##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921650_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3332077
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.438635721803546	31.0	31.0	34.0	26.0	34.0
2	30.478476337731692	31.0	31.0	34.0	26.0	34.0
3	30.542018086616846	33.0	31.0	34.0	26.0	34.0
4	34.002559364624524	37.0	35.0	37.0	30.0	37.0
5	34.001585497574034	37.0	35.0	37.0	30.0	37.0
6	34.04717027847796	37.0	35.0	37.0	30.0	37.0
7	33.95640106756237	37.0	35.0	37.0	30.0	37.0
8	33.926080639793135	37.0	35.0	37.0	30.0	37.0
9	35.488616259468195	39.0	37.0	39.0	30.0	39.0
10-11	35.391505658482686	39.0	36.0	39.0	30.0	39.0
12-13	35.27985757832127	39.0	35.0	39.0	29.0	39.0
14-15	36.424263604952706	40.0	37.0	41.0	29.0	41.0
16-17	36.36558804013232	40.0	37.0	41.0	28.5	41.0
18-19	36.32690991234597	40.0	37.0	41.0	28.0	41.0
20-21	36.24592723997675	40.0	37.0	41.0	27.0	41.0
22-23	36.14394925447401	40.0	36.5	41.0	27.0	41.0
24-25	36.03257412718854	40.0	36.0	41.0	27.0	41.0
26-27	35.88403884424039	40.0	36.0	41.0	27.0	41.0
28-29	35.7409905293305	40.0	36.0	41.0	27.0	41.0
30-31	35.59015037767735	40.0	36.0	41.0	26.0	41.0
32-33	35.41027788373438	39.5	35.0	41.0	25.0	41.0
34-35	35.236720519963974	39.0	35.0	41.0	25.0	41.0
36-37	35.202225068628366	39.0	35.0	41.0	24.5	41.0
38-39	35.382130575013726	39.5	35.0	41.0	25.0	41.0
40-41	35.443582486239066	40.0	35.0	41.0	25.0	41.0
42-43	35.27631219206519	40.0	35.0	41.0	24.0	41.0
44-45	35.150332060153474	40.0	35.0	41.0	24.0	41.0
46-47	34.98963064178889	40.0	35.0	41.0	23.0	41.0
48-49	34.820801410051445	39.0	35.0	41.0	22.5	41.0
50-51	33.1007761825432	37.0	32.5	39.5	18.5	40.0
52-53	33.21636624843904	37.5	32.5	39.5	19.0	40.0
54-55	34.12825423902269	38.5	34.0	40.5	20.0	41.0
56-57	34.14581550786491	38.5	34.0	41.0	20.0	41.0
58-59	33.94753422564965	38.0	34.0	41.0	18.5	41.0
60-61	33.68754443549774	38.0	34.0	40.0	18.0	41.0
62-63	33.3884793178549	37.0	33.5	40.0	15.0	41.0
64-65	33.09029968395088	37.0	33.0	40.0	13.0	41.0
66-67	32.747561355875035	36.0	33.0	39.0	10.0	41.0
68-69	32.38220290227386	35.5	33.0	39.0	7.5	41.0
70-71	31.96791670780717	35.0	32.0	38.5	4.5	40.5
72-73	31.604145852571833	35.0	32.0	37.5	2.0	40.0
74-75	31.20249216929861	35.0	32.0	37.0	2.0	39.0
76-77	30.822568175945513	35.0	32.0	36.5	2.0	39.0
78-79	30.470771833904198	35.0	31.0	36.0	2.0	38.0
80-81	30.146505017741184	35.0	31.0	35.5	2.0	37.0
82-83	29.823607317598004	35.0	31.0	35.0	2.0	37.0
84-85	29.54887372050526	35.0	31.0	35.0	2.0	36.0
86-87	29.29358865356353	35.0	30.5	35.0	2.0	36.0
88-89	29.043773448212633	35.0	30.0	35.0	2.0	36.0
90-91	28.795228471610947	34.0	30.0	35.0	2.0	35.0
92-93	28.577246264116944	34.0	30.0	35.0	2.0	35.0
94-95	28.311562577935625	34.0	29.5	35.0	2.0	35.0
96-97	28.034659163038548	34.0	29.0	35.0	2.0	35.0
98-99	27.724888560498453	34.0	29.0	35.0	2.0	35.0
100-101	26.60625309679218	33.0	26.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	110427.0
3	19452.0
4	13363.0
5	10244.0
6	9492.0
7	9539.0
8	10256.0
9	11086.0
10	11913.0
11	12604.0
12	12797.0
13	12846.0
14	13662.0
15	14583.0
16	14617.0
17	15142.0
18	14621.0
19	13959.0
20	14642.0
21	15157.0
22	16981.0
23	19258.0
24	22657.0
25	26558.0
26	31295.0
27	36988.0
28	44173.0
29	52248.0
30	61987.0
31	74666.0
32	92754.0
33	120094.0
34	165736.0
35	240867.0
36	401351.0
37	710244.0
38	729037.0
39	124772.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.03061611784263	17.445091572099816	11.994065349617088	25.530226960440466
2	16.673724652071353	19.517462056570803	38.19799721404797	25.610816077309867
3	19.62500172687089	22.941044327424333	29.16853118668766	28.265422759017117
4	13.677852171620128	14.927474512917879	35.666027775258385	35.72864554020361
5	14.544308990211618	36.717693745033586	34.15344641496084	14.584550849793956
6	34.5607192873878	34.341674715509185	16.660473710149226	14.43713228695379
7	29.58058468537098	31.279989002406623	20.647111120715998	18.4923151915064
8	27.09209937835996	34.0794414544756	19.909740628123586	18.918718539040853
9	27.12144140423583	14.989576399022528	18.988631581980286	38.90035061476135
10-11	26.048563895628956	24.61867265848894	27.787295912058042	21.545467533824063
12-13	26.454191184107597	22.148922553404045	27.438641107891147	23.95824515459721
14-15	23.60717723374347	23.864731529257337	24.498627037601697	28.02946419939749
16-17	23.914289282302796	26.839136979006252	25.50303465856864	23.74353908012231
18-19	23.910495524265972	26.318528674471985	26.636245161693097	23.134730639568946
20-21	24.377579520019978	25.62694559164822	26.506047965583335	23.489426922748464
22-23	24.3444077069979	25.353026245908318	26.267269276876753	24.03529677021703
24-25	24.149823143760017	25.493747436920472	26.243247430015543	24.113181989303964
26-27	23.855966017691465	25.726992158355973	26.312485169436517	24.10455665451605
28-29	23.979732262728415	26.001958760380216	26.089766812668252	23.928542164223117
30-31	24.29441901355079	25.706826601658562	25.90591493138461	24.092839453406036
32-33	24.01629755799252	25.785332948154704	25.831374299831545	24.366995194021225
34-35	24.346125010729594	25.78650178006222	25.910334805363654	23.95703840384453
36-37	24.419794294865234	25.56718069441314	26.018944487944513	23.99408052277711
38-39	24.144130165355296	25.72727383227792	26.02882429234683	24.099771710019954
40-41	24.385108604165357	25.482442167788633	26.13264610493628	23.999803123109732
42-43	24.20244845167015	25.51692710291618	25.87941225128213	24.401212194131535
44-45	23.99000761532176	25.582248857889677	26.112765399225534	24.31497812756303
46-47	24.167110101764784	25.548251928472805	25.98282083061872	24.301817139143694
48-49	23.935442746556888	25.650409260891355	25.984882466719966	24.429265525831788
50-51	23.9946327688288	25.713166180729964	26.10169501719285	24.190506033248386
52-53	24.231724255738392	25.454685982434643	26.089293799375028	24.224295962451933
54-55	23.686020035368564	25.376995759291088	26.3908083239569	24.546175881383448
56-57	23.747776439506374	25.539345931128643	26.228300079544447	24.484577549820536
58-59	23.86108055011005	25.37043642713592	26.229705172725964	24.538777850028062
60-61	23.79783503605792	25.352657463988326	26.436447815688567	24.41305968426519
62-63	23.85868335324425	25.395435349912148	26.34228785849991	24.403593438343695
64-65	23.630941139250403	25.26589346097262	26.32443001993261	24.778735379844363
66-67	23.674838179624526	25.33583516876112	26.20658576750553	24.782740884108826
68-69	23.776281605323312	25.327215261339937	26.262863490443472	24.633639642893282
70-71	23.63449008810556	25.154112943442584	26.24177003129993	24.96962693715193
72-73	23.693900445652403	25.325353019284137	26.138726169986043	24.842020365077417
74-75	23.70672201877832	25.512005779794926	26.143227347872532	24.63804485355422
76-77	23.769269663352123	25.22120467711582	26.173665386044277	24.83586027348778
78-79	23.71131400647704	25.319806819658247	26.235888619947932	24.732990553916782
80-81	23.864943760405342	25.415393962909995	26.098254985915663	24.621407290769007
82-83	23.78184509938479	25.405861136063773	26.140634961169347	24.671658803382094
84-85	23.870760115194035	25.455911213372133	26.07239478769559	24.60093388373824
86-87	23.825653666388806	25.5411885707616	26.00466697210255	24.628490790747037
88-89	23.686331980921853	25.291643805618524	26.106399529303047	24.91562468415657
90-91	23.729014701741637	25.27794389801915	26.16312579868072	24.829915601558493
92-93	23.97549246615632	25.137338237928407	26.189614228640025	24.697555067275246
94-95	23.920522426279426	25.037192504734513	26.195706501164096	24.846578567821968
96-97	24.008350071231018	24.858714995757424	26.21441325903226	24.918521673979296
98-99	23.86770734219528	24.91006244714975	26.119628150822543	25.102602059832424
100-101	24.159769221767256	24.808539685089638	26.2159914826972	24.81569961044591
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	74.0
1	67.0
2	110.0
3	176.5
4	172.5
5	180.0
6	279.0
7	496.5
8	761.0
9	821.0
10	696.5
11	622.5
12	715.5
13	816.5
14	882.5
15	1014.0
16	1106.5
17	1213.0
18	1351.5
19	1480.5
20	1642.0
21	1784.5
22	1946.0
23	2145.5
24	2385.5
25	2699.0
26	3126.0
27	3868.5
28	5088.5
29	7131.5
30	10468.5
31	15582.5
32	23242.0
33	29807.0
34	35126.5
35	42337.0
36	51807.5
37	61513.5
38	70449.0
39	78933.5
40	86406.0
41	93791.5
42	101732.0
43	110579.0
44	119085.0
45	128205.5
46	135500.0
47	141259.0
48	147563.5
49	150103.5
50	150118.0
51	150679.0
52	149209.5
53	148097.5
54	145438.5
55	136857.0
56	125115.5
57	112044.5
58	101479.0
59	89066.5
60	73317.5
61	61016.0
62	51113.5
63	39671.5
64	28774.0
65	21306.5
66	16301.5
67	12876.0
68	9994.0
69	7742.0
70	6057.5
71	4423.0
72	3201.0
73	2463.0
74	1920.5
75	1500.5
76	1124.0
77	754.0
78	480.5
79	343.0
80	251.5
81	189.0
82	138.5
83	107.0
84	94.0
85	74.0
86	63.0
87	61.5
88	48.5
89	43.0
90	40.5
91	31.5
92	26.0
93	21.5
94	18.0
95	17.5
96	19.0
97	15.0
98	10.5
99	9.0
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05417041683010327
2	0.07547844782698598
3	0.07067663802487158
4	0.07055659277981871
5	0.0663550092029686
6	0.010053789273177061
7	0.013174965644551432
8	0.017016413486242964
9	0.0064524319215912476
10-11	0.012754807286866421
12-13	0.023258766228991708
14-15	0.03970496480123359
16-17	0.026860123580577516
18-19	0.00999376665065063
20-21	0.020032550268196083
22-23	0.03703395809880744
24-25	0.03370270254859056
26-27	0.02090287829482932
28-29	0.011344275657495309
30-31	0.011704411392653892
32-33	0.008688274610700774
34-35	0.005131934226009782
36-37	7.802940928435928E-4
38-39	0.003916476119849572
40-41	0.0013655146624762873
42-43	0.22589513987822013
44-45	0.19640902656211126
46-47	0.2014509268543314
48-49	0.2100041505643477
50-51	0.2014509268543314
52-53	0.20865364155750302
54-55	0.20430200142433683
56-57	0.2068379572260785
58-59	0.216531610764097
60-61	0.22077821130784192
62-63	0.20556247649739187
64-65	0.20626774231207742
66-67	0.2036267469209145
68-69	0.19892997670822132
70-71	0.19058683217704753
72-73	0.20190109652327964
74-75	0.2010607798079096
76-77	0.1944883026412655
78-79	0.21311032128009047
80-81	0.22394440464611112
82-83	0.22353925194405772
84-85	0.21764202928083598
86-87	0.23606897439645003
88-89	0.22943647460727948
90-91	0.23543873685992253
92-93	0.23143222680628328
94-95	0.2346434371114473
96-97	0.24109586903303856
98-99	0.23486852194592145
100-101	0.24069071633098515
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3332077.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.72313169602326
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.45862101860244	46.07335934763769
2	11.71025569386159	13.753257746094807
3	3.8703834199443663	6.818431058504936
4	1.8077860305809574	4.246354286081467
5	1.028607759558224	3.0201534464044504
6	0.651748513348528	2.296362826923179
7	0.45371675148642465	1.8650567985160418
8	0.32918075758119847	1.5464419983389939
9	0.25044998488785125	1.323648670125269
>10	1.3602798632072493	13.9769978397535
>50	0.06096333557954967	2.4329252552243954
>100	0.016011336326389406	1.5422354054237666
>500	0.0013303566942470175	0.5202371622090994
>1k	6.651783409982228E-4	0.5845381587623212
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.0011311263215107E-5
2	0.0	0.0	0.0	0.0	6.0022622526430215E-5
3	0.0	0.0	0.0	0.0	6.0022622526430215E-5
4	0.0	0.0	0.0	3.0011311263215107E-5	9.003393378964532E-5
5	0.0	0.0	0.0	3.0011311263215107E-5	9.003393378964532E-5
6	3.0011311263215107E-5	0.0	0.0	3.0011311263215107E-5	1.2004524505286043E-4
7	3.0011311263215107E-5	0.0	0.0	6.0022622526430215E-5	1.2004524505286043E-4
8	3.0011311263215107E-5	0.0	0.0	6.0022622526430215E-5	1.2004524505286043E-4
9	3.0011311263215107E-5	0.0	0.0	6.0022622526430215E-5	1.2004524505286043E-4
10-11	3.0011311263215107E-5	0.0	0.0	9.003393378964532E-5	1.2004524505286043E-4
12-13	3.0011311263215107E-5	0.0	0.0	9.003393378964532E-5	2.550961457373284E-4
14-15	3.0011311263215107E-5	0.0	0.0	1.0503958942125288E-4	3.7514139079018884E-4
16-17	3.0011311263215107E-5	0.0	0.0	1.2004524505286043E-4	4.501696689482266E-4
18-19	3.0011311263215107E-5	0.0	0.0	1.2004524505286043E-4	4.801809802114417E-4
20-21	3.0011311263215107E-5	0.0	0.0	1.2004524505286043E-4	5.101922914746568E-4
22-23	4.501696689482266E-5	0.0	0.0	1.8006786757929064E-4	5.852205696326945E-4
24-25	6.0022622526430215E-5	0.0	0.0	5.402036027378719E-4	6.7525450342234E-4
26-27	6.0022622526430215E-5	0.0	0.0	0.0012004524505286043	7.202714703171626E-4
28-29	6.0022622526430215E-5	0.0	0.0	0.006662511100433753	7.202714703171626E-4
30-31	6.0022622526430215E-5	0.0	0.0	0.028645796600738817	7.352771259487702E-4
32-33	6.0022622526430215E-5	0.0	0.0	0.07832952239699142	8.103054041068079E-4
34-35	6.0022622526430215E-5	0.0	0.0	0.1471754704348069	9.003393378964532E-4
36-37	6.0022622526430215E-5	1.5005655631607554E-5	0.0	0.25638663212164664	9.003393378964532E-4
38-39	6.0022622526430215E-5	3.0011311263215107E-5	0.0	0.4268658857523401	0.001005378927317706
40-41	6.0022622526430215E-5	3.0011311263215107E-5	0.0	0.6124858459153255	0.0010503958942125287
42-43	6.0022622526430215E-5	3.0011311263215107E-5	0.0	0.8043631644766913	0.0010654015498441363
44-45	6.0022622526430215E-5	3.0011311263215107E-5	0.0	1.0463593728476264	0.0011104185167389589
46-47	6.0022622526430215E-5	3.0011311263215107E-5	0.0	1.3248193244033677	0.001140429828002174
48-49	6.0022622526430215E-5	3.0011311263215107E-5	0.0	1.6046748019328485	0.0012604750730550345
50-51	6.0022622526430215E-5	3.0011311263215107E-5	0.0	1.8929934692385562	0.0012904863843182495
52-53	6.0022622526430215E-5	3.0011311263215107E-5	0.0	2.187179347896222	0.0013204976955814647
54-55	6.0022622526430215E-5	3.0011311263215107E-5	0.0	2.5284529739258725	0.001380520318107895
56-57	6.0022622526430215E-5	3.0011311263215107E-5	0.0	2.9225014908118867	0.00141053162937111
58-59	6.0022622526430215E-5	3.0011311263215107E-5	0.0	3.3285995491700824	0.0014405429406343252
60-61	6.0022622526430215E-5	3.0011311263215107E-5	0.0	3.7468221772786166	0.0014405429406343252
62-63	6.0022622526430215E-5	3.0011311263215107E-5	0.0	4.174093215733009	0.0015005655631607554
64-65	6.0022622526430215E-5	3.0011311263215107E-5	0.0	4.641999569637797	0.0015005655631607554
66-67	6.0022622526430215E-5	3.0011311263215107E-5	0.0	5.182068121474984	0.0015005655631607554
68-69	6.0022622526430215E-5	3.0011311263215107E-5	0.0	5.755944415450184	0.0015005655631607554
70-71	6.0022622526430215E-5	3.0011311263215107E-5	0.0	6.343220759904408	0.0015305768744239704
72-73	6.0022622526430215E-5	3.0011311263215107E-5	0.0	6.951715101421726	0.0015905994969504008
74-75	6.0022622526430215E-5	3.0011311263215107E-5	0.0	7.589020301751731	0.0016206108082136158
76-77	6.0022622526430215E-5	3.0011311263215107E-5	0.0	8.295681642411024	0.0016506221194768308
78-79	6.0022622526430215E-5	4.501696689482266E-5	0.0	9.070618716194133	0.0016506221194768308
80-81	6.0022622526430215E-5	6.0022622526430215E-5	0.0	9.847446502586825	0.0016506221194768308
82-83	6.0022622526430215E-5	9.003393378964532E-5	0.0	10.65054919199046	0.0016506221194768308
84-85	6.0022622526430215E-5	9.003393378964532E-5	0.0	11.483303056922153	0.0016656277751084384
86-87	6.0022622526430215E-5	9.003393378964532E-5	0.0	12.380116065745181	0.0017706673645296912
88-89	6.0022622526430215E-5	9.003393378964532E-5	0.0	13.340117890432904	0.0018607012983193367
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2465	0.0	50.83085	1
GTATCAA	4550	0.0	40.055317	1
TCAACGC	5200	0.0	33.591583	4
CAACGCA	5275	0.0	33.199974	5
ATCAACG	5525	0.0	31.61371	3
TATCAAC	5615	0.0	31.023392	2
AACGCAG	5785	0.0	30.173382	6
GTACATG	8860	0.0	30.158867	1
TACATGG	9205	0.0	27.741596	2
ACATGGG	9720	0.0	25.929142	3
ACGCAGA	6830	0.0	25.076698	7
CATGGGG	8060	0.0	24.263208	4
CGCAGAG	7345	0.0	22.996492	8
ATGGGGG	5150	0.0	20.643087	5
TAAGGCG	1095	0.0	20.371244	5
GAGTACA	6630	0.0	19.614508	1
GCAGAGT	8345	0.0	19.160791	9
TAAGGTG	1635	0.0	18.868155	5
AGAGTAC	6890	0.0	17.42253	10-11
AGTACAT	6920	0.0	17.422104	2
>>END_MODULE