FastQCFastQC Report
Mon 15 Aug 2016
SRR2921645_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921645_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1330651
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25800.043.37111
GGTATCA17500.037.7526321
GTACATG74050.037.616911
TACATGG77050.035.5289342
ACATGGG77200.034.3806883
TCAACGC30700.033.729344
CATGGGG66400.033.698984
ATGGGGG42750.033.5476075
CAACGCA31250.032.944035
TATCAAC33650.032.1872062
ATCAACG32950.031.4261253
AACGCAG33150.030.69456
ACGCAGA41300.024.6308677
TGGGGGG34650.024.213856
GAGTACA51650.024.1997721
AGTACAT51250.023.5721452
CGCAGAG43700.023.2772678
GTATAGG4500.022.4853021
AGAGTAC44550.021.2388045
GGGTTAG5400.020.9421941
Produced by FastQC (version 0.11.5)