##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921645_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1330651 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.755019911306572 33.0 31.0 34.0 30.0 34.0 2 31.925208037268977 33.0 31.0 34.0 30.0 34.0 3 31.97999625747097 34.0 31.0 34.0 30.0 34.0 4 35.368690212535064 37.0 35.0 37.0 33.0 37.0 5 35.22748188668554 37.0 35.0 37.0 33.0 37.0 6 35.25484819084794 37.0 35.0 37.0 33.0 37.0 7 35.143020972441306 37.0 35.0 37.0 33.0 37.0 8 35.18145178562974 37.0 35.0 37.0 33.0 37.0 9 36.90195776353078 39.0 38.0 39.0 34.0 39.0 10-11 36.84774895896821 39.0 38.0 39.0 33.5 39.0 12-13 36.77167942608543 39.0 38.0 39.0 33.0 39.0 14-15 38.184200815991574 41.0 39.0 41.0 33.5 41.0 16-17 38.19632984155875 41.0 39.0 41.0 34.0 41.0 18-19 38.21095576526076 41.0 39.0 41.0 34.0 41.0 20-21 38.15945954273509 41.0 39.0 41.0 34.0 41.0 22-23 38.165401746964456 41.0 39.0 41.0 34.0 41.0 24-25 38.12077734883151 41.0 39.0 41.0 34.0 41.0 26-27 38.04421294539289 41.0 39.0 41.0 33.5 41.0 28-29 37.97394658704649 41.0 38.5 41.0 33.0 41.0 30-31 37.857324723011516 41.0 38.0 41.0 33.0 41.0 32-33 37.72442511221951 41.0 38.0 41.0 33.0 41.0 34-35 37.5934414808992 40.5 38.0 41.0 33.0 41.0 36-37 37.51792844254429 40.0 38.0 41.0 32.5 41.0 38-39 37.43873675366419 40.0 38.0 41.0 32.0 41.0 40-41 37.31806499224815 40.0 38.0 41.0 32.0 41.0 42-43 37.15666993073315 40.0 37.5 41.0 31.5 41.0 44-45 36.995582237566424 40.0 37.0 41.0 31.0 41.0 46-47 36.77676227650977 40.0 37.0 41.0 30.5 41.0 48-49 36.567527097638674 40.0 36.5 41.0 30.0 41.0 50-51 36.32124388739045 40.0 36.0 41.0 30.0 41.0 52-53 36.19548551799082 40.0 36.0 41.0 29.5 41.0 54-55 36.070041280546135 40.0 35.0 41.0 29.0 41.0 56-57 35.8760369924195 39.0 35.0 41.0 29.0 41.0 58-59 35.682519683974235 39.0 35.0 41.0 29.0 41.0 60-61 35.42561836274125 39.0 35.0 41.0 28.0 41.0 62-63 35.06742864958581 38.0 35.0 41.0 28.0 41.0 64-65 34.780805034528214 37.0 35.0 40.0 28.0 41.0 66-67 34.47962576212696 37.0 35.0 39.5 28.0 41.0 68-69 34.10651027203977 36.0 35.0 39.0 27.5 41.0 70-71 33.72982998547327 35.5 34.5 39.0 26.5 41.0 72-73 33.404382516527626 35.0 34.0 37.5 26.0 40.0 74-75 33.08200384623767 35.0 34.0 37.0 26.0 39.0 76-77 32.75714969590073 35.0 34.0 36.5 26.0 39.0 78-79 32.492912491705184 35.0 34.0 36.0 26.0 38.0 80-81 32.24630537984791 35.0 34.0 36.0 26.0 37.0 82-83 32.024140063773295 35.0 34.0 35.0 25.5 37.0 84-85 31.81143928798761 35.0 34.0 35.0 25.0 36.0 86-87 31.682393429982767 35.0 34.0 35.0 25.0 36.0 88-89 31.589603134105033 35.0 33.5 35.0 25.0 36.0 90-91 31.48747304890614 35.0 33.0 35.0 24.0 36.0 92-93 31.402153908124667 35.0 33.0 35.0 24.0 35.0 94-95 31.335611666770625 35.0 33.0 35.0 24.0 35.0 96-97 31.275357700854695 35.0 33.0 35.0 23.5 35.0 98-99 31.200153909627694 35.0 33.0 35.0 23.0 35.0 100-101 31.131495035136936 35.0 33.0 35.0 23.0 35.0 102-103 30.44561909922286 34.0 31.5 35.0 20.0 35.0 104-105 30.957845445575135 35.0 33.0 35.0 20.0 35.0 106-107 31.057072440482138 35.0 33.0 35.0 20.5 35.0 108-109 31.04903051213278 35.0 33.0 35.0 20.5 35.0 110-111 30.98475220023883 35.0 33.0 35.0 20.0 35.0 112-113 30.953188702371996 35.0 33.0 35.0 19.5 35.0 114-115 30.827748598242515 35.0 33.0 35.0 19.0 35.0 116-117 31.010684995539776 35.0 33.0 35.0 19.0 35.0 118-119 31.00544808518537 35.0 33.0 35.0 19.0 35.0 120-121 30.93478755887156 35.0 33.0 35.0 18.0 35.0 122-123 30.805528647256118 35.0 33.0 35.0 6.0 35.0 124-125 30.170537954730428 34.5 32.0 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 21092.0 3 5793.0 4 3142.0 5 2394.0 6 2560.0 7 2925.0 8 3285.0 9 3534.0 10 3649.0 11 4104.0 12 4518.0 13 5840.0 14 8074.0 15 7759.0 16 4326.0 17 3895.0 18 3437.0 19 2948.0 20 2846.0 21 2888.0 22 3182.0 23 3863.0 24 5124.0 25 6682.0 26 8044.0 27 9368.0 28 11024.0 29 13528.0 30 16267.0 31 20708.0 32 26640.0 33 36669.0 34 57676.0 35 102807.0 36 234156.0 37 497166.0 38 178446.0 39 292.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.09912723593644 17.856898564385425 11.11366678918888 26.930307410489256 2 15.93430460550147 19.620689810660547 37.65122581401536 26.793779769822624 3 19.051857509498415 23.93908685918591 28.148835307189323 28.860220324126352 4 13.417852433054128 15.03459447090632 34.014810287414114 37.532742808625436 5 14.557384492108044 37.47923466296636 32.04589496535477 15.917485879570817 6 34.30553957521626 33.69640664210583 16.66489942914468 15.333154353533235 7 30.397603596409905 31.837084856837595 18.978295374486933 18.78701617226557 8 28.613852304833102 34.72834740977868 18.111250280050342 18.546550005337874 9 28.504920201769618 15.680972463408583 18.175052434541396 37.63905490028041 10-11 27.21711790839607 25.136823851792585 25.870762182925105 21.775296056886237 12-13 26.92414551242937 22.303429229033462 26.609957106910155 24.162468151627017 14-15 24.45787562588963 23.45250457362808 24.157779453751278 27.93184034673101 16-17 24.60361557929117 26.63903303855124 24.68778711931081 24.06956426284678 18-19 24.400798984805157 25.768350037739022 26.685438449301902 23.145412528153916 20-21 25.145620558939402 24.871217229266783 26.491741591065747 23.491420620728068 22-23 24.899125137437068 24.308756366445742 26.51079317816128 24.28132531795591 24-25 24.309823472183446 24.632012947703018 26.48691344812257 24.571250131990965 26-27 24.080993080228392 24.788827659546484 26.640124905353378 24.49005435487174 28-29 24.27814085760323 25.501468519275633 26.31449009162839 23.905900531492748 30-31 25.04682027926831 24.920262734665044 26.361620898528503 23.671296087538142 32-33 24.559754655358947 24.781432151617036 26.50348186614948 24.155331326874535 34-35 24.500616643281067 25.14525500322789 26.482217300432666 23.87191105305838 36-37 24.634873155388874 25.070477579073554 26.435874985438517 23.858774280099055 38-39 24.674015357473664 25.16010363570434 26.349246311406876 23.816634695415114 40-41 24.577495617603237 24.805361460836497 26.49571718181357 24.121425739746694 42-43 24.50485590120228 24.97359269050182 26.114771747278947 24.40677966101695 44-45 24.12051841859032 25.10869279301961 26.459376890615783 24.31141189777429 46-47 24.209015706578064 24.991150535589878 26.11508888169074 24.684744876141316 48-49 24.302319813861768 25.2742322399115 25.898863991630993 24.524583954595737 50-51 24.407412598501534 25.421333484640506 25.944808704771777 24.226445212086187 52-53 24.56260594084109 25.390254964447468 25.70202305438037 24.345116040331074 54-55 24.21171653585915 24.947297458779286 26.110060191750822 24.730925813610746 56-57 24.36303063870913 25.254934290869713 25.675259293105483 24.706775777315677 58-59 24.373965251229297 25.335159025338992 25.97289869438472 24.317977029046993 60-61 24.707980842431024 25.242832632797978 26.017680094450153 24.031506430320842 62-63 24.654272857934636 25.27743280946163 25.96612525950907 24.10216907309466 64-65 24.354207076085316 24.902961031855835 25.994795028899386 24.748036863159463 66-67 24.341194273146456 25.118733271281524 25.933473294361185 24.60659916121083 68-69 24.14090470478956 25.138767393833362 26.11393430549418 24.606393595882896 70-71 24.180870866966618 25.059763980187142 26.16955911054063 24.58980604230561 72-73 24.234982985426388 25.590211810398728 25.631846411909 24.542958792265885 74-75 24.191801638830498 25.464589582160603 25.69520277953348 24.64840599947542 76-77 24.158008454673556 25.025157350868955 25.83015500234852 24.98667919210897 78-79 24.095581966236765 25.03925717422745 26.135339272542673 24.729821586993108 80-81 24.208601654378196 25.40403156049182 25.69873693402703 24.688629851102956 82-83 24.45533865258954 25.369567806988634 25.664177391044245 24.510916149377582 84-85 24.4218501996056 25.51735125775576 25.39812148285316 24.662677059785484 86-87 23.97281481019441 25.702832673631175 25.672434019138002 24.651918497036412 88-89 23.857658776815704 25.3128678933756 25.781103511973807 25.048369817834885 90-91 24.36100153535487 25.14070328722259 25.719598958994826 24.778696218427715 92-93 24.30558425913331 25.485345143091614 25.66754919208718 24.541521405687895 94-95 24.08670475703767 25.198753201075448 25.676053982861212 25.03848805902567 96-97 23.929137485692902 25.165842121757088 25.836913709490144 25.068106683059867 98-99 23.83115851003029 25.269934982634357 25.56415331067013 25.334753196665226 100-101 23.73916977479444 25.37810440152978 25.71068597250519 25.17203985117059 102-103 23.662499483329388 25.211759798893006 25.802229762481822 25.32351095529578 104-105 24.021850958666096 25.23193534593218 25.64519171443151 25.101021980970216 106-107 23.921346119160706 25.148838153069196 25.749113208681536 25.18070251908856 108-109 23.903891130123192 25.050765961028574 25.754274711862777 25.291068196985456 110-111 23.732039329636144 25.481258978221554 25.71084593587873 25.07585575626357 112-113 23.743345809724822 25.527177954475512 25.653319645751143 25.07615659004852 114-115 23.716661994767975 25.602205236384297 25.56395328301711 25.117179485830622 116-117 23.54414607238086 25.35967979029451 25.660026694162458 25.436147443162177 118-119 23.61563854511704 25.238976494084536 25.766023045604598 25.379361915193826 120-121 23.473026936050974 25.27775700625538 25.974202144948823 25.275013912744825 122-123 23.36769049247349 25.462863455656343 25.668405191525817 25.501040860344347 124-125 23.271896854725135 25.45751983100016 25.59090512416192 25.67967819011279 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 25.0 1 18.5 2 16.0 3 47.0 4 63.0 5 69.5 6 153.5 7 411.5 8 729.5 9 841.0 10 733.0 11 587.5 12 574.5 13 669.0 14 723.0 15 731.5 16 775.5 17 834.0 18 899.0 19 988.5 20 1011.5 21 1062.5 22 1216.5 23 1367.5 24 1545.0 25 1819.0 26 2256.5 27 2828.0 28 3506.0 29 4395.0 30 5688.0 31 7261.5 32 9204.5 33 11236.0 34 13257.0 35 15466.5 36 18100.5 37 20920.0 38 23285.5 39 25685.5 40 28141.0 41 30433.0 42 32776.5 43 36534.0 44 40645.0 45 44212.5 46 47547.5 47 50746.0 48 55303.5 49 59416.0 50 62296.5 51 63858.0 52 63203.5 53 61902.5 54 60902.5 55 58530.5 56 54522.0 57 49923.5 58 45435.5 59 40689.0 60 34703.0 61 29190.5 62 23975.0 63 18376.5 64 13528.0 65 10013.5 66 7633.0 67 5907.0 68 4626.0 69 3667.0 70 2841.0 71 2089.5 72 1601.0 73 1288.0 74 1022.5 75 796.0 76 574.5 77 344.5 78 190.5 79 101.5 80 66.5 81 41.0 82 21.0 83 9.0 84 5.5 85 6.0 86 6.5 87 6.5 88 3.5 89 1.5 90 1.5 91 1.5 92 1.5 93 0.5 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0210423319112224 2 0.0018787796349305718 3 0.0322398585354086 4 0.15894475711512634 5 0.023221716287741865 6 0.0670348573743228 7 0.04944947999137265 8 0.04005558181671979 9 0.03163864905223083 10-11 0.06305184454826998 12-13 0.05384582433711018 14-15 0.0559124819355338 16-17 0.04727009561485318 18-19 0.03479499883891419 20-21 0.02329686747313909 22-23 0.0031563497866833605 24-25 0.005072705014312544 26-27 0.0032690765647791944 28-29 0.004433919938436149 30-31 0.0023296867473139088 32-33 0.008604810727982018 34-35 0.004509071123833371 36-37 0.006463001944161166 38-39 0.002141808783820852 40-41 0.0032690765647791944 42-43 0.004020588418751424 44-45 0.004959978236216709 46-47 0.0046593734946278175 48-49 0.0020290820057250174 50-51 0.00184120404223196 52-53 0.0010896921882597315 54-55 0.0052605829778056 56-57 0.0010521165955611202 58-59 0.0010521165955611202 60-61 1.503023707944457E-4 62-63 0.0010145410028625087 64-65 0.0 66-67 0.0031939253793819714 68-69 5.260582977805601E-4 70-71 0.0 72-73 0.0020290820057250174 74-75 0.005110280607011154 76-77 0.0019539308203277944 78-79 1.8787796349305715E-4 80-81 0.0 82-83 0.005749065682887549 84-85 0.0020290820057250174 86-87 0.0 88-89 0.0013151457444514 90-91 0.0010521165955611202 92-93 0.0 94-95 0.0032690765647791944 96-97 0.0013527213371500116 98-99 4.884827050819486E-4 100-101 0.0 102-103 0.0012024189663555657 104-105 0.0 106-107 8.266630393694515E-4 108-109 0.0026302914889028 110-111 3.757559269861143E-5 112-113 3.381803342875029E-4 114-115 0.0 116-117 0.001728477264136126 118-119 0.002141808783820852 120-121 0.0027430182669986344 122-123 0.0015781748933416803 124-125 0.10663953207865925 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1330651.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.82878738586115 #Duplication Level Percentage of deduplicated Percentage of total 1 80.65906691115934 49.8705229879202 2 10.678840399132557 13.20519505131023 3 3.4034084220393197 6.312858471405549 4 1.5993703522022087 3.955485178302409 5 0.92038464076596 2.845313313356537 6 0.595875127494251 2.2105341939818968 7 0.4203759711569268 1.8193935579950569 8 0.2948821352070999 1.458576387328683 9 0.2163087205572975 1.203669530374032 >10 1.1373420789872346 12.314717981050022 >50 0.05452993068185143 2.2629202367949177 >100 0.01876248589173039 1.8881019498643574 >500 4.873284138134513E-4 0.19457044744334315 >1k 3.654963103600885E-4 0.4581407128727886 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2534 0.19043310379656272 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2126 0.1597714201544958 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1422 0.10686498563485092 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 7.515118539722287E-5 0.0 10-11 0.0 0.0 0.0 7.515118539722287E-5 0.0 12-13 0.0 0.0 0.0 7.515118539722287E-5 3.381803342875029E-4 14-15 0.0 3.7575592698611434E-5 0.0 7.515118539722287E-5 4.1333151968472577E-4 16-17 0.0 7.515118539722287E-5 0.0 1.127267780958343E-4 4.509071123833372E-4 18-19 0.0 7.515118539722287E-5 0.0 1.5030237079444574E-4 5.2605829778056E-4 20-21 0.0 7.515118539722287E-5 0.0 1.5030237079444574E-4 5.2605829778056E-4 22-23 0.0 7.515118539722287E-5 0.0 1.5030237079444574E-4 5.636338904791715E-4 24-25 0.0 7.515118539722287E-5 0.0 3.381803342875029E-4 6.763606685750058E-4 26-27 0.0 7.515118539722287E-5 0.0 5.636338904791715E-4 6.763606685750058E-4 28-29 0.0 7.515118539722287E-5 0.0 0.001728477264136126 6.763606685750058E-4 30-31 0.0 7.515118539722287E-5 0.0 0.006387850758763944 6.763606685750058E-4 32-33 0.0 7.515118539722287E-5 0.0 0.01803628449533349 6.763606685750058E-4 34-35 0.0 7.515118539722287E-5 0.0 0.0325404632769975 8.266630393694515E-4 36-37 0.0 7.515118539722287E-5 0.0 0.05429673144949352 8.266630393694515E-4 38-39 0.0 1.127267780958343E-4 0.0 0.09352565022684385 8.266630393694515E-4 40-41 0.0 1.5030237079444574E-4 0.0 0.1462817823756943 8.266630393694515E-4 42-43 0.0 1.5030237079444574E-4 0.0 0.19009492346227524 8.64238632068063E-4 44-45 0.0 1.5030237079444574E-4 0.0 0.2349601811444173 9.018142247666744E-4 46-47 0.0 1.5030237079444574E-4 0.0 0.2869272258465969 9.018142247666744E-4 48-49 0.0 1.5030237079444574E-4 0.0 0.34268940541133625 0.00105211659556112 50-51 0.0 1.5030237079444574E-4 0.0 0.4083339658558104 0.00105211659556112 52-53 0.0 1.5030237079444574E-4 0.0 0.47961486520507635 0.0011272677809583429 54-55 0.0 2.254535561916686E-4 0.0 0.5558181671978604 0.0011272677809583429 56-57 0.0 2.254535561916686E-4 0.0 0.6342008535671637 0.0011272677809583429 58-59 0.0 2.254535561916686E-4 0.0 0.7306574000244993 0.0011272677809583429 60-61 0.0 2.254535561916686E-4 0.0 0.8288048481532724 0.0011272677809583429 62-63 0.0 2.254535561916686E-4 0.0 0.9509631000164581 0.0011272677809583429 64-65 0.0 2.254535561916686E-4 0.0 1.0568135446484463 0.0011272677809583429 66-67 0.0 3.006047415888915E-4 0.0 1.1687888108903086 0.0011272677809583429 68-69 0.0 3.006047415888915E-4 0.0 1.2982743033297237 0.0011272677809583429 70-71 0.0 3.006047415888915E-4 0.0 1.4421888233654054 0.0011272677809583429 72-73 0.0 3.006047415888915E-4 0.0 1.5730270371419703 0.0013151457444514 74-75 0.0 3.006047415888915E-4 0.0 1.7054058502191785 0.0014278725225472344 76-77 0.0 3.006047415888915E-4 0.0 1.871189365205452 0.0014278725225472344 78-79 0.0 3.006047415888915E-4 0.0 2.0596309625889884 0.0014278725225472344 80-81 0.0 3.006047415888915E-4 0.0 2.2362738238651607 0.0014278725225472344 82-83 0.0 3.006047415888915E-4 0.0 2.417275453894372 0.0015030237079444572 84-85 0.0 3.006047415888915E-4 0.0 2.616275792826218 0.0015030237079444572 86-87 0.0 3.006047415888915E-4 0.0 2.8498081014480885 0.00157817489334168 88-89 0.0 3.006047415888915E-4 0.0 3.1145657276025043 0.001653326078738903 90-91 0.0 3.006047415888915E-4 0.0 3.384283331993137 0.0016909016714375144 92-93 0.0 3.006047415888915E-4 0.0 3.636791314927806 0.0018787796349305716 94-95 0.0 3.006047415888915E-4 0.0 3.8989186495933197 0.001916355227629183 96-97 0.0 3.006047415888915E-4 0.0 4.202454287412703 0.00210423319112224 98-99 0.0 3.006047415888915E-4 0.0 4.543790971486889 0.0022545355619166857 100-101 0.0 3.006047415888915E-4 0.0 4.903013637685614 0.0022545355619166857 102-103 0.0 3.006047415888915E-4 0.0 5.307627619864261 0.0022545355619166857 104-105 0.0 3.006047415888915E-4 0.0 5.707582228548282 0.0022545355619166857 106-107 0.0 3.006047415888915E-4 0.0 6.127451901362566 0.0022545355619166857 108-109 0.0 3.006047415888915E-4 0.0 6.5611493922899395 0.0022545355619166857 110-111 0.0 3.006047415888915E-4 0.0 7.070975034024699 0.0022545355619166857 112-113 0.0 3.006047415888915E-4 0.0 7.55209292293772 0.0022545355619166857 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2450 0.0 46.722385 1 GTATCAA 3865 0.0 45.19683 1 GTACATG 7470 0.0 34.239437 1 TATCAAC 5085 0.0 34.000126 2 TCAACGC 5080 0.0 33.79379 4 CAACGCA 5185 0.0 33.316788 5 ATCAACG 5155 0.0 33.307137 3 TACATGG 7765 0.0 32.9374 2 ACATGGG 7745 0.0 32.483627 3 AACGCAG 5405 0.0 31.860071 6 CATGGGG 6460 0.0 31.649706 4 ATGGGGG 4035 0.0 31.297247 5 TGGGGGG 3060 0.0 27.066866 6 ACGCAGA 6360 0.0 26.980331 7 CGCAGAG 6655 0.0 25.783388 8 TAACGCA 125 0.0016164295 23.843393 4 GCAGAGT 7380 0.0 23.331198 9 TATGTAA 625 0.0 22.893108 2 AGAGTAC 6825 0.0 22.042168 10-11 GAGTACT 3380 0.0 22.01513 12-13 >>END_MODULE