##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921653_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2259806
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.538707747479208	31.0	31.0	34.0	27.0	34.0
2	30.58598702720499	33.0	31.0	34.0	27.0	34.0
3	30.64723166501903	33.0	31.0	34.0	26.0	34.0
4	34.115764362073556	37.0	35.0	37.0	30.0	37.0
5	34.119744349736216	37.0	35.0	37.0	30.0	37.0
6	34.17037303202133	37.0	35.0	37.0	30.0	37.0
7	34.08418466009914	37.0	35.0	37.0	30.0	37.0
8	34.05519987113938	37.0	35.0	37.0	30.0	37.0
9	35.63192238625793	39.0	37.0	39.0	30.0	39.0
10-11	35.537443479661526	39.0	37.0	39.0	30.0	39.0
12-13	35.4335980610725	39.0	37.0	39.0	30.0	39.0
14-15	36.590407981924116	40.0	37.0	41.0	30.0	41.0
16-17	36.53718549291399	40.0	37.0	41.0	29.0	41.0
18-19	36.51412090241375	40.0	37.0	41.0	29.0	41.0
20-21	36.43942134855824	40.0	37.0	41.0	29.0	41.0
22-23	36.3405856520427	40.0	37.0	41.0	28.5	41.0
24-25	36.2269022208101	40.0	36.5	41.0	28.0	41.0
26-27	36.080437878295754	40.0	36.0	41.0	27.0	41.0
28-29	35.94296346677547	40.0	36.0	41.0	27.0	41.0
30-31	35.782617844186625	40.0	36.0	41.0	27.0	41.0
32-33	35.585785239971926	40.0	35.5	41.0	26.0	41.0
34-35	35.399469246475135	39.0	35.0	41.0	25.0	41.0
36-37	35.358437184430876	39.0	35.0	41.0	25.0	41.0
38-39	35.51091244115645	40.0	35.0	41.0	25.5	41.0
40-41	35.55534988401659	40.0	35.0	41.0	25.5	41.0
42-43	35.37092254821874	40.0	35.0	41.0	24.5	41.0
44-45	35.227978419386446	40.0	35.0	41.0	24.0	41.0
46-47	35.048379816674526	40.0	35.0	41.0	23.5	41.0
48-49	34.864954558046136	39.5	35.0	41.0	22.5	41.0
50-51	33.15153269793956	37.0	32.5	39.5	18.5	40.0
52-53	33.2629132766264	37.5	33.0	39.5	19.0	40.0
54-55	34.1729894070553	39.0	34.0	40.5	20.0	41.0
56-57	34.18929058512102	39.0	34.0	41.0	19.5	41.0
58-59	33.9996758571311	38.0	34.0	41.0	18.5	41.0
60-61	33.748624439443034	38.0	34.0	40.5	17.5	41.0
62-63	33.45235741475153	37.0	34.0	40.0	13.5	41.0
64-65	33.15978584002343	37.0	33.5	40.0	12.5	41.0
66-67	32.81730113115905	36.0	33.0	39.5	8.0	41.0
68-69	32.45431289234563	36.0	33.0	39.0	7.0	41.0
70-71	32.04165534563586	35.0	32.5	39.0	2.0	40.5
72-73	31.673185662840083	35.0	32.0	37.5	2.0	40.0
74-75	31.27459348280339	35.0	32.0	37.0	2.0	39.0
76-77	30.889398913004037	35.0	32.0	36.5	2.0	39.0
78-79	30.521784392111535	35.0	31.0	36.0	2.0	39.0
80-81	30.18528935669699	35.0	31.0	36.0	2.0	37.0
82-83	29.851048497083376	35.0	31.0	35.0	2.0	37.0
84-85	29.562790566977874	35.0	31.0	35.0	2.0	36.5
86-87	29.300079520100397	35.0	30.5	35.0	2.0	36.0
88-89	29.038329396417215	35.0	30.0	35.0	2.0	36.0
90-91	28.77948527439966	34.5	30.0	35.0	2.0	35.5
92-93	28.555439050962782	34.0	30.0	35.0	2.0	35.0
94-95	28.290574500642975	34.0	29.5	35.0	2.0	35.0
96-97	28.017279359378637	34.0	29.0	35.0	2.0	35.0
98-99	27.702657440505956	34.0	29.0	35.0	2.0	35.0
100-101	26.591829121614865	33.0	26.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	69007.0
3	12201.0
4	8472.0
5	6399.0
6	5967.0
7	6482.0
8	6843.0
9	7484.0
10	8052.0
11	8703.0
12	8948.0
13	9205.0
14	10046.0
15	11175.0
16	11626.0
17	12235.0
18	11286.0
19	10465.0
20	10477.0
21	10983.0
22	11649.0
23	13096.0
24	15352.0
25	17965.0
26	21129.0
27	24973.0
28	29304.0
29	34813.0
30	40947.0
31	50097.0
32	61735.0
33	79873.0
34	108174.0
35	158747.0
36	264612.0
37	472422.0
38	510022.0
39	98828.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.42596727326983	18.090331229857927	12.255721687756765	25.227979809115475
2	16.443390503013475	19.784561972198976	38.50397885031818	25.268068674469372
3	19.33064101882849	23.150583630019305	29.751979382538924	27.766795968613277
4	13.482637074561833	15.379318590419173	36.334478757981806	34.80356557703719
5	14.082782018509654	36.84024319990932	34.747778172847994	14.329196608733039
6	33.825872372967766	35.20532983062369	16.751756075454598	14.217041720953945
7	28.808736765376903	31.783498096267813	21.01983218477997	18.387932953575312
8	26.486028107101394	34.443392556260896	20.174079380232794	18.896499956404913
9	26.47694785941248	15.234061761503941	19.652646858376922	38.63634352070665
10-11	25.41314806856791	25.01483708416886	28.289373815301428	21.282641031961802
12-13	25.98827170794083	22.787843969189	27.689334371402406	23.534549951467763
14-15	23.045109361965007	24.532641033297203	25.036017943786554	27.38623166095124
16-17	23.23768262330434	27.563786197668495	26.093482416773522	23.105048762253638
18-19	23.173218946420413	26.74666669617064	27.534201561571663	22.545912795837282
20-21	23.574943778500792	26.146659661754818	27.457491412417728	22.820905147326663
22-23	23.505146835371875	26.057128135140644	27.105839971013847	23.331885058473638
24-25	23.29930444551962	26.125213086322148	27.251743091488013	23.32373937667022
26-27	23.121037164867882	26.295222323750867	27.22504665608693	23.35869385529432
28-29	23.238530299234984	26.657268090010366	27.05688093533172	23.047320675422934
30-31	23.448382229999545	26.322878647666514	26.804476621786264	23.424262500547673
32-33	23.178677379932143	26.396750616307063	26.70611562918418	23.71845637457662
34-35	23.790517885919634	26.331611346922028	26.726332496421534	23.1515382707368
36-37	23.72316844949958	26.09566451440464	26.794378954178544	23.38678808191724
38-39	23.39595221284327	26.249281716808014	26.873228354645256	23.481537715703457
40-41	23.651954846076933	26.069577965834306	26.978803039143383	23.29966414894537
42-43	23.535345807345806	26.116878724878724	26.63876923076923	23.709006237006236
44-45	23.35707715748937	26.226044368754277	26.907876607784846	23.509001865971506
46-47	23.60992560936514	26.198388919755043	26.56850194900182	23.623183521877994
48-49	23.271348742973437	26.333248119210495	26.592992295032136	23.80241084278393
50-51	23.360545704745586	26.41188887171935	26.667022866949665	23.56054255658541
52-53	23.685855316763828	26.123456932460332	26.615092324089133	23.575595426686707
54-55	23.06993752897463	26.08551637533697	26.978605719079702	23.865940376608695
56-57	23.17404607232335	26.25146886016451	26.794492605813357	23.77999246169878
58-59	23.165858962851537	25.97396751682519	26.912501906914205	23.947671613409067
60-61	22.982386894057488	26.085335864168652	27.079036124274065	23.853241117499795
62-63	23.15546338285916	26.00174619665686	27.08320862083721	23.75958179964677
64-65	22.946998929566924	25.97695863977843	26.93952806938038	24.136514361274266
66-67	22.8804937158141	26.002200667827086	26.911888372975728	24.20541724338309
68-69	23.15865737197706	25.834908115768012	26.96726610379464	24.039168408460284
70-71	23.005140480379655	25.80149175680999	26.856898505161876	24.336469257648485
72-73	23.027809074829587	25.89369525879595	26.81684374073005	24.26165192564441
74-75	23.03430185034141	26.10371232213734	26.73988426747555	24.122101560045696
76-77	23.14542795906405	25.809048933549583	26.820764680375238	24.22475842701113
78-79	23.064177098992907	25.908222136487204	26.848639252154094	24.1789615123658
80-81	23.242227735978634	26.004179150416817	26.68524806027414	24.068345053330404
82-83	23.215402382656684	25.99445891731129	26.68826952706101	24.101869172971014
84-85	23.200285063838912	25.995023800036677	26.70389171121898	24.100799424905436
86-87	23.2913621996322	26.058556140956295	26.540284570407625	24.10979708900388
88-89	23.127288726420836	25.91093889537051	26.58877780318189	24.37299457502676
90-91	23.168715153181843	25.89427414382726	26.65191105827874	24.285099644712155
92-93	23.40163514519864	25.729674563259753	26.772475484575764	24.096214806965843
94-95	23.34265373224759	25.524616187776466	26.829886012881488	24.302844067094455
96-97	23.4289999254788	25.28854786567731	26.773116654069035	24.509335554774857
98-99	23.297821874665257	25.429086011477775	26.713987679474727	24.55910443438224
100-101	23.561654773192274	25.26448980044469	26.838753319915053	24.33510210644798
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	134.0
1	115.0
2	147.0
3	206.0
4	184.0
5	206.5
6	319.0
7	557.0
8	853.5
9	918.5
10	792.5
11	712.0
12	726.5
13	779.5
14	854.0
15	979.0
16	1063.5
17	1140.0
18	1279.5
19	1404.0
20	1582.0
21	1808.5
22	2007.0
23	2282.5
24	2650.0
25	3159.5
26	4109.0
27	5528.0
28	7432.0
29	10249.0
30	14283.5
31	19483.0
32	27087.5
33	31878.0
34	33881.0
35	38068.5
36	43735.5
37	49668.5
38	54735.0
39	59545.0
40	63983.5
41	68258.0
42	72547.5
43	76831.5
44	80485.5
45	85680.5
46	89561.5
47	91561.5
48	93887.5
49	94587.5
50	94582.5
51	93854.0
52	92390.5
53	90966.5
54	88632.0
55	84016.0
56	77020.0
57	68835.5
58	62002.5
59	54356.5
60	44816.5
61	37053.5
62	31295.5
63	24481.0
64	17433.0
65	12570.5
66	9515.0
67	7555.5
68	5901.5
69	4573.0
70	3623.5
71	2699.5
72	1891.5
73	1445.0
74	1159.0
75	886.0
76	664.0
77	466.0
78	290.0
79	197.0
80	146.0
81	103.5
82	90.5
83	83.0
84	68.0
85	47.0
86	37.5
87	35.5
88	27.0
89	22.0
90	20.5
91	13.5
92	9.5
93	13.0
94	12.5
95	8.0
96	9.0
97	6.5
98	6.5
99	7.5
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.051464594748398755
2	0.07151056329614135
3	0.06752792053831169
4	0.06841295226227384
5	0.062704497642718
6	0.009823852135979813
7	0.013142721100837858
8	0.016682847996686442
9	0.0064607315849236615
10-11	0.011726670342498427
12-13	0.02137351613368581
14-15	0.038166993095867525
16-17	0.025621668408704112
18-19	0.009890229515276974
20-21	0.019426446340969093
22-23	0.035246388406792444
24-25	0.032967431717589916
26-27	0.020112345927039754
28-29	0.010797387032338174
30-31	0.01084163861853628
32-33	0.00785465655016404
34-35	0.004845548688692746
36-37	5.752706205753946E-4
38-39	0.0034294979303533136
40-41	0.0010841638618536281
42-43	0.22650174395501205
44-45	0.19526012409914834
46-47	0.20130046561519
48-49	0.20809308409659943
50-51	0.20003929540854393
52-53	0.20707529761404297
54-55	0.2035794223043925
56-57	0.20603538533838744
58-59	0.21479719940561268
60-61	0.2190896032668291
62-63	0.2043980766490575
64-65	0.2055043663040102
66-67	0.20273864216662849
68-69	0.19771608713314326
70-71	0.18928615996240386
72-73	0.2006809434084165
74-75	0.19999504382234584
76-77	0.19382194754770984
78-79	0.21223060740612246
80-81	0.22309437181775782
82-83	0.22178895002491367
84-85	0.21561585375027767
86-87	0.2354184385739307
88-89	0.22747527885137042
90-91	0.2343564005051761
92-93	0.23032950616114836
94-95	0.2324535822986575
96-97	0.23957808767655275
98-99	0.2334492429881149
100-101	0.23977721981444425
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2259806.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.37983262261686
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.14342861807587	49.36953830375598
2	11.95594198707842	14.91619319999338
3	3.85010936583177	7.20507533458066
4	1.7113949654122071	4.270261259744106
5	0.9004597734873561	2.8085264976770383
6	0.5539968301665966	2.073493772355156
7	0.36881019864310416	1.6104422922609642
8	0.26366681290804445	1.3157993325874118
9	0.205463238852694	1.153508620475862
>10	0.9951170992820338	10.54847359515042
>50	0.03508215690288739	1.4721833002632079
>100	0.014109372815112951	1.6889761972600583
>500	0.0014232826728911665	0.6153184469909729
>1k	9.96297871022798E-4	0.9522098469047194
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2370	0.10487625928951422	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.4251586198107274E-5	0.0
3	0.0	0.0	0.0	4.4251586198107274E-5	0.0
4	0.0	0.0	0.0	8.850317239621455E-5	0.0
5	0.0	0.0	0.0	8.850317239621455E-5	4.4251586198107274E-5
6	0.0	0.0	0.0	8.850317239621455E-5	4.4251586198107274E-5
7	0.0	0.0	0.0	8.850317239621455E-5	4.4251586198107274E-5
8	0.0	0.0	0.0	8.850317239621455E-5	8.850317239621455E-5
9	0.0	0.0	0.0	1.327547585943218E-4	8.850317239621455E-5
10-11	0.0	0.0	0.0	1.327547585943218E-4	8.850317239621455E-5
12-13	0.0	0.0	0.0	1.327547585943218E-4	8.850317239621455E-5
14-15	0.0	0.0	0.0	1.327547585943218E-4	1.327547585943218E-4
16-17	0.0	0.0	0.0	1.327547585943218E-4	1.327547585943218E-4
18-19	0.0	0.0	0.0	1.327547585943218E-4	1.327547585943218E-4
20-21	0.0	0.0	0.0	1.327547585943218E-4	1.327547585943218E-4
22-23	0.0	0.0	0.0	2.2125793099053636E-4	1.327547585943218E-4
24-25	0.0	0.0	0.0	6.858995860706627E-4	1.770063447924291E-4
26-27	0.0	0.0	0.0	0.002013447172013881	1.770063447924291E-4
28-29	0.0	0.0	0.0	0.007279385929588647	1.770063447924291E-4
30-31	0.0	0.0	0.0	0.029515807994137548	1.770063447924291E-4
32-33	0.0	0.0	0.0	0.08109103170803159	1.770063447924291E-4
34-35	0.0	0.0	0.0	0.15361938148672938	2.2125793099053636E-4
36-37	0.0	0.0	0.0	0.2665273036712001	2.2125793099053636E-4
38-39	0.0	0.0	0.0	0.4287757444665604	2.2125793099053636E-4
40-41	0.0	0.0	0.0	0.6065564920174564	2.655095171886436E-4
42-43	0.0	0.0	0.0	0.7952895071523839	3.097611033867509E-4
44-45	0.0	0.0	0.0	1.0363500229665732	3.540126895848582E-4
46-47	0.0	0.0	0.0	1.3209983511858983	3.540126895848582E-4
48-49	0.0	0.0	0.0	1.5984115450618326	3.9826427578296543E-4
50-51	0.0	0.0	0.0	1.8827722379708702	4.425158619810727E-4
52-53	0.0	0.0	0.0	2.1705624288102605	4.8676744817918E-4
54-55	0.0	0.0	0.0	2.5112996425356866	5.310190343772872E-4
56-57	0.0	0.0	0.0	2.910338321077119	5.310190343772872E-4
58-59	0.0	0.0	0.0	3.3150633284450084	5.310190343772872E-4
60-61	0.0	0.0	0.0	3.7278199987078535	5.310190343772872E-4
62-63	0.0	0.0	0.0	4.151285552830641	5.752706205753946E-4
64-65	0.0	0.0	0.0	4.620927637151153	5.752706205753946E-4
66-67	0.0	0.0	0.0	5.173497194006919	5.752706205753946E-4
68-69	0.0	0.0	0.0	5.758724421476888	5.752706205753946E-4
70-71	0.0	0.0	0.0	6.343310000946984	6.63773792971609E-4
72-73	0.0	0.0	0.0	6.955774079721888	7.080253791697164E-4
74-75	0.0	0.0	0.0	7.6008737033178955	7.080253791697164E-4
76-77	0.0	0.0	0.0	8.334742008827307	7.080253791697164E-4
78-79	0.0	0.0	0.0	9.116313524258278	7.080253791697164E-4
80-81	0.0	0.0	0.0	9.924081978718528	7.301511722687701E-4
82-83	0.0	0.0	0.0	10.747869507382493	7.965285515659309E-4
84-85	0.0	0.0	0.0	11.625776725966741	7.965285515659309E-4
86-87	0.0	0.0	0.0	12.554064375437537	7.965285515659309E-4
88-89	0.0	0.0	0.0	13.537666507655967	7.965285515659309E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1950	0.0	48.680717	1
GTATCAA	3385	0.0	42.345757	1
TCAACGC	4005	0.0	34.011185	4
CAACGCA	4100	0.0	32.872932	5
ATCAACG	4180	0.0	32.587994	3
TATCAAC	4375	0.0	31.680037	2
AACGCAG	4435	0.0	30.588966	6
GTACATG	4780	0.0	27.008635	1
TACATGG	4930	0.0	25.706635	2
ACGCAGA	5160	0.0	25.468267	7
CGCAGAG	5370	0.0	23.678745	8
ACATGGG	5200	0.0	23.36623	3
CATGGGG	4040	0.0	22.086092	4
GAGTACA	3620	0.0	21.502895	1
CGTATAC	135	3.817732E-4	21.094515	3
GCAGAGT	6020	0.0	19.939865	9
GTATATA	660	0.0	19.416971	1
GTATAGA	565	0.0	19.321507	1
TAAGGTG	1060	0.0	19.251585	5
GGTAAGG	915	0.0	18.67383	3
>>END_MODULE