FastQCFastQC Report
Mon 15 Aug 2016
SRR2921648_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921648_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2567008
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18850.049.9234541
GTATCAA32400.039.753471
TCAACGC40600.031.561814
CAACGCA41900.030.602245
TATCAAC42400.030.1111622
ATCAACG42600.030.081213
AACGCAG45100.028.3222946
GTACATG62150.025.1597081
TACATGG64750.023.7490542
ACGCAGA56400.022.3101527
ACATGGG66750.022.0420273
CGCAGAG58100.021.4939068
CATGGGG56250.019.827574
GCAGAGT64750.017.8931089
AGAGTAC54400.017.54417410-11
GTATTAA16450.016.757641
CCCTATA4559.094947E-1216.6897452
ATGGGGG29400.016.6377035
GAGTACA49100.016.455771
TAAGGTG12650.015.7674795
Produced by FastQC (version 0.11.5)