FastQCFastQC Report
Mon 15 Aug 2016
SRR2921653_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921653_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2259806
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17600.053.4814031
GTATCAA33350.042.3361051
TCAACGC42900.032.3038564
CAACGCA43150.032.032945
ATCAACG44150.031.2817543
TATCAAC44950.030.6194322
AACGCAG46500.029.8246976
GTACATG49200.024.7357561
ACGCAGA56750.024.3541647
CGCAGAG59450.023.0883088
TACATGG53400.022.3969122
ACATGGG53550.021.625153
TAAGGTG10600.020.1646755
GCAGAGT65450.020.0292539
CATGGGG43150.018.4780984
AGAGTAC56800.018.31306810-11
GAGTACT35400.018.02937112-13
GAGTACA40700.017.1701151
CAGAGTA63600.016.72847210-11
TCTATAC4559.094947E-1216.6892743
Produced by FastQC (version 0.11.5)