##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921652_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3146842
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.456466832462514	31.0	31.0	34.0	26.0	34.0
2	30.50264074268743	31.0	31.0	34.0	26.0	34.0
3	30.565087474998744	33.0	31.0	34.0	26.0	34.0
4	34.029197525646346	37.0	35.0	37.0	30.0	37.0
5	34.02473114315876	37.0	35.0	37.0	30.0	37.0
6	34.07150247772211	37.0	35.0	37.0	30.0	37.0
7	33.9813028426594	37.0	35.0	37.0	30.0	37.0
8	33.954961196018104	37.0	35.0	37.0	30.0	37.0
9	35.52180567057386	39.0	37.0	39.0	30.0	39.0
10-11	35.42171246602149	39.0	36.0	39.0	30.0	39.0
12-13	35.313172062658374	39.0	35.0	39.0	29.0	39.0
14-15	36.46318960405384	40.0	37.0	41.0	29.0	41.0
16-17	36.39911044151565	40.0	37.0	41.0	29.0	41.0
18-19	36.362556175365654	40.0	37.0	41.0	28.0	41.0
20-21	36.2817246623758	40.0	37.0	41.0	27.5	41.0
22-23	36.17979072352536	40.0	36.5	41.0	27.0	41.0
24-25	36.07227515712578	40.0	36.0	41.0	27.0	41.0
26-27	35.91994068974547	40.0	36.0	41.0	27.0	41.0
28-29	35.782886144267806	40.0	36.0	41.0	27.0	41.0
30-31	35.63303448345992	40.0	36.0	41.0	26.0	41.0
32-33	35.45491893142395	39.5	35.0	41.0	25.0	41.0
34-35	35.28017707911614	39.0	35.0	41.0	25.0	41.0
36-37	35.24767846622105	39.0	35.0	41.0	25.0	41.0
38-39	35.42909653551084	40.0	35.0	41.0	25.0	41.0
40-41	35.48811904124834	40.0	35.0	41.0	25.0	41.0
42-43	35.32311155119958	40.0	35.0	41.0	24.0	41.0
44-45	35.20130213083466	40.0	35.0	41.0	24.0	41.0
46-47	35.04207202013956	40.0	35.0	41.0	23.5	41.0
48-49	34.87294389105014	39.0	35.0	41.0	23.0	41.0
50-51	33.15241121098549	37.0	32.5	39.5	18.5	40.0
52-53	33.26944679777377	37.5	33.0	39.5	19.0	40.0
54-55	34.18456503377037	39.0	34.0	40.5	21.0	41.0
56-57	34.200417752146436	39.0	34.0	41.0	20.0	41.0
58-59	34.00537491237247	38.0	34.0	41.0	19.5	41.0
60-61	33.75078062387625	38.0	34.0	40.0	18.5	41.0
62-63	33.44891688238558	37.0	34.0	40.0	17.5	41.0
64-65	33.15352264270021	37.0	33.0	40.0	14.0	41.0
66-67	32.808230600710175	36.0	33.0	39.0	12.5	41.0
68-69	32.439176164548456	36.0	33.0	39.0	8.0	41.0
70-71	32.02285783652309	35.0	32.0	38.5	7.0	40.5
72-73	31.6519528149173	35.0	32.0	37.5	2.0	40.0
74-75	31.247345116151365	35.0	32.0	37.0	2.0	39.0
76-77	30.86313342074372	35.0	32.0	36.5	2.0	39.0
78-79	30.502983149455865	35.0	31.0	36.0	2.0	38.0
80-81	30.173567023701857	35.0	31.0	35.5	2.0	37.0
82-83	29.846716168145715	35.0	31.0	35.0	2.0	37.0
84-85	29.568890017357084	35.0	31.0	35.0	2.0	36.0
86-87	29.31140632418151	35.0	30.5	35.0	2.0	36.0
88-89	29.061441597639792	35.0	30.0	35.0	2.0	36.0
90-91	28.813355421085646	34.0	30.0	35.0	2.0	35.0
92-93	28.59129787895293	34.0	30.0	35.0	2.0	35.0
94-95	28.329453146996258	34.0	29.5	35.0	2.0	35.0
96-97	28.050230199037635	34.0	29.0	35.0	2.0	35.0
98-99	27.734018104499686	34.0	29.0	35.0	2.0	35.0
100-101	26.61532641295623	33.0	26.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	101541.0
3	17908.0
4	12673.0
5	9595.0
6	8937.0
7	8953.0
8	9654.0
9	10372.0
10	11326.0
11	12057.0
12	12030.0
13	12191.0
14	12862.0
15	13707.0
16	13689.0
17	14135.0
18	13906.0
19	13385.0
20	13780.0
21	14582.0
22	15737.0
23	18011.0
24	21173.0
25	25163.0
26	29933.0
27	35098.0
28	41356.0
29	48985.0
30	58524.0
31	71567.0
32	88302.0
33	113029.0
34	156707.0
35	225663.0
36	377688.0
37	672756.0
38	694709.0
39	115150.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.8551039128064	17.493629150590507	11.942127410629263	25.70913952597383
2	16.692277114372487	19.49681194447536	38.11639185257032	25.69451908858183
3	19.56781808329581	22.815621971044138	29.106214749631754	28.510345196028297
4	13.695133003672904	14.946687230693401	35.59327747126071	35.76490229437298
5	14.576539695935473	36.5317526463586	34.265275965699495	14.626431692006436
6	34.708778901550396	34.25447093700263	16.60752241189063	14.42922774955634
7	29.587184246322828	31.13849302704008	20.67158439379108	18.602738332846013
8	27.02016100709368	34.10936137793951	19.891613093514614	18.9788645214522
9	26.919967381100236	15.00866638149494	18.99682264458429	39.07454359282054
10-11	25.92321210610128	24.605296800692464	27.87034516557011	21.601145927636146
12-13	26.348207547034875	22.12089730637403	27.443911994646154	24.086983151944942
14-15	23.546345562001513	23.870428952318463	24.50392463729968	28.079300848380345
16-17	23.974712167981185	26.824472452327846	25.43954756789454	23.761267811796433
18-19	23.938547885517785	26.289418610748715	26.584252736757968	23.18778076697553
20-21	24.362499129911313	25.597166895882722	26.493827058470913	23.546506915735048
22-23	24.291150481440855	25.354851927869465	26.208585111136156	24.145412479553528
24-25	24.053191895173853	25.41350634818709	26.307815549720576	24.225486206918475
26-27	23.86872817707813	25.661491489189594	26.286546217831674	24.183234115900603
28-29	24.025672309862728	25.925908858183195	26.086086680991567	23.962332150962514
30-31	24.25016351574423	25.66934987157102	25.90806061110712	24.172426001577634
32-33	23.99508037688508	25.758072448442082	25.85087154079068	24.395975633882156
34-35	24.373357689228126	25.685092962516105	25.922295987998744	24.01925336025702
36-37	24.40527574622662	25.518607620942507	25.951807470123367	24.124309162707505
38-39	24.156079156935604	25.646635487021207	26.054219786945797	24.143065569097384
40-41	24.351508499260994	25.45246715475993	26.124863869775133	24.071160476203946
42-43	24.24368467266545	25.430775438785346	25.87687798918487	24.448661899364335
44-45	24.030987266196167	25.535075112058365	26.085815152570675	24.348122469174797
46-47	24.198763669432182	25.460981142360783	25.94213369233238	24.39812149587465
48-49	23.96080105351667	25.573795444728187	25.989912874069827	24.475490627685318
50-51	24.00960126140337	25.66122900858395	26.061324021398264	24.267845708614423
52-53	24.228383808502706	25.426547475487908	26.033892616487957	24.31117609952143
54-55	23.741136967730483	25.343237268558138	26.303465767022953	24.61215999668843
56-57	23.802604809992037	25.474227818499497	26.224270567544927	24.498896803963547
58-59	23.899085059485227	25.29699620989383	26.209452766500448	24.5944659641205
60-61	23.815681593047486	25.331360749823766	26.34176044320391	24.511197213924834
62-63	23.876118897785975	25.314943994212346	26.327082588356742	24.481854519644934
64-65	23.721463629919892	25.214824806254864	26.211460337414895	24.85225122641035
66-67	23.669164533810363	25.330133677392404	26.141250597825984	24.859451190971253
68-69	23.76502525541979	25.238051117071542	26.230660436232295	24.766263191276373
70-71	23.66975722134068	25.117766242763377	26.211903464155	25.000573071740938
72-73	23.69505283470831	25.30801075528168	26.089295457089122	24.907640952920886
74-75	23.715991234277702	25.417617573474217	26.10959381718868	24.756797375059403
76-77	23.781607987722953	25.16290331824173	26.14492393706102	24.910564756974292
78-79	23.733204341315936	25.268752830192287	26.199587548236668	24.798455280255112
80-81	23.89349669297056	25.35172668578085	26.046573102190273	24.708203519058316
82-83	23.852061269684608	25.378580324380724	26.052084423129685	24.717273982804986
84-85	23.868238974733448	25.35164440942831	26.050960474845148	24.7291561409931
86-87	23.889731541657483	25.440110002815686	26.0083272921394	24.661831163387436
88-89	23.744812301706546	25.244162809285232	26.070579609680294	24.94044527932792
90-91	23.835144598922746	25.19906031523172	26.113964568641467	24.851830517204064
92-93	23.98590941447948	25.102868599875467	26.176766140552317	24.73445584509274
94-95	23.96747401405665	24.973435954213013	26.194824661372117	24.86426537035822
96-97	24.042650581915932	24.818634051215994	26.15756468413577	24.981150682732306
98-99	23.928128866446478	24.837323581746222	26.08813917351987	25.146408378287433
100-101	24.148619434089497	24.77093560204638	26.205667896519113	24.874777067345015
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	57.0
1	52.5
2	98.0
3	157.0
4	146.5
5	167.0
6	251.5
7	423.5
8	645.0
9	728.5
10	645.5
11	573.5
12	611.5
13	694.5
14	795.5
15	915.0
16	1076.0
17	1163.5
18	1231.0
19	1353.0
20	1471.5
21	1654.0
22	1835.0
23	1969.5
24	2190.0
25	2487.0
26	2941.0
27	3640.5
28	4724.0
29	6467.0
30	9075.0
31	13032.5
32	19131.5
33	24772.0
34	29947.5
35	36944.0
36	45759.5
37	55326.0
38	64802.5
39	74025.5
40	82609.0
41	90703.0
42	99095.5
43	107884.5
44	116416.0
45	125103.5
46	130700.0
47	135622.5
48	141135.5
49	143276.0
50	143096.5
51	142217.5
52	140912.0
53	139730.5
54	136018.0
55	127757.5
56	117447.5
57	105788.5
58	95686.0
59	84285.0
60	69632.0
61	57630.5
62	48190.0
63	37596.5
64	27369.5
65	20254.5
66	15531.0
67	12157.5
68	9402.5
69	7350.5
70	5682.5
71	4173.0
72	3050.5
73	2311.5
74	1809.0
75	1416.5
76	1066.5
77	749.5
78	485.5
79	313.5
80	231.0
81	177.5
82	130.5
83	113.5
84	96.5
85	78.5
86	64.0
87	51.0
88	43.5
89	42.5
90	38.0
91	29.5
92	30.0
93	24.0
94	16.0
95	13.0
96	11.5
97	12.5
98	12.0
99	8.0
100	6.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05265596429690464
2	0.07283492466415536
3	0.06972069141062691
4	0.06965713562994266
5	0.06511289731101848
6	0.009469811321953882
7	0.012774711917535103
8	0.016556280868248233
9	0.006355578068425425
10-11	0.012568155630311278
12-13	0.02200618906192303
14-15	0.03816524630089467
16-17	0.02615320375157062
18-19	0.010391370141875569
20-21	0.019416290999039673
22-23	0.03586134925109046
24-25	0.033748119543339006
26-27	0.02025840509310604
28-29	0.010772704825981095
30-31	0.011312928961797256
32-33	0.008246362543781988
34-35	0.005418130303332674
36-37	8.421140940663688E-4
38-39	0.004162903634818653
40-41	0.0012393377233429577
42-43	0.22184145247838943
44-45	0.19282823859602738
46-47	0.1988660377610315
48-49	0.20663573194968163
50-51	0.19743603269563584
52-53	0.2050627263777463
54-55	0.20145593582391488
56-57	0.20404583388679826
58-59	0.21267353111468576
60-61	0.21601020960060913
62-63	0.20220271624695488
64-65	0.20261582882140253
66-67	0.20007359759403237
68-69	0.19479846779723928
70-71	0.1866633278696547
72-73	0.19846881413175493
74-75	0.19700703117601712
76-77	0.19136645564028953
78-79	0.2096069646966705
80-81	0.22052267002919115
82-83	0.21991889011269075
84-85	0.21299131001810703
86-87	0.2315337090327382
88-89	0.22506690834811538
90-91	0.23129537485517226
92-93	0.22743436117860383
94-95	0.2307074838838429
96-97	0.23741261874603176
98-99	0.2304055939255927
100-101	0.23645928203576794
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3146842.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.7666088269346
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.64590084598993	48.398113013770896
2	11.193302514124895	13.603580707147422
3	3.6166928624773393	6.593224812639805
4	1.6967910488888842	4.1243295171549965
5	0.9338350860769685	2.8372995692252974
6	0.6350764855169608	2.315486662235613
7	0.42697615628231045	1.8162125147064678
8	0.3005484318122638	1.4610647191587571
9	0.23847260683151142	1.3042054453742846
>10	1.2472396330796574	13.231793170560449
>50	0.050724517092169026	2.0540335773577043
>100	0.012971318734871323	1.3830266700218277
>500	9.964774559893589E-4	0.43400247588889584
>1k	4.7201563630832423E-4	0.44362714475764936
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.177789034212712E-5
2	0.0	0.0	0.0	0.0	3.177789034212712E-5
3	0.0	0.0	0.0	0.0	3.177789034212712E-5
4	0.0	0.0	0.0	0.0	3.177789034212712E-5
5	0.0	0.0	0.0	0.0	3.177789034212712E-5
6	0.0	0.0	0.0	0.0	6.355578068425425E-5
7	0.0	0.0	0.0	0.0	6.355578068425425E-5
8	0.0	0.0	0.0	0.0	9.533367102638137E-5
9	0.0	0.0	0.0	0.0	1.9066734205276274E-4
10-11	0.0	0.0	0.0	0.0	1.9066734205276274E-4
12-13	0.0	0.0	0.0	0.0	3.0188995825020765E-4
14-15	0.0	0.0	0.0	0.0	4.607794099608433E-4
16-17	0.0	0.0	0.0	0.0	5.402241358161611E-4
18-19	0.0	0.0	0.0	4.7666835513190684E-5	5.720020261582883E-4
20-21	0.0	0.0	0.0	9.533367102638137E-5	5.720020261582883E-4
22-23	0.0	0.0	0.0	1.5888945171063561E-4	6.673356971846696E-4
24-25	0.0	0.0	0.0	3.495567937633983E-4	7.944472585531781E-4
26-27	0.0	3.177789034212712E-5	0.0	9.215588199216866E-4	8.580030392374323E-4
28-29	0.0	3.177789034212712E-5	0.0	0.005831242877780326	8.897809295795594E-4
30-31	0.0	3.177789034212712E-5	0.0	0.027789765104190168	9.056698747506231E-4
32-33	0.0	3.177789034212712E-5	0.0	0.0783166107481723	9.215588199216866E-4
34-35	0.0	3.177789034212712E-5	0.0	0.14916541726594473	9.533367102638137E-4
36-37	0.0	3.177789034212712E-5	0.0	0.2627078194583649	9.533367102638137E-4
38-39	0.0	4.7666835513190684E-5	0.0	0.43689832536873474	0.0010327814361191315
40-41	0.0	6.355578068425425E-5	0.0	0.6278675573797476	0.001048670381290195
42-43	0.0	6.355578068425425E-5	0.0	0.8292281595326362	0.0010645593264612587
44-45	0.0	6.355578068425425E-5	0.0	1.0760470338199375	0.0010963372168033858
46-47	0.0	6.355578068425425E-5	0.0	1.368880293322639	0.0011916708878297673
48-49	0.0	6.355578068425425E-5	0.0	1.6541345259787432	0.0013346713943693392
50-51	0.0	6.355578068425425E-5	0.0	1.9507493544321577	0.0013982271750535935
52-53	0.0	6.355578068425425E-5	0.0	2.2541328735284454	0.0014776719009089111
54-55	0.0	6.355578068425425E-5	0.0	2.6078366819814915	0.0016365613526195468
56-57	0.0	6.355578068425425E-5	0.0	3.013020672788783	0.0016842281881327375
58-59	0.0	6.355578068425425E-5	0.0	3.4237340165156054	0.001700117133303801
60-61	0.0	6.355578068425425E-5	0.0	3.843710615277157	0.0017477839688169918
62-63	0.0	9.533367102638137E-5	0.0	4.280100494400418	0.0018431176398433732
64-65	0.0	9.533367102638137E-5	0.0	4.760312084305472	0.0018748955301855002
66-67	0.0	9.533367102638137E-5	0.0	5.315185827569353	0.0019066734205276275
68-69	0.0	9.533367102638137E-5	0.0	5.898802672647689	0.001954340256040818
70-71	0.0	9.533367102638137E-5	0.0	6.489919099846767	0.002049673927067199
72-73	0.0	9.533367102638137E-5	0.0	7.100960264290358	0.0022721191594620893
74-75	0.0	9.533367102638137E-5	0.0	7.74457376633463	0.002288008104633153
76-77	0.0	9.533367102638137E-5	0.0	8.479707592564228	0.0023038970498042164
78-79	0.0	9.533367102638137E-5	0.0	9.260681025612344	0.00231978599497528
80-81	0.0	9.533367102638137E-5	0.0	10.057162069147418	0.002335674940146344
82-83	0.0	9.533367102638137E-5	0.0	10.869706836250437	0.002383341775659534
84-85	0.0	1.1122261619744493E-4	0.0	11.72859965641745	0.002383341775659534
86-87	3.177789034212712E-5	1.271115613685085E-4	0.0	12.65379704478331	0.0024786754466859153
88-89	3.177789034212712E-5	1.271115613685085E-4	0.0	13.630156836599994	0.00254223122737017
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3660	0.0	42.14215	1
GGTATCA	2115	0.0	41.73659	1
TCAACGC	4425	0.0	34.75152	4
CAACGCA	4445	0.0	34.380516	5
ATCAACG	4690	0.0	32.68519	3
TATCAAC	4670	0.0	32.31704	2
AACGCAG	4815	0.0	31.722967	6
GTACATG	7080	0.0	28.354471	1
ACGCAGA	5810	0.0	25.88485	7
TACATGG	7665	0.0	25.633595	2
ACATGGG	7980	0.0	24.502796	3
CGCAGAG	6145	0.0	24.166052	8
CATGGGG	6775	0.0	22.627432	4
GAGTACA	5365	0.0	19.991858	1
GCAGAGT	7170	0.0	19.389791	9
TATACCG	150	7.7689614E-4	18.984022	5
ATGGGGG	4000	0.0	18.153471	5
AGAGTAC	5845	0.0	18.103058	10-11
AGTACAT	5735	0.0	17.212788	2
TAAGGCG	995	0.0	17.171476	5
>>END_MODULE