FastQCFastQC Report
Mon 15 Aug 2016
SRR2921648_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921648_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2567008
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18950.044.5784381
GTATCAA32000.038.5600361
TCAACGC37250.032.365384
CAACGCA38850.031.2749635
ATCAACG40050.030.1014613
TATCAAC40450.029.8032112
AACGCAG41250.029.6709946
GTACATG62450.026.5980421
ACGCAGA47500.024.9737787
TACATGG66950.023.8197172
CGCAGAG49900.023.5842938
ACATGGG67100.022.0692943
TAAGGCG8250.020.135485
GAGTACA48000.019.477761
CATGGGG53400.019.377094
GCAGAGT60600.018.6370039
GTATTAA15600.018.557491
GTATAGG7100.018.04761
AGAGTAC49600.017.65203910-11
TAAGGTG11600.017.5937485
Produced by FastQC (version 0.11.5)