FastQCFastQC Report
Mon 15 Aug 2016
SRR2921653_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921653_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2259806
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23700.10487625928951422No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19500.048.6807171
GTATCAA33850.042.3457571
TCAACGC40050.034.0111854
CAACGCA41000.032.8729325
ATCAACG41800.032.5879943
TATCAAC43750.031.6800372
AACGCAG44350.030.5889666
GTACATG47800.027.0086351
TACATGG49300.025.7066352
ACGCAGA51600.025.4682677
CGCAGAG53700.023.6787458
ACATGGG52000.023.366233
CATGGGG40400.022.0860924
GAGTACA36200.021.5028951
CGTATAC1353.817732E-421.0945153
GCAGAGT60200.019.9398659
GTATATA6600.019.4169711
GTATAGA5650.019.3215071
TAAGGTG10600.019.2515855
GGTAAGG9150.018.673833
Produced by FastQC (version 0.11.5)