##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921650_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3332077
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.276737902515457	33.0	31.0	34.0	28.0	34.0
2	31.40452846677913	34.0	31.0	34.0	28.0	34.0
3	31.482613396989326	34.0	31.0	34.0	28.0	34.0
4	34.95899194406372	37.0	35.0	37.0	32.0	37.0
5	34.68684697262398	37.0	35.0	37.0	32.0	37.0
6	34.65566642067395	37.0	35.0	37.0	32.0	37.0
7	34.53609355366037	37.0	35.0	37.0	31.0	37.0
8	34.47806218163626	37.0	35.0	37.0	31.0	37.0
9	36.05933926496897	39.0	37.0	39.0	31.0	39.0
10-11	35.952748991094744	39.0	37.0	39.0	31.0	39.0
12-13	35.86715358018437	39.0	37.0	39.0	30.0	39.0
14-15	37.09352710036413	40.0	38.0	41.0	31.0	41.0
16-17	37.0521272467593	40.0	38.0	41.0	31.0	41.0
18-19	37.006179479045656	40.0	38.0	41.0	31.0	41.0
20-21	36.942329063824154	40.0	37.5	41.0	30.0	41.0
22-23	36.849319358466204	40.0	37.0	41.0	30.0	41.0
24-25	36.75699541157062	40.0	37.0	41.0	30.0	41.0
26-27	36.624194308835	40.0	37.0	41.0	30.0	41.0
28-29	36.49958239260377	40.0	37.0	41.0	30.0	41.0
30-31	36.33896740681563	40.0	36.5	41.0	29.5	41.0
32-33	36.281958520166256	40.0	36.5	41.0	29.5	41.0
34-35	36.4074157049792	40.0	37.0	41.0	30.0	41.0
36-37	36.39484126567304	40.0	37.0	41.0	30.0	41.0
38-39	36.285457839059546	40.0	37.0	41.0	29.0	41.0
40-41	36.19695733321889	40.0	36.0	41.0	28.5	41.0
42-43	36.065585219069064	40.0	36.0	41.0	28.0	41.0
44-45	35.92771025399473	40.0	36.0	41.0	28.0	41.0
46-47	35.765058100398036	40.0	35.0	41.0	27.0	41.0
48-49	35.61679201891193	40.0	35.0	41.0	27.0	41.0
50-51	35.44753497593243	39.0	35.0	41.0	26.0	41.0
52-53	35.26195523092653	39.0	35.0	41.0	26.0	41.0
54-55	35.03824881597875	39.0	35.0	41.0	26.0	41.0
56-57	34.80557997309186	39.0	35.0	41.0	25.0	41.0
58-59	34.55242015715723	38.0	34.5	40.0	24.5	41.0
60-61	34.263165437053225	38.0	34.0	40.0	23.5	41.0
62-63	33.953032898099295	37.0	34.0	40.0	23.0	41.0
64-65	33.61095736983269	37.0	34.0	40.0	22.0	41.0
66-67	33.2549399368622	36.0	33.5	39.0	22.0	41.0
68-69	32.89092538977941	35.5	33.0	39.0	21.0	40.5
70-71	32.49276202200609	35.0	33.0	38.5	20.0	40.0
72-73	32.13508346295718	35.0	33.0	37.0	19.5	39.5
74-75	31.737078104737677	35.0	32.5	37.0	17.5	39.0
76-77	29.044817691788033	33.0	28.5	34.5	9.5	36.5
78-79	30.614659415133563	34.5	31.0	35.5	11.0	37.0
80-81	30.799233481099026	35.0	32.0	35.0	10.5	37.0
82-83	30.6322553470403	35.0	32.0	35.0	7.5	37.0
84-85	30.44168201995332	35.0	32.0	35.0	7.0	36.0
86-87	30.240560317183547	35.0	32.0	35.0	2.0	36.0
88-89	29.973152781283268	35.0	31.0	35.0	2.0	35.5
90-91	29.775560108604935	34.0	31.0	35.0	2.0	35.0
92-93	29.615974510793116	34.0	31.0	35.0	2.0	35.0
94-95	29.5051756006839	34.0	31.0	35.0	2.0	35.0
96-97	29.298152923836994	34.0	31.0	35.0	2.0	35.0
98-99	29.08781339686928	34.0	31.0	35.0	2.0	35.0
100-101	27.940519231698428	33.0	28.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	69730.0
3	22931.0
4	12869.0
5	9079.0
6	7919.0
7	7645.0
8	8329.0
9	8883.0
10	9700.0
11	10351.0
12	10965.0
13	11613.0
14	12370.0
15	13405.0
16	14322.0
17	14849.0
18	14374.0
19	13277.0
20	13079.0
21	12917.0
22	13717.0
23	15043.0
24	18029.0
25	21555.0
26	25472.0
27	30383.0
28	37017.0
29	44675.0
30	54465.0
31	67273.0
32	85193.0
33	112296.0
34	159798.0
35	239611.0
36	420844.0
37	793753.0
38	799602.0
39	94742.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.97273195946017	18.984670557520356	12.453056494534218	26.589540988485254
2	15.777859275380933	20.844412538999805	36.67104032690809	26.706687858711163
3	18.29515545832209	24.03959025478963	28.910544645137705	28.754709641750576
4	13.292099281065251	16.059700937070247	34.672259515215764	35.975940266648735
5	14.26875369043041	37.13286690195365	34.1894081232546	14.408971284361341
6	34.61342690687504	34.875942228021884	16.37715051278573	14.13348035231735
7	29.349591861172474	31.474872879588318	20.710625834877167	18.46490942436204
8	26.997545374851782	34.58248413827171	19.685409430814474	18.73456105606203
9	26.954989335480544	14.930087149846777	18.874713879661247	39.24020963501144
10-11	25.971058930172152	24.860949162242065	27.77485781902651	21.393134088559272
12-13	26.31221969325919	22.35558800791341	27.441384679285875	23.89080761954152
14-15	23.46923692532373	24.08820650387008	24.453462632602303	27.989093938203887
16-17	23.87888995362352	27.109832695943105	25.410397178696652	23.600880171736726
18-19	23.909207505187176	26.50871522056245	26.570959828968384	23.011117445281993
20-21	24.283204692097602	25.765946428804554	26.464235975799433	23.486612903298415
22-23	24.257992837500453	25.507678844156363	26.208247888629227	24.026080429713957
24-25	24.037875736707747	25.681456543021213	26.19375316246375	24.086914557807287
26-27	23.792243908229192	25.906155644348416	26.296584802794857	24.005015644627534
28-29	23.923181680053723	26.129873832774962	26.09721966903144	23.849724818139865
30-31	24.19572313028778	25.8490014413919	25.868377086371254	24.086898341949066
32-33	23.93523919121175	25.91936099419046	25.79400364771419	24.3513961668836
34-35	24.26759995431809	25.949816829642337	25.903639680772883	23.878943535266693
36-37	24.29875772221862	25.760471964714892	26.0040084707306	23.936761842335883
38-39	24.14582918489416	25.852392644646667	25.970787285345942	24.030990885113233
40-41	24.299624155264723	25.702641777619846	26.022183332878146	23.975550734237284
42-43	24.15396016157236	25.70102630503176	25.77243005044184	24.372583482954045
44-45	23.941450053454236	25.691663600276364	26.027876038996077	24.339010307273316
46-47	24.069684750529607	25.667893726448654	25.92184423018543	24.34057729283631
48-49	23.85029070072817	25.77411393379569	25.980085101734	24.39551026374214
50-51	23.935881425565498	25.803883153065303	26.036702468169853	24.223532953199342
52-53	24.130198796697595	25.59670671972394	25.990224480994428	24.282870002584037
54-55	23.575594224899156	25.556101286208616	26.195416492780677	24.672887996111555
56-57	23.68229991575147	25.659874260499326	26.045040396973533	24.612785426775666
58-59	23.70514747308841	25.475926121029897	26.082144174630663	24.736782231251034
60-61	23.65951997099483	25.585344584242936	26.204405730862675	24.550729713899567
62-63	23.679757215361054	25.587483743440153	26.10185518022362	24.630903860975177
64-65	23.4190881534892	25.43795125161526	26.035671995618987	25.107288599276558
66-67	23.477202804048574	25.495392274167234	25.909642419094723	25.117762502689473
68-69	23.548831579759653	25.480289823827924	25.934179208314106	25.03669938809832
70-71	23.340230551271908	25.340699041546976	25.930321081011275	25.388749326169837
72-73	23.377444475799912	25.474695134765145	25.805363586692202	25.342496802742737
74-75	23.318879489646083	25.604692153242464	25.780981115241946	25.295447241869507
76-77	23.22941710639785	25.262146762482594	25.985248012599527	25.523188118520025
78-79	23.17777456719452	25.324538404399526	26.001489216695443	25.49619781171051
80-81	23.29868910300639	25.42949170627126	25.782205477619343	25.48961371310301
82-83	23.120029815291986	25.38124293323995	25.92354382903458	25.575183422433483
84-85	23.070742253182804	25.39867583473457	25.857254383857796	25.67332752822484
86-87	23.003092655948183	25.46777247085732	25.813511464870214	25.715623408324284
88-89	22.866171479807292	25.19832129878874	25.903885070337413	26.031622151066554
90-91	22.746298091994873	25.16760155310898	25.9586041283297	26.12749622656645
92-93	22.807889124903237	24.981458160301184	26.04212645925029	26.168526255545288
94-95	22.631047774407012	24.89310055293723	26.162447540746196	26.313404131909557
96-97	22.552512621638982	24.738112247041943	26.221447481375204	26.48792764994387
98-99	22.317969199704926	24.792334799699645	26.115110655735247	26.774585344860185
100-101	22.220861707199262	24.78650293068441	26.168601123955476	26.824034238160856
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	79.0
1	74.0
2	107.5
3	147.0
4	143.5
5	152.0
6	232.0
7	461.0
8	686.5
9	692.0
10	550.5
11	458.5
12	464.0
13	486.5
14	546.5
15	612.5
16	655.5
17	732.5
18	825.5
19	894.5
20	971.5
21	1093.0
22	1286.5
23	1481.5
24	1742.5
25	2150.5
26	2762.5
27	3707.0
28	5148.0
29	7532.5
30	11130.5
31	16500.0
32	24176.5
33	30634.5
34	35828.5
35	42718.5
36	51685.5
37	60904.0
38	69272.5
39	77730.5
40	85468.0
41	93199.0
42	101806.0
43	110663.0
44	118797.0
45	127813.5
46	135829.5
47	141743.5
48	147706.5
49	150703.5
50	151204.0
51	151711.0
52	151090.0
53	150125.5
54	146936.0
55	138822.5
56	127227.0
57	113647.0
58	102424.5
59	89759.0
60	73718.0
61	60663.0
62	50539.0
63	39326.0
64	28162.0
65	20712.5
66	15793.5
67	12410.5
68	9582.0
69	7217.5
70	5557.5
71	4093.5
72	2876.5
73	2155.0
74	1706.5
75	1278.0
76	872.5
77	533.5
78	305.5
79	181.5
80	98.0
81	59.5
82	43.5
83	31.5
84	19.5
85	10.5
86	8.5
87	6.5
88	4.5
89	2.5
90	3.0
91	3.5
92	2.5
93	2.0
94	0.5
95	0.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16422189523231304
2	2.7010180136893597E-4
3	0.010443936319598857
4	0.006092296186432666
5	0.024789343103415677
6	0.0024309162123204234
7	0.0
8	0.0
9	0.0
10-11	0.04653253811361502
12-13	3.901470464217964E-4
14-15	0.0050569059478517454
16-17	0.0
18-19	0.001845695642687729
20-21	1.950735232108982E-4
22-23	0.0
24-25	6.902601590539475E-4
26-27	0.0030761594044795486
28-29	0.005687143484379263
30-31	0.017256503976348685
32-33	0.012049541472180864
34-35	0.010278874107651175
36-37	0.003991504398007609
38-39	1.5005655631607554E-5
40-41	0.003481312106532952
42-43	0.00957360829296562
44-45	0.007652884372119852
46-47	0.003856453497323141
48-49	0.009003393378964532
50-51	0.00282106325874222
52-53	0.002400904901057208
54-55	0.004576724967640304
56-57	0.008043031418541648
58-59	0.015605881856871855
60-61	0.007727912650277889
62-63	0.021893251566515417
64-65	0.04024516840397146
66-67	0.058927209665322854
68-69	0.04921855047167278
70-71	0.06698524673949612
72-73	0.032922408455746975
74-75	0.026965163169998772
76-77	0.22532492496421902
78-79	0.08433178464963445
80-81	0.06546967552070375
82-83	0.027025185792525205
84-85	0.050328968988411736
86-87	0.06761548427602364
88-89	0.2889338991866034
90-91	0.2952362745518786
92-93	0.05315003224715396
94-95	0.009738670504913302
96-97	0.014915621697817908
98-99	9.303506491596682E-4
100-101	1.650622119476831E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3332077.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.00921223232323
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.90323306795676	45.19105180623245
2	11.85634549757486	13.755545245371403
3	4.1049755674460435	7.143791967014371
4	1.8658402091986441	4.3294368274802615
5	1.045180297690929	3.031504285489794
6	0.6781010067276598	2.3601663128530115
7	0.45614980509893177	1.8522623597601768
8	0.3327165718394326	1.544050098323573
9	0.2707784429857656	1.4136879750389382
>10	1.4062529020798402	14.334818787854092
>50	0.06214772674288936	2.4282579777381232
>100	0.016305927606073505	1.5420581491129435
>500	0.0014018521188731486	0.5570443081377868
>1k	5.711249333464106E-4	0.5163238995931269
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.0022622526430215E-5
2	0.0	0.0	0.0	0.0	6.0022622526430215E-5
3	0.0	0.0	0.0	0.0	6.0022622526430215E-5
4	0.0	0.0	0.0	0.0	6.0022622526430215E-5
5	0.0	0.0	0.0	0.0	6.0022622526430215E-5
6	0.0	0.0	0.0	0.0	9.003393378964532E-5
7	0.0	0.0	0.0	3.0011311263215107E-5	9.003393378964532E-5
8	0.0	0.0	0.0	3.0011311263215107E-5	9.003393378964532E-5
9	0.0	0.0	0.0	3.0011311263215107E-5	9.003393378964532E-5
10-11	0.0	0.0	0.0	3.0011311263215107E-5	9.003393378964532E-5
12-13	0.0	0.0	0.0	3.0011311263215107E-5	1.3505090068446798E-4
14-15	0.0	0.0	0.0	4.501696689482266E-5	1.650622119476831E-4
16-17	0.0	0.0	0.0	1.2004524505286043E-4	1.8006786757929064E-4
18-19	0.0	0.0	0.0	1.2004524505286043E-4	2.851074570005435E-4
20-21	0.0	0.0	0.0	1.2004524505286043E-4	4.0515270205340395E-4
22-23	0.0	0.0	0.0	1.8006786757929064E-4	4.6517532457983416E-4
24-25	0.0	0.0	0.0	5.101922914746568E-4	5.101922914746568E-4
26-27	0.0	0.0	0.0	0.0011254241723705665	5.402036027378719E-4
28-29	0.0	0.0	0.0	0.006587482822275716	5.552092583694795E-4
30-31	0.0	0.0	0.0	0.028780847501423287	5.70214914001087E-4
32-33	0.0	0.0	0.0	0.0790798051785718	6.002262252643021E-4
34-35	0.0	0.0	0.0	0.14869104165359925	6.002262252643021E-4
36-37	0.0	0.0	0.0	0.2582923503868608	6.002262252643021E-4
38-39	0.0	0.0	0.0	0.4283064286929744	6.152318808959098E-4
40-41	0.0	0.0	0.0	0.6127259364054312	6.902601590539475E-4
42-43	0.0	0.0	0.0	0.8048583511125342	7.202714703171626E-4
44-45	0.0	0.0	0.0	1.0470046160397855	7.652884372119852E-4
46-47	0.0	0.0	0.0	1.3246842735026831	7.802940928435928E-4
48-49	0.0	0.0	0.0	1.6032942816147404	8.553223710016306E-4
50-51	0.0	0.0	0.0	1.8903224625361297	8.703280266332381E-4
52-53	0.0	0.0	0.0	2.1832328604651092	9.453563047912758E-4
54-55	0.0	0.0	0.0	2.5235161132230735	0.0010203845829493134
56-57	0.0	0.0	0.0	2.91496865168482	0.0010654015498441363
58-59	0.0	0.0	0.0	3.3197312066918023	0.0010804072054757439
60-61	0.0	0.0	0.0	3.73780377824402	0.0010804072054757439
62-63	0.0	0.0	0.0	4.163919381214781	0.001140429828002174
64-65	0.0	0.0	0.0	4.631510616351303	0.001170441139265389
66-67	0.0	0.0	0.0	5.16970346123454	0.001170441139265389
68-69	0.0	0.0	0.0	5.743114579885159	0.001170441139265389
70-71	0.0	1.5005655631607554E-5	0.0	6.32961063024654	0.0012004524505286043
72-73	1.5005655631607554E-5	3.0011311263215107E-5	0.0	6.93756476816112	0.0014255372850027175
74-75	3.0011311263215107E-5	3.0011311263215107E-5	0.0	7.574960002424914	0.0014405429406343252
76-77	3.0011311263215107E-5	3.0011311263215107E-5	0.0	8.28247666545521	0.0015005655631607552
78-79	3.0011311263215107E-5	3.0011311263215107E-5	0.0	9.05889929914585	0.0015305768744239704
80-81	3.0011311263215107E-5	6.0022622526430215E-5	0.0	9.83919339198944	0.001545582530055578
82-83	3.0011311263215107E-5	6.0022622526430215E-5	0.0	10.646182546201665	0.0015905994969504006
84-85	3.0011311263215107E-5	6.0022622526430215E-5	0.0	11.483948300114314	0.0015905994969504006
86-87	6.0022622526430215E-5	6.0022622526430215E-5	0.0	12.38413758145445	0.0015905994969504006
88-89	6.0022622526430215E-5	6.0022622526430215E-5	0.0	13.347950842672603	0.0015905994969504006
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2445	0.0	44.52184	1
GTATCAA	4370	0.0	39.05076	1
TCAACGC	5230	0.0	32.033024	4
ATCAACG	5395	0.0	31.053793	3
CAACGCA	5425	0.0	30.9048	5
TATCAAC	5675	0.0	29.688885	2
AACGCAG	5855	0.0	28.712341	6
GTACATG	8375	0.0	27.811712	1
GTATACG	150	1.0542699E-6	25.352173	1
TACATGG	9070	0.0	24.95988	2
ACATGGG	9425	0.0	23.365122	3
ACGCAGA	7190	0.0	23.182348	7
CGCAGAG	7560	0.0	21.796505	8
CATGGGG	7840	0.0	21.247898	4
TAAGGCG	1150	0.0	19.411137	5
GAGTACA	6605	0.0	18.999735	1
GCAGAGT	8600	0.0	18.112051	9
AGAGTAC	7050	0.0	17.816763	10-11
ATGGGGG	4715	0.0	17.628172	5
GTATTAA	2125	0.0	17.224564	1
>>END_MODULE