##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921654_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3247286
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.274712482978092	33.0	31.0	34.0	28.0	34.0
2	31.394972909685194	34.0	31.0	34.0	28.0	34.0
3	31.472944483485595	34.0	31.0	34.0	28.0	34.0
4	34.94179293108152	37.0	35.0	37.0	32.0	37.0
5	34.66651720852429	37.0	35.0	37.0	32.0	37.0
6	34.63739381132429	37.0	35.0	37.0	32.0	37.0
7	34.51068061143983	37.0	35.0	37.0	31.0	37.0
8	34.45293269517991	37.0	35.0	37.0	31.0	37.0
9	36.03620561909237	39.0	37.0	39.0	31.0	39.0
10-11	35.923907225911115	39.0	37.0	39.0	31.0	39.0
12-13	35.84180589575418	39.0	37.0	39.0	30.0	39.0
14-15	37.0774953299463	40.0	38.0	41.0	31.0	41.0
16-17	37.03561050674317	40.0	38.0	41.0	31.0	41.0
18-19	36.99185027127268	40.0	38.0	41.0	31.0	41.0
20-21	36.928503833662944	40.0	37.5	41.0	30.0	41.0
22-23	36.836562748091794	40.0	37.0	41.0	30.0	41.0
24-25	36.74886997942282	40.0	37.0	41.0	30.0	41.0
26-27	36.619113622883845	40.0	37.0	41.0	30.0	41.0
28-29	36.49550655532035	40.0	37.0	41.0	30.0	41.0
30-31	36.33398382526208	40.0	37.0	41.0	29.0	41.0
32-33	36.27647149034609	40.0	36.5	41.0	29.5	41.0
34-35	36.40076174380698	40.0	37.0	41.0	30.0	41.0
36-37	36.391804879520926	40.0	37.0	41.0	30.0	41.0
38-39	36.27825020648012	40.0	37.0	41.0	29.0	41.0
40-41	36.18635623717775	40.0	36.5	41.0	28.5	41.0
42-43	36.0581853584809	40.0	36.0	41.0	28.0	41.0
44-45	35.92153293550368	40.0	36.0	41.0	27.5	41.0
46-47	35.761693303269254	40.0	35.5	41.0	27.0	41.0
48-49	35.61299220333534	40.0	35.0	41.0	26.5	41.0
50-51	35.44896984743568	39.5	35.0	41.0	26.0	41.0
52-53	35.266746292134414	39.0	35.0	41.0	26.0	41.0
54-55	35.04706484122433	39.0	35.0	41.0	25.5	41.0
56-57	34.81436759804957	39.0	35.0	41.0	25.0	41.0
58-59	34.5666030032464	38.0	34.5	40.0	24.0	41.0
60-61	34.28371015056881	38.0	34.0	40.0	23.5	41.0
62-63	33.97523932292998	37.0	34.0	40.0	23.0	41.0
64-65	33.63289590137734	37.0	34.0	40.0	22.0	41.0
66-67	33.283111958724916	36.0	34.0	39.0	22.0	41.0
68-69	32.92170800477692	36.0	33.0	39.0	21.0	41.0
70-71	32.527492496811185	35.0	33.0	38.5	20.0	40.0
72-73	32.16764769102568	35.0	33.0	37.5	18.5	39.5
74-75	31.775254628018597	35.0	32.5	37.0	17.5	39.0
76-77	29.092137403357757	33.0	28.5	34.5	9.0	36.5
78-79	30.65754617856265	34.5	31.0	36.0	10.5	37.0
80-81	30.846303343776924	35.0	32.0	35.5	10.5	37.0
82-83	30.681284771344437	35.0	32.0	35.0	7.0	37.0
84-85	30.4908706840112	35.0	32.0	35.0	6.5	36.0
86-87	30.286664463801465	35.0	32.0	35.0	2.0	36.0
88-89	30.019515219786616	35.0	31.0	35.0	2.0	36.0
90-91	29.823756207491424	34.0	31.0	35.0	2.0	35.0
92-93	29.66907334309328	34.0	31.0	35.0	2.0	35.0
94-95	29.563543833219494	34.0	31.0	35.0	2.0	35.0
96-97	29.36295401760116	34.0	31.0	35.0	2.0	35.0
98-99	29.159565403232115	34.0	31.0	35.0	2.0	35.0
100-101	28.043162659525525	33.0	28.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	69945.0
3	23743.0
4	12938.0
5	8863.0
6	7709.0
7	7542.0
8	7910.0
9	8399.0
10	9290.0
11	9850.0
12	10553.0
13	11402.0
14	12273.0
15	13147.0
16	13913.0
17	14656.0
18	14013.0
19	12735.0
20	12245.0
21	11930.0
22	12903.0
23	14505.0
24	16862.0
25	20557.0
26	24385.0
27	29455.0
28	35577.0
29	42481.0
30	51718.0
31	64147.0
32	81296.0
33	106619.0
34	151499.0
35	227802.0
36	404694.0
37	773899.0
38	796438.0
39	99393.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.206098763535735	18.925395434455858	12.001386769613335	26.867119032395077
2	15.827374241634304	20.79571850771061	36.24683927682222	27.130067973832862
3	18.10659044425466	23.840733676646273	29.203394283134315	28.849281595964754
4	13.034197707546666	15.905815947761209	34.824022378197824	36.235963966494296
5	13.95423620642497	37.266708638987936	34.615737649172175	14.163317505414922
6	34.74306313897004	35.240781493573884	16.026734269149774	13.989421098306304
7	29.330400833188087	31.611536526194488	20.740550724512715	18.31751191610471
8	27.21133894581506	34.64767193280789	19.567386426696014	18.573602694681036
9	26.69885766109227	14.667391374640662	18.814270998695832	39.81947996557123
10-11	25.88821039269935	24.936008429510682	27.937188437767656	21.238592740022305
12-13	26.097882746538403	22.337435134034003	27.709619733234625	23.85506238619297
14-15	23.129701077382844	24.127998649883835	24.488332458714286	28.25396781401903
16-17	23.69586844150291	27.304420877067937	25.482899486355603	23.516811195073547
18-19	23.781508749141192	26.55403846905966	26.68464197056386	22.97981081123529
20-21	24.080356544828707	25.83932232462424	26.625396197276025	23.454924933271023
22-23	23.94778285620669	25.7074677130379	26.38851336161952	23.95623606913589
24-25	23.751719138499894	25.963549028629007	26.413204215484658	23.87152761738644
26-27	23.57013569435088	26.11566163025695	26.452707150418632	23.861495524973535
28-29	23.752131903625944	26.321364492232757	26.310693425329323	23.615810178811973
30-31	23.932478441359077	26.0836916943407	26.091899963224492	23.891929901075727
32-33	23.63929987675882	26.166552531649174	26.064346894893582	24.12980069669842
34-35	23.97202970361828	26.136449206323054	26.186219074579192	23.705302015479475
36-37	24.070962514524272	26.001731979818615	26.18550846572888	23.741797039928233
38-39	23.860082521231966	26.08235401537684	26.245613451604427	23.811950011786767
40-41	23.920389016264956	25.95057534803436	26.347829568254156	23.781206067446526
42-43	23.87407891503074	25.959643805054906	26.096847969162784	24.069429310751573
44-45	23.735490586782383	25.938904167808737	26.313523435262375	24.012081810146505
46-47	23.929281446041895	25.982541324270624	26.124527220533306	23.963650009154172
48-49	23.639300352620136	26.136129327619507	26.13676068381728	24.087809635943078
50-51	23.737813279329583	26.112610873058546	26.162531080795592	23.987044766816272
52-53	23.986412358526866	25.906488189650485	26.119917202908155	23.987182248914493
54-55	23.400371401470824	25.878121188977442	26.373815888345508	24.347691521206226
56-57	23.445890645631945	26.02843382955871	26.232683120252847	24.292992404556497
58-59	23.44494269793924	25.864566661394782	26.29476138784037	24.3957292528256
60-61	23.354360715434012	25.973995976638385	26.3814906887389	24.290152619188703
62-63	23.47419264768867	25.881214550245033	26.35982522555627	24.28476757651002
64-65	23.172947975525215	25.781488518164004	26.32582788475372	24.71973562155706
66-67	23.1582113090373	25.92244852115659	26.204488074176435	24.71485209562968
68-69	23.347201270883556	25.724854696304185	26.251580593637698	24.67636343917457
70-71	23.18728748427607	25.601238068765575	26.222383356332436	24.98909109062592
72-73	23.093316443600095	25.749721940136226	26.140276484152707	25.016685132110972
74-75	22.95679733758341	25.787001128027764	26.162697212335978	25.09350432205285
76-77	22.94756806306153	25.501495365755044	26.38263462149808	25.16830194968534
78-79	22.819822475678205	25.572175291668124	26.308960248809587	25.299041983844084
80-81	22.867903759567174	25.60253503687581	26.204800434873583	25.32476076868343
82-83	22.703316024860868	25.62285396041815	26.219594275112613	25.45423573960837
84-85	22.634035722318593	25.505840232438477	26.288979815688364	25.571144229554566
86-87	22.600621246252032	25.638826658120422	26.188041009648423	25.572511085979126
88-89	22.43057520227022	25.427056089603024	26.19370006560115	25.948668642525607
90-91	22.299886661499333	25.349154084610536	26.27014715781973	26.0808120960704
92-93	22.30712657362415	25.181283165922743	26.478495741790535	26.033094518662576
94-95	22.092401213184388	24.99548813178474	26.618744364014447	26.293366291016422
96-97	22.06584872800945	24.873872762599593	26.57159519597711	26.488683313413848
98-99	21.9059212708318	24.899230397082587	26.518904097270962	26.67594423481465
100-101	21.748715040565468	24.786271224099295	26.559107980013437	26.905905755321797
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	61.0
1	51.0
2	77.0
3	127.5
4	116.0
5	117.5
6	229.0
7	436.0
8	613.5
9	638.5
10	533.5
11	466.5
12	479.0
13	507.5
14	568.0
15	612.0
16	661.5
17	720.0
18	771.5
19	861.0
20	1015.0
21	1207.5
22	1389.0
23	1718.5
24	2145.5
25	2690.5
26	3605.0
27	5140.5
28	7532.5
29	10806.0
30	15334.0
31	21349.5
32	30445.5
33	36372.5
34	39283.0
35	45465.0
36	54145.0
37	62906.5
38	71227.5
39	79668.0
40	86738.0
41	93526.0
42	100525.5
43	109114.5
44	117252.0
45	125518.5
46	131525.0
47	135650.5
48	141014.5
49	143065.5
50	142335.0
51	141391.0
52	140555.0
53	139951.5
54	138175.0
55	131336.5
56	120603.5
57	108743.5
58	98356.5
59	86738.0
60	70921.0
61	57112.0
62	47042.0
63	36138.0
64	25921.5
65	19083.5
66	14289.5
67	11170.5
68	8629.5
69	6457.5
70	4828.0
71	3505.5
72	2493.5
73	1792.5
74	1293.5
75	907.5
76	602.5
77	369.0
78	203.0
79	121.0
80	80.0
81	44.0
82	25.5
83	23.5
84	16.0
85	9.5
86	6.5
87	4.0
88	4.0
89	4.0
90	2.5
91	1.5
92	1.0
93	0.0
94	0.5
95	0.5
96	0.5
97	0.5
98	1.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16071882796895623
2	2.1556462843124995E-4
3	0.009146099234868749
4	0.0060666045429937495
5	0.021618052736962497
6	0.002094056390475
7	0.0
8	0.0
9	3.079494691874999E-5
10-11	0.047608987936387494
12-13	4.0033430994374994E-4
14-15	0.0048656016131625
16-17	1.5397473459374996E-5
18-19	0.0016321321866937495
20-21	2.61757048809375E-4
22-23	0.0
24-25	6.158989383749999E-4
26-27	0.0030333022714968748
28-29	0.005789450020725
30-31	0.017245170274499996
32-33	0.012333376240959372
34-35	0.010516474372753123
36-37	0.004157317834031249
38-39	1.5397473459374996E-5
40-41	0.00372618857716875
42-43	0.009238484075625
44-45	0.007883506411199998
46-47	0.004080330466734374
48-49	0.009469446177515623
50-51	0.0032026744795499995
52-53	0.0021710437577718744
54-55	0.0038801633117624993
56-57	0.008422417982278125
58-59	0.015412870932834374
60-61	0.007698736729687499
62-63	0.02160265526350312
64-65	0.04104966424269375
66-67	0.061482111523284366
68-69	0.05142756135431249
70-71	0.06873432152265
72-73	0.03487527738548437
74-75	0.0280849915899
76-77	0.22406403378082496
78-79	0.08365447330478436
80-81	0.06790285795584373
82-83	0.027684657279956247
84-85	0.052228229974199986
86-87	0.06762570343357499
88-89	0.29372220371103747
90-91	0.2968478908232906
92-93	0.05151994619506875
94-95	0.009053714394112499
96-97	0.014335047790678122
98-99	8.314635668062499E-4
100-101	1.38577261134375E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3247286.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.222925731903054
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.921657145186	44.58905199725792
2	11.97806797857332	13.708401886991743
3	3.934626811671818	6.754525734811527
4	1.8643446078960144	4.267330121452302
5	1.0654536575442313	3.048418775821901
6	0.6748325754600586	2.3169536608211874
7	0.46660947903866773	1.8690531695382158
8	0.33317006619611395	1.525197276322676
9	0.25888323029371935	1.333265027429939
>10	1.4054241318265217	14.172828329539849
>50	0.07010613887058403	2.6990371306405936
>100	0.024826235164613204	2.4485635233430796
>500	9.719719201859305E-4	0.38482221370610326
>1k	0.0010259703581565428	0.8825511523229354
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.15898938375E-5
2	0.0	0.0	0.0	0.0	6.15898938375E-5
3	0.0	0.0	0.0	0.0	6.15898938375E-5
4	0.0	0.0	0.0	0.0	6.15898938375E-5
5	0.0	3.079494691875E-5	0.0	0.0	6.15898938375E-5
6	0.0	3.079494691875E-5	0.0	0.0	6.15898938375E-5
7	0.0	3.079494691875E-5	0.0	0.0	6.15898938375E-5
8	0.0	3.079494691875E-5	0.0	0.0	6.15898938375E-5
9	0.0	3.079494691875E-5	0.0	3.079494691875E-5	6.15898938375E-5
10-11	0.0	3.079494691875E-5	0.0	4.6192420378125E-5	6.15898938375E-5
12-13	0.0	3.079494691875E-5	0.0	6.15898938375E-5	9.238484075625E-5
14-15	0.0	3.079494691875E-5	0.0	9.238484075625E-5	9.238484075625E-5
16-17	0.0	3.079494691875E-5	0.0	9.238484075625E-5	1.0778231421562499E-4
18-19	1.5397473459375E-5	3.079494691875E-5	0.0	9.238484075625E-5	1.5397473459375E-4
20-21	3.079494691875E-5	6.15898938375E-5	0.0	9.238484075625E-5	2.1556462843124998E-4
22-23	6.15898938375E-5	6.15898938375E-5	0.0	1.5397473459375E-4	2.3096210189062498E-4
24-25	6.15898938375E-5	6.15898938375E-5	0.0	5.2351409761875E-4	2.61757048809375E-4
26-27	6.15898938375E-5	6.15898938375E-5	0.0	0.0011702079829125	3.0794946918749996E-4
28-29	6.15898938375E-5	6.15898938375E-5	0.0	0.007067440317853124	3.3874441610624997E-4
30-31	6.15898938375E-5	9.238484075625E-5	0.0	0.029332186940109372	3.3874441610624997E-4
32-33	6.15898938375E-5	9.238484075625E-5	0.0	0.07355373071543436	3.69539363025E-4
34-35	6.15898938375E-5	9.238484075625E-5	0.0	0.13474329024299062	3.69539363025E-4
36-37	6.15898938375E-5	9.238484075625E-5	0.0	0.22811356930064058	3.69539363025E-4
38-39	6.15898938375E-5	9.238484075625E-5	0.0	0.37586772461680307	4.0033430994374994E-4
40-41	6.15898938375E-5	9.238484075625E-5	0.0	0.5422990152391874	4.3112925686249995E-4
42-43	6.15898938375E-5	9.238484075625E-5	0.0	0.7152126421879687	4.4652673032187496E-4
44-45	6.15898938375E-5	9.238484075625E-5	0.0	0.9289603687510124	4.7732167724062497E-4
46-47	6.15898938375E-5	9.238484075625E-5	0.0	1.1804165078160653	5.2351409761875E-4
48-49	6.15898938375E-5	9.238484075625E-5	0.0	1.4362763242904997	5.851039914562499E-4
50-51	6.15898938375E-5	9.238484075625E-5	0.0	1.7032069241822247	6.158989383749999E-4
52-53	6.15898938375E-5	9.238484075625E-5	0.0	1.971261539636484	6.312964118343749E-4
54-55	6.15898938375E-5	1.38577261134375E-4	0.0	2.277655864004587	6.466938852937499E-4
56-57	6.15898938375E-5	1.5397473459374998E-4	0.0	2.635231390151653	6.466938852937499E-4
58-59	6.15898938375E-5	1.847696815125E-4	0.0	3.0007828075506744	6.620913587531249E-4
60-61	6.15898938375E-5	1.847696815125E-4	0.0	3.380561490426159	6.774888322124999E-4
62-63	6.15898938375E-5	1.847696815125E-4	0.0	3.7726273571222246	7.0828377913125E-4
64-65	6.15898938375E-5	1.847696815125E-4	0.0	4.1959346974673615	7.3907872605E-4
66-67	6.15898938375E-5	1.847696815125E-4	0.0	4.692826563474853	7.3907872605E-4
68-69	6.15898938375E-5	2.4635957535E-4	0.0	5.220944505657956	7.3907872605E-4
70-71	6.15898938375E-5	2.61757048809375E-4	0.0	5.7714965666713685	8.006686198874999E-4
72-73	6.15898938375E-5	2.7715452226874994E-4	0.0	6.332857654053262	8.468610402656249E-4
74-75	6.15898938375E-5	2.7715452226874994E-4	0.0	6.916314115849358	8.776559871843749E-4
76-77	6.15898938375E-5	2.7715452226874994E-4	0.0	7.5758033015878485	8.930534606437499E-4
78-79	6.15898938375E-5	2.7715452226874994E-4	0.0	8.298699283032047	9.238484075624999E-4
80-81	6.15898938375E-5	2.7715452226874994E-4	0.0	9.029432578467064	9.392458810218748E-4
82-83	6.15898938375E-5	2.7715452226874994E-4	0.0	9.775224602945352	0.00101623324831875
84-85	6.15898938375E-5	2.7715452226874994E-4	0.0	10.553212744427192	0.00101623324831875
86-87	6.15898938375E-5	2.7715452226874994E-4	0.0	11.392482830277345	0.00101623324831875
88-89	6.15898938375E-5	2.7715452226874994E-4	0.0	12.287060640793573	0.00101623324831875
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2245	0.0	46.997784	1
GTATCAA	3805	0.0	39.845272	1
TCAACGC	4680	0.0	31.736801	4
CAACGCA	4800	0.0	30.968664	5
ATCAACG	4970	0.0	30.078234	3
AACGCAG	5210	0.0	28.893982	6
GTACATG	7810	0.0	28.844835	1
TATCAAC	5325	0.0	27.983465	2
TACATGG	8085	0.0	26.765556	2
ACATGGG	8250	0.0	25.655415	3
CATGGGG	6870	0.0	22.586851	4
ACGCAGA	6570	0.0	22.551483	7
CGCAGAG	6785	0.0	21.27696	8
GAGTACA	5695	0.0	19.11096	1
AGAGTAC	6375	0.0	18.436886	10-11
ATGGGGG	3720	0.0	17.617954	5
TATACCG	245	3.7721147E-6	17.445997	5
GTATATA	965	0.0	17.237787	1
GCAGAGT	7845	0.0	17.191666	9
CAGAGTA	7075	0.0	15.773715	10-11
>>END_MODULE