FastQCFastQC Report
Mon 15 Aug 2016
SRR2921651_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921651_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3161211
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23000.046.3041731
GTATCAA42000.043.2428251
TCAACGC53300.033.3043984
CAACGCA56100.031.5815285
ATCAACG57400.030.9250263
TATCAAC59050.030.2216572
AACGCAG59750.029.728016
GTACATG81900.027.5165791
TACATGG86500.025.8985962
ACATGGG87850.024.5821593
ACGCAGA71500.024.576947
CGCAGAG74350.023.2515858
CATGGGG73850.021.5946434
CCCTATA4400.020.495162
ATGGGGG38300.019.346975
TAAGGTG16200.019.058365
GCAGAGT86250.019.052989
AGAGTAC71800.018.52164310-11
GTAAGGT13100.017.3911044
GTATATA9350.016.7803971
Produced by FastQC (version 0.11.5)