##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921651_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3161211
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.47314589250765	31.0	31.0	34.0	26.0	34.0
2	30.523698038504865	31.0	31.0	34.0	26.0	34.0
3	30.586877307462235	33.0	31.0	34.0	26.0	34.0
4	34.05527470327036	37.0	35.0	37.0	30.0	37.0
5	34.05188739378675	37.0	35.0	37.0	30.0	37.0
6	34.100234688541825	37.0	35.0	37.0	30.0	37.0
7	34.01291783433627	37.0	35.0	37.0	30.0	37.0
8	33.98205371296	37.0	35.0	37.0	30.0	37.0
9	35.546072691762745	39.0	37.0	39.0	30.0	39.0
10-11	35.44793767325243	39.0	36.0	39.0	30.0	39.0
12-13	35.3432540567523	39.0	35.0	39.0	29.0	39.0
14-15	36.49402507456794	40.0	37.0	41.0	29.0	41.0
16-17	36.43377253210873	40.0	37.0	41.0	29.0	41.0
18-19	36.400892411167746	40.0	37.0	41.0	29.0	41.0
20-21	36.32441855352268	40.0	37.0	41.0	28.0	41.0
22-23	36.22535398617808	40.0	36.5	41.0	27.5	41.0
24-25	36.116159914665616	40.0	36.0	41.0	27.0	41.0
26-27	35.968473157913216	40.0	36.0	41.0	27.0	41.0
28-29	35.82866597642486	40.0	36.0	41.0	27.0	41.0
30-31	35.681175663377104	40.0	36.0	41.0	26.5	41.0
32-33	35.50913241792465	39.5	35.5	41.0	25.5	41.0
34-35	35.332036994683364	39.0	35.0	41.0	25.0	41.0
36-37	35.29542159634393	39.0	35.0	41.0	25.0	41.0
38-39	35.467592482754235	40.0	35.0	41.0	25.5	41.0
40-41	35.53015094531811	40.0	35.0	41.0	25.0	41.0
42-43	35.36622879649603	40.0	35.0	41.0	24.5	41.0
44-45	35.239754638333224	40.0	35.0	41.0	24.0	41.0
46-47	35.08311435079784	40.0	35.0	41.0	23.5	41.0
48-49	34.91752938984459	39.0	35.0	41.0	23.0	41.0
50-51	33.20224069193736	37.0	32.5	39.5	19.0	40.0
52-53	33.32114591528373	37.5	33.0	39.5	19.5	40.0
54-55	34.23064167497836	39.0	34.0	40.5	21.0	41.0
56-57	34.2488546319749	39.0	34.0	41.0	21.0	41.0
58-59	34.05633537274165	38.0	34.0	41.0	20.0	41.0
60-61	33.799684677802276	38.0	34.0	40.0	19.0	41.0
62-63	33.50314120126749	37.0	34.0	40.0	18.0	41.0
64-65	33.212294434000135	37.0	33.0	40.0	16.0	41.0
66-67	32.866583565601914	36.0	33.0	39.0	13.0	41.0
68-69	32.503442351681045	36.0	33.0	39.0	10.0	41.0
70-71	32.092974179831714	35.0	32.5	39.0	7.5	40.5
72-73	31.725713974802694	35.0	32.0	37.5	4.0	40.0
74-75	31.32615428074874	35.0	32.0	37.0	2.0	39.0
76-77	30.948005526995825	35.0	32.0	36.5	2.0	39.0
78-79	30.596765448430997	35.0	31.0	36.0	2.0	38.5
80-81	30.27467211141553	35.0	31.0	36.0	2.0	37.0
82-83	29.953085858552306	35.0	31.0	35.0	2.0	37.0
84-85	29.683249710316712	35.0	31.0	35.0	2.0	36.0
86-87	29.43349431594411	35.0	31.0	35.0	2.0	36.0
88-89	29.191255819367957	35.0	30.5	35.0	2.0	36.0
90-91	28.9479612401703	34.0	30.0	35.0	2.0	35.5
92-93	28.7392760242831	34.0	30.0	35.0	2.0	35.0
94-95	28.484133612087266	34.0	30.0	35.0	2.0	35.0
96-97	28.223051071250858	34.0	29.0	35.0	2.0	35.0
98-99	27.924998521136363	34.0	29.0	35.0	2.0	35.0
100-101	26.818139788834088	33.0	26.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	99845.0
3	17609.0
4	12134.0
5	9449.0
6	8684.0
7	9018.0
8	9558.0
9	10349.0
10	11262.0
11	12057.0
12	12186.0
13	12248.0
14	12908.0
15	13770.0
16	13869.0
17	14162.0
18	14004.0
19	13452.0
20	13529.0
21	14241.0
22	15656.0
23	17859.0
24	20839.0
25	24799.0
26	29489.0
27	35168.0
28	41071.0
29	49173.0
30	58238.0
31	70602.0
32	87579.0
33	113096.0
34	155913.0
35	226850.0
36	377436.0
37	674777.0
38	705479.0
39	122846.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.894600396829034	17.69905622546523	12.285811858472115	25.120531519233612
2	16.265412283734175	19.47668197606466	38.56049871408654	25.697407026114615
3	19.21167108686142	23.386301806972238	29.260560192416175	28.141466913750175
4	13.5242841165825	15.37306596171762	36.07389555995428	35.0287543617456
5	14.404721582977484	36.87582895182504	34.308365330741886	14.41108413445559
6	34.107314881749886	34.906363249532966	16.81797977465172	14.168342094065428
7	29.32468820166687	31.39569795654676	20.917388351028876	18.362225490757496
8	26.663083016758254	34.35418842510609	20.066846988167946	18.915881569967706
9	26.784015569751478	15.30470885646667	19.35348960661512	38.55778596716673
10-11	25.693056017595538	24.800197038630866	28.054925309292596	21.451821634481
12-13	26.20589725197588	22.44823506742153	27.54818953649261	23.797678144109977
14-15	23.430133388186523	24.130492571084986	24.68632415069859	27.753049890029907
16-17	23.703545961725972	27.082405493203822	25.682208710672565	23.531839834397644
18-19	23.71372519988155	26.476744392053835	26.826125828568827	22.983404579495783
20-21	24.15681952867258	25.829432920653694	26.675168316530947	23.338579234142777
22-23	24.08339394752586	25.600887464563048	26.4303775917413	23.885340996169795
24-25	23.877320629124167	25.756006718125885	26.426553527055646	23.940119125694302
26-27	23.706672480002684	25.924831413782854	26.422830337387587	23.94566576882687
28-29	23.84995297149595	26.11102270307756	26.290246702705506	23.748777622720986
30-31	24.10037067637167	25.8272831286965	26.079304464140662	23.993041730791166
32-33	23.78424191115966	25.95660959167384	25.984449508356562	24.274698988809934
34-35	24.21279639459404	25.94669270880292	26.035951561373487	23.80455933522955
36-37	24.353893704151904	25.688171197744897	26.041693522353075	23.91624157575012
38-39	23.98156327776956	25.864826368815113	26.11148237984931	24.042127973566018
40-41	24.21797941901258	25.68988697222864	26.202956500283126	23.88917710847566
42-43	24.089210926900662	25.663701381858445	26.00511550469714	24.241972186543755
44-45	23.940825772840675	25.720128803345414	26.19190481307254	24.147140610741367
46-47	24.198925693226787	25.676981112697288	25.996822821165534	24.127270372910388
48-49	23.863623935379287	25.809958159353947	26.026428857983525	24.299989047283244
50-51	23.874082983686236	25.876854379069385	26.105733029007094	24.143329608237284
52-53	24.152966973328038	25.689401621994662	26.10011504160233	24.057516363074974
54-55	23.67565626190215	25.585152102696085	26.339488152091445	24.39970348331032
56-57	23.701406255795206	25.767344724931977	26.261758027380793	24.26949099189202
58-59	23.810000863906236	25.463279625841416	26.324094820446202	24.402624689806142
60-61	23.66793536758773	25.525855659774678	26.456884512284628	24.349324460352964
62-63	23.73559678576399	25.524735219240785	26.459071873342694	24.280596121652536
64-65	23.57269882932489	25.492314373658704	26.325053334981725	24.609933462034686
66-67	23.561330840097646	25.531268098174483	26.257937045059244	24.649464016668627
68-69	23.713659190172752	25.4873731346406	26.298148135821336	24.500819539365306
70-71	23.595184931040112	25.353119319703527	26.308035098784664	24.743660650471696
72-73	23.65257455495258	25.44991314601613	26.227620834812598	24.669891464218693
74-75	23.658659280565143	25.67705754553287	26.152039113033855	24.512244060868138
76-77	23.68031853658784	25.385000862143286	26.30326270520278	24.63141789606609
78-79	23.652682556298586	25.437729960796894	26.2724682358476	24.637119247056912
80-81	23.828349667244776	25.54204700742239	26.104100546295623	24.525502779037215
82-83	23.7700950956624	25.54750114496211	26.198936631796517	24.48346712757898
84-85	23.77921619253298	25.54613720435071	26.15449216991457	24.520154433201743
86-87	23.754356881208714	25.650234396403892	26.150435434445107	24.444973287942283
88-89	23.720149423507014	25.412919334103712	26.170246598249857	24.696684644139417
90-91	23.65184016397601	25.461610612779168	26.236447372426458	24.650101850818356
92-93	23.89363133706599	25.306742435916707	26.31048840980333	24.489137817213976
94-95	23.82628123589447	25.201022867386662	26.31747922706892	24.655216669649953
96-97	23.904868500184957	25.029273535883213	26.3113949487949	24.754463015136935
98-99	23.765482158012386	25.06162263939749	26.24651180267564	24.92638339991448
100-101	23.994097279148214	24.929276119159237	26.353948883464867	24.722677718227686
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	74.0
1	67.0
2	104.0
3	161.0
4	142.0
5	146.0
6	235.0
7	416.5
8	677.5
9	784.0
10	684.0
11	599.5
12	644.0
13	711.0
14	763.5
15	876.5
16	1035.5
17	1151.5
18	1247.5
19	1364.0
20	1471.5
21	1612.5
22	1744.0
23	1918.0
24	2165.5
25	2477.5
26	2980.0
27	3849.5
28	5222.5
29	7277.5
30	10679.0
31	15815.5
32	23536.0
33	30565.0
34	36549.5
35	44385.5
36	54309.5
37	64499.0
38	72869.5
39	81136.0
40	88481.0
41	94365.0
42	100825.0
43	108705.0
44	115642.0
45	123015.0
46	128619.0
47	131938.5
48	136439.0
49	138482.5
50	137754.0
51	136962.5
52	135418.5
53	133567.5
54	131086.0
55	124381.5
56	114394.0
57	103312.0
58	94062.0
59	83329.5
60	69101.0
61	57345.5
62	47919.5
63	37154.5
64	26851.5
65	19774.0
66	15119.5
67	11890.5
68	9148.0
69	7119.0
70	5600.5
71	4155.0
72	3029.0
73	2305.0
74	1845.5
75	1429.5
76	1037.5
77	705.0
78	467.0
79	321.5
80	245.0
81	184.0
82	125.5
83	106.0
84	91.5
85	72.0
86	67.0
87	55.5
88	41.5
89	35.5
90	32.0
91	26.5
92	23.0
93	20.5
94	17.0
95	13.5
96	10.5
97	10.5
98	10.5
99	7.5
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05263805547937167
2	0.0748763685815341
3	0.07038441913557811
4	0.06997318432714551
5	0.06646187173206723
6	0.010312503657617286
7	0.013728915912288045
8	0.017714730209403926
9	0.006643023828526473
10-11	0.012953896465626622
12-13	0.022839348591410066
14-15	0.03988977641796135
16-17	0.027505281994779848
18-19	0.010723738466049878
20-21	0.020498473528024544
22-23	0.03788105254600215
24-25	0.03490750854656649
26-27	0.020941341783259643
28-29	0.011451307742507539
30-31	0.011625291699921329
32-33	0.008920631998306979
34-35	0.0056940204244512625
36-37	7.750194466614219E-4
38-39	0.004064897914122152
40-41	0.0012969713189027875
42-43	0.22997515825422596
44-45	0.20004991757905433
46-47	0.20642405710975953
48-49	0.21328851506590354
50-51	0.20506381889725173
52-53	0.21194409357679697
54-55	0.20822716357750243
56-57	0.21080528949190672
58-59	0.2193305034051824
60-61	0.22352193510651458
62-63	0.20941341783259643
64-65	0.20944505127939894
66-67	0.2069460089820009
68-69	0.20213772506801983
70-71	0.19347016064413292
72-73	0.20533270319507305
74-75	0.2042571660037878
76-77	0.19875294626015158
78-79	0.21729014608642067
80-81	0.22905778829695328
82-83	0.2270174309781916
84-85	0.22039022387306634
86-87	0.23974989331620064
88-89	0.23271145140264285
90-91	0.23974989331620064
92-93	0.23487834250861456
94-95	0.23812077080587157
96-97	0.24564953114486823
98-99	0.23853200561430415
100-101	0.24515921271942936
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3161211.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.84584102613516
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.91073542684632	46.435685921836104
2	11.678830652221619	13.745012238635754
3	3.7433670840537907	6.608447529920894
4	1.7567574851210532	4.1351148676362595
5	0.9546481488545401	2.8088536601694236
6	0.6048086572406133	2.1354284457126833
7	0.4081204807641816	1.6811335051391219
8	0.316008845095365	1.4876645009067535
9	0.22990600767998828	1.2176111141000998
>10	1.303054864209882	13.644372587727757
>50	0.06854209574728112	2.7351443523090273
>100	0.023172685873842497	2.2064418690100065
>500	0.0014009664110564278	0.5768653699384096
>1k	6.465998805045961E-4	0.5822240369577054
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.326689360501403E-5
2	0.0	0.0	0.0	0.0	6.326689360501403E-5
3	0.0	0.0	0.0	0.0	6.326689360501403E-5
4	0.0	0.0	0.0	0.0	6.326689360501403E-5
5	0.0	0.0	0.0	0.0	6.326689360501403E-5
6	0.0	0.0	0.0	0.0	1.5816723401253506E-4
7	0.0	0.0	0.0	0.0	1.5816723401253506E-4
8	0.0	0.0	0.0	0.0	1.5816723401253506E-4
9	0.0	0.0	0.0	0.0	1.8980068081504207E-4
10-11	0.0	0.0	0.0	1.5816723401253507E-5	1.8980068081504207E-4
12-13	0.0	0.0	0.0	3.1633446802507014E-5	2.6888429782130966E-4
14-15	0.0	0.0	0.0	3.1633446802507014E-5	3.321511914263236E-4
16-17	0.0	0.0	0.0	3.1633446802507014E-5	5.061351488401122E-4
18-19	0.0	0.0	0.0	3.1633446802507014E-5	5.377685956426192E-4
20-21	0.0	0.0	0.0	6.326689360501403E-5	5.377685956426192E-4
22-23	0.0	0.0	0.0	3.163344680250701E-4	6.484856594513937E-4
24-25	0.0	0.0	0.0	5.852187658463798E-4	7.275692764576613E-4
26-27	0.0	0.0	0.0	0.001091353914686492	7.433859998589148E-4
28-29	0.0	0.0	0.0	0.004270515318338447	7.592027232601683E-4
30-31	0.0	0.0	0.0	0.019359669443134293	7.592027232601683E-4
32-33	0.0	0.0	0.0	0.057509606286957754	7.908361700626754E-4
34-35	0.0	0.0	0.0	0.11108084844700339	8.224696168651823E-4
36-37	0.0	0.0	0.0	0.1975508752816563	8.541030636676893E-4
38-39	0.0	0.0	0.0	0.3380666459783924	8.541030636676893E-4
40-41	0.0	0.0	0.0	0.49496854211882724	8.541030636676893E-4
42-43	0.0	0.0	0.0	0.6585292788111897	8.699197870689428E-4
44-45	0.0	0.0	0.0	0.8631660461766076	8.857365104701964E-4
46-47	0.0	0.0	0.0	1.1007964985570404	9.64820127476464E-4
48-49	1.5816723401253507E-5	0.0	0.0	1.3372406966823789	0.0011388040848902526
50-51	3.1633446802507014E-5	0.0	0.0	1.5871291096987832	0.0011388040848902526
52-53	3.1633446802507014E-5	0.0	0.0	1.8397538158636042	0.001249521148699027
54-55	3.1633446802507014E-5	0.0	0.0	2.141094030104286	0.0013602382125078017
56-57	3.1633446802507014E-5	0.0	0.0	2.4876226231023493	0.0013918716593103086
58-59	3.1633446802507014E-5	0.0	0.0	2.842059577801039	0.0013918716593103086
60-61	3.1633446802507014E-5	0.0	0.0	3.214954648708992	0.0014235051061128156
62-63	3.1633446802507014E-5	0.0	0.0	3.5819500817882766	0.0014235051061128156
64-65	3.1633446802507014E-5	0.0	0.0	3.987949554775053	0.0014867719997178296
66-67	3.1633446802507014E-5	0.0	0.0	4.469220814428395	0.0014867719997178296
68-69	3.1633446802507014E-5	0.0	0.0	4.976463133906595	0.0015342221699215903
70-71	3.1633446802507014E-5	1.5816723401253507E-5	0.0	5.495156761127302	0.0016449392337303647
72-73	3.1633446802507014E-5	3.1633446802507014E-5	0.0	6.036357585747993	0.0018505566379466603
74-75	3.1633446802507014E-5	3.1633446802507014E-5	0.0	6.604858074959248	0.0019138235315516745
76-77	3.1633446802507014E-5	3.1633446802507014E-5	0.0	7.2433475652210495	0.001929640254952928
78-79	3.1633446802507014E-5	3.1633446802507014E-5	0.0	7.940849250492928	0.001929640254952928
80-81	3.1633446802507014E-5	3.1633446802507014E-5	0.0	8.651099214826218	0.001992907148557942
82-83	3.1633446802507014E-5	3.1633446802507014E-5	0.0	9.38703553796314	0.002151074382570477
84-85	3.1633446802507014E-5	3.1633446802507014E-5	0.0	10.154858375476993	0.002151074382570477
86-87	3.1633446802507014E-5	6.326689360501403E-5	0.0	10.977929027831422	0.002245974722977998
88-89	3.1633446802507014E-5	7.908361700626753E-5	0.0	11.855140324388344	0.0023566917867867726
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2160	0.0	54.268387	1
GTATCAA	4180	0.0	43.029537	1
TCAACGC	4820	0.0	35.939915	4
CAACGCA	4945	0.0	35.22115	5
ATCAACG	5140	0.0	33.79475	3
TATCAAC	5305	0.0	32.833103	2
AACGCAG	5415	0.0	32.232853	6
GTACATG	8010	0.0	28.616621	1
ACGCAGA	6255	0.0	27.228676	7
TACATGG	8500	0.0	26.186962	2
CGCAGAG	6735	0.0	24.725754	8
ACATGGG	8875	0.0	24.49223	3
CATGGGG	7070	0.0	22.622559	4
GCAGAGT	7905	0.0	20.046171	9
CAGAGTA	7060	0.0	18.245775	10-11
GAGTACA	6060	0.0	18.090189	1
TAATACT	2310	0.0	18.080147	4
AGAGTAC	6350	0.0	17.894888	10-11
ATGGGGG	4105	0.0	17.225698	5
TTAATAC	2260	0.0	16.800135	3
>>END_MODULE