FastQCFastQC Report
Mon 15 Aug 2016
SRR2921650_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921650_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3332077
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24650.050.830851
GTATCAA45500.040.0553171
TCAACGC52000.033.5915834
CAACGCA52750.033.1999745
ATCAACG55250.031.613713
TATCAAC56150.031.0233922
AACGCAG57850.030.1733826
GTACATG88600.030.1588671
TACATGG92050.027.7415962
ACATGGG97200.025.9291423
ACGCAGA68300.025.0766987
CATGGGG80600.024.2632084
CGCAGAG73450.022.9964928
ATGGGGG51500.020.6430875
TAAGGCG10950.020.3712445
GAGTACA66300.019.6145081
GCAGAGT83450.019.1607919
TAAGGTG16350.018.8681555
AGAGTAC68900.017.4225310-11
AGTACAT69200.017.4221042
Produced by FastQC (version 0.11.5)