FastQCFastQC Report
Mon 15 Aug 2016
SRR2921650_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921650_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3332077
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24450.044.521841
GTATCAA43700.039.050761
TCAACGC52300.032.0330244
ATCAACG53950.031.0537933
CAACGCA54250.030.90485
TATCAAC56750.029.6888852
AACGCAG58550.028.7123416
GTACATG83750.027.8117121
GTATACG1501.0542699E-625.3521731
TACATGG90700.024.959882
ACATGGG94250.023.3651223
ACGCAGA71900.023.1823487
CGCAGAG75600.021.7965058
CATGGGG78400.021.2478984
TAAGGCG11500.019.4111375
GAGTACA66050.018.9997351
GCAGAGT86000.018.1120519
AGAGTAC70500.017.81676310-11
ATGGGGG47150.017.6281725
GTATTAA21250.017.2245641
Produced by FastQC (version 0.11.5)