##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921646_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2577242
Sequences flagged as poor quality	0
Sequence length	101
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.918649859035355	33.0	31.0	34.0	28.0	34.0
2	30.984897033340292	33.0	31.0	34.0	28.0	34.0
3	31.049823415884113	34.0	31.0	34.0	27.0	34.0
4	34.46487407856926	37.0	35.0	37.0	32.0	37.0
5	34.45772379931726	37.0	35.0	37.0	32.0	37.0
6	34.50463518753769	37.0	35.0	37.0	32.0	37.0
7	34.43287475526163	37.0	35.0	37.0	32.0	37.0
8	34.40494101834442	37.0	35.0	37.0	31.0	37.0
9	36.03807131809896	39.0	37.0	39.0	32.0	39.0
10-11	35.94931694423729	39.0	37.0	39.0	31.0	39.0
12-13	35.853755875466874	39.0	37.0	39.0	31.0	39.0
14-15	37.107956489922174	40.0	38.0	41.0	31.0	41.0
16-17	37.05001955578871	40.0	38.0	41.0	31.0	41.0
18-19	36.99953108788387	40.0	38.0	41.0	31.0	41.0
20-21	36.926102205380786	40.0	38.0	41.0	30.5	41.0
22-23	36.83181885907493	40.0	38.0	41.0	30.0	41.0
24-25	36.7344504318958	40.0	38.0	41.0	30.0	41.0
26-27	36.599003508401616	40.0	37.0	41.0	30.0	41.0
28-29	36.472415667601254	40.0	37.0	41.0	30.0	41.0
30-31	36.34787575245165	40.0	37.0	41.0	29.5	41.0
32-33	36.19581475080726	40.0	37.0	41.0	29.0	41.0
34-35	36.039314701529776	40.0	36.0	41.0	28.0	41.0
36-37	36.02751022216773	40.0	36.5	41.0	28.0	41.0
38-39	36.22882018840295	40.0	37.0	41.0	29.0	41.0
40-41	36.29939699104702	40.0	37.0	41.0	29.0	41.0
42-43	36.165844922595554	40.0	37.0	41.0	28.0	41.0
44-45	36.05634705627178	40.0	37.0	41.0	28.0	41.0
46-47	35.91253479494746	40.0	36.0	41.0	27.5	41.0
48-49	35.75783686592101	40.0	36.0	41.0	27.0	41.0
50-51	34.07720384814465	38.0	33.5	39.5	24.5	40.0
52-53	34.207306686760504	38.5	34.0	39.5	23.5	40.0
54-55	35.09896121512842	39.0	35.0	40.5	26.0	41.0
56-57	35.09227965398671	39.0	35.0	41.0	26.0	41.0
58-59	34.88248852843466	39.0	35.0	41.0	26.0	41.0
60-61	34.618253155893	38.0	35.0	41.0	25.5	41.0
62-63	34.313175867846326	37.5	34.5	40.0	25.0	41.0
64-65	33.99996721301298	37.0	34.0	40.0	24.0	41.0
66-67	33.64456830208417	36.5	34.0	39.5	23.0	41.0
68-69	33.27375911924453	36.0	34.0	39.0	22.5	41.0
70-71	32.864272738066504	35.5	34.0	39.0	22.0	40.5
72-73	32.4979157952571	35.0	33.5	37.5	20.5	39.5
74-75	32.1044963957595	35.0	33.0	37.0	20.0	39.0
76-77	31.747175274964476	35.0	33.0	36.5	18.0	39.0
78-79	31.43229021566465	35.0	33.0	36.0	17.5	37.5
80-81	31.139083368965743	35.0	33.0	35.5	14.0	37.0
82-83	30.866339288277935	35.0	32.5	35.0	9.0	37.0
84-85	30.637517741834102	35.0	32.0	35.0	7.0	36.0
86-87	30.437531865459277	35.0	32.0	35.0	2.0	36.0
88-89	30.25333573641901	35.0	32.0	35.0	2.0	36.0
90-91	30.061516923905476	35.0	32.0	35.0	2.0	35.0
92-93	29.904736536188686	35.0	32.0	35.0	2.0	35.0
94-95	29.710860485744064	35.0	32.0	35.0	2.0	35.0
96-97	29.51577015274468	35.0	31.0	35.0	2.0	35.0
98-99	29.288051335497407	35.0	31.0	35.0	2.0	35.0
100-101	28.264935539619486	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	77600.0
3	13336.0
4	8954.0
5	6762.0
6	6122.0
7	6075.0
8	6305.0
9	6833.0
10	7095.0
11	7430.0
12	7380.0
13	7383.0
14	7637.0
15	7979.0
16	7813.0
17	8079.0
18	8060.0
19	7848.0
20	7993.0
21	8490.0
22	9225.0
23	10854.0
24	12713.0
25	15231.0
26	18074.0
27	21569.0
28	26077.0
29	30807.0
30	36951.0
31	45674.0
32	58134.0
33	77397.0
34	110389.0
35	171850.0
36	317485.0
37	631137.0
38	662454.0
39	96035.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.255787523124084	16.589722231494715	11.761048131480663	26.393442113900534
2	16.886540769946286	18.46600835135962	37.922983631148796	26.72446724754529
3	20.162602067871592	21.91645749077439	27.718089645036336	30.202850796317676
4	13.789066109099773	14.416600606998417	34.692040308173205	37.1022929757286
5	15.454039040467071	35.68718714152897	33.53540019681333	15.323373621190623
6	35.71326017446373	33.276006581193926	16.273825784621117	14.73690745972123
7	30.968637453526227	29.778062864790755	19.953620326962724	19.299679354720293
8	27.747171192202934	33.48678234002815	19.16837183528401	19.597674632484907
9	26.87122739541475	14.897801121556832	18.258824150256952	39.97214733277147
10-11	26.581983266400748	24.104287553100132	26.969182035808707	22.344547144690413
12-13	26.882441502689474	21.300978556209014	26.917174417248635	24.899405523852877
14-15	24.040807153827554	22.818622494625277	23.76631678718962	29.37425356435755
16-17	24.782807889258073	25.884685159488313	24.57389674014391	24.7586102111097
18-19	24.70880249403286	25.394378259322455	25.627340144530663	24.269479102114023
20-21	25.088898504549224	24.610594862262133	25.571490386387847	24.729016246800796
22-23	24.908855250669166	24.41600312978659	25.37145354407385	25.303688075470394
24-25	24.774480628669508	24.643142338454194	25.32874839538492	25.253628637491378
26-27	24.69947374068675	24.833711487417492	25.334784244547876	25.132030527347887
28-29	24.770793508875276	24.969518039654876	25.214848343030905	25.044840108438944
30-31	24.85946466573429	24.788702224195283	25.117601085321617	25.23423202474881
32-33	24.624924282492948	24.85916834722991	25.143412159736872	25.372495210540265
34-35	24.83987853041357	24.850355398652596	25.256528058993304	25.053238011940525
36-37	24.899499598114797	24.78026298405438	25.2528248853371	25.067412532493723
38-39	24.832406954890683	24.878911805887938	25.231607544844586	25.05707369437679
40-41	24.91659121381537	24.749744540282123	25.285380564651827	25.048283681250684
42-43	24.773533537478137	24.700588592257773	25.226923097101693	25.298954773162404
44-45	24.70931401546168	24.73674446073046	25.354628839072458	25.199312684735403
46-47	24.902656834109994	24.73738424410375	25.233940271842037	25.12601864994422
48-49	24.715668327575266	24.766766233105148	25.209141978736277	25.308423460583306
50-51	24.614408759450242	24.864440086219037	25.362831978754137	25.158319175576583
52-53	24.885954400411574	24.77284137131382	25.29076770757076	25.05043652070384
54-55	24.574088328514527	24.61554781109863	25.509102629510373	25.301261230876477
56-57	24.61060400530476	24.7574058581633	25.471714609115782	25.16027552741616
58-59	24.695392015500428	24.67609808776386	25.401650806332682	25.226859090403032
60-61	24.60252137056049	24.698235677925826	25.50813493890878	25.191108012604914
62-63	24.6490936477533	24.679712409010698	25.49921112005022	25.171982823185786
64-65	24.534556628870632	24.572577381557196	25.548255562405032	25.34461042716714
66-67	24.505183917054218	24.73548093298462	25.410382910073036	25.348952239888128
68-69	24.65161141800316	24.62552100686063	25.438655965000383	25.284211610135827
70-71	24.53386829387501	24.501174677930003	25.529984300071057	25.434972728123928
72-73	24.522706043806604	24.519092535346328	25.51111717248014	25.447084248366924
74-75	24.47147540830735	24.655997612751264	25.561570735065903	25.310956243875488
76-77	24.501658063901996	24.459871356776112	25.551260109138873	25.48721047018302
78-79	24.487413215273918	24.424500178460853	25.593209899690816	25.49487670657441
80-81	24.50783922615271	24.583778237876817	25.44679751313339	25.461585022837085
82-83	24.446270540667843	24.60642207254192	25.528682707987134	25.418624678803102
84-85	24.428757956585603	24.578485547964902	25.581928544226567	25.410827951222924
86-87	24.37069570788753	24.61452178936901	25.589554053037762	25.425228449705696
88-89	24.36562407698167	24.39420849720957	25.620248884605218	25.61991854120354
90-91	24.29536354654837	24.42018097458256	25.697504584217857	25.586950894651217
92-93	24.32819552970471	24.46892337755385	25.730888013706682	25.471993079034753
94-95	24.305958521331846	24.23252104501587	25.812524752840822	25.64899568081146
96-97	24.29416312285812	24.2748844934132	25.800208877731123	25.63074350599756
98-99	24.288918504734067	24.17671254013394	25.779388382271378	25.754980572860614
100-101	24.45380739761834	23.986726459142076	25.901556191484914	25.657909951754664
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9.0
1	10.0
2	20.0
3	30.0
4	27.0
5	25.5
6	57.5
7	123.0
8	173.5
9	205.5
10	208.5
11	192.0
12	216.5
13	259.5
14	309.0
15	368.5
16	431.0
17	439.0
18	477.5
19	575.0
20	636.0
21	664.0
22	694.5
23	758.5
24	849.5
25	985.0
26	1136.0
27	1351.5
28	1651.5
29	1986.0
30	2485.5
31	3413.0
32	5391.0
33	8122.0
34	11763.5
35	17401.0
36	25508.5
37	34233.5
38	42696.5
39	50224.0
40	56801.0
41	63784.0
42	71639.5
43	81285.5
44	90403.0
45	99271.0
46	106394.5
47	111894.5
48	118109.0
49	121807.5
50	122046.5
51	122734.5
52	123571.0
53	123360.5
54	122686.5
55	118050.0
56	109586.5
57	99768.0
58	91432.5
59	82162.5
60	69471.0
61	57419.5
62	47040.0
63	36569.0
64	26897.5
65	19963.0
66	15324.5
67	12227.5
68	9684.0
69	7412.0
70	5674.5
71	4203.0
72	3013.5
73	2250.5
74	1815.0
75	1418.0
76	1065.0
77	747.5
78	511.0
79	358.0
80	254.0
81	179.5
82	140.5
83	125.5
84	95.5
85	71.5
86	69.0
87	56.0
88	50.0
89	44.5
90	36.0
91	37.5
92	27.5
93	17.0
94	17.5
95	17.0
96	13.0
97	8.5
98	9.5
99	9.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03585227929701596
2	0.05331280492867957
3	0.04888947176865812
4	0.04997590447462831
5	0.04644499818022522
6	0.007837835950213446
7	0.009739093185661261
8	0.01059271888320926
9	0.004268128487739995
10-11	0.010515116547068533
12-13	0.016897908694643343
14-15	0.032243770666472146
16-17	0.02128244068659443
18-19	0.008187046462846717
20-21	0.01656809876604525
22-23	0.027490627577852606
24-25	0.026753405384515695
26-27	0.018255949577106072
28-29	0.011950759765671986
30-31	0.012028362101812714
32-33	0.008885467488113263
34-35	0.005276958857569449
36-37	5.238157689499085E-4
38-39	0.0035503068784382683
40-41	9.894297857942717E-4
42-43	0.15786255229427426
44-45	0.13423264093942283
46-47	0.14026622257436439
48-49	0.14554318143193382
50-51	0.13830676358681102
52-53	0.14395233354104892
54-55	0.1414302576164753
56-57	0.1437583277006971
58-59	0.15107234788196064
60-61	0.15361382439056945
62-63	0.14187647104928447
64-65	0.14152726053665118
66-67	0.1400334155659422
68-69	0.1357846876622374
70-71	0.1297899071953662
72-73	0.13836496533891657
74-75	0.1384425676750573
76-77	0.1341356380192469
78-79	0.1493262953187943
80-81	0.16003541770621463
82-83	0.157299935357254
84-85	0.1514021578105587
86-87	0.16690322445466899
88-89	0.16149046150885327
90-91	0.16622420401343763
92-93	0.16259629479885862
94-95	0.16703902854291527
96-97	0.17247119207276615
98-99	0.16665101686221162
100-101	0.17283980316943462
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2577242.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.67081541469371
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.41189509652386	46.789017186210025
2	11.7938660939978	14.074992134411149
3	3.9647230766572172	7.097347766327681
4	1.8339203873980727	4.377260996866959
5	1.0024205628160177	2.9907626185843985
6	0.6294979439764465	2.2537593371368647
7	0.4561736924384046	1.905417933897191
8	0.34499219346138027	1.646877239643546
9	0.24673809857029355	1.325075718200444
>10	1.2365675027012395	13.028392347278
>50	0.06019064039454883	2.42259001711693
>100	0.018363497145624217	1.8246188646937587
>500	5.209711352993518E-4	0.17676715984696545
>1k	1.302427838138505E-4	0.0871206797861044
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.880116807036359E-5
2	0.0	0.0	0.0	0.0	7.760233614072718E-5
3	0.0	0.0	0.0	0.0	7.760233614072718E-5
4	0.0	0.0	0.0	0.0	7.760233614072718E-5
5	0.0	0.0	0.0	0.0	1.1640350421109077E-4
6	0.0	0.0	0.0	0.0	2.3280700842218154E-4
7	0.0	0.0	0.0	0.0	2.7160817649254514E-4
8	0.0	0.0	0.0	0.0	3.1040934456290874E-4
9	0.0	0.0	0.0	0.0	3.880116807036359E-4
10-11	0.0	0.0	0.0	0.0	3.880116807036359E-4
12-13	0.0	0.0	0.0	3.880116807036359E-5	5.238157689499085E-4
14-15	0.0	0.0	0.0	3.880116807036359E-5	5.626169370202721E-4
16-17	0.0	0.0	0.0	3.880116807036359E-5	6.208186891258175E-4
18-19	0.0	0.0	0.0	1.7460525631663614E-4	6.984210252665447E-4
20-21	0.0	0.0	0.0	2.5220759245736334E-4	7.372221933369082E-4
22-23	0.0	0.0	0.0	3.1040934456290874E-4	9.118274496535444E-4
24-25	0.0	0.0	0.0	4.462134328091813E-4	0.0010088303698294534
26-27	0.0	0.0	0.0	5.626169370202721E-4	0.0010864327059701806
28-29	0.0	0.0	0.0	0.0011640350421109075	0.0011252338740405442
30-31	0.0	0.0	0.0	0.004694941336513995	0.0011252338740405442
32-33	0.0	0.0	0.0	0.014511636858315982	0.0011834356261460894
34-35	0.0	0.0	0.0	0.029915700582250328	0.0012804385463219986
36-37	0.0	0.0	0.0	0.057658535752560296	0.0012804385463219986
38-39	0.0	0.0	0.0	0.10802245190789224	0.0013192397143923622
40-41	0.0	0.0	0.0	0.1669614262067745	0.0013192397143923622
42-43	0.0	0.0	0.0	0.23162357279603546	0.0013774414664979074
44-45	0.0	0.0	0.0	0.3129508210715175	0.0014744443866738163
46-47	0.0	0.0	0.0	0.4116415920584873	0.0015520467228145435
48-49	0.0	0.0	0.0	0.5177433861468965	0.0015520467228145435
50-51	0.0	0.0	0.0	0.6344961008706206	0.0015520467228145435
52-53	0.0	0.0	0.0	0.7574958036536732	0.0017654531472015432
54-55	0.0	0.0	0.0	0.899837888719802	0.0019206578194829976
56-57	0.0	0.0	0.0	1.0675365371199135	0.0019400584035181795
58-59	0.0	0.0	0.0	1.2474187522941191	0.0019400584035181795
60-61	0.0	0.0	0.0	1.443888466818405	0.0019400584035181795
62-63	0.0	0.0	0.0	1.6503494821208098	0.00205646190772927
64-65	0.0	0.0	0.0	1.87289358158838	0.00205646190772927
66-67	0.0	0.0	0.0	2.1328032059077104	0.002095263075799634
68-69	0.0	0.0	0.0	2.4227255337294675	0.0021340642438699973
70-71	0.0	0.0	0.0	2.7386252435743326	0.002289268916151452
72-73	0.0	0.0	0.0	3.0721018825550725	0.002502675340538452
74-75	0.0	0.0	0.0	3.4136491644944478	0.0025996782607143605
76-77	0.0	0.0	0.0	3.7921545590208448	0.0025996782607143605
78-79	0.0	0.0	0.0	4.2250591911818915	0.0026190788447495424
80-81	0.0	0.0	0.0	4.686036468441846	0.002735482348960633
82-83	0.0	0.0	0.0	5.168703598653133	0.003104093445629087
84-85	0.0	0.0	0.0	5.666231576235371	0.003104093445629087
86-87	0.0	0.0	0.0	6.187447666924565	0.003142894613699451
88-89	0.0	0.0	0.0	6.7668849103033395	0.0031816957817698143
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2390	0.0	40.71595	1
GGTATCA	1485	0.0	35.1621	1
CAACGCA	2825	0.0	33.102806	5
TCAACGC	2835	0.0	32.819874	4
ATCAACG	3050	0.0	30.349518	3
TATCAAC	3295	0.0	29.100775	2
AACGCAG	3285	0.0	28.746931	6
GTACATG	6405	0.0	27.569725	1
TACATGG	6645	0.0	25.431013	2
ACATGGG	7180	0.0	23.073742	3
ACGCAGA	3965	0.0	22.982157	7
GTATACG	105	0.0022053514	22.604208	1
CGCAGAG	4440	0.0	20.311665	8
TATACCG	235	1.14087015E-7	20.199898	5
GAGTACA	4835	0.0	19.93004	1
CATGGGG	6340	0.0	19.917109	4
AGTACAT	4755	0.0	19.366888	2
ATGGGGG	3365	0.0	18.621124	5
GTCTTAG	870	0.0	18.00542	1
GTATAAG	835	0.0	17.054672	1
>>END_MODULE