##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921653_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2259806
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.31177145294773	33.0	31.0	34.0	28.0	34.0
2	31.4435363035588	34.0	31.0	34.0	28.0	34.0
3	31.513820655401393	34.0	31.0	34.0	28.0	34.0
4	34.996519170229654	37.0	35.0	37.0	32.0	37.0
5	34.734067437647305	37.0	35.0	37.0	32.0	37.0
6	34.7040064501112	37.0	35.0	37.0	32.0	37.0
7	34.589419180230514	37.0	35.0	37.0	32.0	37.0
8	34.529799903177526	37.0	35.0	37.0	31.0	37.0
9	36.112502577654894	39.0	37.0	39.0	32.0	39.0
10-11	36.00528408190792	39.0	37.0	39.0	31.0	39.0
12-13	35.92532434200104	39.0	37.0	39.0	31.0	39.0
14-15	37.15579213436906	40.0	38.0	41.0	31.0	41.0
16-17	37.1209765351539	40.0	38.0	41.0	31.0	41.0
18-19	37.08644680118559	40.0	38.0	41.0	31.0	41.0
20-21	37.02612348139619	40.0	38.0	41.0	31.0	41.0
22-23	36.92981742680567	40.0	37.0	41.0	30.0	41.0
24-25	36.83787103848738	40.0	37.0	41.0	30.0	41.0
26-27	36.70549529472884	40.0	37.0	41.0	30.0	41.0
28-29	36.576964128779196	40.0	37.0	41.0	30.0	41.0
30-31	36.40237967329939	40.0	36.5	41.0	29.5	41.0
32-33	36.32533788298642	40.0	36.5	41.0	29.5	41.0
34-35	36.42839318950388	40.0	37.0	41.0	30.0	41.0
36-37	36.40602157884349	40.0	37.0	41.0	30.0	41.0
38-39	36.279721577869964	40.0	36.5	41.0	29.0	41.0
40-41	36.17347595324554	40.0	36.0	41.0	28.5	41.0
42-43	36.02229306409488	40.0	36.0	41.0	28.0	41.0
44-45	35.86611483463625	40.0	36.0	41.0	27.0	41.0
46-47	35.68618744263888	40.0	35.0	41.0	26.5	41.0
48-49	35.51893038605969	40.0	35.0	41.0	26.0	41.0
50-51	35.34874121937901	39.5	35.0	41.0	26.0	41.0
52-53	35.160875092817705	39.0	35.0	41.0	25.5	41.0
54-55	34.941544318406095	39.0	35.0	41.0	24.0	41.0
56-57	34.70954011096528	39.0	35.0	41.0	24.0	41.0
58-59	34.46276760040463	38.0	34.5	40.0	23.0	41.0
60-61	34.186602080001556	38.0	34.0	40.0	23.0	41.0
62-63	33.882143422930994	37.0	34.0	40.0	22.0	41.0
64-65	33.54262777424257	37.0	34.0	40.0	22.0	41.0
66-67	33.1890591493252	36.0	33.5	39.0	20.5	41.0
68-69	32.828178392304466	36.0	33.0	39.0	19.5	41.0
70-71	32.43173285671425	35.0	33.0	38.5	18.0	40.0
72-73	32.073711415935705	35.0	33.0	37.5	16.0	40.0
74-75	31.675298012307252	35.0	32.5	37.0	12.0	39.0
76-77	28.97677145737289	33.0	28.0	34.5	7.0	36.5
78-79	30.536004196820436	34.5	31.0	36.0	7.0	37.5
80-81	30.716055050743293	35.0	32.0	35.5	6.5	37.0
82-83	30.54200249932959	35.0	32.0	35.0	2.0	37.0
84-85	30.333078370444188	35.0	32.0	35.0	2.0	36.5
86-87	30.117670720406974	35.0	31.5	35.0	2.0	36.0
88-89	29.84079695336679	35.0	31.0	35.0	2.0	36.0
90-91	29.635337723680706	34.0	31.0	35.0	2.0	35.0
92-93	29.470294352701075	34.0	31.0	35.0	2.0	35.0
94-95	29.358496039040517	34.0	31.0	35.0	2.0	35.0
96-97	29.148395702993973	34.0	31.0	35.0	2.0	35.0
98-99	28.93666292593258	34.0	31.0	35.0	2.0	35.0
100-101	27.81283680988545	33.0	28.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	44686.0
3	15218.0
4	8533.0
5	5999.0
6	5421.0
7	5295.0
8	5541.0
9	5996.0
10	6642.0
11	7272.0
12	7882.0
13	8686.0
14	9425.0
15	10482.0
16	11363.0
17	12349.0
18	11154.0
19	9975.0
20	9527.0
21	9293.0
22	9580.0
23	10615.0
24	12443.0
25	14973.0
26	17745.0
27	21024.0
28	25278.0
29	30525.0
30	36465.0
31	45163.0
32	57294.0
33	74773.0
34	105137.0
35	158254.0
36	277018.0
37	524385.0
38	554353.0
39	74041.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.465164901748636	19.526253478900102	12.762194600330393	26.246387019020865
2	15.554320442976666	21.160871102481057	36.97357690028733	26.31123155425495
3	18.057036896074067	24.20204550383034	29.484596763129595	28.256320836965998
4	13.050393511224717	16.623105463840517	35.21120028889353	35.11530073604124
5	13.808225871988286	37.261858075778726	34.75298570949275	14.17693034274024
6	33.86378742540144	35.73460390961526	16.4523520618543	13.949256603129001
7	28.695427837610836	32.026687246604354	20.920114381500003	18.3577705342848
8	26.495283223427148	34.91799738561629	19.83922513702504	18.74749425393153
9	26.341110697112942	15.262504834485794	19.508444530194186	38.88793993820708
10-11	25.384701375660907	25.29226015032767	28.177307405812773	21.145731068198646
12-13	25.955804033898588	22.913232548558522	27.65606587134519	23.474897546197692
14-15	22.972324417472322	24.724194522324762	24.97821059835611	27.325270461846806
16-17	23.131830785474506	27.834491102333565	26.008648530006557	23.025029582185375
18-19	23.12093695991617	26.94779771362345	27.448289687055333	22.482975639405048
20-21	23.49967331172522	26.279189813078553	27.43515519850323	22.785981676692998
22-23	23.411368055487948	26.132884858257743	27.128788931439246	23.32695815481506
24-25	23.267978570617814	26.287921313199053	27.199287368829335	23.244812747353798
26-27	23.02234235382415	26.51703024767407	27.210847777447356	23.24977962105442
28-29	23.19452084377908	26.746687932111072	27.03679396873289	23.021997255376952
30-31	23.436645949262942	26.43018022503677	26.75758247682072	23.375591348879574
32-33	23.10258679825629	26.54468810161315	26.656878582018546	23.695846518112013
34-35	23.656099329590322	26.518257638503652	26.753986400621177	23.071656631284856
36-37	23.6781070524854	26.196346086274637	26.798081527131313	23.327465334108652
38-39	23.383494729966806	26.39368742132896	26.882490550624823	23.340327298079412
40-41	23.585596174415297	26.237104118052883	26.904417468789454	23.272882238742366
42-43	23.509318598118888	26.296467416438418	26.51400389711372	23.68021008832897
44-45	23.355925227689124	26.30690015312038	26.84976943433968	23.487405184850818
46-47	23.546286503254812	26.326065503400848	26.49949330229718	23.62815469104716
48-49	23.223986703950246	26.49298985647356	26.517883439173474	23.765140000402724
50-51	23.34881290123301	26.492121041547463	26.54484804312684	23.614218014092692
52-53	23.615657125181464	26.268102834655043	26.511623893385305	23.60461614677819
54-55	22.966757769560424	26.255401121202844	26.790733879832686	23.987107229404046
56-57	23.092524591832557	26.37713709137618	26.595490215819538	23.934848100971724
58-59	23.037320502587225	26.17392099071087	26.687426711193364	24.101331795508546
60-61	22.86401688407181	26.306416783702037	26.84714343340291	23.982422898823245
62-63	22.994459033183393	26.23569667953613	26.789837621655405	23.98000666562507
64-65	22.724908351775127	26.124691167284738	26.69633880780501	24.454061673135126
66-67	22.728471261474457	26.20642430030811	26.6188770525502	24.446227385667232
68-69	22.984690833737144	26.004309154973072	26.61867254738886	24.39232746390092
70-71	22.784868421780953	25.97465080930762	26.51409327767095	24.72638749124048
72-73	22.766518876059514	26.059293898502556	26.473250726410903	24.700936499027026
74-75	22.730420445177245	26.22661657546601	26.35666188992846	24.686301089428287
76-77	22.757062683135448	25.94263542958673	26.586948436996526	24.713353450281296
78-79	22.654522593870137	25.980199265128732	26.57213091629939	24.793147224701737
80-81	22.823090505526867	26.022594554502028	26.3938050403237	24.76050989964741
82-83	22.65208043085905	25.99882923547306	26.43816471041283	24.910925623255057
84-85	22.575141556611356	25.96251633175229	26.45850129967764	25.003840811958717
86-87	22.617416587107734	26.011985206466505	26.3705096436055	25.000088562820267
88-89	22.389642563981138	25.873218517498547	26.379866005043173	25.357272913477143
90-91	22.277688822607114	25.79753951107575	26.476099169665435	25.448672496651696
92-93	22.411062214997628	25.65183482917955	26.60446413964842	25.332638816174395
94-95	22.211165604965874	25.448578542402977	26.734745629943944	25.605510222687204
96-97	22.164500171941683	25.184389199849345	26.767403397319832	25.883707230889136
98-99	21.992970137021704	25.349612441521064	26.658429905814174	25.998987515643062
100-101	21.92973799882202	25.25682593894413	26.67128802163378	26.142148040600073
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	147.0
1	111.5
2	114.0
3	171.5
4	172.5
5	175.0
6	286.0
7	519.5
8	779.5
9	842.0
10	672.0
11	541.0
12	537.0
13	565.5
14	601.5
15	678.0
16	724.5
17	759.5
18	875.5
19	1018.0
20	1114.5
21	1251.5
22	1496.0
23	1755.5
24	2166.0
25	2780.0
26	3729.5
27	5179.0
28	7275.0
29	10316.0
30	14425.0
31	19728.5
32	27639.0
33	32361.0
34	33940.5
35	38187.5
36	44029.5
37	49801.0
38	54600.5
39	59178.5
40	63739.5
41	68389.5
42	72657.0
43	76780.5
44	80602.5
45	85944.5
46	89956.5
47	92249.5
48	94552.0
49	95164.5
50	95389.0
51	94691.0
52	93489.5
53	92494.0
54	90229.5
55	85083.5
56	77557.0
57	69115.5
58	61978.5
59	54362.5
60	44937.0
61	37089.0
62	31016.5
63	23985.5
64	16949.0
65	12216.0
66	9154.0
67	7115.0
68	5572.0
69	4283.5
70	3277.5
71	2436.5
72	1737.5
73	1294.5
74	1026.0
75	774.0
76	537.5
77	323.5
78	177.0
79	116.5
80	69.0
81	39.5
82	21.0
83	12.5
84	10.5
85	10.5
86	9.0
87	5.5
88	2.5
89	2.0
90	1.0
91	1.0
92	1.0
93	1.0
94	1.5
95	1.0
96	0.0
97	0.0
98	1.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16395212686398744
2	1.327547585943218E-4
3	0.00955834261879117
4	0.00663773792971609
5	0.024072862891770356
6	0.0019470697927167198
7	0.0
8	0.0
9	0.0
10-11	0.04750407778366816
12-13	3.097611033867509E-4
14-15	0.004712793930098424
16-17	0.0
18-19	0.0013054217928441646
20-21	2.876353102876973E-4
22-23	0.0
24-25	5.088932412782337E-4
26-27	0.002743598344282651
28-29	0.005088932412782336
30-31	0.015620809927931868
32-33	0.011328406066715462
34-35	0.009624719998088331
36-37	0.004071145930225869
38-39	2.2125793099053637E-5
40-41	0.0032303657924618308
42-43	0.008186543446649845
44-45	0.0064607315849236615
46-47	0.003363120551056153
48-49	0.008297172412145113
50-51	0.0023453340684996852
52-53	0.001880692413419559
54-55	0.003982642757829654
56-57	0.007235134343390539
58-59	0.014934910341861203
60-61	0.007146631170994324
62-63	0.02004596854774259
64-65	0.03969367281970222
66-67	0.060425540953515475
68-69	0.04984941185216784
70-71	0.06852358122776911
72-73	0.033365695993372885
74-75	0.026263316408576664
76-77	0.22818330423054015
78-79	0.08228582453538047
80-81	0.06719603364182589
82-83	0.0264845743395672
84-85	0.0517301042655874
86-87	0.06735091419351927
88-89	0.2947819414586916
90-91	0.3008444087678323
92-93	0.051442468955299706
94-95	0.009912355308376029
96-97	0.01411625599719622
98-99	7.965285515659309E-4
100-101	3.097611033867509E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2259806.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.01971514901143
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.85729105972982	48.90706728947129
2	12.120759373491106	15.034520874672571
3	3.8581800819979355	7.178496890373047
4	1.718618345387438	4.26352880923177
5	0.9339177138182015	2.8960655291810413
6	0.5989601134706047	2.228840137383988
7	0.3704639248697119	1.6083246965383016
8	0.2787880942955607	1.3832286556117128
9	0.1969265160069077	1.099199378523209
>10	1.012403748959396	10.64625571868571
>50	0.03736289741215881	1.5572827289396083
>100	0.014110697254824172	1.7216836147057288
>500	0.0011444817064216338	0.4812916742733352
>1k	0.0010729515997692145	0.9942140024086511
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	4.4251586198107274E-5
7	0.0	0.0	0.0	0.0	4.4251586198107274E-5
8	0.0	0.0	0.0	0.0	4.4251586198107274E-5
9	0.0	0.0	0.0	0.0	8.850317239621455E-5
10-11	0.0	0.0	0.0	4.4251586198107274E-5	8.850317239621455E-5
12-13	0.0	0.0	0.0	4.4251586198107274E-5	2.2125793099053636E-4
14-15	0.0	0.0	0.0	4.4251586198107274E-5	2.655095171886436E-4
16-17	0.0	0.0	0.0	4.4251586198107274E-5	3.9826427578296543E-4
18-19	0.0	0.0	0.0	4.4251586198107274E-5	3.9826427578296543E-4
20-21	0.0	0.0	0.0	4.4251586198107274E-5	4.425158619810727E-4
22-23	0.0	0.0	0.0	1.327547585943218E-4	4.425158619810727E-4
24-25	0.0	0.0	0.0	5.752706205753946E-4	4.425158619810727E-4
26-27	0.0	0.0	0.0	0.001880692413419559	4.425158619810727E-4
28-29	0.0	0.0	0.0	0.007235134343390539	4.425158619810727E-4
30-31	0.0	0.0	0.0	0.029471556407939442	4.425158619810727E-4
32-33	0.0	0.0	0.0	0.08175480550100318	4.425158619810727E-4
34-35	0.0	0.0	0.0	0.15410614893490857	4.8676744817918E-4
36-37	0.0	0.0	0.0	0.26679281318838877	4.8676744817918E-4
38-39	0.0	0.0	0.0	0.4283996059838765	4.8676744817918E-4
40-41	0.0	0.0	0.0	0.6053174476039094	4.8676744817918E-4
42-43	0.0	0.0	0.0	0.7936079468768558	4.8676744817918E-4
44-45	0.0	0.0	0.0	1.0346905884841442	4.8676744817918E-4
46-47	0.0	0.0	0.0	1.31885214925529	4.8676744817918E-4
48-49	0.0	0.0	0.0	1.5955573177520548	4.8676744817918E-4
50-51	0.0	0.0	0.0	1.8786568404544461	4.8676744817918E-4
52-53	0.0	0.0	0.0	2.1656283769491718	4.8676744817918E-4
54-55	0.0	2.2125793099053637E-5	0.0	2.5038653760544047	4.8676744817918E-4
56-57	0.0	4.4251586198107274E-5	0.0	2.902395161354559	4.8676744817918E-4
58-59	0.0	4.4251586198107274E-5	0.0	3.3061245080329904	5.088932412782336E-4
60-61	0.0	4.4251586198107274E-5	0.0	3.7172438696065058	5.310190343772872E-4
62-63	0.0	4.4251586198107274E-5	0.0	4.14006777572942	5.310190343772872E-4
64-65	0.0	4.4251586198107274E-5	0.0	4.609134589429358	5.310190343772872E-4
66-67	0.0	4.4251586198107274E-5	0.0	5.160022586009596	5.310190343772872E-4
68-69	0.0	4.4251586198107274E-5	0.0	5.743678882169531	5.310190343772872E-4
70-71	0.0	4.4251586198107274E-5	0.0	6.327335178329467	5.310190343772872E-4
72-73	0.0	4.4251586198107274E-5	0.0	6.940241772966352	6.416479998725554E-4
74-75	0.0	4.4251586198107274E-5	0.0	7.587133585803383	6.63773792971609E-4
76-77	0.0	4.4251586198107274E-5	0.0	8.323502105932988	6.63773792971609E-4
78-79	0.0	4.4251586198107274E-5	0.0	9.107728716535846	7.080253791697164E-4
80-81	0.0	4.4251586198107274E-5	0.0	9.918948794719547	7.301511722687701E-4
82-83	0.0	4.4251586198107274E-5	0.0	10.746785343520639	7.965285515659309E-4
84-85	0.0	4.4251586198107274E-5	0.0	11.628498198517926	7.965285515659309E-4
86-87	0.0	4.4251586198107274E-5	0.0	12.562361547849683	8.186543446649845E-4
88-89	0.0	4.4251586198107274E-5	0.0	13.553309443376998	8.407801377640382E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1760	0.0	53.481403	1
GTATCAA	3335	0.0	42.336105	1
TCAACGC	4290	0.0	32.303856	4
CAACGCA	4315	0.0	32.03294	5
ATCAACG	4415	0.0	31.281754	3
TATCAAC	4495	0.0	30.619432	2
AACGCAG	4650	0.0	29.824697	6
GTACATG	4920	0.0	24.735756	1
ACGCAGA	5675	0.0	24.354164	7
CGCAGAG	5945	0.0	23.088308	8
TACATGG	5340	0.0	22.396912	2
ACATGGG	5355	0.0	21.62515	3
TAAGGTG	1060	0.0	20.164675	5
GCAGAGT	6545	0.0	20.029253	9
CATGGGG	4315	0.0	18.478098	4
AGAGTAC	5680	0.0	18.313068	10-11
GAGTACT	3540	0.0	18.029371	12-13
GAGTACA	4070	0.0	17.170115	1
CAGAGTA	6360	0.0	16.728472	10-11
TCTATAC	455	9.094947E-12	16.689274	3
>>END_MODULE