##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921649_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3519894
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.289169787499283	33.0	31.0	34.0	28.0	34.0
2	31.416566521605482	34.0	31.0	34.0	28.0	34.0
3	31.490954273054815	34.0	31.0	34.0	28.0	34.0
4	34.96550322254022	37.0	35.0	37.0	32.0	37.0
5	34.69950515555298	37.0	35.0	37.0	32.0	37.0
6	34.670275298062954	37.0	35.0	37.0	32.0	37.0
7	34.553241660118175	37.0	35.0	37.0	32.0	37.0
8	34.49450835735394	37.0	35.0	37.0	31.0	37.0
9	36.075315904399396	39.0	37.0	39.0	31.0	39.0
10-11	35.96895815612629	39.0	37.0	39.0	31.0	39.0
12-13	35.89012268551269	39.0	37.0	39.0	30.5	39.0
14-15	37.124711852118274	40.0	38.0	41.0	31.0	41.0
16-17	37.087509311359945	40.0	38.0	41.0	31.0	41.0
18-19	37.04558716824995	40.0	38.0	41.0	31.0	41.0
20-21	36.98574914471857	40.0	38.0	41.0	30.5	41.0
22-23	36.892504575421874	40.0	37.0	41.0	30.0	41.0
24-25	36.8084126965187	40.0	37.0	41.0	30.0	41.0
26-27	36.67722849040341	40.0	37.0	41.0	30.0	41.0
28-29	36.55531217701442	40.0	37.0	41.0	30.0	41.0
30-31	36.400262479495126	40.0	37.0	41.0	29.5	41.0
32-33	36.34680405148564	40.0	36.5	41.0	29.5	41.0
34-35	36.47216620727784	40.0	37.0	41.0	30.0	41.0
36-37	36.46457592188855	40.0	37.0	41.0	30.0	41.0
38-39	36.35739329082069	40.0	37.0	41.0	29.0	41.0
40-41	36.275641113056246	40.0	36.5	41.0	29.0	41.0
42-43	36.151907415393765	40.0	36.0	41.0	28.5	41.0
44-45	36.022043419489336	40.0	36.0	41.0	28.0	41.0
46-47	35.873053279445344	40.0	36.0	41.0	27.5	41.0
48-49	35.728837004750716	40.0	35.0	41.0	27.0	41.0
50-51	35.57093778960389	39.5	35.0	41.0	27.0	41.0
52-53	35.39707360505743	39.0	35.0	41.0	26.0	41.0
54-55	35.18401733120372	39.0	35.0	41.0	26.0	41.0
56-57	34.96037778410373	39.0	35.0	41.0	26.0	41.0
58-59	34.71911014933973	38.5	35.0	40.5	25.5	41.0
60-61	34.44500388363967	38.0	34.0	40.0	25.0	41.0
62-63	34.14374680032978	37.5	34.0	40.0	24.0	41.0
64-65	33.810157209279595	37.0	34.0	40.0	23.0	41.0
66-67	33.464664987070634	36.0	34.0	39.0	22.5	41.0
68-69	33.10807967512658	36.0	33.5	39.0	22.0	41.0
70-71	32.71299732321485	35.0	33.0	38.5	21.0	40.0
72-73	32.35197082639421	35.0	33.0	37.5	20.0	40.0
74-75	31.949902326604153	35.0	33.0	37.0	19.0	39.0
76-77	29.23600625473381	33.0	29.0	34.5	11.5	36.5
78-79	30.805154217712236	34.5	31.5	36.0	13.0	37.5
80-81	30.98232120058161	35.0	32.0	35.5	14.5	37.0
82-83	30.797315061192183	35.0	32.0	35.0	11.5	37.0
84-85	30.589200839570736	35.0	32.0	35.0	8.5	36.5
86-87	30.372872023987085	35.0	32.0	35.0	6.0	36.0
88-89	30.09200703202994	35.0	31.0	35.0	2.0	36.0
90-91	29.884130885759628	34.0	31.0	35.0	2.0	35.0
92-93	29.715545127211218	34.0	31.0	35.0	2.0	35.0
94-95	29.596433443734384	34.0	31.0	35.0	2.0	35.0
96-97	29.378903171515958	34.0	31.0	35.0	2.0	35.0
98-99	29.164089458375734	34.0	31.0	35.0	2.0	35.0
100-101	28.039392805578803	33.0	28.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	73242.0
3	24119.0
4	12935.0
5	9083.0
6	8156.0
7	8033.0
8	8305.0
9	9096.0
10	9663.0
11	10524.0
12	11448.0
13	11946.0
14	12761.0
15	13622.0
16	14176.0
17	15203.0
18	14436.0
19	13546.0
20	13290.0
21	13039.0
22	13980.0
23	15886.0
24	18454.0
25	22681.0
26	26975.0
27	31780.0
28	38745.0
29	46411.0
30	56333.0
31	69934.0
32	88512.0
33	116106.0
34	164895.0
35	247090.0
36	433095.0
37	828588.0
38	882023.0
39	111782.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.41247055147669	19.512671935411447	12.66880523865718	26.406052274454687
2	15.542828505574185	21.111274310794638	36.910076942810946	26.435820240820227
3	17.92559271559965	24.56425178312617	29.197183366713826	28.312972134560354
4	12.965500547066936	16.527018515040716	35.479343428761545	35.0281375091308
5	13.998803642496553	37.391485969795525	34.534493317021955	14.075217070685971
6	33.80633940067526	35.68225492836582	16.691639477838123	13.8197661931208
7	28.880443558811713	31.72547241479431	21.162938429395886	18.23114559699809
8	26.692593583784056	34.73124474771115	19.982505154984782	18.59365651352001
9	26.646541060611483	15.006162117381944	19.19293592363861	39.15436089836796
10-11	25.568971373430145	25.176047080425267	28.17281960983277	21.082161936311817
12-13	25.941884464975416	22.69819894684059	27.778038202952654	23.581878385231335
14-15	23.150330431442416	24.349546334200493	24.796755647791517	27.703367586565562
16-17	23.558667328997313	27.42727869465369	25.673688706774794	23.340365269574207
18-19	23.534946778800613	26.837343082266855	26.865923943090564	22.761786195841967
20-21	24.028302633225607	26.1022769355074	26.692935521037693	23.1764849102293
22-23	23.8897108833391	25.898535580901015	26.43652053158419	23.775233004175693
24-25	23.705425352327154	26.011852101860235	26.49620072491066	23.786521820901953
26-27	23.486390412177062	26.253566255790815	26.48177537460957	23.778267957422557
28-29	23.60763011833789	26.44121624242001	26.361664222572912	23.58948941666919
30-31	23.914206319517483	26.16290410872492	26.174468897041702	23.74842067471589
32-33	23.64884792574353	26.252941487345566	26.05650610687153	24.041704480039368
34-35	24.00114390403708	26.22311924958077	26.20611411877622	23.569622727605932
36-37	24.002606426600213	26.044307263432483	26.27314379113471	23.679942518832597
38-39	23.83716668740593	26.17404103646303	26.266827921522633	23.721964354608403
40-41	23.981051015742093	26.025263323575583	26.29069286272257	23.70299279795975
42-43	23.88212662699046	25.97340274325852	26.11306455171344	24.03140607803758
44-45	23.70309400434021	26.04760535576589	26.30116778789685	23.948132851997048
46-47	23.870290827530745	25.998500184600314	26.11239978561078	24.01880920225816
48-49	23.61739934384235	26.152161294199512	26.200419879525434	24.03001948243271
50-51	23.715536354947844	26.098188645023747	26.239006465147412	23.947268534881
52-53	23.914258951637166	25.957325792062175	26.160261671654123	23.968153584646533
54-55	23.38328394999294	25.917630917939178	26.43492720917408	24.2641579228938
56-57	23.4906659760625	25.98265126937583	26.266687331755794	24.259995422805876
58-59	23.512583024577307	25.860492711113043	26.351547275083327	24.27537698922632
60-61	23.47518336013765	25.957877279935516	26.422696951354034	24.144242408572797
62-63	23.476337224914644	25.92115676153373	26.365058458967898	24.237447554583728
64-65	23.24329002335814	25.779205101303166	26.311552438863046	24.665952436475646
66-67	23.214620491059403	25.955109759572913	26.17429489006276	24.655974859304923
68-69	23.380219197371577	25.85177139087308	26.19113975555643	24.576869656198912
70-71	23.215503602695662	25.667028191857582	26.221554766802036	24.89591343864472
72-73	23.179803567802555	25.870128076384585	26.127679081075065	24.82238927473779
74-75	23.177247141719423	25.979282128082517	26.009561111044015	24.83390961915405
76-77	23.14938007159947	25.700365685501925	26.195867049081016	24.95438719381759
78-79	23.10571003121738	25.757964782563075	26.18255130651522	24.953773879704325
80-81	23.187502256533858	25.892926437991303	25.973692582051832	24.945878723423007
82-83	23.13749756673539	25.84432505857596	26.049770740295003	24.968406634393645
84-85	23.05546384781306	25.778124996002838	26.06594913391966	25.10046202226445
86-87	22.96167458378619	25.961177353238096	25.985854089415643	25.09129397356007
88-89	22.872799356725178	25.732616962555227	26.015645346315846	25.37893833440375
90-91	22.731835255192713	25.727815813811144	26.116167569287313	25.424181361708833
92-93	22.861468724271003	25.54125671414434	26.158219656146553	25.439054905438102
94-95	22.73220558098898	25.454196751703883	26.19999076583216	25.613606901474984
96-97	22.64324376775261	25.355641830307157	26.21769793598383	25.7834164659564
98-99	22.478776132446253	25.33519818129027	26.16940797043517	26.01661771582831
100-101	22.358309331609036	25.36519648647712	26.219584952467212	26.056909229446635
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	61.0
1	55.5
2	86.5
3	128.0
4	120.5
5	129.5
6	224.5
7	433.0
8	604.5
9	628.5
10	528.5
11	418.5
12	413.5
13	463.5
14	500.5
15	566.5
16	642.5
17	667.0
18	717.0
19	790.5
20	894.5
21	1063.0
22	1261.5
23	1477.0
24	1812.5
25	2311.0
26	3091.5
27	4276.5
28	6125.5
29	9198.0
30	14092.5
31	20947.0
32	30502.0
33	38531.0
34	45152.5
35	53734.0
36	64137.0
37	74548.0
38	83379.5
39	92264.5
40	99913.0
41	107086.5
42	115107.5
43	123171.0
44	131332.0
45	139975.5
46	145422.0
47	149258.0
48	153917.0
49	155379.5
50	154430.5
51	153522.0
52	152091.5
53	150043.0
54	145767.5
55	136933.5
56	125128.5
57	111849.5
58	100354.0
59	87447.5
60	71391.0
61	58600.0
62	48692.0
63	37633.0
64	27112.5
65	20126.0
66	15181.0
67	11694.0
68	9143.0
69	6971.5
70	5228.5
71	3734.5
72	2677.0
73	2062.5
74	1563.5
75	1100.5
76	737.0
77	454.5
78	262.5
79	154.0
80	101.0
81	68.5
82	40.0
83	25.0
84	17.0
85	12.5
86	10.0
87	6.0
88	4.5
89	3.5
90	2.0
91	1.5
92	1.5
93	1.5
94	1.0
95	1.5
96	1.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1622492040953506
2	1.9886962505120893E-4
3	0.010596910020585848
4	0.006108138483715702
5	0.024972342917144664
6	0.0018750564647685415
7	0.0
8	0.0
9	0.0
10-11	0.04713210113713651
12-13	2.5568951792298295E-4
14-15	0.005042765492369941
16-17	1.4204973217943495E-5
18-19	0.0015909570004096715
20-21	2.698944911409264E-4
22-23	0.0
24-25	3.4091935723064385E-4
26-27	0.002727354857845151
28-29	0.00524163511742115
30-31	0.01677607337039127
32-33	0.011818537717328988
34-35	0.010454860288406413
36-37	0.003764317902755026
38-39	0.0
40-41	0.003622268170575591
42-43	0.00910538783270178
44-45	0.0070456667160999735
46-47	0.003622268170575591
48-49	0.008892313234432628
50-51	0.002784174750716925
52-53	0.0017898266254608806
54-55	0.004289901911818936
56-57	0.007684890510907432
58-59	0.015043066637802163
60-61	0.007088281635753804
62-63	0.021563149344838228
64-65	0.04035632891217747
66-67	0.05941940297065764
68-69	0.049447511771661304
70-71	0.06787136203533402
72-73	0.03333907214251338
74-75	0.02703206403374647
76-77	0.22718013667457032
78-79	0.08361047236081541
80-81	0.06622358514205257
82-83	0.027245138632015626
84-85	0.050385040004045575
86-87	0.06849638085692354
88-89	0.2940997655043021
90-91	0.30044938853272285
92-93	0.052686245665352426
94-95	0.010128145904393713
96-97	0.014702147280571518
98-99	0.0010227580716919318
100-101	2.2727957148709592E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3519894.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.596766625597716
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.96559601583466	45.68531834565495
2	12.141307729679992	14.228827511312483
3	4.002302615984445	7.0356597706157915
4	1.8257084318082948	4.279224436202265
5	1.008671679358905	2.9552449498621742
6	0.6416761669113811	2.256008916102857
7	0.44064976810191436	1.8074456137564143
8	0.3258059200981641	1.5272938772184208
9	0.25235796039766856	1.3308624460380614
>10	1.3102135847585672	13.550266580599061
>50	0.06430243219077927	2.542480465369057
>100	0.019707022910925652	1.8570754161471779
>500	0.001068993815348616	0.4262908197898686
>1k	6.316781489667943E-4	0.5180008513314609
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.840994643588699E-5
2	0.0	0.0	0.0	0.0	2.840994643588699E-5
3	0.0	0.0	0.0	0.0	2.840994643588699E-5
4	0.0	0.0	0.0	0.0	2.840994643588699E-5
5	0.0	0.0	0.0	0.0	2.840994643588699E-5
6	0.0	0.0	0.0	0.0	2.840994643588699E-5
7	0.0	0.0	0.0	0.0	2.840994643588699E-5
8	0.0	0.0	0.0	0.0	2.840994643588699E-5
9	0.0	0.0	0.0	2.840994643588699E-5	2.840994643588699E-5
10-11	0.0	0.0	0.0	2.840994643588699E-5	2.840994643588699E-5
12-13	0.0	0.0	0.0	2.840994643588699E-5	7.102486608971748E-5
14-15	0.0	0.0	0.0	5.681989287177398E-5	1.4204973217943497E-4
16-17	0.0	0.0	0.0	5.681989287177398E-5	1.8466465183326544E-4
18-19	0.0	0.0	0.0	5.681989287177398E-5	1.9886962505120893E-4
20-21	0.0	0.0	0.0	1.4204973217943497E-4	2.2727957148709592E-4
22-23	0.0	0.0	0.0	2.556895179229829E-4	2.9830443757681343E-4
24-25	0.0	0.0	0.0	5.539939554997963E-4	3.409193572306439E-4
26-27	0.0	0.0	0.0	0.0011079879109995926	3.693293036665309E-4
28-29	0.0	0.0	0.0	0.0041052372599856704	3.693293036665309E-4
30-31	0.0	0.0	0.0	0.017259042459801345	3.693293036665309E-4
32-33	0.0	0.0	0.0	0.052927730210057464	3.9773925010241787E-4
34-35	0.0	0.0	0.0	0.10356845973202602	3.9773925010241787E-4
36-37	0.0	0.0	0.0	0.18950854770058417	3.9773925010241787E-4
38-39	0.0	0.0	0.0	0.32621720995007236	4.1194422332036136E-4
40-41	0.0	0.0	0.0	0.4767331061674016	4.2614919653830485E-4
42-43	0.0	0.0	0.0	0.6379595521910603	4.2614919653830485E-4
44-45	0.0	0.0	0.0	0.8367155374565257	4.4035416975624834E-4
46-47	0.0	0.0	0.0	1.0693361788735682	4.829690894100788E-4
48-49	0.0	0.0	0.0	1.3053802188361354	5.966088751536267E-4
50-51	0.0	0.0	0.0	1.5535553059268263	5.966088751536267E-4
52-53	0.0	0.0	0.0	1.8094578984480783	6.534287680254008E-4
54-55	0.0	0.0	0.0	2.1077907459713274	6.818387144612878E-4
56-57	0.0	0.0	0.0	2.450087985604112	7.102486608971748E-4
58-59	0.0	0.0	0.0	2.809274938393031	7.528635805510053E-4
60-61	0.0	0.0	0.0	3.179271875800805	7.670685537689488E-4
62-63	0.0	0.0	0.0	3.553430870361437	7.670685537689488E-4
64-65	0.0	0.0	0.0	3.9628892233686583	7.812735269868922E-4
66-67	0.0	0.0	0.0	4.4418383053580595	7.954785002048357E-4
68-69	0.0	0.0	0.0	4.951058185274897	7.954785002048357E-4
70-71	0.0	0.0	0.0	5.47316197590041	8.522983930766097E-4
72-73	0.0	0.0	0.0	6.014882834539904	9.517332056022141E-4
74-75	0.0	0.0	0.0	6.578919706104786	9.80143152038101E-4
76-77	0.0	0.0	0.0	7.224890863190767	9.943481252560446E-4
78-79	0.0	0.0	0.0	7.929542764640072	0.001036963044909875
80-81	0.0	0.0	0.0	8.644237582154464	0.001093782937781649
82-83	0.0	0.0	0.0	9.379870530192102	0.0011648078038713665
84-85	0.0	0.0	0.0	10.135262595975902	0.0011648078038713665
86-87	0.0	0.0	0.0	10.948852437033615	0.0011790127770893101
88-89	0.0	0.0	0.0	11.825796458643357	0.0011932177503072535
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2425	0.0	44.09867	1
GTATCAA	4145	0.0	38.527378	1
TCAACGC	4835	0.0	32.099686	4
CAACGCA	5010	0.0	30.619514	5
ATCAACG	5155	0.0	30.291649	3
TATCAAC	5385	0.0	28.733437	2
AACGCAG	5385	0.0	28.483185	6
GTACATG	8310	0.0	27.510517	1
TACATGG	8650	0.0	26.008654	2
ACATGGG	8835	0.0	24.013567	3
ACGCAGA	6725	0.0	22.313124	7
CGCAGAG	7060	0.0	21.052574	8
CATGGGG	7440	0.0	20.732895	4
GAGTACA	6410	0.0	17.350523	1
ATGGGGG	4345	0.0	16.833113	5
GCAGAGT	8200	0.0	16.678482	9
AGAGTAC	6885	0.0	16.31192	10-11
CCGTATA	235	5.1509363E-5	16.165905	9
GTATTAA	2065	0.0	15.881217	1
GTATTAT	1085	0.0	15.331796	1
>>END_MODULE