FastQCFastQC Report
Mon 15 Aug 2016
SRR2921644_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921644_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1762120
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34600.050.7525861
GGTATCA20250.050.2670441
TCAACGC41200.041.4582144
CAACGCA42250.040.2858855
TATCAAC44700.038.7446172
ATCAACG44950.038.396733
AACGCAG45100.037.9975436
GTACATG93400.037.156431
TACATGG95350.036.01482
ACATGGG98550.034.0602953
CATGGGG86400.032.7883154
ACGCAGA52850.032.303777
ATGGGGG53550.031.2291685
CGCAGAG57900.029.279948
TATACCG2103.1322998E-928.3396365
GCAGAGT65400.025.2847489
TGGGGGG43500.024.0748166
GAGTACA67850.022.810551
AGTACAT67150.022.2460862
CTATACG1350.002552098122.0425664
Produced by FastQC (version 0.11.5)