FastQCFastQC Report
Mon 15 Aug 2016
SRR2921645_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921645_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1330651
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25340.19043310379656272No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21260.1597714201544958No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14220.10686498563485092No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24500.046.7223851
GTATCAA38650.045.196831
GTACATG74700.034.2394371
TATCAAC50850.034.0001262
TCAACGC50800.033.793794
CAACGCA51850.033.3167885
ATCAACG51550.033.3071373
TACATGG77650.032.93742
ACATGGG77450.032.4836273
AACGCAG54050.031.8600716
CATGGGG64600.031.6497064
ATGGGGG40350.031.2972475
TGGGGGG30600.027.0668666
ACGCAGA63600.026.9803317
CGCAGAG66550.025.7833888
TAACGCA1250.001616429523.8433934
GCAGAGT73800.023.3311989
TATGTAA6250.022.8931082
AGAGTAC68250.022.04216810-11
GAGTACT33800.022.0151312-13
Produced by FastQC (version 0.11.5)