##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921647_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2524877
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.96102820058165	33.0	31.0	34.0	28.0	34.0
2	31.102480239631475	33.0	31.0	34.0	28.0	34.0
3	31.171649945720127	34.0	31.0	34.0	27.0	34.0
4	34.65366233681878	37.0	35.0	37.0	32.0	37.0
5	34.35344810856133	37.0	35.0	37.0	30.0	37.0
6	34.3157282513168	37.0	35.0	37.0	30.0	37.0
7	34.17977945064255	37.0	35.0	37.0	30.0	37.0
8	34.11662627526014	37.0	35.0	37.0	30.0	37.0
9	35.6573678638603	39.0	37.0	39.0	30.0	39.0
10-11	35.53612413594801	39.0	37.0	39.0	30.0	39.0
12-13	35.44014361887727	39.0	37.0	39.0	30.0	39.0
14-15	36.62162196415905	40.0	37.0	41.0	30.0	41.0
16-17	36.57284671688957	40.0	37.0	41.0	30.0	41.0
18-19	36.5268422976644	40.0	37.0	41.0	29.0	41.0
20-21	36.45690938608099	40.0	37.0	41.0	29.0	41.0
22-23	36.35774138700618	40.0	37.0	41.0	28.5	41.0
24-25	36.263328273020825	40.0	37.0	41.0	28.0	41.0
26-27	36.123891777698475	40.0	36.0	41.0	27.0	41.0
28-29	35.989469982102094	40.0	36.0	41.0	27.0	41.0
30-31	35.82198538780305	40.0	36.0	41.0	27.0	41.0
32-33	35.763506301495084	40.0	36.0	41.0	27.0	41.0
34-35	35.892079297328145	40.0	36.0	41.0	27.0	41.0
36-37	35.880525269151725	40.0	36.0	41.0	27.0	41.0
38-39	35.76203137816218	40.0	36.0	41.0	26.0	41.0
40-41	35.6694282533367	40.0	36.0	41.0	26.0	41.0
42-43	35.53498051588255	40.0	35.0	41.0	25.5	41.0
44-45	35.39007127871972	40.0	35.0	41.0	25.0	41.0
46-47	35.219989132143866	40.0	35.0	41.0	24.0	41.0
48-49	35.06613213237714	39.0	35.0	41.0	24.0	41.0
50-51	34.894506742308636	39.0	35.0	41.0	23.5	41.0
52-53	34.70766377926529	39.0	35.0	41.0	23.0	41.0
54-55	34.48153335786258	39.0	34.5	41.0	22.5	41.0
56-57	34.23881539575987	38.0	34.0	40.0	22.0	41.0
58-59	33.98691956083405	38.0	34.0	40.0	21.0	41.0
60-61	33.70125237783861	37.5	34.0	40.0	20.0	41.0
62-63	33.39053130112873	37.0	33.5	40.0	18.5	41.0
64-65	33.04748251102925	36.5	33.0	39.5	18.0	41.0
66-67	32.69198816417592	36.0	33.0	39.0	14.0	41.0
68-69	32.32942674039171	35.0	33.0	39.0	12.5	40.5
70-71	31.936890985184625	35.0	32.0	38.0	8.0	40.0
72-73	31.579115537113292	35.0	32.0	37.0	7.0	39.5
74-75	31.185372594387765	35.0	32.0	37.0	2.0	39.0
76-77	28.530393955824383	32.0	27.5	34.5	2.0	36.0
78-79	30.082003796620587	34.5	30.5	35.0	2.0	37.0
80-81	30.271012409713425	35.0	31.0	35.0	2.0	37.0
82-83	30.106550140858346	35.0	31.0	35.0	2.0	36.5
84-85	29.91508556654443	35.0	31.0	35.0	2.0	36.0
86-87	29.709754574183215	34.5	31.0	35.0	2.0	36.0
88-89	29.440679486565088	34.0	31.0	35.0	2.0	35.5
90-91	29.236961444062423	34.0	31.0	35.0	2.0	35.0
92-93	29.070787210624516	34.0	30.0	35.0	2.0	35.0
94-95	28.959490105854663	34.0	30.0	35.0	2.0	35.0
96-97	28.736058429776975	34.0	30.0	35.0	2.0	35.0
98-99	28.516612096351622	34.0	30.0	35.0	2.0	35.0
100-101	27.38066388976572	33.0	27.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	67702.0
3	21746.0
4	11500.0
5	8119.0
6	6922.0
7	6933.0
8	7075.0
9	7613.0
10	8142.0
11	8632.0
12	9056.0
13	9694.0
14	10231.0
15	10822.0
16	11610.0
17	12141.0
18	11583.0
19	10422.0
20	10288.0
21	10324.0
22	10770.0
23	12153.0
24	14135.0
25	17153.0
26	20184.0
27	24469.0
28	29250.0
29	34910.0
30	42591.0
31	53387.0
32	66539.0
33	87505.0
34	124462.0
35	186412.0
36	327431.0
37	598026.0
38	557475.0
39	57470.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.51800491279258	19.59659253847316	13.10124295318092	26.78415959555334
2	15.388706987406486	21.08052252966587	36.51964793448854	27.011122548439108
3	17.915067274485207	24.315025763895846	28.864911154267688	28.90499580735126
4	13.386382225320482	16.512873730302232	34.21229722958829	35.88844681478899
5	14.39814158233299	37.06424518816716	34.04451455414535	14.493098675354494
6	34.632738466315644	34.99494221770881	16.24296683092907	14.12935248504647
7	29.385550266409016	31.424738710044092	20.625836426883367	18.56387459666352
8	26.936797317255458	34.82601330678683	19.48288966155579	18.754299714401927
9	26.787324689479924	15.113488696677106	18.886662597821598	39.212524016021376
10-11	26.03675191712998	25.012842943236073	27.51967847966496	21.430726659968986
12-13	26.35309414878902	22.406772941424123	27.289320382966174	23.950812526820684
14-15	23.532468550952498	23.99716646166356	24.3808071148191	28.08955787256484
16-17	24.024556443739638	27.070823648043053	25.19285097848331	23.711768929734003
18-19	23.971438959648104	26.469440811648116	26.448092907346926	23.111027321356854
20-21	24.409865773313918	25.701417161791067	26.333865439831467	23.554851625063545
22-23	24.28448989792374	25.443100792632674	26.12515381937417	24.147255490069416
24-25	24.139819835804985	25.61512731282305	26.062577244274788	24.182475607097174
26-27	23.91246616324855	25.853882267809436	26.15034063006413	24.08331093887789
28-29	24.05700125339068	26.075298895752713	25.923204719355546	23.94449513150106
30-31	24.337919810622875	25.802098763451497	25.714120475589787	24.14586095033584
32-33	24.105603012905778	25.86746209111442	25.63221746284371	24.3947174331361
34-35	24.35863589975357	25.888359858252976	25.799297602409215	23.95370663958424
36-37	24.41412438687436	25.718759109747907	25.88109085666485	23.986025646712886
38-39	24.256013263220346	25.78872158921009	25.82169349239587	24.133571655173697
40-41	24.345899980394524	25.67614132576575	25.871958428472695	24.10600026536703
42-43	24.31095617703467	25.60641877038395	25.661020225313685	24.421604827267693
44-45	24.0951340569277	25.692386178061565	25.820796914973638	24.391682850037093
46-47	24.265023611687063	25.69653783711956	25.703944337967744	24.334494213225636
48-49	24.017717874964053	25.78237576059084	25.76774025402011	24.432166110424998
50-51	24.135047936052523	25.79005569501533	25.81007690839196	24.26481946054019
52-53	24.368308079782906	25.651386342784342	25.729787769575452	24.250517807857292
54-55	23.84756204307864	25.539294939990764	25.9985828456997	24.614560171230895
56-57	23.970026252873637	25.639943501661204	25.797211206507292	24.59281903895787
58-59	23.964812118650563	25.51289747635725	25.892278705671266	24.630011699320917
60-61	23.870471354432272	25.559231470763272	26.022971114304017	24.54732606050044
62-63	23.883845673448022	25.61526271035873	25.966561273136413	24.53433034305683
64-65	23.744008368225707	25.459890860427542	25.832161999657266	24.963938771689484
66-67	23.77142146085209	25.535546428554447	25.73779307633657	24.955239034256902
68-69	23.83820892565258	25.495289514091834	25.782099162910495	24.88440239734509
70-71	23.69783265389917	25.32793960632187	25.788767181704976	25.185460558073984
72-73	23.697595950323556	25.495418381583434	25.6704146722722	25.13657099582081
74-75	23.672307811767094	25.590188860435337	25.631984413230057	25.105518914567515
76-77	23.667336311842043	25.221041451386956	25.79669286703855	25.314929369732447
78-79	23.576052443769548	25.325994820549504	25.799684444743047	25.2982682909379
80-81	23.80163710539728	25.344835239220775	25.607890508767717	25.245637146614225
82-83	23.62045479170616	25.38569980593932	25.668362444415788	25.32548295793873
84-85	23.547237564025448	25.352095374634555	25.678929278183677	25.421737783156324
86-87	23.553226222592656	25.432241735934337	25.556132868682134	25.458399172790873
88-89	23.3951217962212	25.21378384497146	25.606300119552138	25.784794239255206
90-91	23.29197086090611	25.18366844350527	25.71393957689527	25.81042111869335
92-93	23.369554341914565	25.007434734325884	25.805908360088804	25.817102563670748
94-95	23.291943917526424	24.875695099378564	25.834743105846027	25.997617877248985
96-97	23.194597497993687	24.752053250223604	25.8911941768263	26.162155074956406
98-99	23.03234923565787	24.706150976829917	25.829662819706982	26.43183696780523
100-101	22.97810327677543	24.752606613672633	25.784598092064343	26.484692017487593
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	38.0
1	32.5
2	54.0
3	91.0
4	86.5
5	92.5
6	150.0
7	309.5
8	446.0
9	439.5
10	373.5
11	333.5
12	347.5
13	355.0
14	386.5
15	431.0
16	443.0
17	503.5
18	606.0
19	678.5
20	762.5
21	851.0
22	935.5
23	1125.5
24	1409.0
25	1713.5
26	2210.5
27	2960.0
28	4016.0
29	5779.5
30	8495.5
31	12261.0
32	17621.5
33	22243.5
34	26061.5
35	31098.0
36	37518.5
37	44197.5
38	51111.0
39	58012.0
40	64482.0
41	70870.5
42	76900.5
43	83434.5
44	89891.5
45	96599.5
46	101605.0
47	105610.0
48	109909.5
49	112252.5
50	112650.0
51	113129.5
52	113223.0
53	112912.0
54	112028.0
55	106859.5
56	98091.5
57	88153.5
58	79459.0
59	69739.0
60	58125.0
61	48361.0
62	40122.0
63	31137.5
64	22687.5
65	16774.0
66	12757.0
67	10237.0
68	8012.0
69	5998.5
70	4524.0
71	3329.5
72	2393.5
73	1736.0
74	1333.5
75	1075.5
76	750.5
77	445.0
78	270.5
79	164.5
80	97.0
81	60.0
82	34.0
83	21.5
84	19.0
85	17.0
86	10.0
87	6.5
88	4.0
89	1.5
90	2.0
91	2.0
92	1.0
93	1.0
94	0.5
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14670021549564594
2	2.376353382758843E-4
3	0.008713295736782424
4	0.006416154133448877
5	0.022931810143622836
6	0.002178323934195606
7	0.0
8	0.0
9	0.0
10-11	0.04425958175388346
12-13	2.57438283132208E-4
14-15	0.004693297930948716
16-17	0.0
18-19	0.0015446296987932481
20-21	2.57438283132208E-4
22-23	0.0
24-25	5.346795111207397E-4
26-27	0.002792215224741641
28-29	0.004970539158937247
30-31	0.015466099932788805
32-33	0.010733196112127443
34-35	0.009446004696466403
36-37	0.004317041978678566
38-39	0.0
40-41	0.002851624059310612
42-43	0.008673689847069777
44-45	0.006574577692299466
46-47	0.0026932005004600226
48-49	0.00772314849396624
50-51	0.002178323934195606
52-53	0.002000097430488693
54-55	0.003920983081552092
56-57	0.007960783832242125
58-59	0.014713588028248504
60-61	0.0073072866519834434
62-63	0.020931712713134144
64-65	0.0413683518048602
66-67	0.0583394755467296
68-69	0.049586573920234536
70-71	0.06729040662178791
72-73	0.032615450178365125
74-75	0.02713003445316346
76-77	0.21321830726803723
78-79	0.08010291194382935
80-81	0.06507247679787967
82-83	0.026397325493479482
84-85	0.05033908582477483
86-87	0.06709237717322467
88-89	0.2830632937762909
90-91	0.289004177233188
92-93	0.049487559195952914
94-95	0.009426201751610078
96-97	0.013584820171438054
98-99	0.001029753132528832
100-101	5.940883456897108E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2524877.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.588776875811725
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.30555277833655	48.8433199507788
2	11.40802124908041	14.052121506082646
3	3.7485110327236915	6.92598628832814
4	1.7098446760326662	4.212289689778819
5	0.955878989116222	2.943570889047773
6	0.6337058830202681	2.341750214053472
7	0.421762064765585	1.8183066791070492
8	0.32734347660987806	1.6128547474142618
9	0.2474529994221768	1.3716294811796532
>10	1.1925289649472874	12.458880053231923
>50	0.037892412210278434	1.5445048052380104
>100	0.010086887496457558	1.1313152927404855
>500	0.001096180275277503	0.4877969638536183
>1k	3.2240596331401303E-4	0.25567343916540325
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.9605889712647385E-5
2	0.0	0.0	0.0	0.0	3.9605889712647385E-5
3	0.0	0.0	0.0	0.0	3.9605889712647385E-5
4	0.0	0.0	0.0	0.0	3.9605889712647385E-5
5	0.0	0.0	0.0	0.0	3.9605889712647385E-5
6	0.0	0.0	0.0	0.0	7.921177942529477E-5
7	0.0	0.0	3.9605889712647385E-5	3.9605889712647385E-5	7.921177942529477E-5
8	0.0	0.0	3.9605889712647385E-5	3.9605889712647385E-5	1.1881766913794217E-4
9	0.0	0.0	3.9605889712647385E-5	3.9605889712647385E-5	1.1881766913794217E-4
10-11	0.0	0.0	3.9605889712647385E-5	3.9605889712647385E-5	1.1881766913794217E-4
12-13	0.0	0.0	3.9605889712647385E-5	3.9605889712647385E-5	1.5842355885058954E-4
14-15	0.0	0.0	3.9605889712647385E-5	3.9605889712647385E-5	2.1783239341956065E-4
16-17	0.0	0.0	3.9605889712647385E-5	5.940883456897108E-5	2.772412279885317E-4
18-19	0.0	0.0	3.9605889712647385E-5	7.921177942529477E-5	3.564530074138265E-4
20-21	0.0	0.0	3.9605889712647385E-5	7.921177942529477E-5	3.564530074138265E-4
22-23	0.0	0.0	3.9605889712647385E-5	7.921177942529477E-5	3.762559522701502E-4
24-25	0.0	0.0	3.9605889712647385E-5	2.57438283132208E-4	3.960588971264739E-4
26-27	0.0	0.0	3.9605889712647385E-5	6.53497180258682E-4	3.960588971264739E-4
28-29	0.0	0.0	3.9605889712647385E-5	0.0032476829564370858	3.960588971264739E-4
30-31	0.0	0.0	3.9605889712647385E-5	0.015683932326208364	3.960588971264739E-4
32-33	0.0	0.0	3.9605889712647385E-5	0.048695441401699964	4.3566478683912125E-4
34-35	0.0	0.0	3.9605889712647385E-5	0.09345009677699151	4.3566478683912125E-4
36-37	1.9802944856323693E-5	0.0	3.9605889712647385E-5	0.16119597113047487	4.3566478683912125E-4
38-39	3.9605889712647385E-5	0.0	3.9605889712647385E-5	0.2734390625761176	4.3566478683912125E-4
40-41	3.9605889712647385E-5	0.0	3.9605889712647385E-5	0.39393998202684727	4.3566478683912125E-4
42-43	3.9605889712647385E-5	0.0	3.9605889712647385E-5	0.5277484804210265	4.3566478683912125E-4
44-45	3.9605889712647385E-5	0.0	3.9605889712647385E-5	0.6966676000454676	4.55467731695445E-4
46-47	5.940883456897108E-5	0.0	3.9605889712647385E-5	0.8903998095748823	4.752706765517687E-4
48-49	7.921177942529477E-5	0.0	3.9605889712647385E-5	1.0902115231751883	5.14876566264416E-4
50-51	7.921177942529477E-5	0.0	3.9605889712647385E-5	1.2949343670998625	5.14876566264416E-4
52-53	7.921177942529477E-5	0.0	3.9605889712647385E-5	1.5037762235546523	5.544824559770634E-4
54-55	7.921177942529477E-5	0.0	3.9605889712647385E-5	1.7572737206604518	6.138912905460345E-4
56-57	7.921177942529477E-5	0.0	3.9605889712647385E-5	2.0449114946985536	6.336942354023582E-4
58-59	7.921177942529477E-5	0.0	3.9605889712647385E-5	2.34496571516157	6.336942354023582E-4
60-61	7.921177942529477E-5	0.0	3.9605889712647385E-5	2.6622484976495886	6.336942354023582E-4
62-63	7.921177942529477E-5	0.0	3.9605889712647385E-5	2.9880069405361134	7.12906014827653E-4
64-65	7.921177942529477E-5	3.9605889712647385E-5	3.9605889712647385E-5	3.3472521631746814	7.12906014827653E-4
66-67	7.921177942529477E-5	3.9605889712647385E-5	3.9605889712647385E-5	3.771569862611129	7.12906014827653E-4
68-69	7.921177942529477E-5	3.9605889712647385E-5	3.9605889712647385E-5	4.218462919183786	7.12906014827653E-4
70-71	7.921177942529477E-5	3.9605889712647385E-5	3.9605889712647385E-5	4.678346707582191	7.12906014827653E-4
72-73	7.921177942529477E-5	3.9605889712647385E-5	3.9605889712647385E-5	5.160370188329966	7.12906014827653E-4
74-75	7.921177942529477E-5	3.9605889712647385E-5	3.9605889712647385E-5	5.679801431911336	7.12906014827653E-4
76-77	7.921177942529477E-5	3.9605889712647385E-5	3.9605889712647385E-5	6.268265741261851	7.12906014827653E-4
78-79	7.921177942529477E-5	7.921177942529477E-5	3.9605889712647385E-5	6.9095247015993255	7.12906014827653E-4
80-81	7.921177942529477E-5	7.921177942529477E-5	3.9605889712647385E-5	7.565418038185623	7.12906014827653E-4
82-83	7.921177942529477E-5	7.921177942529477E-5	3.9605889712647385E-5	8.240619246006835	7.327089596839767E-4
84-85	7.921177942529477E-5	7.921177942529477E-5	3.9605889712647385E-5	8.948970583517532	7.525119045403004E-4
86-87	7.921177942529477E-5	7.921177942529477E-5	3.9605889712647385E-5	9.706472830161628	7.525119045403004E-4
88-89	7.921177942529477E-5	7.921177942529477E-5	3.9605889712647385E-5	10.529384203666158	7.525119045403004E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1685	0.0	51.05671	1
GTATCAA	3290	0.0	40.162716	1
TCAACGC	4185	0.0	31.41698	4
ATCAACG	4345	0.0	30.370531	3
TATCAAC	4325	0.0	30.29147	2
CAACGCA	4355	0.0	30.104273	5
AACGCAG	4625	0.0	28.341213	6
GTACATG	6350	0.0	27.54535	1
TACATGG	6700	0.0	25.64668	2
ACATGGG	7150	0.0	24.231718	3
ACGCAGA	5660	0.0	22.822592	7
CATGGGG	6040	0.0	22.475494	4
CGCAGAG	5870	0.0	21.844301	8
GTATAGG	720	0.0	19.14431	1
TATAGGG	325	4.9294613E-10	18.987043	2
GTATTAC	470	0.0	18.203245	1
GCAGAGT	6665	0.0	18.098646	9
ATGGGGG	3475	0.0	17.77034	5
AGAGTAC	5630	0.0	17.334684	10-11
TAAGGTG	1190	0.0	17.164402	5
>>END_MODULE