##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921648_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2567008
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.95124985975891	33.0	31.0	34.0	28.0	34.0
2	31.020206014161232	33.0	31.0	34.0	28.0	34.0
3	31.08274458046099	34.0	31.0	34.0	27.0	34.0
4	34.53253632244231	37.0	35.0	37.0	32.0	37.0
5	34.53188459093232	37.0	35.0	37.0	32.0	37.0
6	34.58376600306661	37.0	35.0	37.0	32.0	37.0
7	34.50956366322193	37.0	35.0	37.0	32.0	37.0
8	34.48481539597851	37.0	35.0	37.0	32.0	37.0
9	36.11598950996647	39.0	37.0	39.0	32.0	39.0
10-11	36.02711639387178	39.0	37.0	39.0	32.0	39.0
12-13	35.936099147334176	39.0	37.0	39.0	31.0	39.0
14-15	37.16947239743702	40.0	38.0	41.0	31.0	41.0
16-17	37.11401444794874	40.0	38.0	41.0	31.0	41.0
18-19	37.07882425765716	40.0	38.0	41.0	31.0	41.0
20-21	37.0058614932248	40.0	38.0	41.0	31.0	41.0
22-23	36.90776168208279	40.0	38.0	41.0	30.5	41.0
24-25	36.80791158422568	40.0	37.0	41.0	30.0	41.0
26-27	36.672930703760954	40.0	37.0	41.0	30.0	41.0
28-29	36.54648836310599	40.0	37.0	41.0	30.0	41.0
30-31	36.40997982865655	40.0	37.0	41.0	30.0	41.0
32-33	36.24644722571959	40.0	36.5	41.0	29.0	41.0
34-35	36.07916317362471	40.0	36.0	41.0	28.5	41.0
36-37	36.053523985901094	40.0	36.5	41.0	28.5	41.0
38-39	36.238409268689466	40.0	37.0	41.0	29.5	41.0
40-41	36.30184381973098	40.0	37.0	41.0	29.0	41.0
42-43	36.149214766763485	40.0	37.0	41.0	28.0	41.0
44-45	36.03529926669492	40.0	36.0	41.0	28.0	41.0
46-47	35.88090473422755	40.0	36.0	41.0	27.5	41.0
48-49	35.72410311927349	40.0	36.0	41.0	27.0	41.0
50-51	34.026229953315294	38.0	33.0	39.5	24.0	40.0
52-53	34.157958993505275	38.5	34.0	39.5	23.5	40.0
54-55	35.06103214325783	39.0	35.0	40.5	26.0	41.0
56-57	35.06817664767698	39.0	35.0	41.0	26.0	41.0
58-59	34.87313518306137	39.0	35.0	41.0	26.0	41.0
60-61	34.61824778107431	38.5	35.0	41.0	25.0	41.0
62-63	34.32341406805121	37.5	34.5	40.0	24.0	41.0
64-65	34.02444694367918	37.0	34.0	40.0	23.5	41.0
66-67	33.676778958226855	36.5	34.0	39.5	23.0	41.0
68-69	33.31531826157145	36.0	34.0	39.0	22.0	41.0
70-71	32.9047102696992	35.5	34.0	39.0	21.5	41.0
72-73	32.535316796831175	35.0	33.0	37.5	20.0	40.0
74-75	32.12806037223102	35.0	33.0	37.0	18.5	39.0
76-77	31.756278710467598	35.0	33.0	37.0	17.5	39.0
78-79	31.412708296974532	35.0	33.0	36.0	14.0	39.0
80-81	31.099352631546143	35.0	32.5	36.0	9.5	37.0
82-83	30.792578363604633	35.0	32.0	35.0	6.0	37.0
84-85	30.533092222540795	35.0	32.0	35.0	2.0	36.5
86-87	30.30475869183111	35.0	32.0	35.0	2.0	36.0
88-89	30.0872628756903	35.0	32.0	35.0	2.0	36.0
90-91	29.867464378763135	35.0	32.0	35.0	2.0	35.5
92-93	29.680656234807216	35.0	31.0	35.0	2.0	35.0
94-95	29.453969757788055	35.0	31.0	35.0	2.0	35.0
96-97	29.219015873733156	35.0	31.0	35.0	2.0	35.0
98-99	28.962645811777758	35.0	31.0	35.0	2.0	35.0
100-101	27.898402926675725	34.0	28.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	67477.0
3	12167.0
4	8285.0
5	6574.0
6	5936.0
7	6090.0
8	6380.0
9	6620.0
10	7483.0
11	7764.0
12	7963.0
13	8041.0
14	8612.0
15	9306.0
16	9191.0
17	9790.0
18	9434.0
19	8979.0
20	9273.0
21	9769.0
22	10427.0
23	12063.0
24	14267.0
25	16879.0
26	20117.0
27	23647.0
28	27970.0
29	33070.0
30	39392.0
31	48283.0
32	61182.0
33	80506.0
34	114109.0
35	174074.0
36	310581.0
37	592883.0
38	646404.0
39	116013.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.25007969708094	17.845053285974952	12.467975023831182	25.436891993112926
2	16.45539670951313	19.4255582792864	38.77600499236606	25.34304001883441
3	19.49705586929907	22.99423178788071	29.069816842992296	28.438895499827915
4	13.51999005301292	15.237365436012848	35.834683569268954	35.40796094170528
5	14.488750966115507	36.7406858280063	34.239484395407544	14.531078810470646
6	34.28350919514973	34.80680913265918	16.64212237239694	14.267559299794138
7	29.357621593570332	31.151345663173373	20.922053650427177	18.568979092829117
8	26.76831273347245	34.40926113226248	19.931631630539638	18.89079450372543
9	26.78741521311695	15.111748634624877	19.20002399803341	38.90081215422476
10-11	25.841240206545248	24.778027363135134	27.847531160129062	21.533201270190556
12-13	26.209743955298304	22.266804778212716	27.515381209102035	24.008070057386945
14-15	23.39212166015419	23.928928696827363	24.605617320298244	28.07333232272021
16-17	23.798458704214287	26.900221008941667	25.545606083512702	23.755714203331344
18-19	23.79272567325333	26.286830092338455	26.69436833228597	23.226075902122254
20-21	24.27775326341382	25.628023911732704	26.491254216551773	23.6029686083017
22-23	24.175160221657578	25.408944433825674	26.260472396944834	24.155422947571918
24-25	23.96801793745039	25.622259864681986	26.258674619123383	24.151047578744244
26-27	23.76997377741585	25.776040428756787	26.304778121091456	24.149207672735916
28-29	23.982760672843366	25.939503005720017	26.10798851984239	23.96974780159423
30-31	24.09022577482439	25.70650694891815	25.956809429404238	24.246457846853218
32-33	23.825294961921042	25.797655576732176	25.946694914858035	24.430354546488743
34-35	24.221696202058233	25.800704673095264	25.976249618211174	24.001349506635332
36-37	24.37278729345475	25.54834607976963	25.964611498594465	24.114255128181156
38-39	24.040979152017112	25.766357670589013	26.014905051394376	24.177758125999496
40-41	24.27194432043332	25.562919608846833	26.105406599365633	24.05972947135421
42-43	24.127775145591965	25.55218011332015	25.933185757472394	24.38685898361549
44-45	24.00388924091132	25.599032059705777	26.097145965146655	24.299932734236247
46-47	24.18359208556851	25.635376297598107	25.902348670132653	24.278682946700734
48-49	23.966867330012054	25.72823546719102	25.87097738410804	24.43391981868889
50-51	23.97495228491044	25.794905200949643	26.045074103489895	24.18506841065002
52-53	24.262642497772383	25.585536123693792	25.975903294766823	24.175918083767005
54-55	23.81591480119668	25.47592262869821	26.205175750041263	24.502986820063846
56-57	23.863014243855844	25.627370772995402	26.109671017384272	24.399943965764486
58-59	23.920004604837615	25.449805806583157	26.15850202290907	24.471687565670155
60-61	23.835372609348397	25.512765426866462	26.27419140198616	24.37767056179898
62-63	23.909772969155295	25.4934772408236	26.332358797115994	24.264390992905103
64-65	23.77765607693765	25.434255109890717	26.170319740450736	24.61776907272089
66-67	23.736481903001565	25.476248866236713	26.13142699169214	24.655842239069585
68-69	23.90412450135158	25.428299195295377	26.203857463026665	24.463718840326376
70-71	23.764684125606518	25.222235812538685	26.173115792032263	24.839964269822534
72-73	23.78621002075415	25.34382473337415	26.088188161589727	24.78177708428198
74-75	23.75619058767296	25.50238258977998	26.134254178677164	24.607172643869895
76-77	23.80432621843031	25.16755263996242	26.224360861420042	24.803760280187227
78-79	23.719062010317398	25.253598395826373	26.238256153842705	24.789083440013535
80-81	23.87395535687977	25.328957574615135	26.113409503225327	24.68367756527977
82-83	23.7689659290388	25.306065741008865	26.23001938058616	24.694948949366168
84-85	23.739856402819807	25.33479309686127	26.204368324796473	24.72098217552245
86-87	23.774792059047208	25.36179998676859	26.172858009617556	24.69054994456664
88-89	23.644465224905122	25.17268120501	26.205704704796908	24.977148865287976
90-91	23.647176833111835	25.225195688701284	26.228940656218224	24.898686821968656
92-93	23.782266493584757	25.01305529904636	26.42310565464226	24.781572552726626
94-95	23.663898528983285	24.859766794174167	26.46056130752011	25.015773369322442
96-97	23.743741051675062	24.73221381363752	26.436435471355214	25.087609663332206
98-99	23.6097092607988	24.752311589366492	26.367652970816795	25.27032617901791
100-101	23.806879759273023	24.58856324381862	26.44893558761805	25.15562140929031
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	39.0
1	32.0
2	52.5
3	76.0
4	66.0
5	77.5
6	135.0
7	252.0
8	404.0
9	459.5
10	413.5
11	398.0
12	419.5
13	452.5
14	538.5
15	623.5
16	693.0
17	775.0
18	846.5
19	929.5
20	1007.5
21	1110.5
22	1199.0
23	1319.0
24	1504.0
25	1739.5
26	2151.0
27	2811.5
28	3869.5
29	5567.0
30	8496.5
31	12962.5
32	18964.5
33	24182.5
34	28997.0
35	35038.0
36	42463.0
37	50100.0
38	56782.5
39	62940.5
40	68924.0
41	74805.0
42	80487.5
43	86569.5
44	92495.5
45	99389.0
46	104155.5
47	107477.0
48	110933.0
49	112149.0
50	111604.0
51	110791.5
52	110692.0
53	110584.5
54	108706.0
55	103432.5
56	95463.5
57	85965.0
58	78675.5
59	70105.0
60	58119.5
61	48200.5
62	40169.5
63	31175.5
64	22512.0
65	16655.5
66	12890.5
67	10272.5
68	7986.0
69	6110.5
70	4720.5
71	3456.5
72	2510.0
73	1932.0
74	1519.5
75	1202.0
76	885.0
77	616.0
78	437.0
79	301.5
80	208.5
81	143.5
82	119.5
83	98.5
84	82.5
85	74.5
86	53.5
87	41.0
88	39.0
89	33.0
90	31.5
91	27.0
92	22.5
93	20.0
94	15.0
95	10.0
96	12.0
97	12.0
98	8.5
99	6.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.040592004387987886
2	0.058083184781660205
3	0.05434342238123138
4	0.05481089268128499
5	0.05146068886423416
6	0.00857028883431606
7	0.010946596192921876
8	0.012621698101447287
9	0.005103217442251835
10-11	0.011939970580535784
12-13	0.01883515750632643
14-15	0.035410875229060446
16-17	0.02321769156932896
18-19	0.00993374387613907
20-21	0.01891306922300203
22-23	0.03106729702439572
24-25	0.029489584761714805
26-27	0.020179134618980542
28-29	0.012407440880589387
30-31	0.012212661588900385
32-33	0.009193582567720864
34-35	0.005609643600643239
36-37	6.232937334048044E-4
38-39	0.0036228948254154254
40-41	0.0013244991834852093
42-43	0.18348209277103927
44-45	0.1573816676847131
46-47	0.16345878158540994
48-49	0.169107381044391
50-51	0.16125777558932422
52-53	0.16721802191500768
54-55	0.16552344207731334
56-57	0.16708167641082536
58-59	0.17473650257420312
60-61	0.17837887532878743
62-63	0.16558187586482007
64-65	0.16585456687318464
66-67	0.16336139193956545
68-69	0.1589399020182251
70-71	0.15225897231329236
72-73	0.16154994452685772
74-75	0.16149151073935103
76-77	0.15611560228873458
78-79	0.1730614006656777
80-81	0.18346261484187038
82-83	0.18114474127077126
84-85	0.17500919358256772
86-87	0.19176021266782184
88-89	0.1858973559879829
90-91	0.19100057343023472
92-93	0.18815679577157532
94-95	0.19230559468455102
96-97	0.1977399369226742
98-99	0.19158491130530172
100-101	0.19873331131028807
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2567008.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.837263768754525
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.04579346331828	49.498128439704594
2	11.076491317930905	13.698798305184257
3	3.5380689848990086	6.5635351515374865
4	1.6422888064308705	4.0621858443095515
5	0.9383631461258483	2.901290468893121
6	0.5822981689583473	2.1604635279564066
7	0.41833186389279564	1.8107948467289465
8	0.30533258254776424	1.510474515536089
9	0.23201370706290397	1.2912383521453807
>10	1.1612306291829178	12.366158237576668
>50	0.04621662131571148	1.9376302086666721
>100	0.01205370574202623	1.3298648740214702
>500	9.481266203534367E-4	0.4146282193842426
>1k	5.688759721919975E-4	0.4548090083551673
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1686757501340082E-4
2	0.0	0.0	0.0	0.0	1.558234333512011E-4
3	0.0	0.0	0.0	0.0	1.558234333512011E-4
4	0.0	0.0	0.0	0.0	1.9477929168900136E-4
5	0.0	3.895585833780027E-5	0.0	0.0	1.9477929168900136E-4
6	0.0	3.895585833780027E-5	0.0	0.0	1.9477929168900136E-4
7	0.0	3.895585833780027E-5	0.0	0.0	1.9477929168900136E-4
8	0.0	3.895585833780027E-5	0.0	0.0	1.9477929168900136E-4
9	0.0	3.895585833780027E-5	0.0	0.0	2.3373515002680163E-4
10-11	0.0	3.895585833780027E-5	0.0	3.895585833780027E-5	2.3373515002680163E-4
12-13	0.0	3.895585833780027E-5	0.0	3.895585833780027E-5	2.726910083646019E-4
14-15	0.0	3.895585833780027E-5	0.0	3.895585833780027E-5	3.311247958713023E-4
16-17	0.0	3.895585833780027E-5	0.0	3.895585833780027E-5	4.6747030005360327E-4
18-19	0.0	3.895585833780027E-5	0.0	3.895585833780027E-5	4.6747030005360327E-4
20-21	0.0	3.895585833780027E-5	0.0	3.895585833780027E-5	5.64859945898104E-4
22-23	0.0	3.895585833780027E-5	0.0	9.738964584450068E-5	6.232937334048044E-4
24-25	0.0	3.895585833780027E-5	0.0	4.0903651254690286E-4	6.622495917426046E-4
26-27	0.0	3.895585833780027E-5	0.0	6.817275209115047E-4	6.622495917426046E-4
28-29	0.0	3.895585833780027E-5	0.0	0.0029216893753350203	7.012054500804049E-4
30-31	0.0	3.895585833780027E-5	0.0	0.011881536793029083	7.012054500804049E-4
32-33	0.0	3.895585833780027E-5	0.0	0.03544983108739825	7.791171667560054E-4
34-35	0.0	3.895585833780027E-5	0.0	0.06579644473254466	7.791171667560054E-4
36-37	0.0	3.895585833780027E-5	0.0	0.11795833904685922	7.791171667560054E-4
38-39	0.0	3.895585833780027E-5	0.0	0.2028041985065882	7.791171667560054E-4
40-41	0.0	3.895585833780027E-5	0.0	0.3017520786846009	7.985950959249056E-4
42-43	0.0	3.895585833780027E-5	0.0	0.40792627058427555	8.180730250938057E-4
44-45	0.0	3.895585833780027E-5	0.0	0.5443107306249143	8.180730250938057E-4
46-47	0.0	3.895585833780027E-5	0.0	0.7002315536219599	8.180730250938057E-4
48-49	0.0	3.895585833780027E-5	0.0	0.8633786883406673	8.57028883431606E-4
50-51	0.0	3.895585833780027E-5	0.0	1.0401408955484361	8.959847417694063E-4
52-53	0.0	3.895585833780027E-5	0.0	1.2228828270110572	9.738964584450067E-4
54-55	0.0	3.895585833780027E-5	0.0	1.432017352497538	0.0010907640334584076
56-57	0.0	3.895585833780027E-5	0.0	1.6795428763759208	0.0010907640334584076
58-59	0.0	3.895585833780027E-5	0.0	1.9378007392263679	0.0011102419626273078
60-61	0.0	3.895585833780027E-5	0.0	2.21208893778282	0.0011297198917962078
62-63	0.0	3.895585833780027E-5	0.0	2.4962524464279037	0.0011297198917962078
64-65	0.0	3.895585833780027E-5	0.0	2.8095744150388313	0.0011297198917962078
66-67	0.0	3.895585833780027E-5	0.0	3.176694424014261	0.0011297198917962078
68-69	0.0	3.895585833780027E-5	0.0	3.5731481943180543	0.0011297198917962078
70-71	0.0	5.843378750670041E-5	0.0	3.9846389259402386	0.0011297198917962078
72-73	0.0	7.791171667560054E-5	0.0	4.414614212343709	0.0012271095376407085
74-75	0.0	7.791171667560054E-5	0.0	4.869073255712487	0.0012465874668096087
76-77	0.0	7.791171667560054E-5	0.0	5.393321719293434	0.0012465874668096087
78-79	0.0	7.791171667560054E-5	0.0	5.971329267380545	0.0012465874668096087
80-81	0.0	7.791171667560054E-5	0.0	6.564841247086102	0.0012465874668096087
82-83	0.0	7.791171667560054E-5	0.0	7.17496790037273	0.0012855433251474089
84-85	0.0	7.791171667560054E-5	0.0	7.816921489921341	0.0012855433251474089
86-87	0.0	7.791171667560054E-5	0.0	8.50778415961306	0.001324499183485209
88-89	0.0	7.791171667560054E-5	0.0	9.26341094379137	0.0013634550418230094
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1895	0.0	44.578438	1
GTATCAA	3200	0.0	38.560036	1
TCAACGC	3725	0.0	32.36538	4
CAACGCA	3885	0.0	31.274963	5
ATCAACG	4005	0.0	30.101461	3
TATCAAC	4045	0.0	29.803211	2
AACGCAG	4125	0.0	29.670994	6
GTACATG	6245	0.0	26.598042	1
ACGCAGA	4750	0.0	24.973778	7
TACATGG	6695	0.0	23.819717	2
CGCAGAG	4990	0.0	23.584293	8
ACATGGG	6710	0.0	22.069294	3
TAAGGCG	825	0.0	20.13548	5
GAGTACA	4800	0.0	19.47776	1
CATGGGG	5340	0.0	19.37709	4
GCAGAGT	6060	0.0	18.637003	9
GTATTAA	1560	0.0	18.55749	1
GTATAGG	710	0.0	18.0476	1
AGAGTAC	4960	0.0	17.652039	10-11
TAAGGTG	1160	0.0	17.593748	5
>>END_MODULE