FastQCFastQC Report
Mon 15 Aug 2016
SRR2921654_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921654_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3247286
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22450.046.9977841
GTATCAA38050.039.8452721
TCAACGC46800.031.7368014
CAACGCA48000.030.9686645
ATCAACG49700.030.0782343
AACGCAG52100.028.8939826
GTACATG78100.028.8448351
TATCAAC53250.027.9834652
TACATGG80850.026.7655562
ACATGGG82500.025.6554153
CATGGGG68700.022.5868514
ACGCAGA65700.022.5514837
CGCAGAG67850.021.276968
GAGTACA56950.019.110961
AGAGTAC63750.018.43688610-11
ATGGGGG37200.017.6179545
TATACCG2453.7721147E-617.4459975
GTATATA9650.017.2377871
GCAGAGT78450.017.1916669
CAGAGTA70750.015.77371510-11
Produced by FastQC (version 0.11.5)