FastQCFastQC Report
Mon 15 Aug 2016
SRR2921652_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921652_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3146842
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36600.042.142151
GGTATCA21150.041.736591
TCAACGC44250.034.751524
CAACGCA44450.034.3805165
ATCAACG46900.032.685193
TATCAAC46700.032.317042
AACGCAG48150.031.7229676
GTACATG70800.028.3544711
ACGCAGA58100.025.884857
TACATGG76650.025.6335952
ACATGGG79800.024.5027963
CGCAGAG61450.024.1660528
CATGGGG67750.022.6274324
GAGTACA53650.019.9918581
GCAGAGT71700.019.3897919
TATACCG1507.7689614E-418.9840225
ATGGGGG40000.018.1534715
AGAGTAC58450.018.10305810-11
AGTACAT57350.017.2127882
TAAGGCG9950.017.1714765
Produced by FastQC (version 0.11.5)