##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921645_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1330651
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.70346469510037	31.0	31.0	34.0	26.0	34.0
2	30.05252917556895	31.0	31.0	34.0	26.0	34.0
3	30.13468369993334	31.0	31.0	34.0	26.0	34.0
4	33.58173931406507	37.0	35.0	37.0	28.0	37.0
5	33.616018024260306	37.0	35.0	37.0	28.0	37.0
6	33.59530410302927	37.0	35.0	37.0	28.0	37.0
7	33.532337930832355	37.0	35.0	37.0	28.0	37.0
8	33.459321790612265	37.0	35.0	37.0	28.0	37.0
9	35.174273344400596	39.0	37.0	39.0	28.0	39.0
10-11	35.18834916142549	39.0	37.0	39.0	28.0	39.0
12-13	35.11902369592026	39.0	37.0	39.0	27.0	39.0
14-15	36.40616435113339	40.0	38.0	41.0	27.0	41.0
16-17	36.37930005688945	40.0	38.0	41.0	27.0	41.0
18-19	36.335754454022876	40.0	38.0	41.0	27.0	41.0
20-21	36.398123925807745	40.0	38.0	41.0	27.0	41.0
22-23	36.33940492285355	40.0	38.0	41.0	27.0	41.0
24-25	36.3371346055427	40.0	38.0	41.0	27.0	41.0
26-27	36.30885333569809	40.0	37.5	41.0	27.5	41.0
28-29	36.25392420702348	40.0	37.0	41.0	27.0	41.0
30-31	36.150576296865225	40.0	37.0	41.0	27.0	41.0
32-33	36.05621120789749	40.0	37.0	41.0	27.0	41.0
34-35	35.92840835049911	40.0	37.0	41.0	26.5	41.0
36-37	35.83386891078126	40.0	37.0	41.0	26.0	41.0
38-39	35.714459689279906	40.0	36.0	41.0	25.0	41.0
40-41	35.598909481148695	40.0	36.0	41.0	25.0	41.0
42-43	35.3659197640854	40.0	35.5	41.0	24.0	41.0
44-45	35.05013034973107	40.0	35.0	41.0	23.5	41.0
46-47	34.81129424619979	39.5	35.0	41.0	23.0	41.0
48-49	34.61762926567522	39.0	35.0	41.0	22.0	41.0
50-51	34.41932144491681	39.0	34.5	41.0	21.0	41.0
52-53	34.221847050804456	39.0	34.0	40.5	20.0	41.0
54-55	33.9828955902036	38.0	34.0	40.0	18.5	41.0
56-57	33.73279883305239	38.0	34.0	40.0	18.0	41.0
58-59	33.743659682366	38.0	34.0	40.0	18.0	41.0
60-61	33.52859502604365	37.5	34.0	40.0	16.5	41.0
62-63	33.20786028793425	37.0	33.0	40.0	13.0	41.0
64-65	32.90848013491141	36.5	33.0	39.5	12.0	41.0
66-67	32.58572570869447	36.0	33.0	39.0	8.0	41.0
68-69	32.2514599996543	35.0	33.0	39.0	7.5	40.5
70-71	31.883759903986846	35.0	32.5	38.0	6.5	40.0
72-73	31.532641541621356	35.0	32.0	37.0	2.0	39.5
74-75	31.170537203218576	35.0	32.0	37.0	2.0	39.0
76-77	30.869217773856555	35.0	32.0	36.0	2.0	39.0
78-79	30.594841171727225	35.0	31.5	36.0	2.0	37.5
80-81	30.346059560320477	35.0	31.0	35.0	2.0	37.0
82-83	30.12790168120717	35.0	31.0	35.0	2.0	36.5
84-85	29.92835161135414	35.0	31.0	35.0	2.0	36.0
86-87	29.7615479190261	35.0	31.0	35.0	2.0	36.0
88-89	29.61940583969801	35.0	31.0	35.0	2.0	35.5
90-91	29.447903319503013	34.0	31.0	35.0	2.0	35.0
92-93	29.37646046934921	34.0	31.0	35.0	2.0	35.0
94-95	29.26672921750331	34.0	30.0	35.0	2.0	35.0
96-97	29.195228500936757	34.0	30.0	35.0	2.0	35.0
98-99	29.11927019180837	34.0	30.0	35.0	2.0	35.0
100-101	29.02342800629166	34.0	30.0	35.0	2.0	35.0
102-103	28.176863430005312	33.5	28.0	34.5	2.0	35.0
104-105	29.08168219916417	34.0	30.5	35.0	2.0	35.0
106-107	29.355147968926488	35.0	31.0	35.0	2.0	35.0
108-109	29.37244551726937	35.0	31.0	35.0	2.0	35.0
110-111	29.32900212001494	35.0	31.0	35.0	2.0	35.0
112-113	29.26359729185188	35.0	31.0	35.0	2.0	35.0
114-115	29.16256591698349	35.0	31.0	35.0	2.0	35.0
116-117	29.06532441639468	35.0	31.0	35.0	2.0	35.0
118-119	28.807155670419966	35.0	30.5	35.0	2.0	35.0
120-121	28.69965302697702	34.0	30.0	35.0	2.0	35.0
122-123	28.487096917223223	34.0	30.0	35.0	2.0	35.0
124-125	27.74667174187672	34.0	28.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	58222.0
3	9860.0
4	4914.0
5	4154.0
6	4382.0
7	4813.0
8	4870.0
9	5137.0
10	5556.0
11	5860.0
12	5987.0
13	6190.0
14	6223.0
15	5406.0
16	4505.0
17	4190.0
18	3734.0
19	3604.0
20	3489.0
21	3662.0
22	4134.0
23	5623.0
24	8566.0
25	11111.0
26	11702.0
27	12244.0
28	14838.0
29	17996.0
30	21456.0
31	27120.0
32	34964.0
33	48541.0
34	76671.0
35	126141.0
36	253585.0
37	396505.0
38	104626.0
39	70.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.451101397747216	17.360103130367456	12.199976697949044	24.98881877393628
2	16.644319211846355	19.119851003606158	38.80866018991328	25.427169594634208
3	20.238148028172507	23.76985788231191	27.72200580676534	28.26998828275024
4	13.790851245172862	14.815129372610858	34.83537743010512	36.55864195211117
5	14.648245776228421	37.38340376084411	32.08029487855952	15.88805558436795
6	34.01498859269255	33.38974929498853	17.482102976430916	15.113159135887999
7	29.846273924767164	32.43414156574414	19.22816637065399	18.49141813883471
8	27.626952978150705	34.84413768214012	18.5447587254327	18.984150614276476
9	28.08778372039705	16.40250617334992	18.11488256540744	37.39482754084559
10-11	26.82253710262494	25.597267956572722	25.997269911469488	21.58292502933285
12-13	26.317858370929343	22.84359245612024	26.408351857314923	24.430197315635493
14-15	24.152690485372087	24.131717756684775	23.64953292455305	28.06605883339009
16-17	24.824717064606133	27.03858385897227	24.19545386374221	23.941245212679384
18-19	24.63247234802692	26.436466622158868	25.616756283719166	23.31430474609504
20-21	25.344322174975765	25.448474864095967	25.27918445926111	23.928018501667157
22-23	25.29553653286001	24.950429100739722	25.201337728275163	24.552696638125106
24-25	24.775406816146127	25.14767088758896	25.2326180605959	24.844304235669018
26-27	24.470968142115225	25.398980111612556	25.366800809745516	24.763250936526703
28-29	24.721321163051133	26.064144720148292	25.03713149039844	24.177402626402134
30-31	25.411999679774006	25.430792284959036	25.20253730238166	23.95467073288529
32-33	24.828257580740644	25.365256857199235	25.321581266855226	24.484904295204892
34-35	24.822396966181557	25.833657250391518	25.218691004177966	24.12525477924896
36-37	24.937515102161072	25.724800388419126	25.17028097629388	24.16740353312592
38-39	25.075824599567188	25.75345410647991	25.10183234326012	24.068888950692777
40-41	24.892255678355994	25.399015800495672	25.394429736041918	24.314298785106416
42-43	24.827227476431226	25.499901530560855	25.03983878072284	24.633032212285077
44-45	24.405854007586967	25.582195049904584	25.532061221897433	24.479889720611016
46-47	24.36242438091573	25.373773257002657	25.451008454905168	24.81279390717645
48-49	24.43657220607056	25.63719087630542	25.277414965730706	24.648821951893314
50-51	24.48615237740856	25.713109809382594	25.454104673255497	24.346633139953354
52-53	24.67081154341409	25.65719298482993	25.22218388549496	24.44981158626102
54-55	24.426769977359445	25.219978712464936	25.54436556831405	24.808885741861577
56-57	24.487850713171593	25.618589442429478	25.123747956326465	24.769811888072464
58-59	24.538914229684696	25.683966598505208	25.392629918608378	24.384489253201718
60-61	24.97221575979984	25.64850878402935	25.351742655357786	24.027532800813027
62-63	24.8280893183858	25.645059691417693	25.429851872676352	24.096999117520156
64-65	24.556313871315332	25.249359102288437	25.433369376195397	24.76095765020083
66-67	24.605382049111686	25.489222521074606	25.269493352497307	24.635902077316402
68-69	24.391330166270784	25.467166781923687	25.54098138849635	24.60052166330918
70-71	24.396318325593004	25.400380588054404	25.604771861445496	24.59852922490709
72-73	24.479297817820196	25.835389783420666	25.188162364219796	24.49715003453934
74-75	24.462949984310793	25.68340119463448	25.23358129170088	24.620067529353847
76-77	24.458445765654712	25.233157700893692	25.408213885736192	24.9001826477154
78-79	24.32946541025881	25.307214422606023	25.675213983607463	24.688106183527704
80-81	24.525399529542995	25.586915597728066	25.266412534195325	24.621272338533615
82-83	24.699727775232468	25.61276881866929	25.28371623828443	24.40378716781381
84-85	24.72955635442041	25.85405926648573	24.91760610116956	24.498778277924295
86-87	24.239394898306628	25.947575992862408	25.283500485936404	24.52952862289456
88-89	24.18746647995036	25.500982628392833	25.432918370622097	24.878632521034714
90-91	24.673983879781627	25.331277778738237	25.358375320115545	24.636363021364588
92-93	24.60721848418972	25.742920650787177	25.272135238070188	24.377725626952916
94-95	24.466122962518686	25.490810618239866	25.3093378970209	24.733728522220545
96-97	24.28056938865169	25.422030005761233	25.435897551532587	24.86150305405449
98-99	24.164062179997618	25.51203199563491	25.26176077453306	25.06214504983441
100-101	24.159434439456355	25.58833012777874	25.41020200630889	24.84203342645601
102-103	23.937400866605767	25.553709679177	25.57073215824367	24.938157295973564
104-105	24.32292381025746	25.535503920246054	25.355508918019602	24.78606335147688
106-107	24.151488176540813	25.487876684764494	25.562126753434534	24.79850838526016
108-109	24.170130056081916	25.457575155143186	25.517704531011535	24.85459025776336
110-111	24.0489239180468	25.815257724211136	25.514086293224114	24.62173206451795
112-113	23.92134788395476	25.93657538333663	25.489678112216946	24.652398620491667
114-115	24.02260250497568	26.021592337155536	25.395836966129316	24.559968191739472
116-117	23.895449750878473	25.752540911808463	25.44571878859937	24.9062905487137
118-119	23.97335527728733	25.576236024105192	25.60328918167589	24.847119516931585
120-121	23.881585770392157	25.746598989654558	25.756857257517847	24.61495798243544
122-123	23.818772853203292	25.811987394031217	25.500821221675746	24.868418531089752
124-125	23.769007317037318	25.728697840243363	25.5177012147571	24.98459362796222
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	391.0
1	319.5
2	226.5
3	183.5
4	156.0
5	168.5
6	178.5
7	179.0
8	197.5
9	224.0
10	249.5
11	241.0
12	250.5
13	286.0
14	312.0
15	342.5
16	394.5
17	453.0
18	482.0
19	527.5
20	588.0
21	678.0
22	780.0
23	957.5
24	1204.5
25	1499.0
26	1956.5
27	2529.0
28	3299.5
29	4252.0
30	5536.0
31	7144.5
32	9152.5
33	11398.0
34	13680.0
35	15890.5
36	18550.0
37	21433.5
38	23870.5
39	26296.0
40	28834.0
41	31075.0
42	33123.5
43	36587.0
44	40955.0
45	44752.5
46	47827.0
47	51067.0
48	55443.0
49	59223.0
50	61922.0
51	63366.0
52	62307.0
53	60986.5
54	60376.5
55	57974.0
56	53891.5
57	49563.5
58	45427.5
59	40739.0
60	34686.5
61	29279.0
62	24436.0
63	18734.0
64	13958.0
65	10781.0
66	8268.5
67	6451.0
68	5033.5
69	3895.0
70	3123.0
71	2386.0
72	1807.0
73	1494.0
74	1211.0
75	936.5
76	679.0
77	475.0
78	342.0
79	234.5
80	154.5
81	106.5
82	78.0
83	66.5
84	51.5
85	37.5
86	34.0
87	28.5
88	28.0
89	27.0
90	20.5
91	19.0
92	15.0
93	11.5
94	13.0
95	12.0
96	9.0
97	7.0
98	5.0
99	6.5
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.022244750877577966
2	0.011197526624186207
3	0.010070258843227865
4	0.011498131365775098
5	0.015405993006430686
6	0.09318746989255636
7	0.05658884260410881
8	0.04742039798564763
9	0.025250798293466884
10-11	0.04941190439867404
12-13	0.01266297473943205
14-15	0.02645321725982245
16-17	0.03340470190906556
18-19	0.0411076984122809
20-21	0.02949684026840997
22-23	0.0364859005103517
24-25	0.03099986397635443
26-27	0.045353740387224
28-29	0.04362526312308787
30-31	0.025588978627754385
32-33	0.029196235526821084
34-35	0.04343738515959482
36-37	0.009732078508940362
38-39	0.020892029540427955
40-41	0.04016830859481562
42-43	0.02171869257979741
44-45	0.015932051304211246
46-47	0.02247020443376964
48-49	0.028444723672848855
50-51	0.027693211818876625
52-53	0.025100495922672435
54-55	0.022169599692180745
56-57	0.024499286439494655
58-59	0.017397499419457094
60-61	0.0233344430658377
62-63	0.023221716287741865
64-65	0.02183141935789324
66-67	0.02833199689475302
68-69	0.021868994950591854
70-71	0.04539131597992261
72-73	0.021342936652811294
74-75	0.008905415469570908
76-77	0.01660841197278625
78-79	0.026340490481726615
80-81	0.018224162458826545
82-83	0.02378535017822104
84-85	0.011422980180377875
86-87	0.01728477264136126
88-89	0.02183141935789324
90-91	0.020441122428044616
92-93	0.00815390361559868
94-95	0.011122375438788983
96-97	0.015518719784526522
98-99	0.00710178702003756
100-101	0.011122375438788983
102-103	0.004321193160340315
104-105	0.0043963443457375375
106-107	9.393898174652859E-4
108-109	0.014015696076582065
110-111	0.0010145410028625087
112-113	0.0024048379327111314
114-115	0.013452062186102893
116-117	0.005673914497490326
118-119	0.2730994077335079
120-121	0.0012775701517527885
122-123	8.64238632068063E-4
124-125	0.30733077268194287
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1330651.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.33036221451023
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.06418876663842	54.266135391789405
2	9.437319071043039	12.33086946490294
3	2.9022451656812542	5.688141837278029
4	1.397415173315204	3.6517455774693945
5	0.8052522744720552	2.6303711382658794
6	0.5073771041915027	1.9888277997708104
7	0.3497281009591142	1.5993504458576213
8	0.2692407556052669	1.4071676869280412
9	0.19614543599826653	1.153282714244081
>10	1.0112545423028245	11.550579713641747
>50	0.044345498251362574	1.9484241175137844
>100	0.015143938239890242	1.6309321922032283
>500	3.4417330186967967E-4	0.1541719201351192
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5030237079444574E-4
2	0.0	0.0	0.0	0.0	1.5030237079444574E-4
3	0.0	0.0	0.0	0.0	1.5030237079444574E-4
4	0.0	0.0	0.0	0.0	1.5030237079444574E-4
5	0.0	0.0	0.0	0.0	1.5030237079444574E-4
6	0.0	0.0	0.0	0.0	1.5030237079444574E-4
7	0.0	0.0	0.0	0.0	1.5030237079444574E-4
8	0.0	0.0	0.0	0.0	1.5030237079444574E-4
9	0.0	0.0	0.0	0.0	1.5030237079444574E-4
10-11	0.0	0.0	0.0	0.0	1.5030237079444574E-4
12-13	0.0	0.0	0.0	7.515118539722287E-5	3.381803342875029E-4
14-15	0.0	3.7575592698611434E-5	0.0	7.515118539722287E-5	4.509071123833372E-4
16-17	0.0	7.515118539722287E-5	0.0	7.515118539722287E-5	4.509071123833372E-4
18-19	0.0	7.515118539722287E-5	0.0	7.515118539722287E-5	5.2605829778056E-4
20-21	0.0	7.515118539722287E-5	0.0	7.515118539722287E-5	6.01209483177783E-4
22-23	0.0	7.515118539722287E-5	0.0	7.515118539722287E-5	6.763606685750058E-4
24-25	0.0	7.515118539722287E-5	0.0	2.6302914889028E-4	6.763606685750058E-4
26-27	0.0	7.515118539722287E-5	0.0	4.884827050819486E-4	6.763606685750058E-4
28-29	0.0	7.515118539722287E-5	0.0	0.0016909016714375144	6.763606685750058E-4
30-31	0.0	7.515118539722287E-5	0.0	0.00627512398066811	6.763606685750058E-4
32-33	0.0	7.515118539722287E-5	0.0	0.01777325534644321	8.266630393694515E-4
34-35	0.0	7.515118539722287E-5	0.0	0.03212713175731277	8.266630393694515E-4
36-37	0.0	7.515118539722287E-5	0.0	0.05395855111520602	8.266630393694515E-4
38-39	0.0	7.515118539722287E-5	0.0	0.09330019667065219	8.266630393694515E-4
40-41	0.0	7.515118539722287E-5	0.0	0.1463193579683929	8.266630393694515E-4
42-43	0.0	7.515118539722287E-5	0.0	0.18990704549878218	8.266630393694515E-4
44-45	0.0	7.515118539722287E-5	0.0	0.23480987877362286	8.266630393694515E-4
46-47	0.0	7.515118539722287E-5	0.0	0.2870775282173913	8.266630393694515E-4
48-49	0.0	7.515118539722287E-5	0.0	0.34291485896752794	8.266630393694515E-4
50-51	0.0	7.515118539722287E-5	0.0	0.4085969950047007	9.018142247666744E-4
52-53	0.0	7.515118539722287E-5	0.0	0.4809675865422263	0.00105211659556112
54-55	0.0	7.515118539722287E-5	0.0	0.5564569522737367	0.0011272677809583429
56-57	0.0	7.515118539722287E-5	0.0	0.6338626732328763	0.0011272677809583429
58-59	0.0	7.515118539722287E-5	0.0	0.7296428590216368	0.0011272677809583429
60-61	0.0	7.515118539722287E-5	0.0	0.8276400047796153	0.0011272677809583429
62-63	0.0	7.515118539722287E-5	0.0	0.9497982566428012	0.001202418966355566
64-65	0.0	7.515118539722287E-5	0.0	1.0556111256820908	0.001202418966355566
66-67	0.0	7.515118539722287E-5	0.0	1.1667973044772824	0.001202418966355566
68-69	0.0	7.515118539722287E-5	0.0	1.2954937094700263	0.001202418966355566
70-71	0.0	7.515118539722287E-5	0.0	1.4371536939437912	0.001202418966355566
72-73	0.0	7.515118539722287E-5	0.0	1.567653727386069	0.0012399945590541774
74-75	0.0	7.515118539722287E-5	0.0	1.7004458719829616	0.0012775701517527887
76-77	0.0	7.515118539722287E-5	0.0	1.8657409042641535	0.001390296929848623
78-79	0.0	7.515118539722287E-5	0.0	2.0527170535324437	0.0015030237079444572
80-81	0.0	7.515118539722287E-5	0.0	2.2288338565108354	0.00157817489334168
82-83	0.0	7.515118539722287E-5	0.0	2.4093845794276634	0.001728477264136126
84-85	0.0	7.515118539722287E-5	0.0	2.606506138724579	0.001728477264136126
86-87	0.0	7.515118539722287E-5	0.0	2.8382723944896147	0.0018036284495333487
88-89	0.0	7.515118539722287E-5	0.0	3.1010009386383057	0.0018787796349305716
90-91	0.0	7.515118539722287E-5	0.0	3.3688773389867066	0.0018787796349305716
92-93	0.0	7.515118539722287E-5	0.0	3.6172144311318295	0.002179384376519463
94-95	0.0	7.515118539722287E-5	0.0	3.8764860207522482	0.0022545355619166857
96-97	0.0	7.515118539722287E-5	0.0	4.177767123009715	0.0022921111546152974
98-99	0.0	7.515118539722287E-5	0.0	4.5169244227073815	0.0023296867473139088
100-101	0.0	7.515118539722287E-5	0.0	4.874568914012765	0.0023296867473139088
102-103	0.0	7.515118539722287E-5	0.0	5.277153814185688	0.00236726234001252
104-105	0.0	7.515118539722287E-5	0.0	5.676169033052243	0.002404837932711132
106-107	0.0	7.515118539722287E-5	0.0	6.095249618419857	0.002404837932711132
108-109	0.0	7.515118539722287E-5	0.0	6.524738642964985	0.002442413525409743
110-111	0.0	7.515118539722287E-5	0.0	7.031107330171473	0.0024799891181083544
112-113	0.0	7.515118539722287E-5	0.0	7.507453118811769	0.0025551403035055775
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2580	0.0	43.3711	1
GGTATCA	1750	0.0	37.752632	1
GTACATG	7405	0.0	37.61691	1
TACATGG	7705	0.0	35.528934	2
ACATGGG	7720	0.0	34.380688	3
TCAACGC	3070	0.0	33.72934	4
CATGGGG	6640	0.0	33.69898	4
ATGGGGG	4275	0.0	33.547607	5
CAACGCA	3125	0.0	32.94403	5
TATCAAC	3365	0.0	32.187206	2
ATCAACG	3295	0.0	31.426125	3
AACGCAG	3315	0.0	30.6945	6
ACGCAGA	4130	0.0	24.630867	7
TGGGGGG	3465	0.0	24.21385	6
GAGTACA	5165	0.0	24.199772	1
AGTACAT	5125	0.0	23.572145	2
CGCAGAG	4370	0.0	23.277267	8
GTATAGG	450	0.0	22.485302	1
AGAGTAC	4455	0.0	21.238804	5
GGGTTAG	540	0.0	20.942194	1
>>END_MODULE