FastQCFastQC Report
Mon 15 Aug 2016
SRR2921647_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921647_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2524877
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18550.051.43581
GTATCAA31750.038.5735241
TCAACGC39250.030.115514
CAACGCA39900.029.7427065
ATCAACG40700.029.1580813
TATCAAC41600.028.4142762
GTACATG61500.028.4044281
AACGCAG42950.027.9433276
TACATGG64000.026.7767452
ACATGGG67950.024.5205943
ACGCAGA51300.022.8429377
GTATAGA7400.022.451721
CATGGGG57600.022.0048964
GTATTAG6450.020.6068521
CGCAGAG56250.020.4110458
GAGTACA47600.019.9451071
TAAGGCG8400.018.6486765
GTATTAA12950.018.3279361
TAAGGTG11450.018.2414995
CATACCG2101.9682044E-518.0835655
Produced by FastQC (version 0.11.5)