FastQCFastQC Report
Mon 15 Aug 2016
SRR2921651_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921651_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3161211
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21600.054.2683871
GTATCAA41800.043.0295371
TCAACGC48200.035.9399154
CAACGCA49450.035.221155
ATCAACG51400.033.794753
TATCAAC53050.032.8331032
AACGCAG54150.032.2328536
GTACATG80100.028.6166211
ACGCAGA62550.027.2286767
TACATGG85000.026.1869622
CGCAGAG67350.024.7257548
ACATGGG88750.024.492233
CATGGGG70700.022.6225594
GCAGAGT79050.020.0461719
CAGAGTA70600.018.24577510-11
GAGTACA60600.018.0901891
TAATACT23100.018.0801474
AGAGTAC63500.017.89488810-11
ATGGGGG41050.017.2256985
TTAATAC22600.016.8001353
Produced by FastQC (version 0.11.5)