##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921652_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3146842
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.34226281459317	33.0	31.0	34.0	28.0	34.0
2	31.46858151759764	34.0	31.0	34.0	28.0	34.0
3	31.545860262447242	34.0	31.0	34.0	28.0	34.0
4	35.019728667661106	37.0	35.0	37.0	32.0	37.0
5	34.751905243415464	37.0	35.0	37.0	32.0	37.0
6	34.72093514704583	37.0	35.0	37.0	32.0	37.0
7	34.59759307902971	37.0	35.0	37.0	32.0	37.0
8	34.54267866006619	37.0	35.0	37.0	32.0	37.0
9	36.1290055236329	39.0	37.0	39.0	32.0	39.0
10-11	36.02103982341662	39.0	37.0	39.0	31.0	39.0
12-13	35.940673062073024	39.0	37.0	39.0	31.0	39.0
14-15	37.174926640740146	40.0	38.0	41.0	31.0	41.0
16-17	37.13885047295034	40.0	38.0	41.0	31.0	41.0
18-19	37.09151460416507	40.0	38.0	41.0	31.0	41.0
20-21	37.02587864277901	40.0	38.0	41.0	31.0	41.0
22-23	36.934911253885645	40.0	37.0	41.0	30.0	41.0
24-25	36.8428365644033	40.0	37.0	41.0	30.0	41.0
26-27	36.713214231918855	40.0	37.0	41.0	30.0	41.0
28-29	36.58798900612106	40.0	37.0	41.0	30.0	41.0
30-31	36.43001237431049	40.0	37.0	41.0	30.0	41.0
32-33	36.377062464527924	40.0	36.5	41.0	29.5	41.0
34-35	36.5020341027608	40.0	37.0	41.0	30.0	41.0
36-37	36.49044200503235	40.0	37.0	41.0	30.0	41.0
38-39	36.380011293862225	40.0	37.0	41.0	29.5	41.0
40-41	36.29039160529827	40.0	36.5	41.0	29.0	41.0
42-43	36.16316484907726	40.0	36.0	41.0	28.5	41.0
44-45	36.026365638948505	40.0	36.0	41.0	28.0	41.0
46-47	35.86984983675698	40.0	35.5	41.0	27.5	41.0
48-49	35.71922200097748	40.0	35.0	41.0	27.0	41.0
50-51	35.555038511625305	39.5	35.0	41.0	27.0	41.0
52-53	35.36625607513819	39.0	35.0	41.0	26.0	41.0
54-55	35.14530408581047	39.0	35.0	41.0	26.0	41.0
56-57	34.913570017179126	39.0	35.0	41.0	26.0	41.0
58-59	34.66065693797147	38.0	34.5	40.0	25.5	41.0
60-61	34.37676836015281	38.0	34.0	40.0	25.0	41.0
62-63	34.06758299272731	37.0	34.0	40.0	23.5	41.0
64-65	33.722204991543904	37.0	34.0	40.0	23.0	41.0
66-67	33.36789247760135	36.0	34.0	39.0	22.0	41.0
68-69	33.00574178811647	36.0	33.0	39.0	22.0	40.5
70-71	32.606247628574934	35.0	33.0	38.5	21.0	40.0
72-73	32.246185064264424	35.0	33.0	37.0	20.0	39.5
74-75	31.84936008862218	35.0	32.5	37.0	19.0	39.0
76-77	29.152122032183378	33.0	28.5	34.5	11.5	36.5
78-79	30.72312083034356	34.5	31.5	35.5	12.5	37.0
80-81	30.909926999830304	35.0	32.0	35.0	15.0	37.0
82-83	30.742053461851597	35.0	32.0	35.0	11.5	37.0
84-85	30.5515875916236	35.0	32.0	35.0	8.5	36.0
86-87	30.35053793612771	35.0	32.0	35.0	7.0	36.0
88-89	30.08920625821061	35.0	31.0	35.0	2.0	35.5
90-91	29.897294811750953	34.0	31.0	35.0	2.0	35.0
92-93	29.74262975389295	34.0	31.0	35.0	2.0	35.0
94-95	29.635604361451893	34.0	31.0	35.0	2.0	35.0
96-97	29.431659581256383	34.0	31.0	35.0	2.0	35.0
98-99	29.224252758797547	34.0	31.0	35.0	2.0	35.0
100-101	28.09568720005644	33.0	28.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	61085.0
3	21592.0
4	11768.0
5	8330.0
6	7126.0
7	7394.0
8	7752.0
9	8220.0
10	8984.0
11	9746.0
12	10306.0
13	10915.0
14	11785.0
15	12384.0
16	13122.0
17	13851.0
18	13407.0
19	12326.0
20	11983.0
21	11792.0
22	12472.0
23	13874.0
24	16610.0
25	19845.0
26	23677.0
27	28992.0
28	34674.0
29	41592.0
30	50953.0
31	62726.0
32	79885.0
33	104758.0
34	149106.0
35	224512.0
36	396734.0
37	754650.0
38	770398.0
39	87514.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.05629757325095	18.800414038871526	12.39621177349833	26.747076614379196
2	15.837167340676578	20.758424806106955	36.59679970815149	26.807608145064982
3	18.320112861587237	23.829075333965356	28.79397452859661	29.056837275850793
4	13.345941483015864	16.01008312988894	34.552675518854706	36.0912998682405
5	14.395601880694404	36.88729946422078	34.207799440063674	14.509299215021143
6	34.861774531989695	34.75528468951903	16.265775257811242	14.117165520680036
7	29.508567637015144	31.245642456786836	20.649559145327284	18.59623076087074
8	26.990169827401566	34.52632829992736	19.636289333878217	18.84721253879286
9	26.798462330953488	14.98299405657928	18.851889879406045	39.366653733061185
10-11	25.910341458622415	24.824689314636284	27.765813887795122	21.49915533894618
12-13	26.345401562842607	22.264564657131146	27.36951154018488	24.020522239841362
14-15	23.481875428028456	24.051202928787188	24.39963199483903	28.06728964834533
16-17	23.944878706970353	27.015751029126978	25.325516819719578	23.71385344418309
18-19	23.899635788622025	26.420133602432184	26.538698531428285	23.141532077517503
20-21	24.34975183017106	25.725816113132012	26.379599578878725	23.544832477818204
22-23	24.28429835371461	25.467373322206832	26.110748490073544	24.137579834005013
24-25	24.04030100228167	25.584161153164146	26.187230283270097	24.188307561284088
26-27	23.84798296021057	25.815487554232497	26.232806679266957	24.103722806289976
28-29	24.054406034564124	26.113104221506333	25.951886325652524	23.88060341827702
30-31	24.239869079337755	25.82500008740334	25.804500032259774	24.130630800999132
32-33	23.990390559127967	25.889507320699355	25.715551524254494	24.40455059591818
34-35	24.31110000896223	25.85265139019456	25.871767637956385	23.96448096288683
36-37	24.361369815606146	25.661407902938794	25.91646750397359	24.06075477748147
38-39	24.156586850656446	25.844994099639273	25.94128112267491	24.057137927029373
40-41	24.33195386007549	25.630252968754096	26.005483465785538	24.032309705384872
42-43	24.201482029522047	25.5776023588909	25.791422928537756	24.4294926830493
44-45	24.04357031911048	25.699147105441924	25.951003253472955	24.306279321974646
46-47	24.145290623353947	25.649488238177597	25.83690516576348	24.368315972704977
48-49	23.893009247209104	25.719346552167423	25.906963485012735	24.480680715610738
50-51	23.986760362950754	25.76206088605974	25.946742732134574	24.30443601885493
52-53	24.204288011877566	25.559231631897184	25.87909900078588	24.357381355439365
54-55	23.665235863282273	25.52897194123272	26.102966997032144	24.702825198452864
56-57	23.733827281572264	25.5972244490395	25.992347022684832	24.676601246703406
58-59	23.74390351846699	25.50190544099108	26.00780355992727	24.746387480614654
60-61	23.707008946241988	25.51627877266096	26.140200535271322	24.636511745825732
62-63	23.73678010912191	25.54084189020045	26.060563103623856	24.661814897053784
64-65	23.510381626316768	25.423273783514556	25.942406758329295	25.12393783183938
66-67	23.521064264891027	25.544064853284087	25.827832152334185	25.107038729490704
68-69	23.543983330196404	25.457313195952853	25.88129123576301	25.117412238087727
70-71	23.427854774186297	25.248702489195164	25.881880448229705	25.441562288388837
72-73	23.37630157174046	25.367186596020357	25.8121432558589	25.444368576380278
74-75	23.322984352563576	25.445206159802353	25.7793084486303	25.452501039003767
76-77	23.35100612958437	25.138939581267156	25.933451045812962	25.576603243335516
78-79	23.195419721569095	25.165881480016143	25.995360438996357	25.643338359418404
80-81	23.266205296556006	25.23699169050469	25.857342004479726	25.639461008459584
82-83	23.150984613159594	25.245924827540723	25.896235973404597	25.70685458589509
84-85	23.092778293628708	25.169710187157712	25.87449450185571	25.863017017357866
86-87	23.048140186058507	25.245380137488876	25.805215559135053	25.901264117317567
88-89	22.88040832489429	25.030790890453908	25.888776722421458	26.200024062230348
90-91	22.722906265776633	24.90659915755978	25.983919947780944	26.38657462888265
92-93	22.784004405403298	24.759822696506788	26.094908525723966	26.36126437236595
94-95	22.60519588698276	24.662943960942577	26.158710827887017	26.573149324187646
96-97	22.58312880047737	24.49846213010276	26.14312082310007	26.7752882463198
98-99	22.37406711230244	24.533425066297195	26.117598269996805	26.974909551403563
100-101	22.251140194328773	24.433970418503435	26.205784102869174	27.109105284298625
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	63.0
1	56.5
2	78.5
3	115.0
4	112.0
5	128.5
6	206.5
7	418.5
8	626.0
9	620.5
10	502.0
11	440.0
12	436.0
13	437.0
14	453.0
15	495.0
16	560.0
17	646.5
18	730.5
19	800.0
20	917.5
21	1045.0
22	1191.0
23	1427.0
24	1716.5
25	2059.0
26	2559.0
27	3314.5
28	4459.0
29	6279.0
30	9199.5
31	13176.5
32	19430.5
33	24970.0
34	29802.5
35	36560.5
36	44826.5
37	54326.0
38	63573.5
39	72580.5
40	81367.0
41	90008.0
42	98516.0
43	106778.0
44	115201.0
45	124101.5
46	130364.0
47	135579.0
48	141182.5
49	143801.5
50	144040.5
51	143520.5
52	143212.0
53	142807.0
54	139561.0
55	131505.5
56	120401.5
57	108191.5
58	97610.5
59	85742.0
60	70781.0
61	58121.0
62	48101.5
63	37212.5
64	26740.0
65	19644.5
66	15028.0
67	11705.0
68	9024.0
69	6946.0
70	5251.0
71	3860.5
72	2783.0
73	2059.5
74	1567.5
75	1168.5
76	805.0
77	496.5
78	293.0
79	165.5
80	107.5
81	62.0
82	37.0
83	23.5
84	15.5
85	11.5
86	9.0
87	6.5
88	5.5
89	5.5
90	3.0
91	1.5
92	1.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16232146386758536
2	1.271115613685085E-4
3	0.010582037483928332
4	0.006355578068425425
5	0.024468975563437884
6	0.002002007091554009
7	0.0
8	0.0
9	3.177789034212712E-5
10-11	0.04758739078733537
12-13	3.813346841055255E-4
14-15	0.004671349880292687
16-17	0.0
18-19	0.0013664492847114663
20-21	1.5888945171063561E-4
22-23	0.0
24-25	6.991135875267966E-4
26-27	0.0028441211856203774
28-29	0.005370463467819484
30-31	0.016174946184142707
32-33	0.011217595290770875
34-35	0.009660478664006645
36-37	0.004019903128279081
38-39	1.588894517106356E-5
40-41	0.0034796789924629195
42-43	0.008850142460282404
44-45	0.006498578574964997
46-47	0.003638568444173556
48-49	0.00861180828271645
50-51	0.002510453337028043
52-53	0.0021926744336067715
54-55	0.00397223629276589
56-57	0.007102358491465412
58-59	0.014538384831523157
60-61	0.006784579588044141
62-63	0.02021073825759285
64-65	0.03853069203982914
66-67	0.05832831772297433
68-69	0.04811172597798046
70-71	0.06579612195337421
72-73	0.03273122705239094
74-75	0.025406423328530634
76-77	0.22206389771078436
78-79	0.08195517919234585
80-81	0.06374644802630701
82-83	0.025724202231951904
84-85	0.04936695264649448
86-87	0.06605034507611122
88-89	0.29059291823358147
90-91	0.29521660127836097
92-93	0.05127362606702211
94-95	0.009390366596098564
96-97	0.014125272257075506
98-99	8.580030392374323E-4
100-101	3.1777890342127123E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3146842.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.18807252959629
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.07639114491818	47.59455565609061
2	11.48731615076418	13.827988353051948
3	3.7773902436778015	6.820615138772068
4	1.7272335211478946	4.1583542578559785
5	0.9955400603204737	2.995981867834367
6	0.6076602929673235	2.1944341071883797
7	0.4308839366242269	1.8153851540563857
8	0.32306092865405933	1.5555531684247403
9	0.2289129623231946	1.2400046981345862
>10	1.2768897038516696	13.392418503800457
>50	0.054236980507638276	2.1602033591203873
>100	0.01284199836744328	1.3300418997580925
>500	0.001112373981415309	0.4588738278307054
>1k	5.297018944940762E-4	0.4555900080812497
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.355578068425425E-5
2	0.0	0.0	0.0	0.0	6.355578068425425E-5
3	0.0	0.0	0.0	0.0	6.355578068425425E-5
4	0.0	0.0	0.0	0.0	6.355578068425425E-5
5	0.0	0.0	0.0	0.0	6.355578068425425E-5
6	0.0	0.0	0.0	0.0	1.5888945171063561E-4
7	0.0	0.0	0.0	0.0	1.9066734205276274E-4
8	0.0	0.0	0.0	0.0	2.2244523239488986E-4
9	0.0	0.0	0.0	0.0	3.495567937633984E-4
10-11	0.0	0.0	0.0	0.0	3.495567937633984E-4
12-13	0.0	1.588894517106356E-5	0.0	0.0	4.7666835513190687E-4
14-15	0.0	3.177789034212712E-5	0.0	0.0	6.037799165004153E-4
16-17	0.0	3.177789034212712E-5	0.0	0.0	6.991135875267968E-4
18-19	0.0	3.177789034212712E-5	0.0	3.177789034212712E-5	7.785583133821145E-4
20-21	0.0	3.177789034212712E-5	0.0	3.177789034212712E-5	9.056698747506231E-4
22-23	0.0	3.177789034212712E-5	0.0	9.533367102638137E-5	0.0010327814361191315
24-25	0.0	3.177789034212712E-5	0.0	2.8600101307914413E-4	0.0012075598330008306
26-27	0.0	3.177789034212712E-5	0.0	8.738919844084959E-4	0.0012075598330008306
28-29	0.0	3.177789034212712E-5	0.0	0.005990132329490963	0.0012075598330008306
30-31	0.0	3.177789034212712E-5	0.0	0.028123432952782506	0.0012234487781718944
32-33	0.0	3.177789034212712E-5	0.0	0.07922228062292291	0.001271115613685085
34-35	0.0	3.177789034212712E-5	0.0	0.15070664494753788	0.0013346713943693392
36-37	0.0	3.177789034212712E-5	0.0	0.2649004938919717	0.0013346713943693392
38-39	0.0	3.177789034212712E-5	0.0	0.4382647746534462	0.0013346713943693392
40-41	0.0	3.177789034212712E-5	0.0	0.6292975624451435	0.0013664492847114663
42-43	0.0	3.177789034212712E-5	0.0	0.8308170540497426	0.0013664492847114663
44-45	0.0	3.177789034212712E-5	0.0	1.07712748209157	0.0014617829557378478
46-47	0.0	3.177789034212712E-5	0.0	1.369388739568113	0.001557116626764229
48-49	0.0	3.177789034212712E-5	0.0	1.6545476385531908	0.0016206724074484832
50-51	0.0	3.177789034212712E-5	0.0	1.9519886921555007	0.0016206724074484832
52-53	0.0	3.177789034212712E-5	0.0	2.2554357670324725	0.0017160060784748646
54-55	0.0	3.177789034212712E-5	0.0	2.6091395754855187	0.001811339749501246
56-57	0.0	3.177789034212712E-5	0.0	3.0138310089925073	0.001811339749501246
58-59	0.0	3.177789034212712E-5	0.0	3.424655575335527	0.0018431176398433732
60-61	0.0	6.355578068425425E-5	0.0	3.844663951987421	0.0019066734205276275
62-63	0.0	6.355578068425425E-5	0.0	4.280481829084524	0.0019702292012118818
64-65	0.0	6.355578068425425E-5	0.0	4.760931753167144	0.002002007091554009
66-67	0.0	6.355578068425425E-5	0.0	5.315614829088972	0.002033784981896136
68-69	0.0	6.355578068425425E-5	0.0	5.898198892731189	0.002065562872238263
70-71	0.0	6.355578068425425E-5	0.0	6.488886318410648	0.002065562872238263
72-73	0.0	6.355578068425425E-5	0.0	7.101341598974464	0.0021450075980935807
74-75	0.0	6.355578068425425E-5	0.0	7.747481443300934	0.002176785488435708
76-77	1.588894517106356E-5	6.355578068425425E-5	0.0	8.483759273582848	0.0021926744336067715
78-79	3.177789034212712E-5	6.355578068425425E-5	0.0	9.270055503263272	0.0021926744336067715
80-81	3.177789034212712E-5	6.355578068425425E-5	0.0	10.070445227310428	0.002208563378777835
82-83	3.177789034212712E-5	6.355578068425425E-5	0.0	10.885103224121199	0.002351563885317407
84-85	3.177789034212712E-5	6.355578068425425E-5	0.0	11.749016950962266	0.0024151196660016612
86-87	3.177789034212712E-5	6.355578068425425E-5	0.0	12.680140915876933	0.0024310086111727245
88-89	3.177789034212712E-5	6.355578068425425E-5	0.0	13.66266561841999	0.0024468975563437883
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2055	0.0	43.721916	1
GTATCAA	3655	0.0	40.190212	1
TCAACGC	4455	0.0	32.601604	4
CAACGCA	4615	0.0	31.700193	5
ATCAACG	4690	0.0	31.272156	3
TATCAAC	4825	0.0	30.495556	2
AACGCAG	4965	0.0	29.463196	6
GTACATG	7235	0.0	28.845308	1
TACATGG	7720	0.0	26.49916	2
ACATGGG	8025	0.0	25.255444	3
ACGCAGA	5885	0.0	24.372606	7
CGCAGAG	6240	0.0	22.60546	8
CATGGGG	6635	0.0	22.176144	4
GAGTACA	5440	0.0	19.050518	1
ATGGGGG	3790	0.0	18.423029	5
CCCTATA	425	3.6379788E-12	17.869123	2
AGAGTAC	5915	0.0	17.704975	10-11
GCAGAGT	7305	0.0	17.489346	9
GTATAGT	700	0.0	16.978182	1
GTATAAA	1065	0.0	16.515863	1
>>END_MODULE