##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921648_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2567008
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.54892310425211	31.0	31.0	34.0	27.0	34.0
2	30.708793661726023	33.0	31.0	34.0	27.0	34.0
3	30.76877789239457	33.0	31.0	34.0	27.0	34.0
4	34.247509162417884	37.0	35.0	37.0	31.0	37.0
5	33.93014513394582	37.0	35.0	37.0	30.0	37.0
6	33.88476857103679	37.0	35.0	37.0	30.0	37.0
7	33.73272346638577	37.0	35.0	37.0	29.0	37.0
8	33.665516040464226	37.0	35.0	37.0	28.0	37.0
9	35.16666562784378	39.0	35.0	39.0	28.0	39.0
10-11	35.025001090764036	39.0	35.0	39.0	28.0	39.0
12-13	34.92256977773346	39.0	35.0	39.0	27.0	39.0
14-15	36.05998228287563	40.0	36.0	41.0	27.0	41.0
16-17	36.00497524744761	40.0	36.0	41.0	27.0	41.0
18-19	35.94923369931064	40.0	36.5	41.0	27.0	41.0
20-21	35.87746220502624	40.0	36.0	41.0	27.0	41.0
22-23	35.77223016056047	40.0	36.0	41.0	27.0	41.0
24-25	35.671409477492865	40.0	36.0	41.0	26.5	41.0
26-27	35.527988225981375	40.0	36.0	41.0	25.0	41.0
28-29	35.38806930091375	40.0	36.0	41.0	25.0	41.0
30-31	35.21732246257121	39.5	35.5	41.0	24.0	41.0
32-33	35.16046814034082	39.5	35.0	41.0	24.0	41.0
34-35	35.28987424269811	40.0	36.0	41.0	24.5	41.0
36-37	35.277784292062975	40.0	35.5	41.0	24.0	41.0
38-39	35.16101566492976	40.0	35.0	41.0	24.0	41.0
40-41	35.06219594952567	40.0	35.0	41.0	23.0	41.0
42-43	34.92725441447787	40.0	35.0	41.0	22.5	41.0
44-45	34.7836263073586	39.0	35.0	41.0	21.5	41.0
46-47	34.613400698400625	39.0	35.0	41.0	20.5	41.0
48-49	34.45377556283424	39.0	35.0	41.0	18.5	41.0
50-51	34.28802656633715	39.0	34.5	41.0	18.0	41.0
52-53	34.1031292072327	39.0	34.0	41.0	15.5	41.0
54-55	33.88422903239881	38.5	34.0	40.0	13.5	41.0
56-57	33.64384178000225	38.0	34.0	40.0	12.5	41.0
58-59	33.39729950198831	38.0	33.5	40.0	8.5	41.0
60-61	33.11104815411561	37.0	33.0	40.0	7.5	41.0
62-63	32.8077072607487	37.0	33.0	40.0	6.5	41.0
64-65	32.47460039080517	36.0	33.0	39.5	2.0	41.0
66-67	32.12521912670315	36.0	32.0	39.0	2.0	41.0
68-69	31.769323079632006	35.0	32.0	39.0	2.0	40.0
70-71	31.38439186788666	35.0	32.0	38.0	2.0	40.0
72-73	31.032971264600654	35.0	31.0	37.0	2.0	39.5
74-75	30.639767776337276	35.0	31.0	37.0	2.0	39.0
76-77	28.037990921726774	32.0	27.5	34.5	2.0	36.0
78-79	29.56077522937209	34.0	30.0	35.0	2.0	37.0
80-81	29.75045773133547	35.0	31.0	35.0	2.0	37.0
82-83	29.58861133272666	35.0	31.0	35.0	2.0	37.0
84-85	29.38725979817749	34.0	30.5	35.0	2.0	36.0
86-87	29.172822016916193	34.0	30.0	35.0	2.0	36.0
88-89	28.906624171019335	34.0	30.0	35.0	2.0	35.5
90-91	28.701365948216754	34.0	30.0	35.0	2.0	35.0
92-93	28.52987758511076	34.0	30.0	35.0	2.0	35.0
94-95	28.407707143881126	34.0	29.5	35.0	2.0	35.0
96-97	28.17009335381892	34.0	29.0	35.0	2.0	35.0
98-99	27.93668562778145	34.0	29.0	35.0	2.0	35.0
100-101	26.80917628616662	33.0	26.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	93157.0
3	26567.0
4	13665.0
5	9259.0
6	7785.0
7	7629.0
8	7695.0
9	8098.0
10	8870.0
11	9430.0
12	9767.0
13	10163.0
14	10858.0
15	11583.0
16	11909.0
17	12499.0
18	11820.0
19	10831.0
20	10606.0
21	10588.0
22	11092.0
23	12530.0
24	14708.0
25	17510.0
26	21082.0
27	25455.0
28	30477.0
29	36650.0
30	44263.0
31	54683.0
32	69460.0
33	91201.0
34	129323.0
35	195408.0
36	342010.0
37	601870.0
38	518532.0
39	47975.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.303291566618455	20.939719880613037	13.640491090443582	27.116497462324922
2	14.6526456522857	22.058360641432582	35.973843437719616	27.315150268562107
3	16.992097179178007	25.293195236880088	28.985294948389146	28.72941263555276
4	13.001019140836226	17.521197038569962	34.224938719092684	35.25284510150113
5	14.100070445803487	37.2971955712656	34.378759966834366	14.223974016096555
6	34.2288943679228	35.89635510061135	16.145537303507616	13.72921322795824
7	28.91253163215697	31.673722871140253	20.9781971852055	18.43554831149728
8	26.637509505229435	35.36849125518892	19.462035178698315	18.531964060883332
9	26.463709083656212	15.097705264420885	18.93419805017986	39.50438760174304
10-11	25.851108687976566	25.314301681289912	27.76024296124015	21.07434666949337
12-13	26.14891343070894	22.651486569057322	27.508826932902064	23.690773067331673
14-15	23.35004082726638	24.26429110152588	24.48301694799043	27.902651123217314
16-17	23.765819974070983	27.38353367032748	25.330072987696184	23.52057336790536
18-19	23.81085574755003	26.66332161005262	26.534668083640963	22.99115455875639
20-21	24.308902518266432	25.9037578437743	26.34164185174624	23.44569778621303
22-23	24.185101877360726	25.6755919732233	26.156229353395084	23.983076796020892
24-25	23.973252050398898	25.946372040052196	26.080848055931426	23.99952785361748
26-27	23.805825704147505	26.086955674854007	26.175251071163597	23.931967549834894
28-29	24.003027824467992	26.204239071129525	26.043127798775117	23.749605305627362
30-31	24.141625717487287	26.038788340666002	25.846744092175005	23.972841849671706
32-33	23.966070209669024	26.042306265759464	25.742389485068834	24.249234039502678
34-35	24.21470417788773	26.067623439805594	25.87481433503942	23.842858047267253
36-37	24.34454404660501	25.870527666366243	25.89565491445146	23.889273372577293
38-39	24.181074655995356	25.967688057008015	25.912117514265155	23.93911977273148
40-41	24.263253432482358	25.836058561449647	25.98649232219043	23.914195683877566
42-43	24.192459101145612	25.812829606790892	25.74083305234029	24.253878239723203
44-45	24.105323277314625	25.840095589057437	25.88185931157646	24.17272182205148
46-47	24.268421314057147	25.87382238675288	25.744580763353426	24.11317553583655
48-49	23.99610715151175	26.001827986900206	25.763765029659986	24.23829983192806
50-51	24.078714292448293	25.981630189226113	25.813452573094153	24.12620294523144
52-53	24.317622215993502	25.802588001417238	25.759618911308173	24.120170871281086
54-55	23.858324235196495	25.776578327897177	25.932816305938932	24.432281130967397
56-57	23.926488162778718	25.876817318078267	25.863025813294584	24.333668705848435
58-59	23.975313230771146	25.72022232975589	25.86206157627215	24.44240286320081
60-61	23.844392763579393	25.776237264648127	26.031630949016126	24.347739022756354
62-63	23.902227097078367	25.81063586675408	25.993163728602557	24.293973307565
64-65	23.74421785356514	25.689049468614435	25.855535535051803	24.711197142768622
66-67	23.72687885238774	25.792607430300613	25.80589900237202	24.67461471493963
68-69	23.83269525103858	25.71209867170013	25.834791315325162	24.620414761936125
70-71	23.720566709611028	25.581688161585138	25.828634466843514	24.869110661960327
72-73	23.689914258002243	25.755157359927395	25.720007933950257	24.83492044812011
74-75	23.658680758772952	25.873944124621932	25.61567889698771	24.851696219617402
76-77	23.68157988314192	25.586759259006264	25.82919547166158	24.90246538619023
78-79	23.607535165763707	25.67470608701754	25.823916325315356	24.8938424219034
80-81	23.754006680431885	25.770005726195844	25.60997239030281	24.86601520306946
82-83	23.597268718913675	25.752067680782154	25.722414573430697	24.92824902687347
84-85	23.51915907793223	25.728080038818984	25.724299475203203	25.028461408045583
86-87	23.540872146769292	25.82983008814164	25.605962828991412	25.02333493609765
88-89	23.37689299985547	25.701459714306917	25.636930732903924	25.284716552933684
90-91	23.213727305914063	25.8176277461868	25.675395099907593	25.293249847991543
92-93	23.348190249853804	25.66225259122408	25.7233028069264	25.26625435199571
94-95	23.163171029079265	25.530481696138903	25.825713745295932	25.480633529485903
96-97	23.15542382604952	25.386609109140796	25.839835664952084	25.618131399857603
98-99	23.02565981447925	25.50161366004276	25.699296510836326	25.773430014641658
100-101	22.891019474254914	25.510843475342565	25.71614112869689	25.88199592170563
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	34.0
1	27.5
2	36.0
3	55.5
4	59.0
5	54.0
6	92.0
7	240.5
8	368.0
9	392.0
10	340.0
11	286.0
12	286.5
13	307.5
14	347.5
15	370.5
16	399.5
17	462.0
18	536.0
19	591.0
20	685.5
21	778.0
22	897.0
23	1096.0
24	1286.5
25	1601.5
26	2067.0
27	2847.0
28	4102.0
29	6082.5
30	9328.0
31	13855.0
32	20056.5
33	25525.5
34	30059.5
35	35756.5
36	43049.5
37	50495.5
38	57323.0
39	63726.5
40	69554.0
41	75646.5
42	81495.0
43	87163.0
44	93217.5
45	100287.0
46	105428.5
47	109116.0
48	112554.5
49	113526.0
50	112668.5
51	111762.0
52	110971.5
53	110155.0
54	108493.5
55	102796.5
56	94430.0
57	85150.5
58	76862.5
59	68007.5
60	56788.5
61	46991.0
62	38852.5
63	30102.0
64	21803.5
65	16129.0
66	12503.0
67	9896.0
68	7620.5
69	5729.0
70	4282.0
71	3124.5
72	2212.0
73	1669.0
74	1283.5
75	979.5
76	711.0
77	464.5
78	279.5
79	163.5
80	94.5
81	52.0
82	28.0
83	19.0
84	16.5
85	11.0
86	9.5
87	7.5
88	4.5
89	3.0
90	1.5
91	2.0
92	1.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12637280444782406
2	1.558234333512011E-4
3	0.007908039242573455
4	0.005453820167292038
5	0.019322105735548933
6	0.001986748775227814
7	0.0
8	0.0
9	7.791171667560054E-5
10-11	0.03903377005447588
12-13	2.5321307919570177E-4
14-15	0.0037397624004288257
16-17	0.0
18-19	0.0014024109001608098
20-21	1.558234333512011E-4
22-23	0.0
24-25	2.9216893753350204E-4
26-27	0.0022010059960857154
28-29	0.005784944963163341
30-31	0.016049813635173712
32-33	0.01153093406798888
34-35	0.00858976676348496
36-37	0.0028632555878283198
38-39	1.9477929168900136E-5
40-41	0.0037981961879355267
42-43	0.00851185504680936
44-45	0.00728474550916865
46-47	0.00430462234632693
48-49	0.009738964584450067
50-51	0.003077512808686221
52-53	0.0018114474127077127
54-55	0.003525505179570925
56-57	0.008200208180106958
58-59	0.0144136675849861
60-61	0.00726526757999975
62-63	0.02144520001495905
64-65	0.04002714444208978
66-67	0.057362501402410906
68-69	0.04857795534723694
70-71	0.06517315099913985
72-73	0.031982759695334026
74-75	0.025477131352921376
76-77	0.19855800994776798
78-79	0.0715034779790324
80-81	0.06252415263216944
82-83	0.02596407958214388
84-85	0.048811690497263735
86-87	0.06421873246986375
88-89	0.2694966279809023
90-91	0.27726832171929344
92-93	0.0423644959423578
94-95	0.008317075755120357
96-97	0.011706235430508981
98-99	6.817275209115047E-4
100-101	1.3634550418230095E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2567008.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.311854312991336
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.9513853365307	51.25182314861255
2	10.605415280166918	13.428970142934002
3	3.426526635911342	6.508192655172095
4	1.554761903728016	3.937394365608689
5	0.8638899378128886	2.7347236942634376
6	0.5616730955643556	2.1336339118738405
7	0.3938508757750341	1.7454800487679107
8	0.2833706794946821	1.4352578541392542
9	0.21171621440909788	1.206373151013016
>10	1.0950425773103587	11.939751229586431
>50	0.040609558287890646	1.7170025915021714
>100	0.010338854451338352	1.1703125069448574
>500	9.87165605151604E-4	0.44831518354360594
>1k	4.3188495225008537E-4	0.3427695160381268
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.895585833780027E-5
2	0.0	0.0	0.0	0.0	7.791171667560054E-5
3	0.0	0.0	0.0	0.0	7.791171667560054E-5
4	0.0	0.0	0.0	0.0	7.791171667560054E-5
5	0.0	0.0	0.0	0.0	7.791171667560054E-5
6	0.0	0.0	0.0	0.0	1.1686757501340082E-4
7	0.0	0.0	0.0	0.0	1.1686757501340082E-4
8	0.0	0.0	0.0	0.0	1.1686757501340082E-4
9	0.0	0.0	0.0	3.895585833780027E-5	1.1686757501340082E-4
10-11	0.0	0.0	0.0	3.895585833780027E-5	1.1686757501340082E-4
12-13	0.0	0.0	0.0	3.895585833780027E-5	1.7530136252010123E-4
14-15	0.0	0.0	0.0	5.843378750670041E-5	2.3373515002680163E-4
16-17	0.0	0.0	0.0	7.791171667560054E-5	2.726910083646019E-4
18-19	0.0	0.0	0.0	7.791171667560054E-5	3.116468667024022E-4
20-21	0.0	0.0	0.0	7.791171667560054E-5	3.116468667024022E-4
22-23	0.0	0.0	0.0	9.738964584450068E-5	3.311247958713023E-4
24-25	0.0	0.0	0.0	3.895585833780027E-4	3.5060272504020245E-4
26-27	0.0	0.0	0.0	6.427716625737045E-4	3.5060272504020245E-4
28-29	0.0	0.0	0.0	0.0026295204378015183	3.5060272504020245E-4
30-31	0.0	0.0	0.0	0.010810250688739574	3.5060272504020245E-4
32-33	0.0	0.0	0.0	0.03322934716214363	3.895585833780027E-4
34-35	0.0	0.0	0.0	0.06197877061544023	4.28514441715803E-4
36-37	0.0	0.0	0.0	0.11118001969608196	4.28514441715803E-4
38-39	0.0	0.0	0.0	0.19027989005098545	4.28514441715803E-4
40-41	0.0	0.0	0.0	0.2831311783991324	4.4799237088470313E-4
42-43	0.0	3.895585833780027E-5	0.0	0.38246861716052305	5.064261583914035E-4
44-45	0.0	3.895585833780027E-5	0.0	0.5113735524003042	5.453820167292038E-4
46-47	0.0	3.895585833780027E-5	0.0	0.6586851307046959	6.622495917426046E-4
48-49	0.0	3.895585833780027E-5	0.0	0.8136904910308032	7.401613084182052E-4
50-51	0.0	3.895585833780027E-5	0.0	0.9802462633540683	8.180730250938057E-4
52-53	0.0	3.895585833780027E-5	0.0	1.1534440095239282	8.765068126005062E-4
54-55	0.0	7.791171667560054E-5	0.0	1.3523526221967366	9.349406001072065E-4
56-57	0.0	7.791171667560054E-5	0.0	1.5877433962028946	9.349406001072065E-4
58-59	0.0	7.791171667560054E-5	0.0	1.833593818172752	9.349406001072065E-4
60-61	0.0	7.791171667560054E-5	0.0	2.094792848327703	9.349406001072065E-4
62-63	0.0	7.791171667560054E-5	0.0	2.3653607624128945	9.349406001072065E-4
64-65	0.0	7.791171667560054E-5	0.0	2.664853401313903	9.738964584450068E-4
66-67	0.0	1.1686757501340082E-4	0.0	3.013313554145527	0.001032330245951707
68-69	0.0	1.1686757501340082E-4	0.0	3.391010078659669	0.0010518081751206072
70-71	0.0	1.3634550418230095E-4	0.0	3.783801998279709	0.0010518081751206072
72-73	0.0	1.558234333512011E-4	0.0	4.197727471048006	0.0011686757501340082
74-75	0.0	1.558234333512011E-4	0.0	4.636604171081664	0.0011686757501340082
76-77	0.0	1.558234333512011E-4	0.0	5.14104358069784	0.0011686757501340082
78-79	0.0	1.558234333512011E-4	0.0	5.698540869370099	0.0011686757501340082
80-81	0.0	1.558234333512011E-4	0.0	6.271114075219088	0.0011686757501340082
82-83	0.0	1.558234333512011E-4	0.0	6.863827459828719	0.0011686757501340082
84-85	0.0	1.558234333512011E-4	0.0	7.484939665166607	0.0012076316084718083
86-87	0.0	1.558234333512011E-4	0.0	8.155623200239344	0.0012076316084718083
88-89	0.0	1.558234333512011E-4	0.0	8.884350964235406	0.0012076316084718083
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1885	0.0	49.923454	1
GTATCAA	3240	0.0	39.75347	1
TCAACGC	4060	0.0	31.56181	4
CAACGCA	4190	0.0	30.60224	5
TATCAAC	4240	0.0	30.111162	2
ATCAACG	4260	0.0	30.08121	3
AACGCAG	4510	0.0	28.322294	6
GTACATG	6215	0.0	25.159708	1
TACATGG	6475	0.0	23.749054	2
ACGCAGA	5640	0.0	22.310152	7
ACATGGG	6675	0.0	22.042027	3
CGCAGAG	5810	0.0	21.493906	8
CATGGGG	5625	0.0	19.82757	4
GCAGAGT	6475	0.0	17.893108	9
AGAGTAC	5440	0.0	17.544174	10-11
GTATTAA	1645	0.0	16.75764	1
CCCTATA	455	9.094947E-12	16.689745	2
ATGGGGG	2940	0.0	16.637703	5
GAGTACA	4910	0.0	16.45577	1
TAAGGTG	1265	0.0	15.767479	5
>>END_MODULE