FastQCFastQC Report
Mon 15 Aug 2016
SRR2921649_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921649_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3519894
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24250.044.098671
GTATCAA41450.038.5273781
TCAACGC48350.032.0996864
CAACGCA50100.030.6195145
ATCAACG51550.030.2916493
TATCAAC53850.028.7334372
AACGCAG53850.028.4831856
GTACATG83100.027.5105171
TACATGG86500.026.0086542
ACATGGG88350.024.0135673
ACGCAGA67250.022.3131247
CGCAGAG70600.021.0525748
CATGGGG74400.020.7328954
GAGTACA64100.017.3505231
ATGGGGG43450.016.8331135
GCAGAGT82000.016.6784829
AGAGTAC68850.016.3119210-11
CCGTATA2355.1509363E-516.1659059
GTATTAA20650.015.8812171
GTATTAT10850.015.3317961
Produced by FastQC (version 0.11.5)