FastQCFastQC Report
Mon 15 Aug 2016
SRR2921646_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921646_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2577242
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23900.040.715951
GGTATCA14850.035.16211
CAACGCA28250.033.1028065
TCAACGC28350.032.8198744
ATCAACG30500.030.3495183
TATCAAC32950.029.1007752
AACGCAG32850.028.7469316
GTACATG64050.027.5697251
TACATGG66450.025.4310132
ACATGGG71800.023.0737423
ACGCAGA39650.022.9821577
GTATACG1050.002205351422.6042081
CGCAGAG44400.020.3116658
TATACCG2351.14087015E-720.1998985
GAGTACA48350.019.930041
CATGGGG63400.019.9171094
AGTACAT47550.019.3668882
ATGGGGG33650.018.6211245
GTCTTAG8700.018.005421
GTATAAG8350.017.0546721
Produced by FastQC (version 0.11.5)