FastQCFastQC Report
Mon 15 Aug 2016
SRR2921652_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921652_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3146842
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20550.043.7219161
GTATCAA36550.040.1902121
TCAACGC44550.032.6016044
CAACGCA46150.031.7001935
ATCAACG46900.031.2721563
TATCAAC48250.030.4955562
AACGCAG49650.029.4631966
GTACATG72350.028.8453081
TACATGG77200.026.499162
ACATGGG80250.025.2554443
ACGCAGA58850.024.3726067
CGCAGAG62400.022.605468
CATGGGG66350.022.1761444
GAGTACA54400.019.0505181
ATGGGGG37900.018.4230295
CCCTATA4253.6379788E-1217.8691232
AGAGTAC59150.017.70497510-11
GCAGAGT73050.017.4893469
GTATAGT7000.016.9781821
GTATAAA10650.016.5158631
Produced by FastQC (version 0.11.5)