##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921647_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2524877
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.604469445442295	31.0	31.0	34.0	27.0	34.0
2	30.673921937583494	33.0	31.0	34.0	27.0	34.0
3	30.73069222777981	33.0	31.0	34.0	27.0	34.0
4	34.19318604431028	37.0	35.0	37.0	30.0	37.0
5	34.193054156697535	37.0	35.0	37.0	30.0	37.0
6	34.236690737806235	37.0	35.0	37.0	30.0	37.0
7	34.14619801281409	37.0	35.0	37.0	30.0	37.0
8	34.122614289725796	37.0	35.0	37.0	30.0	37.0
9	35.70226272408517	39.0	37.0	39.0	30.0	39.0
10-11	35.61231596628271	39.0	37.0	39.0	30.0	39.0
12-13	35.502857564942765	39.0	37.0	39.0	30.0	39.0
14-15	36.67584539761739	40.0	37.0	41.0	30.0	41.0
16-17	36.6127526212168	40.0	37.0	41.0	30.0	41.0
18-19	36.57332832450848	40.0	37.0	41.0	30.0	41.0
20-21	36.4950108460729	40.0	37.0	41.0	29.0	41.0
22-23	36.39802572560961	40.0	37.0	41.0	29.0	41.0
24-25	36.288006504871326	40.0	37.0	41.0	28.0	41.0
26-27	36.14041792926943	40.0	36.0	41.0	27.5	41.0
28-29	36.00361958226084	40.0	36.0	41.0	27.0	41.0
30-31	35.858272303957776	40.0	36.0	41.0	27.0	41.0
32-33	35.67938477795156	40.0	36.0	41.0	26.5	41.0
34-35	35.50579691604779	39.5	35.0	41.0	25.5	41.0
36-37	35.46851173344286	39.5	35.0	41.0	25.5	41.0
38-39	35.65226009029351	40.0	35.5	41.0	26.5	41.0
40-41	35.71248381604332	40.0	36.0	41.0	26.0	41.0
42-43	35.550271557782814	40.0	35.0	41.0	25.0	41.0
44-45	35.425509440657905	40.0	35.0	41.0	25.0	41.0
46-47	35.2622430716427	40.0	35.0	41.0	24.0	41.0
48-49	35.08842668375529	40.0	35.0	41.0	24.0	41.0
50-51	33.37354671138436	37.0	32.5	39.5	20.5	40.0
52-53	33.49567365063724	37.5	33.5	39.5	21.0	40.0
54-55	34.40754876376156	39.0	34.0	40.5	22.0	41.0
56-57	34.41539667080812	39.0	34.0	41.0	22.0	41.0
58-59	34.21475224337661	38.0	34.0	41.0	22.0	41.0
60-61	33.95791795006252	38.0	34.0	40.0	20.5	41.0
62-63	33.653253207978054	37.0	34.0	40.0	20.0	41.0
64-65	33.3528595650402	37.0	34.0	40.0	18.5	41.0
66-67	33.00381860185665	36.0	33.0	39.0	17.5	41.0
68-69	32.63717480099031	36.0	33.0	39.0	14.0	41.0
70-71	32.223132651610356	35.0	33.0	39.0	9.5	40.5
72-73	31.851481280078197	35.0	32.5	37.5	7.5	40.0
74-75	31.447066530369597	35.0	32.0	37.0	4.0	39.0
76-77	31.065555470622925	35.0	32.0	36.5	2.0	39.0
78-79	30.719602578660265	35.0	32.0	36.0	2.0	38.0
80-81	30.402292666137797	35.0	31.5	35.5	2.0	37.0
82-83	30.089563174760592	35.0	31.0	35.0	2.0	37.0
84-85	29.82636243270464	35.0	31.0	35.0	2.0	36.0
86-87	29.59196962861953	35.0	31.0	35.0	2.0	36.0
88-89	29.361494639144798	35.0	31.0	35.0	2.0	36.0
90-91	29.124953611601676	35.0	31.0	35.0	2.0	35.0
92-93	28.920439688745233	34.5	30.0	35.0	2.0	35.0
94-95	28.683258233965457	34.0	30.0	35.0	2.0	35.0
96-97	28.42749825040982	34.0	30.0	35.0	2.0	35.0
98-99	28.145402132460312	34.0	29.5	35.0	2.0	35.0
100-101	27.057955892504864	33.0	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	77088.0
3	13692.0
4	9533.0
5	7379.0
6	6869.0
7	6940.0
8	7429.0
9	7894.0
10	8669.0
11	9023.0
12	9214.0
13	9206.0
14	9829.0
15	10441.0
16	10376.0
17	11127.0
18	10673.0
19	10248.0
20	10478.0
21	10992.0
22	12059.0
23	13874.0
24	16515.0
25	18912.0
26	22614.0
27	27138.0
28	31367.0
29	37203.0
30	44338.0
31	53810.0
32	66817.0
33	86882.0
34	121934.0
35	180276.0
36	306947.0
37	556197.0
38	574362.0
39	96524.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.47727187172472	17.711673835558628	12.349210563334239	25.461843729382416
2	16.576560723320654	19.32991457869378	38.52717703846118	25.56634765952438
3	19.558239600701167	22.92813595944552	29.10539417245325	28.40823026740007
4	13.702927212054439	15.067255332472	35.56031578697061	35.669501668502946
5	14.68088715289612	36.57147680742898	34.09409394334738	14.653542096327518
6	34.62383982356128	34.26968695437247	16.61516048381219	14.491312738254056
7	29.506556431577348	31.16570754956143	20.68020997059271	18.64752604826852
8	26.97905476278754	34.20776807019879	19.861492706639922	18.951684460373748
9	26.974426085100095	15.158110539738848	19.087178345646905	38.78028502951415
10-11	26.097610005594973	24.652185753118083	27.660023667232764	21.590180574054177
12-13	26.403182441744434	22.203350484936525	27.368420635640607	24.025046437678437
14-15	23.625340117692854	23.822214650294132	24.436967165456423	28.115478066556587
16-17	24.010635255042494	26.84155981627781	25.33104935097451	23.816755577705184
18-19	24.037995172770415	26.244154356478646	26.556199278589215	23.161651192161724
20-21	24.462960905284994	25.51773763523153	26.46177607890603	23.55752538057744
22-23	24.43671519480324	25.29349842355531	26.118249595686905	24.151536785954548
24-25	24.204220741596203	25.488608236854382	26.160812889436063	24.146358132113352
26-27	23.972502756597265	25.6872478731072	26.181781521419857	24.158467848875684
28-29	24.11962570030674	25.92911453976221	25.974329480061854	23.97693027986919
30-31	24.37783050752125	25.619505718400067	25.84938248963653	24.153281284442155
32-33	24.087146437210386	25.715958336320494	25.7794512045623	24.417444021906825
34-35	24.478238982052602	25.709085234399847	25.846345425142204	23.96633035840535
36-37	24.524325582086153	25.547965483268893	25.932818123759215	23.994890810885742
38-39	24.299057462059057	25.621549450466752	25.873017004656646	24.206376082817545
40-41	24.430431736367808	25.491088385017573	25.93115395720381	24.147325921410808
42-43	24.323772439630318	25.490078569340675	25.761666980536564	24.424482010492447
44-45	24.108404916263893	25.583490406111743	25.97552263524378	24.332582042380587
46-47	24.3590486318329	25.514372103358518	25.806714653037904	24.31986461177068
48-49	24.115184629774177	25.58826645671866	25.847473937386233	24.449074976120926
50-51	24.158386966217236	25.67291744371523	25.96793137107078	24.200764218996753
52-53	24.42400071268894	25.484581851788874	25.860310983961128	24.23110645156106
54-55	23.90673816296418	25.33106916487063	26.152554401448658	24.609638270716534
56-57	24.010881489107696	25.517314174526433	26.005100276402203	24.466704059963664
58-59	24.045902632115215	25.34270318278171	26.070264743355327	24.54112944174775
60-61	24.011079701382243	25.331015901224845	26.216205382850553	24.441699014542355
62-63	24.011656675664984	25.39104583506772	26.205398116980234	24.391899372287064
64-65	23.844332346274225	25.273970904076705	26.12815896115749	24.753537788491585
66-67	23.894729365530335	25.289823788406412	26.015315742859958	24.8001311032033
68-69	23.949139788210083	25.272087769595487	26.151731920316013	24.627040521878417
70-71	23.84796349714665	25.096267130550153	26.08759391388994	24.968175458413256
72-73	23.8451824612604	25.237615777366212	25.98309454535715	24.934107216016237
74-75	23.865601602397565	25.349358896145517	26.05997047484082	24.725069026616094
76-77	23.8873506444194	25.011550885693545	26.116170664658494	24.98492780522856
78-79	23.84016816383513	25.063975156388672	26.16835392026782	24.927502759508375
80-81	23.972689047698516	25.143685716479897	26.05605504101068	24.827570194810903
82-83	23.89749640532175	25.1622913389983	26.109865590981773	24.830346664698176
84-85	23.832616881179003	25.178524373130823	26.097437757557675	24.891420988132502
86-87	23.87331970168075	25.21364866734157	26.013071065259467	24.899960565718214
88-89	23.777481912894142	24.977262746207796	26.083931474094413	25.16132386680365
90-91	23.800520398481314	24.92691261127829	26.212964872209447	25.05960211803095
92-93	23.86911856518531	24.777348198087175	26.263129004487766	25.09040423223975
94-95	23.836734272660863	24.682144924648526	26.319025381371514	25.162095421319098
96-97	23.85602345757494	24.48398277041597	26.334815441686217	25.325178330322874
98-99	23.726835704148748	24.443270012580268	26.320704971359497	25.509189311911484
100-101	23.992689913978673	24.361945318047887	26.329666779001286	25.315697988972154
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	50.0
1	40.5
2	65.0
3	95.0
4	93.5
5	107.0
6	179.0
7	346.0
8	514.5
9	553.5
10	514.0
11	468.0
12	487.5
13	555.5
14	588.0
15	659.0
16	759.5
17	848.0
18	924.0
19	1023.5
20	1137.5
21	1206.0
22	1319.5
23	1519.0
24	1714.0
25	1947.0
26	2383.0
27	3127.0
28	4070.0
29	5707.0
30	8351.5
31	12063.0
32	17453.0
33	22040.5
34	25881.0
35	30974.0
36	37372.0
37	44065.5
38	51270.0
39	58555.0
40	65068.5
41	71166.0
42	77172.5
43	83912.5
44	89890.0
45	96381.0
46	101529.0
47	105233.0
48	109076.5
49	111288.0
50	111954.0
51	112178.5
52	112068.5
53	111576.5
54	110228.0
55	105458.5
56	97177.0
57	87361.5
58	78929.0
59	69583.0
60	58134.0
61	48532.5
62	40559.0
63	31615.0
64	22983.5
65	17171.0
66	13324.0
67	10577.5
68	8278.5
69	6279.0
70	4863.5
71	3596.5
72	2616.5
73	2006.0
74	1547.5
75	1229.5
76	935.5
77	638.0
78	407.0
79	298.5
80	233.0
81	152.5
82	111.5
83	98.0
84	70.5
85	51.5
86	49.5
87	45.0
88	40.0
89	34.5
90	28.0
91	24.0
92	21.5
93	16.5
94	16.0
95	17.5
96	11.5
97	7.5
98	9.0
99	7.5
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.049190515023108054
2	0.06875582454115586
3	0.06550814158471878
4	0.06562695925385673
5	0.062141640959143755
6	0.010337137215000968
7	0.01318876127431158
8	0.015406691098219835
9	0.006178518795172993
10-11	0.011465905071811418
12-13	0.02083269798885253
14-15	0.037011703936468986
16-17	0.024991316408680502
18-19	0.009525216475891697
20-21	0.01808008865382353
22-23	0.03338776502776175
24-25	0.03146687937669835
26-27	0.018951418227501775
28-29	0.010653984332702147
30-31	0.010436151939282587
32-33	0.007287483707127119
34-35	0.004356647868391212
36-37	5.742854008333871E-4
38-39	0.003881377191839444
40-41	0.001029753132528832
42-43	0.20814875338481834
44-45	0.18014738935797664
46-47	0.18682098177455772
48-49	0.19359358891542044
50-51	0.1844446283917989
52-53	0.19183112682320763
54-55	0.18798935552108081
56-57	0.1908013736906788
58-59	0.19888097519205886
60-61	0.20333663778473168
62-63	0.1891577292676039
64-65	0.18870226153590847
66-67	0.1871378288922589
68-69	0.18204847206418373
70-71	0.17491941191590718
72-73	0.1855337903588967
74-75	0.18430600777780462
76-77	0.1785235478797581
78-79	0.19618777469159887
80-81	0.20707939436257686
82-83	0.20515850871151348
84-85	0.20012856071800725
86-87	0.21765416691585374
88-89	0.21123801278240484
90-91	0.2163075666656237
92-93	0.21228756885978997
94-95	0.21587190187878458
96-97	0.22201081478424495
98-99	0.2160699313273478
100-101	0.22250588840565305
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2524877.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.24379910264275
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.07898600583367	48.43097532181975
2	11.581240671032734	14.185583540321685
3	3.7436759522347254	6.878308137721752
4	1.7679845242145058	4.331123560702984
5	0.9825576443258193	3.0087781487928194
6	0.6022818406018685	2.213161682939444
7	0.43179139774670366	1.8511181932493903
8	0.3218924538997013	1.5771133423432
9	0.22764441393821064	1.2547627878663559
>10	1.2088052516710097	12.621016610870758
>50	0.04087916501579739	1.6568994037345413
>100	0.01083744195516162	1.21666330350516
>500	9.70389225123847E-4	0.41315960378966343
>1k	4.528483050490149E-4	0.36133636234250666
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	3.9605889712647385E-5	0.0
8	0.0	0.0	0.0	3.9605889712647385E-5	0.0
9	0.0	0.0	0.0	3.9605889712647385E-5	3.9605889712647385E-5
10-11	0.0	0.0	0.0	3.9605889712647385E-5	3.9605889712647385E-5
12-13	0.0	0.0	0.0	3.9605889712647385E-5	5.940883456897108E-5
14-15	0.0	0.0	0.0	3.9605889712647385E-5	9.901472428161847E-5
16-17	0.0	0.0	0.0	5.940883456897108E-5	1.1881766913794217E-4
18-19	0.0	0.0	0.0	7.921177942529477E-5	1.1881766913794217E-4
20-21	0.0	0.0	0.0	7.921177942529477E-5	1.1881766913794217E-4
22-23	0.0	0.0	0.0	7.921177942529477E-5	1.3862061399426586E-4
24-25	0.0	0.0	0.0	2.1783239341956065E-4	1.5842355885058954E-4
26-27	0.0	0.0	0.0	6.53497180258682E-4	1.5842355885058954E-4
28-29	0.0	0.0	0.0	0.003425909460143999	1.5842355885058954E-4
30-31	0.0	0.0	0.0	0.015980976499053223	1.7822650370691323E-4
32-33	0.0	0.0	0.0	0.048794456125981586	1.9802944856323694E-4
34-35	0.0	0.0	0.0	0.09358871739098579	1.9802944856323694E-4
36-37	0.0	0.0	0.0	0.1617900594761646	1.9802944856323694E-4
38-39	0.0	0.0	0.0	0.27551837178603156	1.9802944856323694E-4
40-41	0.0	0.0	0.0	0.39827682695038213	1.9802944856323694E-4
42-43	0.0	0.0	0.0	0.5328378372491016	1.9802944856323694E-4
44-45	0.0	0.0	0.0	0.7033807983517613	2.1783239341956065E-4
46-47	1.9802944856323693E-5	0.0	0.0	0.8992319229808026	2.3763533827588434E-4
48-49	3.9605889712647385E-5	0.0	0.0	1.1019150635852757	2.3763533827588434E-4
50-51	3.9605889712647385E-5	0.0	0.0	1.3089350491132836	2.3763533827588434E-4
52-53	3.9605889712647385E-5	0.0	0.0	1.5209255738002287	2.772412279885317E-4
54-55	3.9605889712647385E-5	0.0	0.0	1.7779281921455976	2.772412279885317E-4
56-57	3.9605889712647385E-5	0.0	0.0	2.070259264114648	3.168471177011791E-4
58-59	3.9605889712647385E-5	0.0	0.0	2.3755810679094465	3.564530074138265E-4
60-61	3.9605889712647385E-5	0.0	0.0	2.697022468817293	3.960588971264739E-4
62-63	3.9605889712647385E-5	0.0	0.0	3.027414800800197	4.3566478683912125E-4
64-65	3.9605889712647385E-5	0.0	0.0	3.3917097743771283	4.752706765517687E-4
66-67	3.9605889712647385E-5	0.0	0.0	3.8235327899141227	4.752706765517687E-4
68-69	3.9605889712647385E-5	0.0	0.0	4.277752936083619	4.752706765517687E-4
70-71	3.9605889712647385E-5	0.0	0.0	4.744191499229467	4.950736214080924E-4
72-73	3.9605889712647385E-5	0.0	0.0	5.233839113746927	5.742854008333871E-4
74-75	3.9605889712647385E-5	0.0	0.0	5.756953705071574	5.940883456897108E-4
76-77	3.9605889712647385E-5	0.0	0.0	6.351022247816428	5.940883456897108E-4
78-79	3.9605889712647385E-5	0.0	0.0	6.996934900195138	5.940883456897108E-4
80-81	3.9605889712647385E-5	0.0	0.0	7.656372963910718	6.138912905460345E-4
82-83	3.9605889712647385E-5	0.0	0.0	8.335475351868626	7.525119045403004E-4
84-85	3.9605889712647385E-5	0.0	0.0	9.047767475405733	7.525119045403004E-4
86-87	3.9605889712647385E-5	0.0	0.0	9.810161049429338	7.723148493966241E-4
88-89	3.9605889712647385E-5	0.0	0.0	10.638676656328208	7.921177942529478E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1855	0.0	51.4358	1
GTATCAA	3175	0.0	38.573524	1
TCAACGC	3925	0.0	30.11551	4
CAACGCA	3990	0.0	29.742706	5
ATCAACG	4070	0.0	29.158081	3
TATCAAC	4160	0.0	28.414276	2
GTACATG	6150	0.0	28.404428	1
AACGCAG	4295	0.0	27.943327	6
TACATGG	6400	0.0	26.776745	2
ACATGGG	6795	0.0	24.520594	3
ACGCAGA	5130	0.0	22.842937	7
GTATAGA	740	0.0	22.45172	1
CATGGGG	5760	0.0	22.004896	4
GTATTAG	645	0.0	20.606852	1
CGCAGAG	5625	0.0	20.411045	8
GAGTACA	4760	0.0	19.945107	1
TAAGGCG	840	0.0	18.648676	5
GTATTAA	1295	0.0	18.327936	1
TAAGGTG	1145	0.0	18.241499	5
CATACCG	210	1.9682044E-5	18.083565	5
>>END_MODULE