FastQCFastQC Report
Mon 15 Aug 2016
SRR2921647_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921647_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2524877
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16850.051.056711
GTATCAA32900.040.1627161
TCAACGC41850.031.416984
ATCAACG43450.030.3705313
TATCAAC43250.030.291472
CAACGCA43550.030.1042735
AACGCAG46250.028.3412136
GTACATG63500.027.545351
TACATGG67000.025.646682
ACATGGG71500.024.2317183
ACGCAGA56600.022.8225927
CATGGGG60400.022.4754944
CGCAGAG58700.021.8443018
GTATAGG7200.019.144311
TATAGGG3254.9294613E-1018.9870432
GTATTAC4700.018.2032451
GCAGAGT66650.018.0986469
ATGGGGG34750.017.770345
AGAGTAC56300.017.33468410-11
TAAGGTG11900.017.1644025
Produced by FastQC (version 0.11.5)