##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921654_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3247286
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.30750201860877	31.0	31.0	34.0	26.0	34.0
2	30.355972649159945	31.0	31.0	34.0	26.0	34.0
3	30.416837937896446	33.0	31.0	34.0	26.0	34.0
4	33.87199772363753	37.0	35.0	37.0	30.0	37.0
5	33.86624461165416	37.0	35.0	37.0	30.0	37.0
6	33.914959446134404	37.0	35.0	37.0	30.0	37.0
7	33.81535904136562	37.0	35.0	37.0	29.0	37.0
8	33.78409323970848	37.0	35.0	37.0	29.0	37.0
9	35.33893873222131	39.0	37.0	39.0	29.0	39.0
10-11	35.2358611160212	39.0	35.0	39.0	29.0	39.0
12-13	35.124229125491254	39.0	35.0	39.0	28.0	39.0
14-15	36.25977077473311	40.0	37.0	41.0	27.0	41.0
16-17	36.19860846873358	40.0	36.5	41.0	27.0	41.0
18-19	36.16163821110922	40.0	36.5	41.0	27.0	41.0
20-21	36.08272477385731	40.0	36.5	41.0	27.0	41.0
22-23	35.984470262243605	40.0	36.0	41.0	27.0	41.0
24-25	35.87174351135071	40.0	36.0	41.0	27.0	41.0
26-27	35.722049736302864	40.0	36.0	41.0	26.5	41.0
28-29	35.581860513672034	40.0	36.0	41.0	25.5	41.0
30-31	35.436802455958606	40.0	36.0	41.0	25.0	41.0
32-33	35.255214970285955	39.0	35.0	41.0	25.0	41.0
34-35	35.08128942138143	39.0	35.0	41.0	24.0	41.0
36-37	35.04883354900061	39.0	35.0	41.0	24.0	41.0
38-39	35.226799548915615	39.5	35.0	41.0	24.5	41.0
40-41	35.28135187353377	40.0	35.0	41.0	24.0	41.0
42-43	35.11634885254948	40.0	35.0	41.0	23.5	41.0
44-45	34.99299107008129	40.0	35.0	41.0	23.0	41.0
46-47	34.83368973351901	40.0	35.0	41.0	22.0	41.0
48-49	34.66336981097446	39.0	35.0	41.0	20.5	41.0
50-51	32.9561515370066	37.0	32.5	39.5	17.0	40.0
52-53	33.071072427867456	37.5	32.5	39.5	16.0	40.0
54-55	33.98044505473186	38.5	34.0	40.5	18.0	41.0
56-57	34.00145690893873	39.0	34.0	41.0	17.5	41.0
58-59	33.80749647551832	38.0	34.0	41.0	14.5	41.0
60-61	33.553732871080655	38.0	34.0	40.0	13.0	41.0
62-63	33.26040838410907	37.0	33.5	40.0	10.5	41.0
64-65	32.961780237404405	37.0	33.0	40.0	8.0	41.0
66-67	32.620372982238095	36.0	33.0	39.0	6.5	41.0
68-69	32.25440475523253	36.0	33.0	39.0	2.0	41.0
70-71	31.845623391348962	35.0	32.0	39.0	2.0	40.5
72-73	31.48255866591363	35.0	32.0	37.5	2.0	40.0
74-75	31.083307260278275	35.0	32.0	37.0	2.0	39.0
76-77	30.70229801132392	35.0	31.0	36.5	2.0	39.0
78-79	30.35111135883935	35.0	31.0	36.0	2.0	38.5
80-81	30.030214924093535	35.0	31.0	35.5	2.0	37.0
82-83	29.704036848001685	35.0	31.0	35.0	2.0	37.0
84-85	29.428122130295883	35.0	31.0	35.0	2.0	36.5
86-87	29.17483015047027	35.0	30.0	35.0	2.0	36.0
88-89	28.92621176576378	34.5	30.0	35.0	2.0	36.0
90-91	28.676224699641487	34.0	30.0	35.0	2.0	35.5
92-93	28.459314024080417	34.0	30.0	35.0	2.0	35.0
94-95	28.201406035686418	34.0	29.0	35.0	2.0	35.0
96-97	27.93542361221032	34.0	29.0	35.0	2.0	35.0
98-99	27.62242100018292	34.0	29.0	35.0	2.0	35.0
100-101	26.51405958699049	33.0	26.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	117343.0
3	20697.0
4	13610.0
5	10260.0
6	9287.0
7	9689.0
8	10176.0
9	10883.0
10	11769.0
11	12547.0
12	12545.0
13	12685.0
14	13568.0
15	14220.0
16	14708.0
17	15355.0
18	14387.0
19	13800.0
20	13977.0
21	14710.0
22	16310.0
23	18711.0
24	21973.0
25	26031.0
26	30556.0
27	36066.0
28	42728.0
29	50160.0
30	59523.0
31	72528.0
32	89861.0
33	115833.0
34	159238.0
35	232541.0
36	386925.0
37	688460.0
38	707863.0
39	125747.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.13530933790639	17.480714329288773	11.537449540526868	25.84652679227797
2	16.699922497222687	19.588110488452397	37.75873678177037	25.95323023255456
3	19.44753366324033	22.769930699808977	29.426342084527807	28.356193552422887
4	13.354758073412759	14.80849314578828	35.8869429469998	35.949805833799154
5	14.127045607703678	36.8620903969948	34.63740102246105	14.373462972840473
6	34.58192496685366	34.80062150346554	16.352717075792317	14.264736453888476
7	29.46790047599908	31.435250419251858	20.709455765656305	18.387393339092753
8	27.23894525975498	34.20978873655888	19.79258040035087	18.75868560333527
9	26.846447816316243	14.72101369269853	18.9467145499974	39.48582394098783
10-11	25.925645882807498	24.69465447250562	27.984084288347383	21.395615356339498
12-13	26.170801146691	22.234156225098637	27.662934818064294	23.932107810146068
14-15	23.177708177918436	23.993708594429076	24.5248828910398	28.303700336612685
16-17	23.717888592407775	27.11067986585192	25.564218964780355	23.607212576959952
18-19	23.82121160044817	26.38622743979171	26.77728336933604	23.01527759042408
20-21	24.08464432476786	25.709764969450543	26.67159589486976	23.53399481091184
22-23	24.001025844820177	25.615437578266874	26.388333094585665	23.99520348232728
24-25	23.777321787840634	25.796206794893557	26.4339269656916	23.992544451574208
26-27	23.60663099756191	25.970118590199192	26.464558851635733	23.95869156060316
28-29	23.784029091213718	26.19858504643365	26.32504448101276	23.692341381339872
30-31	23.96363089264405	25.946105433945515	26.093200649916927	23.997063023493503
32-33	23.664480896363443	26.039560065925745	26.09998532485429	24.19597371285652
34-35	24.045342690211204	26.000598381106556	26.214389510376122	23.73966941830612
36-37	24.124216031844675	25.826727255146025	26.230313414809153	23.818743298200147
38-39	23.90204220404117	25.935161365177223	26.283928369878417	23.878868060903187
40-41	23.993369144224808	25.80284259601145	26.406354559852772	23.79743369991097
42-43	23.870228405672574	25.750633517142425	26.22059590811428	24.15854216907072
44-45	23.783779447483262	25.779784413814895	26.41020245453289	24.026233684168954
46-47	23.986317036884355	25.854965342860144	26.169808645562842	23.988908974692656
48-49	23.723430567185382	26.00091742567209	26.148298017999505	24.127353989143025
50-51	23.77833634232039	26.011764825676742	26.227186905151644	23.982711926851223
52-53	24.06225708790305	25.78545756731645	26.220126537241097	23.932158807539405
54-55	23.49416123171412	25.71162495618301	26.529783827951043	24.264429984151825
56-57	23.50695358930244	25.94086799173441	26.37213756623063	24.180040852732517
58-59	23.597482949142222	25.69524649813394	26.457564615502598	24.24970593722124
60-61	23.481458323431724	25.73462740423935	26.583383072846424	24.2005311994825
62-63	23.60684101169509	25.69398554857957	26.601191494666537	24.097981945058805
64-65	23.389667538343307	25.628280648300304	26.552327830234894	24.429723983121495
66-67	23.335816264722865	25.765437656771045	26.417855136073175	24.480890942432918
68-69	23.592453549756073	25.56467396351862	26.511019484773097	24.33185300195221
70-71	23.44456362226387	25.475346466273095	26.49629911990617	24.58379079155686
72-73	23.45783333281906	25.68897473795293	26.361256221400076	24.491935707827935
74-75	23.447114293529665	25.801765981051645	26.372370432985182	24.378749292433508
76-77	23.51615541247161	25.541461596502756	26.452643428526823	24.489739562498812
78-79	23.466252036351438	25.596046507967806	26.443776641525307	24.493924814155445
80-81	23.641693112385237	25.683541774204873	26.321525927606242	24.353239185803645
82-83	23.579282506532895	25.733540608263656	26.31254318870691	24.37463369649654
84-85	23.569110800683816	25.71927329152389	26.344794745854326	24.366821161937967
86-87	23.64175523003738	25.830522265248874	26.227886423023445	24.299836081690305
88-89	23.512068501215687	25.592522089512393	26.23856750544184	24.656841903830085
90-91	23.509292982639447	25.605841475345397	26.298615362794948	24.586250179220208
92-93	23.710080611184452	25.480582109236998	26.45600614591992	24.353331133658635
94-95	23.67993150181771	25.274971186439632	26.494940727712933	24.550156584029725
96-97	23.792875447823437	25.131700065255814	26.430719869809398	24.644704617111344
98-99	23.71283706669963	25.196522407991555	26.346202609104562	24.74443791620425
100-101	23.9104639601527	25.048146866541117	26.443410492822032	24.59797868048415
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	68.0
1	58.5
2	107.5
3	175.5
4	155.5
5	149.0
6	237.0
7	418.5
8	671.0
9	764.0
10	672.0
11	629.5
12	681.5
13	773.0
14	892.5
15	1000.5
16	1093.5
17	1187.5
18	1335.5
19	1462.5
20	1578.0
21	1760.0
22	1932.5
23	2199.5
24	2614.0
25	3235.5
26	4096.0
27	5383.5
28	7436.5
29	10537.0
30	14937.0
31	20845.0
32	29987.0
33	35786.0
34	38896.0
35	45593.5
36	54647.0
37	63641.5
38	72198.5
39	80057.5
40	86855.0
41	93845.0
42	100800.0
43	109014.0
44	116652.5
45	125602.5
46	131641.0
47	135393.5
48	140073.0
49	141873.5
50	141523.0
51	140428.0
52	139089.0
53	137710.0
54	135634.0
55	129184.0
56	118885.0
57	107404.0
58	97611.5
59	86123.0
60	70787.5
61	57710.5
62	47620.5
63	36765.0
64	26634.5
65	19811.0
66	15090.0
67	11762.0
68	9032.0
69	6846.0
70	5303.0
71	3948.5
72	2818.5
73	2051.0
74	1587.0
75	1206.0
76	818.5
77	553.5
78	374.5
79	252.0
80	186.5
81	148.5
82	124.5
83	105.0
84	85.5
85	72.5
86	60.5
87	42.5
88	43.0
89	46.5
90	40.0
91	27.5
92	23.0
93	25.5
94	20.5
95	13.5
96	13.5
97	11.0
98	8.0
99	8.0
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.049641454433025
2	0.06901147604491874
3	0.06556244199001873
4	0.0655316470431
5	0.06094319995220625
6	0.00988517796091875
7	0.01327262212198125
8	0.016444501654612496
9	0.0069904529505562495
10-11	0.013288019595440622
12-13	0.022387926409931248
14-15	0.03912498006027187
16-17	0.026899386133528123
18-19	0.009484843650975
20-21	0.02049403717442812
22-23	0.03636883231104375
24-25	0.03427477592056875
26-27	0.021109936112803124
28-29	0.012164004032906248
30-31	0.012610530763228121
32-33	0.009223086602165623
34-35	0.005897232334940624
36-37	7.390787260499999E-4
38-39	0.004080330466734374
40-41	0.0013241827175062499
42-43	0.22294001821829057
44-45	0.19283795760521247
46-47	0.1991663191970156
48-49	0.20621836204140934
50-51	0.1982886632098312
52-53	0.2050635515319562
54-55	0.20121418316711245
56-57	0.2039087410225031
58-59	0.21268530089434684
60-61	0.21778186460939997
62-63	0.20221501894197186
64-65	0.20313886734953435
66-67	0.19995159034344373
68-69	0.1953939381994687
70-71	0.1877721888370781
72-73	0.19881217730744996
74-75	0.19784213647950935
76-77	0.19155996730808436
78-79	0.2096827935697687
80-81	0.22044562751787183
82-83	0.22002989573446874
84-85	0.21400948361185312
86-87	0.23288678607304683
88-89	0.2261426926978406
90-91	0.23270201639153434
92-93	0.22828294150869372
94-95	0.23071574231527497
96-97	0.23798334978809998
98-99	0.2314394235678656
100-101	0.23770619526583123
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3247286.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.143119555538256
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.61550004007667	45.709504177486004
2	11.608374422903397	13.498942038326492
3	3.916600914810769	6.831701857235191
4	1.7499814242561034	4.0699751668197495
5	1.0391173236544913	3.0208761390737013
6	0.6441571340862098	2.247198315583643
7	0.4356790250419885	1.7732216348599679
8	0.31699826626339295	1.4745014475400644
9	0.24366899181947257	1.275090779100336
>10	1.3379126982549654	13.797466515731086
>50	0.06648264049893723	2.617421561343275
>100	0.02334793761650227	2.3380364597209446
>500	0.0013287687315730818	0.5342597713558335
>1k	8.504119855123099E-4	0.8118041358238505
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.079494691875E-5	0.0	0.0	0.0
3	0.0	3.079494691875E-5	0.0	0.0	0.0
4	0.0	3.079494691875E-5	0.0	0.0	0.0
5	0.0	3.079494691875E-5	0.0	0.0	0.0
6	0.0	3.079494691875E-5	0.0	0.0	0.0
7	0.0	3.079494691875E-5	0.0	0.0	0.0
8	0.0	3.079494691875E-5	0.0	0.0	0.0
9	0.0	3.079494691875E-5	0.0	0.0	0.0
10-11	0.0	3.079494691875E-5	0.0	0.0	0.0
12-13	0.0	3.079494691875E-5	0.0	0.0	6.15898938375E-5
14-15	0.0	3.079494691875E-5	0.0	0.0	1.23179787675E-4
16-17	0.0	3.079494691875E-5	0.0	0.0	1.847696815125E-4
18-19	0.0	3.079494691875E-5	0.0	3.079494691875E-5	2.1556462843124998E-4
20-21	0.0	3.079494691875E-5	0.0	3.079494691875E-5	2.1556462843124998E-4
22-23	0.0	3.079494691875E-5	0.0	9.238484075625E-5	2.3096210189062498E-4
24-25	0.0	4.6192420378125E-5	0.0	4.7732167724062497E-4	2.4635957535E-4
26-27	0.0	6.15898938375E-5	0.0	0.001108618089075	2.4635957535E-4
28-29	0.0	6.15898938375E-5	0.0	0.006975055477096874	2.4635957535E-4
30-31	0.0	6.15898938375E-5	0.0	0.029024237470921872	2.4635957535E-4
32-33	1.5397473459375E-5	6.15898938375E-5	0.0	0.07246051009981874	2.4635957535E-4
34-35	3.079494691875E-5	9.238484075625E-5	0.0	0.13274161869327186	3.0794946918749996E-4
36-37	3.079494691875E-5	1.23179787675E-4	0.0	0.22531122913103435	3.0794946918749996E-4
38-39	3.079494691875E-5	1.23179787675E-4	0.0	0.3718489840439062	3.0794946918749996E-4
40-41	3.079494691875E-5	1.23179787675E-4	0.0	0.5375104009933218	3.0794946918749996E-4
42-43	3.079494691875E-5	1.23179787675E-4	0.0	0.7104548228890217	3.2334694264687496E-4
44-45	3.079494691875E-5	1.23179787675E-4	0.0	0.9232325086241249	3.3874441610624997E-4
46-47	4.6192420378125E-5	1.23179787675E-4	0.0	1.1735646321266435	3.3874441610624997E-4
48-49	6.15898938375E-5	1.23179787675E-4	0.0	1.429224281446106	3.3874441610624997E-4
50-51	6.15898938375E-5	1.23179787675E-4	0.0	1.696647600488531	3.3874441610624997E-4
52-53	6.15898938375E-5	1.23179787675E-4	0.0	1.9646560235224122	4.0033430994374994E-4
54-55	6.15898938375E-5	1.23179787675E-4	0.0	2.271512272094297	4.6192420378124996E-4
56-57	6.15898938375E-5	1.23179787675E-4	0.0	2.6294727350778464	4.6192420378124996E-4
58-59	6.15898938375E-5	1.23179787675E-4	0.0	2.997333773495775	4.6192420378124996E-4
60-61	6.15898938375E-5	1.23179787675E-4	0.0	3.3784982289826027	4.6192420378124996E-4
62-63	6.15898938375E-5	1.23179787675E-4	0.0	3.7728891141710337	4.927191507E-4
64-65	6.15898938375E-5	1.23179787675E-4	0.0	4.197027918082977	4.927191507E-4
66-67	6.15898938375E-5	1.23179787675E-4	0.0	4.695767480905593	4.927191507E-4
68-69	6.15898938375E-5	1.23179787675E-4	0.0	5.22574851737728	4.927191507E-4
70-71	6.15898938375E-5	1.5397473459375E-4	3.079494691875E-5	5.775700076925777	4.927191507E-4
72-73	6.15898938375E-5	1.847696815125E-4	3.079494691875E-5	6.334797735709143	6.005014649156249E-4
74-75	6.15898938375E-5	1.847696815125E-4	3.079494691875E-5	6.913973699883533	6.312964118343749E-4
76-77	6.15898938375E-5	1.847696815125E-4	3.079494691875E-5	7.571353431758089	6.466938852937499E-4
78-79	6.15898938375E-5	1.847696815125E-4	3.079494691875E-5	8.29047703220474	6.466938852937499E-4
80-81	6.15898938375E-5	1.847696815125E-4	3.079494691875E-5	9.016375520973515	6.928863056718749E-4
82-83	6.15898938375E-5	1.847696815125E-4	3.079494691875E-5	9.756131735855726	7.3907872605E-4
84-85	6.15898938375E-5	1.847696815125E-4	3.079494691875E-5	10.528607581839111	7.3907872605E-4
86-87	6.15898938375E-5	1.847696815125E-4	3.079494691875E-5	11.362688719133455	7.3907872605E-4
88-89	6.15898938375E-5	2.1556462843124998E-4	3.079494691875E-5	12.25241632550998	7.54476199509375E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2310	0.0	44.99861	1
GTATCAA	3925	0.0	40.873703	1
CAACGCA	4410	0.0	36.270336	5
TCAACGC	4465	0.0	35.825764	4
ATCAACG	4670	0.0	34.14989	3
AACGCAG	4960	0.0	32.422825	6
TATCAAC	5125	0.0	31.211145	2
GTACATG	7185	0.0	30.057411	1
TACATGG	7680	0.0	27.626123	2
ACGCAGA	5905	0.0	27.077988	7
ACATGGG	7880	0.0	25.900564	3
CGCAGAG	6075	0.0	25.618519	8
CATGGGG	6800	0.0	22.686186	4
GCAGAGT	7100	0.0	20.584114	9
GAGTACA	5430	0.0	20.19199	1
CAGAGTA	6520	0.0	17.794035	10-11
AGAGTAC	5815	0.0	17.625732	10-11
TATACCG	245	3.793628E-6	17.43559	5
GTATATG	830	0.0	17.155767	1
GTACCGT	370	2.7976057E-9	16.667652	6
>>END_MODULE