FastQCFastQC Report
Mon 15 Aug 2016
SRR2921646_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921646_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2577242
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24350.040.0065421
GGTATCA14950.034.010951
TCAACGC30800.030.2082964
CAACGCA31750.029.3192185
ATCAACG33750.027.286563
GTACATG65250.026.4363961
TATCAAC36500.026.141112
AACGCAG36450.025.7972816
TACATGG68500.024.8092332
ACATGGG75700.021.697073
ACGCAGA46950.020.1415861
CATGGGG66000.019.3476924
CGCAGAG49000.018.9880712
AGAGTAC48300.017.798015
TACCGTA2152.400843E-517.670527
TATACCG2755.921029E-717.2705965
CAGAGTA54550.017.0561964
AGTACAT48200.015.7641597
TTTATAC7250.015.7142653
TATAGTG6350.015.7067225
Produced by FastQC (version 0.11.5)