##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921646_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2577242
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.902341728095383	31.0	31.0	34.0	26.0	34.0
2	30.068523250823944	31.0	31.0	34.0	26.0	34.0
3	30.13385588159746	31.0	31.0	34.0	26.0	34.0
4	33.59316820073551	37.0	35.0	37.0	28.0	37.0
5	33.21911834433864	37.0	35.0	37.0	27.0	37.0
6	33.16347785733742	37.0	35.0	37.0	26.0	37.0
7	32.98742221335831	37.0	35.0	37.0	26.0	37.0
8	32.907890683141126	37.0	35.0	37.0	25.0	37.0
9	34.35250899993093	39.0	35.0	39.0	25.0	39.0
10-11	34.17801161086153	39.0	35.0	39.0	25.0	39.0
12-13	34.05377822493968	39.0	35.0	39.0	23.0	39.0
14-15	35.12046365843797	40.0	36.0	41.0	23.0	41.0
16-17	35.05309687642837	40.0	36.0	41.0	22.0	41.0
18-19	34.97468320786329	40.0	36.0	41.0	20.0	41.0
20-21	34.89125914446528	40.0	35.5	41.0	18.5	41.0
22-23	34.774322124193226	39.5	35.0	41.0	18.0	41.0
24-25	34.6663446816403	39.0	35.0	41.0	18.0	41.0
26-27	34.509479901382946	39.0	35.0	41.0	17.0	41.0
28-29	34.362014704090655	39.0	34.5	41.0	16.0	41.0
30-31	34.19019866974075	39.0	34.0	41.0	15.5	41.0
32-33	34.14438690662344	39.0	34.0	41.0	15.0	41.0
34-35	34.29729920589529	39.0	35.0	41.0	15.0	41.0
36-37	34.29070126127077	39.0	35.0	41.0	14.0	41.0
38-39	34.179564821619394	39.0	35.0	41.0	9.0	41.0
40-41	34.08662535376965	39.0	34.0	41.0	9.0	41.0
42-43	33.94969389758509	39.0	34.0	41.0	8.5	41.0
44-45	33.81312659036288	39.0	34.0	41.0	7.5	41.0
46-47	33.64396649596739	39.0	34.0	41.0	3.5	41.0
48-49	33.481742692382014	39.0	33.0	40.5	2.0	41.0
50-51	33.30394972610256	38.5	33.0	40.0	2.0	41.0
52-53	33.11670130317603	38.0	33.0	40.0	2.0	41.0
54-55	32.888056496052755	38.0	33.0	40.0	2.0	41.0
56-57	32.63675180677639	38.0	33.0	40.0	2.0	41.0
58-59	32.37674265746096	37.0	32.0	40.0	2.0	41.0
60-61	32.082430365483724	37.0	32.0	40.0	2.0	41.0
62-63	31.767399607797792	36.0	32.0	39.5	2.0	41.0
64-65	31.42150911711046	36.0	31.0	39.0	2.0	41.0
66-67	31.06751189837819	35.0	31.0	39.0	2.0	40.0
68-69	30.70654773591304	35.0	31.0	38.0	2.0	40.0
70-71	30.329985697889448	35.0	30.5	37.0	2.0	39.5
72-73	29.98578422204822	35.0	30.0	37.0	2.0	39.0
74-75	29.59876837332311	35.0	30.0	36.0	2.0	39.0
76-77	27.11732910607541	32.0	26.0	34.0	2.0	36.0
78-79	28.585983194438086	34.0	29.0	35.0	2.0	37.0
80-81	28.78448259806413	34.0	29.0	35.0	2.0	37.0
82-83	28.63576315301396	34.0	29.0	35.0	2.0	36.0
84-85	28.44453004413245	34.0	29.0	35.0	2.0	36.0
86-87	28.24623531666797	34.0	29.0	35.0	2.0	35.5
88-89	27.998033944813876	34.0	29.0	35.0	2.0	35.0
90-91	27.791487178930034	34.0	29.0	35.0	2.0	35.0
92-93	27.61905013188517	34.0	29.0	35.0	2.0	35.0
94-95	27.487457134409574	34.0	28.5	35.0	2.0	35.0
96-97	27.2313267438603	34.0	27.0	35.0	2.0	35.0
98-99	26.983042337506525	34.0	27.0	35.0	2.0	35.0
100-101	25.842109316858874	32.5	23.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	132507.0
3	33503.0
4	16701.0
5	10984.0
6	9249.0
7	8906.0
8	8687.0
9	9285.0
10	9885.0
11	10202.0
12	10395.0
13	10753.0
14	11216.0
15	11685.0
16	11929.0
17	12021.0
18	11868.0
19	11090.0
20	10582.0
21	10940.0
22	11612.0
23	13088.0
24	15596.0
25	18805.0
26	22852.0
27	27180.0
28	32706.0
29	39675.0
30	47823.0
31	58261.0
32	74398.0
33	98307.0
34	139410.0
35	214033.0
36	372492.0
37	603293.0
38	402605.0
39	22718.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.51088881318986	22.056396466452235	13.559599195656826	28.87311552470108
2	14.192125759388244	23.214571873233332	32.91293512165539	29.680367245723037
3	16.402091970329348	25.826707001535066	27.361731750360484	30.409469277775102
4	13.027542159137647	18.22473749970898	31.954375780904414	36.79334456024896
5	14.728431876287443	36.950347949326535	33.588558265018946	14.732661909367078
6	35.71779174907234	35.154658816252905	15.384707912380504	13.742841522294253
7	30.363116851269687	30.663748301478865	20.04670108588949	18.926433761361952
8	27.80751671748326	35.014833686553295	18.43738383900309	18.740265756960348
9	26.83722632070497	14.774947317693613	17.752006894194217	40.6358194674072
10-11	26.68746607368432	25.062981409203815	26.699867312566145	21.549685204545725
12-13	26.91716793110439	21.996094648038156	26.73912811513901	24.34760930571844
14-15	24.0781493939177	23.50796892633829	23.436398075172868	28.977483604571148
16-17	24.86832823615322	26.753696393276222	24.13514136429563	24.242834006274926
18-19	24.859833862943624	26.11673595025938	25.253146862915106	23.7702833238819
20-21	25.43957026780272	25.180241755402015	25.054719538357613	24.32546843843766
22-23	25.090620128028334	25.0240955253717	24.99786593575613	24.88741841084384
24-25	24.974007941473587	25.32013544001417	24.94845724837466	24.757399370137584
26-27	24.958603096284687	25.431657907434293	24.940521355564044	24.669217640716976
28-29	25.069729649186527	25.541986018371066	24.816285462396433	24.571998870045974
30-31	25.178537229754156	25.432743587260926	24.70730178219981	24.681417400785108
32-33	24.96223296777666	25.38452911796469	24.729305548387398	24.923932365871256
34-35	25.151223475867006	25.389692570633205	24.893818099554007	24.56526585394578
36-37	25.198196861094864	25.350941778247282	24.88180360453171	24.569057756126142
38-39	25.21779095637895	25.388457894136447	24.815190036480857	24.578561113003747
40-41	25.13936435535801	25.2569750082213	24.919043244517436	24.684617391903256
42-43	25.07300989349054	25.297840785270587	24.82010424330474	24.809045077934133
44-45	25.01438153389706	25.267942770322875	24.857363316252695	24.860312379527368
46-47	25.1680444833333	25.32436087220548	24.800117959990608	24.707476684470606
48-49	25.01981954993421	25.353715423470923	24.855229004567715	24.771236022027153
50-51	25.011383617330413	25.342832647535463	24.83710320066383	24.80868053447029
52-53	25.21835764013109	25.29601962444426	24.775744472476653	24.709878262947996
54-55	24.86990036899152	25.18410288536662	24.999869041865665	24.94612770377619
56-57	24.937138107577653	25.291725745204452	24.89930456121235	24.87183158600555
58-59	24.97677476136407	25.155843873620555	24.91313333886962	24.95424802614576
60-61	24.958014627362356	25.288387513348397	24.951709060769364	24.80188879851988
62-63	24.95359990065091	25.284964039316627	24.92274735158032	24.838688708452143
64-65	24.8043226685282	25.060166982905997	24.979355655812835	25.15615469275297
66-67	24.815308477205512	25.317776562708357	24.836232516150623	25.030682443935515
68-69	24.9048931159909	25.151867270169927	24.879991910504717	25.06324770333445
70-71	24.774089881101776	24.98421400088553	25.019367329377758	25.222328788634936
72-73	24.762271620271097	25.16617990937582	24.856485838758818	25.215062631594265
74-75	24.704863260458097	25.20579721169083	24.80865933658221	25.280680191268868
76-77	24.730562359732676	24.972791928997818	25.04467953945287	25.251966171816637
78-79	24.57624420695286	25.050151084144723	25.093731182424158	25.27987352647826
80-81	24.73169603974445	25.118732351743972	24.833021560013144	25.31655004849843
82-83	24.577689214423252	25.210129365757144	24.887874407693758	25.324307012125846
84-85	24.571733675339548	25.087770748181942	24.99781167519321	25.3426839012853
86-87	24.504259987448414	25.19873179695163	24.877041646373385	25.419966569226567
88-89	24.461134301792796	24.9803769492749	24.971644788942324	25.58684395998998
90-91	24.290300151218254	25.126697558627463	25.00883484146395	25.574167448690332
92-93	24.36454604470899	25.089744995541135	24.974095878726597	25.571613081023276
94-95	24.20782290824096	24.88976292553587	25.169830188243616	25.732583977979555
96-97	24.099333545337405	25.026595904647834	25.05841591823343	25.815654631781328
98-99	24.114038760234344	24.886864923528332	25.013357369484822	25.9857389467525
100-101	24.040135959398427	24.84048827427791	25.079794664059225	26.039581102264435
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10.0
1	10.0
2	19.0
3	28.5
4	27.5
5	28.5
6	45.5
7	98.0
8	146.0
9	157.5
10	152.0
11	140.0
12	148.5
13	137.0
14	139.5
15	178.5
16	200.5
17	220.0
18	256.5
19	303.0
20	332.0
21	377.0
22	467.0
23	572.5
24	688.5
25	817.5
26	1039.5
27	1350.0
28	1700.0
29	2163.0
30	2839.5
31	4180.0
32	6637.5
33	9784.0
34	13768.5
35	19536.0
36	27640.0
37	36091.0
38	44274.5
39	51695.0
40	58287.5
41	65551.5
42	73377.0
43	82445.0
44	91466.5
45	100652.5
46	107798.0
47	113200.0
48	119227.0
49	122167.5
50	122367.0
51	122966.0
52	123560.0
53	123255.5
54	121407.5
55	116043.5
56	108006.5
57	98573.5
58	89206.0
59	79335.5
60	67207.0
61	55581.5
62	45361.0
63	35080.5
64	25922.5
65	19321.0
66	14823.5
67	11869.0
68	9175.0
69	6788.0
70	5157.5
71	3768.0
72	2781.0
73	2084.0
74	1539.0
75	1167.0
76	883.0
77	595.5
78	353.0
79	192.5
80	118.5
81	77.0
82	40.5
83	19.5
84	16.5
85	9.5
86	4.5
87	4.0
88	5.5
89	4.0
90	1.0
91	0.5
92	0.5
93	1.5
94	1.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1055003759833186
2	1.1640350421109078E-4
3	0.0058201752105545386
4	0.003957719143177087
5	0.016684502270256342
6	0.0020564619077292705
7	0.0
8	0.0
9	3.8801168070363586E-5
10-11	0.03439723549437732
12-13	3.686110966684541E-4
14-15	0.002929488189312451
16-17	0.0
18-19	0.0012610379622868166
20-21	3.4921051263327234E-4
22-23	0.0
24-25	4.850146008795449E-4
26-27	0.002192265995975543
28-29	0.005664970538273084
30-31	0.015462265476039891
32-33	0.01004950253022417
34-35	0.006926008500559901
36-37	0.0026966811808902696
38-39	0.0
40-41	0.0036667103826493594
42-43	0.007527426605650537
44-45	0.005917178130730448
46-47	0.0037637133028252685
48-49	0.009351081504957625
50-51	0.002851885853171724
52-53	0.0018624560673774525
54-55	0.003201096365804996
56-57	0.0063633915635396285
58-59	0.01179555509339053
60-61	0.005975379882835993
62-63	0.018217148409035707
64-65	0.03377641680525151
66-67	0.048792468848482214
68-69	0.04118743990669095
70-71	0.053681416025348025
72-73	0.025880379102932515
74-75	0.021534648279051792
76-77	0.17367402828294742
78-79	0.05917178130730447
80-81	0.053759018361488754
82-83	0.021554048863086976
84-85	0.04153665041932422
86-87	0.05709591881554002
88-89	0.24390414249030554
90-91	0.2504421393101618
92-93	0.035483668200347505
94-95	0.007081213172841355
96-97	0.009603289097414988
98-99	5.044151849147267E-4
100-101	7.760233614072717E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2577242.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.417174634702555
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.21896220357743	50.07040972875296
2	10.977877396070856	13.704161810978166
3	3.543735443370159	6.635698620840609
4	1.6961616140998363	4.2347846270379375
5	0.9088151162116089	2.8362835909618744
6	0.5686339494377525	2.129551471516609
7	0.4036573814801001	1.7636607290701112
8	0.2787372015860584	1.3918390870868236
9	0.21769439818166833	1.2229082341471498
>10	1.1212621534031801	12.229667521170787
>50	0.04917932952176644	2.0487849398949876
>100	0.014722055125431323	1.5100649212309323
>500	4.99340385941543E-4	0.1806541354014053
>1k	6.241754823427289E-5	0.04153058190963547
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.880116807036359E-5	0.0
2	0.0	0.0	0.0	3.880116807036359E-5	0.0
3	0.0	0.0	0.0	3.880116807036359E-5	0.0
4	0.0	0.0	0.0	3.880116807036359E-5	0.0
5	0.0	0.0	0.0	3.880116807036359E-5	0.0
6	0.0	0.0	0.0	3.880116807036359E-5	3.880116807036359E-5
7	0.0	0.0	0.0	3.880116807036359E-5	3.880116807036359E-5
8	0.0	0.0	0.0	3.880116807036359E-5	3.880116807036359E-5
9	0.0	0.0	0.0	3.880116807036359E-5	3.880116807036359E-5
10-11	0.0	0.0	0.0	3.880116807036359E-5	3.880116807036359E-5
12-13	0.0	0.0	0.0	5.8201752105545385E-5	1.5520467228145434E-4
14-15	0.0	0.0	0.0	7.760233614072718E-5	2.1340642438699974E-4
16-17	0.0	0.0	0.0	7.760233614072718E-5	3.1040934456290874E-4
18-19	0.0	0.0	0.0	1.3580408824627257E-4	3.4921051263327234E-4
20-21	0.0	0.0	0.0	2.1340642438699974E-4	4.656140168443631E-4
22-23	0.0	0.0	0.0	2.3280700842218154E-4	5.626169370202721E-4
24-25	0.0	0.0	0.0	3.4921051263327234E-4	6.402192731609993E-4
26-27	0.0	0.0	0.0	4.462134328091813E-4	6.984210252665447E-4
28-29	0.0	0.0	0.0	9.700292017590898E-4	6.984210252665447E-4
30-31	0.0	0.0	0.0	0.003880116807036359	6.984210252665447E-4
32-33	0.0	0.0	0.0	0.012474575534621894	6.984210252665447E-4
34-35	0.0	0.0	0.0	0.026501197792058333	6.984210252665447E-4
36-37	0.0	0.0	0.0	0.051469749445337304	6.984210252665447E-4
38-39	1.9400584035181796E-5	0.0	0.0	0.09659550791117016	8.53625697547999E-4
40-41	3.880116807036359E-5	0.0	0.0	0.14969490641546274	9.312280336887262E-4
42-43	3.880116807036359E-5	0.0	0.0	0.2087890853866265	9.312280336887262E-4
44-45	5.8201752105545385E-5	0.0	0.0	0.2823172988799655	9.50628617723908E-4
46-47	7.760233614072718E-5	0.0	0.0	0.3716375877779424	9.700292017590898E-4
48-49	7.760233614072718E-5	0.0	0.0	0.46823309568911264	0.001047631537899817
50-51	7.760233614072718E-5	0.0	0.0	0.5749557084666477	0.001047631537899817
52-53	9.700292017590897E-5	1.9400584035181796E-5	0.0	0.6873626923664911	0.001144634458075726
54-55	1.1640350421109077E-4	3.880116807036359E-5	0.0	0.8175406112425608	0.001241637378251635
56-57	1.1640350421109077E-4	3.880116807036359E-5	0.0	0.9704948157759341	0.001241637378251635
58-59	1.5520467228145437E-4	7.760233614072718E-5	0.0	1.1349147654740999	0.001241637378251635
60-61	1.5520467228145437E-4	7.760233614072718E-5	0.0	1.3147775800642703	0.001241637378251635
62-63	1.5520467228145437E-4	7.760233614072718E-5	0.0	1.5052719147057203	0.0013968420505330894
64-65	1.5520467228145437E-4	7.760233614072718E-5	0.0	1.711112111318999	0.0013968420505330894
66-67	1.5520467228145437E-4	7.760233614072718E-5	0.0	1.950457116561037	0.001416242634568271
68-69	1.5520467228145437E-4	7.760233614072718E-5	0.0	2.21752555638935	0.0014356432186034527
70-71	1.5520467228145437E-4	7.760233614072718E-5	0.0	2.5099117583835744	0.0014356432186034527
72-73	1.5520467228145437E-4	7.760233614072718E-5	0.0	2.820825518131398	0.0015714473068497254
74-75	1.5520467228145437E-4	7.760233614072718E-5	0.0	3.138432479371359	0.0016102484749200888
76-77	1.5520467228145437E-4	7.760233614072718E-5	0.0	3.4912709012192105	0.0016490496429904526
78-79	1.5520467228145437E-4	7.760233614072718E-5	0.0	3.8970535168990725	0.0016684502270256343
80-81	1.5520467228145437E-4	7.760233614072718E-5	0.0	4.328444903505375	0.001687850811060816
82-83	1.5520467228145437E-4	7.760233614072718E-5	0.0	4.780769520285638	0.001901257235447816
84-85	1.5520467228145437E-4	7.760233614072718E-5	0.0	5.248013186188957	0.001901257235447816
86-87	1.5520467228145437E-4	7.760233614072718E-5	0.0	5.7364228892746585	0.0019788595715885433
88-89	1.5520467228145437E-4	7.760233614072718E-5	0.0	6.281521098911162	0.0020176607396589067
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2435	0.0	40.006542	1
GGTATCA	1495	0.0	34.01095	1
TCAACGC	3080	0.0	30.208296	4
CAACGCA	3175	0.0	29.319218	5
ATCAACG	3375	0.0	27.28656	3
GTACATG	6525	0.0	26.436396	1
TATCAAC	3650	0.0	26.14111	2
AACGCAG	3645	0.0	25.797281	6
TACATGG	6850	0.0	24.809233	2
ACATGGG	7570	0.0	21.69707	3
ACGCAGA	4695	0.0	20.141586	1
CATGGGG	6600	0.0	19.347692	4
CGCAGAG	4900	0.0	18.988071	2
AGAGTAC	4830	0.0	17.79801	5
TACCGTA	215	2.400843E-5	17.67052	7
TATACCG	275	5.921029E-7	17.270596	5
CAGAGTA	5455	0.0	17.056196	4
AGTACAT	4820	0.0	15.764159	7
TTTATAC	725	0.0	15.714265	3
TATAGTG	635	0.0	15.706722	5
>>END_MODULE