FastQCFastQC Report
Mon 15 Aug 2016
SRR2921649_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921649_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3519894
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23550.046.5574461
GTATCAA42950.041.1100351
TCAACGC49050.035.8049554
CAACGCA50450.034.526655
ATCAACG52550.033.329433
AACGCAG54350.032.2059146
TATCAAC55500.030.7890382
GTACATG84600.029.062131
TACATGG90000.026.4757212
ACGCAGA65950.026.1133357
ACATGGG91650.025.273283
CGCAGAG70900.024.0915018
CATGGGG76850.023.0997964
GAGTACA65050.021.0142171
GCAGAGT80950.019.5190939
ATGGGGG47100.019.0454165
GTATTAC6300.018.8350831
AGAGTAC65850.018.77099410-11
AGTACAT68550.018.5572622
CGTATAC2854.3157343E-818.3198593
Produced by FastQC (version 0.11.5)