG71850.030.0574111
TACATGG76800.027.6261232
ACGCAGA59050.027.0779887
ACATGGG78800.025.9005643
CGCAGAG60750.025.6185198
CATGGGG68000.022.6861864
GCAGAGT71000.020.5841149
GAGTACA54300.020.191991
CAGAGTA65200.017.79403510-11
AGAGTAC58150.017.62573210-11
TATACCG2453.793628E-617.435595
GTATATG8300.017.1557671
GTACCGT3702.7976057E-916.6676526
Produced by FastQC (version 0.11.5)