##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921651_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3161211 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.404844852178485 33.0 31.0 34.0 29.0 34.0 2 31.543152608288406 34.0 31.0 34.0 28.0 34.0 3 31.616914530539088 34.0 31.0 34.0 28.0 34.0 4 35.09437649052847 37.0 35.0 37.0 32.0 37.0 5 34.840994795981665 37.0 35.0 37.0 32.0 37.0 6 34.814923458130444 37.0 35.0 37.0 32.0 37.0 7 34.69685762829498 37.0 35.0 37.0 32.0 37.0 8 34.64101352298217 37.0 35.0 37.0 32.0 37.0 9 36.23355827877354 39.0 37.0 39.0 32.0 39.0 10-11 36.130109473869354 39.0 37.0 39.0 32.0 39.0 12-13 36.054135741018236 39.0 37.0 39.0 31.5 39.0 14-15 37.303198046571396 40.0 38.0 41.0 31.0 41.0 16-17 37.26749827834966 40.0 38.0 41.0 31.0 41.0 18-19 37.229206623664155 40.0 38.0 41.0 31.0 41.0 20-21 37.16710099389127 40.0 38.0 41.0 31.0 41.0 22-23 37.07390095124938 40.0 38.0 41.0 31.0 41.0 24-25 36.99082029007238 40.0 37.0 41.0 30.5 41.0 26-27 36.8607195154009 40.0 37.0 41.0 30.0 41.0 28-29 36.73712542440223 40.0 37.0 41.0 30.0 41.0 30-31 36.58110531059141 40.0 37.0 41.0 30.0 41.0 32-33 36.524441582671955 40.0 37.0 41.0 30.0 41.0 34-35 36.6432871769711 40.0 37.0 41.0 30.0 41.0 36-37 36.629182455710804 40.0 37.0 41.0 30.0 41.0 38-39 36.51647786243942 40.0 37.0 41.0 30.0 41.0 40-41 36.42747573635547 40.0 37.0 41.0 30.0 41.0 42-43 36.29863681987694 40.0 36.0 41.0 29.0 41.0 44-45 36.15782780712834 40.0 36.0 41.0 28.5 41.0 46-47 35.99921027100058 40.0 36.0 41.0 28.0 41.0 48-49 35.84819757365136 40.0 35.5 41.0 28.0 41.0 50-51 35.68438044787267 39.5 35.0 41.0 27.0 41.0 52-53 35.50213937000726 39.0 35.0 41.0 27.0 41.0 54-55 35.28247924608639 39.0 35.0 41.0 26.5 41.0 56-57 35.0454275276152 39.0 35.0 41.0 26.0 41.0 58-59 34.79734997126101 38.5 35.0 40.5 26.0 41.0 60-61 34.515497383755786 38.0 34.0 40.0 26.0 41.0 62-63 34.20719638771344 37.0 34.0 40.0 25.0 41.0 64-65 33.863022904829826 37.0 34.0 40.0 24.0 41.0 66-67 33.5111462031481 36.0 34.0 39.0 23.0 41.0 68-69 33.147106599338045 36.0 33.5 39.0 22.5 41.0 70-71 32.747180431802875 35.0 33.0 38.5 22.0 40.0 72-73 32.38543124770855 35.0 33.0 37.5 21.0 39.5 74-75 31.981798905546007 35.0 33.0 37.0 20.0 39.0 76-77 29.277515641948607 33.0 29.0 34.5 13.0 36.5 78-79 30.849050569544392 34.5 31.5 36.0 16.0 37.0 80-81 31.03127092750215 35.0 32.0 35.5 18.0 37.0 82-83 30.859696331564074 35.0 32.0 35.0 16.0 37.0 84-85 30.66494628166231 35.0 32.0 35.0 13.5 36.0 86-87 30.455415345574846 35.0 32.0 35.0 8.5 36.0 88-89 30.18531505805845 35.0 31.5 35.0 2.0 36.0 90-91 29.985293926915983 34.5 31.0 35.0 2.0 35.0 92-93 29.82475687323624 34.0 31.0 35.0 2.0 35.0 94-95 29.716566214656346 34.0 31.0 35.0 2.0 35.0 96-97 29.50897077101149 34.0 31.0 35.0 2.0 35.0 98-99 29.29743490706568 34.0 31.0 35.0 2.0 35.0 100-101 28.17361748393258 33.0 28.5 34.5 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 57024.0 3 19896.0 4 10841.0 5 7590.0 6 6972.0 7 6960.0 8 7390.0 9 7893.0 10 8653.0 11 9305.0 12 10248.0 13 11070.0 14 11621.0 15 12498.0 16 13220.0 17 14272.0 18 13403.0 19 12465.0 20 12154.0 21 12043.0 22 12517.0 23 13968.0 24 16474.0 25 19758.0 26 23686.0 27 28778.0 28 34549.0 29 41676.0 30 50665.0 31 62607.0 32 79924.0 33 104908.0 34 148742.0 35 224472.0 36 394773.0 37 756236.0 38 785806.0 39 96152.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.575064416248736 19.234349671550007 12.898807237940252 26.291778674261003 2 15.490460931099168 20.82003410086644 37.23330623400533 26.45619873402906 3 18.09340527926927 24.258057535657656 29.00690691108712 28.641630273985953 4 13.145853290022307 16.40588520385244 35.14254295776163 35.30571854836362 5 14.229049409638417 37.10105188018362 34.37899074684632 14.290907963331643 6 34.1517825367486 35.39725054972239 16.528399081217827 13.92256783231118 7 29.232974325345573 31.55898166873391 20.87064102965604 18.337402976264475 8 26.66747015621545 34.75516186676562 19.825661747982025 18.75170622903691 9 26.682837700018474 15.257363640734809 19.217210635934112 38.84258802331261 10-11 25.702546742821987 25.038081351831813 27.982364053609178 21.277007851737025 12-13 26.102626818098564 22.617274257473664 27.570249408373588 23.70984951605418 14-15 23.345772230257523 24.299078281534122 24.621360230708007 27.73378925750035 16-17 23.660410519892537 27.31056231298702 25.586887430165213 23.442139736955237 18-19 23.668793947530588 26.589560907465497 26.799879537738725 22.94176560726519 20-21 24.127847571904613 25.947740764430744 26.64227088303753 23.282140780627117 22-23 24.038335308842086 25.72817822030861 26.383022202567307 23.850464268281996 24-25 23.83762881624604 25.905157837762943 26.383743585087764 23.873469760903255 26-27 23.643252742107816 26.073690712224394 26.429102999176717 23.853953546491073 28-29 23.794141573499704 26.178391170111446 26.31742784368355 23.710039412705296 30-31 24.01765127887929 25.949942694248307 26.086036933537194 23.94636909333521 32-33 23.727825299465728 26.081932399245456 25.96906674259591 24.22117555869291 34-35 24.117927973259476 26.037478935953434 26.076154684645537 23.768438406141552 36-37 24.24337232577764 25.804071864622323 26.088167581833122 23.864388227766913 38-39 23.97252192276947 25.949027129160314 26.1469576058036 23.93149334226662 40-41 24.148002277688146 25.818987061465947 26.17247160798456 23.860539052861345 42-43 24.077194956129667 25.81465567150684 25.930935624153328 24.177213748210168 44-45 23.921899065370887 25.821907179926267 26.146726662704822 24.10946709199802 46-47 24.125069378681776 25.833165010778707 25.907110941198034 24.13465466934148 48-49 23.81777635625476 25.913394448277703 25.969137554181067 24.29969164128647 50-51 23.81363401119349 26.024588089552957 26.03673566743981 24.125042231813747 52-53 24.107888943211776 25.802009054013592 25.986689293528485 24.103412709246143 54-55 23.633253349741306 25.715905873495164 26.190476943688285 24.460363833075245 56-57 23.611249518736912 25.888363414451277 26.122074576876024 24.378312489935787 58-59 23.68130978083419 25.617598186737673 26.15834660038624 24.542745432041894 60-61 23.54093838283818 25.7182130801621 26.272236054122626 24.46861248287709 62-63 23.628800721906977 25.720214218049065 26.2329149841893 24.418070075854654 64-65 23.425554710420172 25.636943657810995 26.108332994452855 24.82916863731598 66-67 23.39915959271377 25.758051265445857 25.974412381977473 24.8683767598629 68-69 23.583900043391417 25.65591143973103 26.005194943301564 24.754993573575987 70-71 23.42612848769243 25.522075621551004 25.998544509734344 25.053251381022218 72-73 23.479236198154574 25.640770864825885 25.84981230256011 25.03018063445943 74-75 23.408199466388556 25.826458571703405 25.762968808537785 25.002373153370254 76-77 23.336860050621127 25.545217070664446 25.996181380010707 25.121741498703713 78-79 23.3345195436594 25.665011248946502 25.904743933705614 25.095725273688487 80-81 23.4745361130517 25.78720473194917 25.66112535733832 25.077133797660807 82-83 23.314004116028464 25.762405041502024 25.792291179902843 25.13129966256667 84-85 23.258228906165808 25.725614983137785 25.74574425038023 25.270411860316177 86-87 23.17912030515503 25.927209129326855 25.695129864991056 25.198540700527055 88-89 23.066609346470447 25.714992774165303 25.687247472544207 25.531150406820046 90-91 22.95838528826659 25.715708950843496 25.75816128714976 25.56774447374015 92-93 23.067391517986216 25.535927726290925 25.820383286261674 25.576297469461185 94-95 22.855554246150415 25.47302700107644 25.881092128251755 25.7903266245214 96-97 22.795144609583627 25.384400366433812 25.896354154390377 25.924100869592177 98-99 22.648866973008165 25.404184769095572 25.791413833703764 26.1555344241925 100-101 22.580309948214374 25.379953944784674 25.82706185978735 26.212674247213602 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 72.0 1 56.5 2 80.5 3 137.5 4 137.0 5 121.0 6 191.0 7 434.5 8 635.0 9 641.5 10 523.0 11 421.5 12 418.5 13 453.0 14 503.0 15 533.5 16 577.0 17 650.5 18 752.0 19 841.5 20 933.5 21 1064.5 22 1210.5 23 1487.5 24 1808.5 25 2158.0 26 2841.5 27 3834.0 28 5162.0 29 7443.0 30 11111.0 31 16187.5 32 24155.0 33 31198.0 34 36843.0 35 44527.0 36 54392.0 37 63988.5 38 72158.0 39 80283.5 40 87156.5 41 93672.5 42 100553.0 43 108204.0 44 115665.5 45 123506.0 46 128796.0 47 132117.5 48 137366.5 49 139573.5 50 138696.0 51 138256.5 52 137164.5 53 135812.5 54 133285.5 55 125835.5 56 115932.5 57 104966.0 58 94803.5 59 83504.5 60 69208.0 61 57287.5 62 47687.5 63 36757.5 64 26189.0 65 19089.5 66 14302.5 67 11139.0 68 8623.5 69 6632.0 70 5101.0 71 3741.5 72 2691.5 73 2089.5 74 1631.5 75 1191.0 76 819.5 77 480.0 78 291.0 79 181.0 80 107.5 81 63.0 82 37.5 83 21.5 84 14.0 85 10.5 86 4.0 87 2.5 88 4.0 89 4.5 90 2.5 91 1.0 92 1.5 93 1.0 94 0.0 95 1.0 96 1.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1637030872029738 2 3.1633446802507014E-5 3 0.010091069529999736 4 0.006295055913698896 5 0.024515921271942936 6 0.0022143412761754908 7 0.0 8 0.0 9 9.490034040752104E-5 10-11 0.048335906714230714 12-13 3.163344680250702E-4 14-15 0.005029718041598615 16-17 0.0 18-19 0.0017398395741378856 20-21 2.847010212225631E-4 22-23 0.0 24-25 6.959358296551543E-4 26-27 0.0029577272760344057 28-29 0.0055200364670374745 30-31 0.01687644386913749 32-33 0.011815092380736369 34-35 0.009980352466190962 36-37 0.004064897914122152 38-39 0.0 40-41 0.0035113125950782783 42-43 0.009648201274764638 44-45 0.007101708807162825 46-47 0.0037169299992945745 48-49 0.008920631998306979 50-51 0.0027521098718181103 52-53 0.0022617914463792517 54-55 0.004618483233166024 56-57 0.00816142927504681 58-59 0.015785089954451 60-61 0.00762366067940419 62-63 0.02192197863413736 64-65 0.041012763779450345 66-67 0.06040406666938715 68-69 0.050613514884011224 70-71 0.06763230926376 72-73 0.03391105497228752 74-75 0.027173130803353526 76-77 0.22750774940363044 78-79 0.08518887223915139 80-81 0.06611390381723967 82-83 0.027932333526613694 84-85 0.0516416019050927 86-87 0.06835987854021766 88-89 0.2950135248801804 90-91 0.2986513712624687 92-93 0.05271713909637794 94-95 0.010075252806598484 96-97 0.015073337401394594 98-99 0.0010439037444827314 100-101 1.7398395741378858E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3161211.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.9319207681153 #Duplication Level Percentage of deduplicated Percentage of total 1 78.07236883071876 45.22882285280273 2 11.99282695989099 13.89535002452244 3 4.010076696190765 6.969343363133669 4 1.8287849001838454 4.237800877575047 5 1.0160601375364684 2.943115769170151 6 0.6367146899062923 2.213166298052786 7 0.43274288871992184 1.754873871960149 8 0.31819165764452195 1.4746763119790172 9 0.2475056821821001 1.2904631612848572 >10 1.3493257246558719 13.90198375672763 >50 0.06968707969152631 2.7189868455137356 >100 0.023526722924636662 2.1899170487523074 >500 0.0015316208288672897 0.6045030188545705 >1k 6.564089253573596E-4 0.5769967996708769 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 3.1633446802507014E-5 10-11 0.0 0.0 0.0 1.5816723401253507E-5 3.1633446802507014E-5 12-13 0.0 0.0 0.0 3.1633446802507014E-5 9.490034040752105E-5 14-15 0.0 0.0 0.0 6.326689360501403E-5 3.0051774462381664E-4 16-17 0.0 0.0 0.0 6.326689360501403E-5 3.7960136163008414E-4 18-19 0.0 0.0 0.0 6.326689360501403E-5 4.2705153183384467E-4 20-21 0.0 3.1633446802507014E-5 0.0 9.490034040752105E-5 6.168522126488868E-4 22-23 0.0 3.1633446802507014E-5 0.0 3.0051774462381664E-4 6.643023828526473E-4 24-25 0.0 3.1633446802507014E-5 0.0 5.852187658463798E-4 6.643023828526473E-4 26-27 0.0 3.1633446802507014E-5 0.0 0.0010755371912852384 6.801191062539008E-4 28-29 0.0 3.1633446802507014E-5 0.0 0.004286332041739701 7.117525530564078E-4 30-31 0.0 3.1633446802507014E-5 0.0 0.019549470123949336 7.433859998589148E-4 32-33 3.1633446802507014E-5 3.1633446802507014E-5 0.0 0.05836370935062544 7.908361700626754E-4 34-35 3.1633446802507014E-5 3.1633446802507014E-5 0.0 0.11199821840427607 7.908361700626754E-4 36-37 3.1633446802507014E-5 3.1633446802507014E-5 0.0 0.19925908140899168 8.066528934639289E-4 38-39 3.1633446802507014E-5 3.1633446802507014E-5 0.0 0.33996465278654286 8.857365104701964E-4 40-41 3.1633446802507014E-5 3.1633446802507014E-5 0.0 0.4970405328843915 9.490034040752104E-4 42-43 3.1633446802507014E-5 3.1633446802507014E-5 0.0 0.661107404725594 9.490034040752104E-4 44-45 3.1633446802507014E-5 3.1633446802507014E-5 0.0 0.8655860048569994 9.64820127476464E-4 46-47 3.1633446802507014E-5 3.1633446802507014E-5 0.0 1.1032322739608333 9.806368508777175E-4 48-49 3.1633446802507014E-5 3.1633446802507014E-5 0.0 1.3392968707245418 0.0010439037444827314 50-51 3.1633446802507014E-5 3.1633446802507014E-5 0.0 1.5889005827197236 0.0010439037444827314 52-53 3.1633446802507014E-5 3.1633446802507014E-5 0.0 1.8419997905865821 0.0012020709784952665 54-55 3.1633446802507014E-5 3.1633446802507014E-5 0.0 2.1429604034656338 0.001344421489106548 56-57 3.1633446802507014E-5 3.1633446802507014E-5 0.0 2.488112941527788 0.0013918716593103086 58-59 3.1633446802507014E-5 3.1633446802507014E-5 0.0 2.8424233624392676 0.0013918716593103086 60-61 3.1633446802507014E-5 3.1633446802507014E-5 0.0 3.213974011858114 0.0014076883827115621 62-63 3.1633446802507014E-5 3.1633446802507014E-5 0.0 3.5817286476606593 0.0014551385529153226 64-65 3.1633446802507014E-5 3.1633446802507014E-5 0.0 3.987475053073015 0.0014551385529153226 66-67 3.1633446802507014E-5 3.1633446802507014E-5 0.0 4.467844759492486 0.001502588723119083 68-69 3.1633446802507014E-5 3.1633446802507014E-5 0.0 4.973742657481579 0.0015184054465203366 70-71 3.1633446802507014E-5 3.1633446802507014E-5 0.0 5.493053136914936 0.0015342221699215903 72-73 3.1633446802507014E-5 3.1633446802507014E-5 0.0 6.034475395663244 0.0016291225103291112 74-75 3.1633446802507014E-5 3.1633446802507014E-5 0.0 6.604573373938026 0.0016765726805328717 76-77 3.1633446802507014E-5 3.1633446802507014E-5 0.0 7.242841430072209 0.0017082061273353787 78-79 3.1633446802507014E-5 3.1633446802507014E-5 0.0 7.941513552875781 0.0017398395741378859 80-81 3.1633446802507014E-5 3.1633446802507014E-5 0.0 8.655005945506327 0.0018031064677428998 82-83 3.1633446802507014E-5 3.1633446802507014E-5 0.0 9.39242904064297 0.0020719907655642094 84-85 3.1633446802507014E-5 3.1633446802507014E-5 0.0 10.163051438198842 0.002087807488965463 86-87 3.1633446802507014E-5 3.1633446802507014E-5 0.0 10.98887420042509 0.0021036242123667163 88-89 3.1633446802507014E-5 3.1633446802507014E-5 0.0 11.871352465874628 0.00211944093576797 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2300 0.0 46.304173 1 GTATCAA 4200 0.0 43.242825 1 TCAACGC 5330 0.0 33.304398 4 CAACGCA 5610 0.0 31.581528 5 ATCAACG 5740 0.0 30.925026 3 TATCAAC 5905 0.0 30.221657 2 AACGCAG 5975 0.0 29.72801 6 GTACATG 8190 0.0 27.516579 1 TACATGG 8650 0.0 25.898596 2 ACATGGG 8785 0.0 24.582159 3 ACGCAGA 7150 0.0 24.57694 7 CGCAGAG 7435 0.0 23.251585 8 CATGGGG 7385 0.0 21.594643 4 CCCTATA 440 0.0 20.49516 2 ATGGGGG 3830 0.0 19.34697 5 TAAGGTG 1620 0.0 19.05836 5 GCAGAGT 8625 0.0 19.05298 9 AGAGTAC 7180 0.0 18.521643 10-11 GTAAGGT 1310 0.0 17.391104 4 GTATATA 935 0.0 16.780397 1 >>END_MODULE