##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921644_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1762120
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.76441559031167	31.0	31.0	34.0	26.0	34.0
2	29.924545433909156	31.0	31.0	34.0	26.0	34.0
3	30.207458629378248	31.0	31.0	34.0	26.0	34.0
4	33.67731709531701	37.0	35.0	37.0	28.0	37.0
5	33.75999023903026	37.0	35.0	37.0	30.0	37.0
6	33.668777381790115	37.0	35.0	37.0	29.0	37.0
7	33.63293532790048	37.0	35.0	37.0	28.0	37.0
8	33.49668751276871	37.0	35.0	37.0	28.0	37.0
9	35.173530747054684	39.0	37.0	39.0	28.0	39.0
10-11	35.202594318207616	39.0	37.0	39.0	28.0	39.0
12-13	35.131222901959006	39.0	37.0	39.0	27.0	39.0
14-15	36.41947653962273	40.0	38.0	41.0	27.0	41.0
16-17	36.38252843166187	40.0	38.0	41.0	27.0	41.0
18-19	36.35107001793294	40.0	38.0	41.0	27.0	41.0
20-21	36.40301057816721	40.0	38.0	41.0	27.0	41.0
22-23	36.34027960638322	40.0	38.0	41.0	27.0	41.0
24-25	36.32887204049668	40.0	38.0	41.0	27.0	41.0
26-27	36.27986289242503	40.0	38.0	41.0	27.0	41.0
28-29	36.22169120150727	40.0	37.0	41.0	27.0	41.0
30-31	36.12516003450389	40.0	37.0	41.0	27.0	41.0
32-33	36.018522858829144	40.0	37.0	41.0	27.0	41.0
34-35	35.89263585907884	40.0	37.0	41.0	26.0	41.0
36-37	35.79548668649127	40.0	37.0	41.0	25.0	41.0
38-39	35.6843308060745	40.0	36.0	41.0	25.0	41.0
40-41	35.56462641590811	40.0	36.0	41.0	24.5	41.0
42-43	35.351075976664475	40.0	35.5	41.0	24.0	41.0
44-45	35.07033488071187	40.0	35.0	41.0	23.0	41.0
46-47	34.83363817447166	39.5	35.0	41.0	22.5	41.0
48-49	34.648569336935054	39.0	35.0	41.0	21.5	41.0
50-51	34.45327332985268	39.0	35.0	41.0	20.0	41.0
52-53	34.25076981136358	39.0	34.0	41.0	19.5	41.0
54-55	34.00944742696298	38.0	34.0	40.0	18.0	41.0
56-57	33.76399365536967	38.0	34.0	40.0	16.5	41.0
58-59	33.76784186094024	38.0	34.0	40.0	17.5	41.0
60-61	33.55241612376002	37.5	34.0	40.0	14.5	41.0
62-63	33.24699821805552	37.0	34.0	40.0	13.0	41.0
64-65	32.946668217828524	36.5	33.0	39.5	9.5	41.0
66-67	32.623883163462196	36.0	33.0	39.0	7.5	41.0
68-69	32.291966211154744	35.5	33.0	39.0	7.0	41.0
70-71	31.927875797335027	35.0	33.0	38.5	3.5	40.0
72-73	31.5871546773205	35.0	32.0	37.0	2.0	39.5
74-75	31.22463963861712	35.0	32.0	37.0	2.0	39.0
76-77	30.93011769913513	35.0	32.0	36.0	2.0	39.0
78-79	30.663980886659253	35.0	32.0	36.0	2.0	37.5
80-81	30.412123748666378	35.0	32.0	35.5	2.0	37.0
82-83	30.196402628651853	35.0	31.0	35.0	2.0	37.0
84-85	29.99051001066897	35.0	31.0	35.0	2.0	36.0
86-87	29.832937030395208	35.0	31.0	35.0	2.0	36.0
88-89	29.67952891971035	35.0	31.0	35.0	2.0	36.0
90-91	29.507819842008487	35.0	31.0	35.0	2.0	35.0
92-93	29.44202977095771	34.5	31.0	35.0	2.0	35.0
94-95	29.33089063173904	34.0	31.0	35.0	2.0	35.0
96-97	29.252333836515106	34.0	30.5	35.0	2.0	35.0
98-99	29.180020940685083	34.0	30.0	35.0	2.0	35.0
100-101	29.08797414478015	34.0	30.0	35.0	2.0	35.0
102-103	28.257545172859963	33.5	29.0	34.5	2.0	35.0
104-105	29.156997253308514	34.0	30.5	35.0	2.0	35.0
106-107	29.424536353937302	35.0	31.0	35.0	2.0	35.0
108-109	29.43634627607654	35.0	31.0	35.0	2.0	35.0
110-111	29.389208737202914	35.0	31.0	35.0	2.0	35.0
112-113	29.328761662088848	35.0	31.0	35.0	2.0	35.0
114-115	29.234547874151588	35.0	31.0	35.0	2.0	35.0
116-117	29.13549417746805	35.0	31.0	35.0	2.0	35.0
118-119	28.88352495857263	35.0	31.0	35.0	2.0	35.0
120-121	28.794460082173746	35.0	31.0	35.0	2.0	35.0
122-123	28.586299457471682	35.0	30.0	35.0	2.0	35.0
124-125	27.86006571629628	34.0	28.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	79356.0
3	13495.0
4	6703.0
5	5544.0
6	5829.0
7	6335.0
8	6383.0
9	6653.0
10	7116.0
11	7588.0
12	7903.0
13	7826.0
14	8055.0
15	6862.0
16	5836.0
17	5218.0
18	4907.0
19	4597.0
20	4583.0
21	4780.0
22	5312.0
23	7333.0
24	10906.0
25	13793.0
26	14749.0
27	16155.0
28	18727.0
29	22735.0
30	27429.0
31	34220.0
32	44248.0
33	61643.0
34	97269.0
35	163426.0
36	333480.0
37	536084.0
38	148950.0
39	92.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.84340187605546	17.549377722905756	12.676231427476692	24.930988973562094
2	16.932425920849433	19.096980418665183	38.7408034244208	25.2297902360646
3	20.119719764714965	23.553078751472803	27.85485156202113	28.472349921791107
4	13.811997284798732	14.997230313088133	34.2181764108944	36.97259599121873
5	14.808188730392613	37.37073144151896	32.594293680002	15.226786148086427
6	34.247253864584195	33.36311697872277	16.908457608575873	15.481171548117153
7	30.16432371413954	31.471463525291338	19.77801336326165	18.586199397307475
8	27.78222223483956	34.2636600813629	19.35999818310343	18.594119500694113
9	27.88935966340379	16.154302023800604	18.264442824737912	37.69189548805769
10-11	26.797424958011916	25.41752450298487	26.090015182709198	21.69503535629401
12-13	26.528088841767346	22.786703248926994	26.510352045605984	24.174855863699673
14-15	24.272758984483765	24.055939848087526	23.71976942637303	27.95153174105568
16-17	24.64333755604939	27.12289409422457	24.291195395754027	23.942572953972014
18-19	24.64785653780383	26.40295317925507	25.74527806985073	23.20391221309037
20-21	25.510861537605667	25.212748987700266	25.474360277091684	23.802029197602383
22-23	25.398889892480685	24.748573761064034	25.40800169859962	24.444534647855658
24-25	24.84271615051451	25.09561921370706	25.3619479950691	24.699716640709333
26-27	24.570233190863135	25.334837143389215	25.532985787802676	24.561943877944977
28-29	24.651510575047293	25.80331979042943	25.325977848381555	24.219191786141725
30-31	25.266212132995093	25.276514921446203	25.346392235954855	24.110880709603848
32-33	24.771980138273676	25.279053621779095	25.367837604785255	24.581128635161978
34-35	24.80148388876966	25.57411552030922	25.4343054462624	24.190095144658716
36-37	24.904856375765032	25.642366673354367	25.26792177833373	24.184855172546875
38-39	24.926748139612766	25.668859586883347	25.323091506644037	24.081300766859847
40-41	24.914136512371183	25.396345972442496	25.411589775354038	24.277927739832283
42-43	24.699878129149045	25.392050069790507	25.3056279130227	24.602443888037755
44-45	24.557144519399138	25.463147170089766	25.56114325932779	24.418565051183304
46-47	24.53879303983352	25.43083825571244	25.383922864625962	24.646445839828075
48-49	24.452341350711574	25.723607649907187	25.375683331535715	24.448367667845524
50-51	24.515341442244868	25.63398295073629	25.503107684430244	24.3475679225886
52-53	24.658489913780237	25.56408412288176	25.331349220990873	24.446076742347138
54-55	24.405068866895558	25.26980400415395	25.64018583234163	24.684941296608866
56-57	24.548085609781086	25.524900171402347	25.24294771164103	24.68406650717554
58-59	24.5027419610719	25.752382595734503	25.405887716814725	24.33898772637888
60-61	24.834577490731967	25.539152217090187	25.50977719828734	24.116493093890508
62-63	24.735800191577678	25.516699187568737	25.493057083052474	24.254443537801116
64-65	24.549799161795303	25.270790294716903	25.47084802168763	24.70856252180016
66-67	24.519931482645724	25.561968610877344	25.369245812394713	24.548854094082216
68-69	24.470377677060867	25.51487807113967	25.47639259387292	24.538351657926547
70-71	24.367412915126845	25.39747652159003	25.62338932799031	24.611721235292816
72-73	24.518379596176214	25.73679875620775	25.291151943936256	24.45366970367978
74-75	24.461566359117327	25.653434751874283	25.33705044923506	24.54794843977333
76-77	24.39098315364857	25.275401704642686	25.421470960257075	24.912144181451673
78-79	24.374923013084796	25.319995935658525	25.61519995595071	24.689881095305967
80-81	24.53252680306428	25.625622415441608	25.278531353561483	24.56331942793263
82-83	24.54668504704224	25.6675871045601	25.42193832356557	24.363789524832097
84-85	24.565514935854193	25.70358613178106	25.181673373027575	24.549225559337177
86-87	24.317665683004094	25.888659386541928	25.314014268499584	24.479660661954394
88-89	24.249396611031518	25.547562717259765	25.42779312897198	24.775247542736743
90-91	24.546310628855956	25.413870398467864	25.396132082058305	24.64368689061788
92-93	24.51551388502642	25.607019415767578	25.45678984318689	24.420676856019117
94-95	24.500615105632154	25.424536400489817	25.404926804558166	24.669921689319864
96-97	24.2802378433358	25.44357237239419	25.506489835580236	24.769699948689773
98-99	24.129335656864306	25.505604936737132	25.341470799645965	25.0235886067526
100-101	24.152505697637427	25.579816727769707	25.54142099403802	24.726256580554846
102-103	23.9895008654692	25.53188048012258	25.60338809908913	24.87523055531909
104-105	24.111586036677572	25.565817390788638	25.507390479336195	24.81520609319759
106-107	24.122246356969466	25.449868693417933	25.535193143398704	24.8926918062139
108-109	24.102495264939208	25.482970524849634	25.55000207167778	24.864532138533377
110-111	23.979010140804466	25.842480147143533	25.506628779817593	24.671880932234412
112-113	23.90083517940339	25.882190699438983	25.54145634426201	24.67551777689561
114-115	24.006958505794262	25.904967584203735	25.516630743711595	24.57144316629041
116-117	23.909017051924785	25.728029021558168	25.531859951231567	24.83109397528548
118-119	23.927793240375646	25.589474001685552	25.609447972926446	24.873284785012352
120-121	23.87673965852511	25.722812918625394	25.705702569928974	24.694744852920522
122-123	23.81589606734001	25.806302414303367	25.605945170612948	24.771856347743675
124-125	23.769334635781345	25.64920316139653	25.67345328606267	24.908008916759456
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	413.0
1	333.5
2	240.5
3	200.0
4	176.5
5	170.5
6	161.5
7	173.0
8	209.0
9	239.0
10	252.5
11	282.0
12	309.5
13	351.5
14	403.0
15	435.5
16	465.0
17	526.5
18	573.0
19	625.0
20	722.0
21	789.0
22	954.5
23	1224.0
24	1478.0
25	1860.5
26	2447.5
27	3259.5
28	4416.0
29	5890.5
30	7695.5
31	9873.0
32	12592.0
33	15163.0
34	17785.5
35	20959.0
36	24540.0
37	28212.0
38	31459.5
39	35341.5
40	39323.0
41	42775.5
42	46315.0
43	51050.5
44	56525.0
45	61639.0
46	65382.0
47	68876.0
48	73733.0
49	77651.0
50	79550.5
51	80132.0
52	79661.5
53	78338.5
54	77661.5
55	75456.0
56	70462.5
57	64804.0
58	59466.0
59	53606.5
60	45867.0
61	38770.0
62	32517.5
63	25327.5
64	18835.0
65	14391.5
66	11280.5
67	8917.5
68	7070.5
69	5469.0
70	4270.0
71	3238.0
72	2465.5
73	2070.5
74	1655.5
75	1241.5
76	936.5
77	647.0
78	414.0
79	298.0
80	213.0
81	142.5
82	106.0
83	92.5
84	75.0
85	56.0
86	44.5
87	36.0
88	37.0
89	34.0
90	28.0
91	27.5
92	25.5
93	24.0
94	24.0
95	20.0
96	12.5
97	10.5
98	9.0
99	6.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025253671713617688
2	0.010612217102126982
3	0.009760969740993804
4	0.010668966926202528
5	0.016116950037454884
6	0.09341021042834767
7	0.05805507002928292
8	0.04965609606610219
9	0.027466914852563955
10-11	0.0514153406124441
12-13	0.013960456722584159
14-15	0.029141034662792546
16-17	0.03492951671849817
18-19	0.043356865593716654
20-21	0.03047465552856786
22-23	0.03796563230653985
24-25	0.03265952375547636
26-27	0.046307856445645015
28-29	0.045626858556738474
30-31	0.02616166889882641
32-33	0.030701654824870042
34-35	0.04551335890858738
36-37	0.010129843597484848
38-39	0.022132431389462692
40-41	0.042477243320545706
42-43	0.02377817628765351
44-45	0.01787619458379679
46-47	0.024856422945088868
48-49	0.030077406760039043
50-51	0.029453158695208047
52-53	0.026048169250675324
54-55	0.024090300320069008
56-57	0.02698454134792182
58-59	0.01892606632919438
60-61	0.02377817628765351
62-63	0.02454429891267337
64-65	0.02147980841259392
66-67	0.02965178307947245
68-69	0.023636301727464643
70-71	0.046591605566022744
72-73	0.023324177695049145
74-75	0.010243343245635939
76-77	0.016145324949492657
78-79	0.02593466960252423
80-81	0.018982816153269924
82-83	0.02601979433863755
84-85	0.013364583569790934
86-87	0.01912469071345879
88-89	0.02292692892652033
90-91	0.02252968015799151
92-93	0.008512473611331806
94-95	0.01291058497718657
96-97	0.016343949333757064
98-99	0.008313849227067396
100-101	0.011662088847524572
102-103	0.003972487685288175
104-105	0.00507910925476131
106-107	0.0012768710416997708
108-109	0.01492520373186843
110-111	0.0017592445463419065
112-113	0.0024686173472862234
114-115	0.014045581458697477
116-117	0.006781603977027671
118-119	0.27427189975711075
120-121	0.001617369986153043
122-123	9.931219213220439E-4
124-125	0.30803804508206023
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1762120.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.65717328637108
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.43773957759556	53.30191213205329
2	9.653269285435618	12.483062099368286
3	3.112166676630907	6.036717003209825
4	1.4882558920502162	3.8490567642701445
5	0.8226258950889755	2.6594332524312003
6	0.5406196288242163	2.097296221374058
7	0.38608374567852244	1.7474158553171213
8	0.2704672073003663	1.3990116072560506
9	0.19801946652854954	1.1523041065270068
>10	1.0288660243869465	11.54597147639351
>50	0.04739729740071447	2.0413811723389936
>100	0.01413816030977302	1.5224026398200303
>500	2.63357077314728E-4	0.10211078535750312
>1k	8.778569243824262E-5	0.061924884282954895
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	5.6749824075545367E-5	5.6749824075545367E-5
10-11	0.0	0.0	0.0	5.6749824075545367E-5	5.6749824075545367E-5
12-13	0.0	0.0	0.0	5.6749824075545367E-5	8.512473611331805E-5
14-15	0.0	0.0	0.0	5.6749824075545367E-5	1.986243842644088E-4
16-17	0.0	0.0	0.0	5.6749824075545367E-5	2.2699929630218147E-4
18-19	0.0	0.0	0.0	5.6749824075545367E-5	2.2699929630218147E-4
20-21	0.0	0.0	0.0	8.512473611331805E-5	2.2699929630218147E-4
22-23	0.0	0.0	0.0	2.553742083399542E-4	2.8374912037772685E-4
24-25	0.0	2.8374912037772683E-5	0.0	3.688738564910449E-4	2.8374912037772685E-4
26-27	0.0	5.6749824075545367E-5	0.0	5.674982407554537E-4	2.8374912037772685E-4
28-29	0.0	5.6749824075545367E-5	0.0	0.0020146187546818605	2.8374912037772685E-4
30-31	0.0	5.6749824075545367E-5	0.0	0.007689601162236397	3.1212403241549953E-4
32-33	0.0	5.6749824075545367E-5	0.0	0.02054343631534742	3.404989444532722E-4
34-35	0.0	1.1349964815109073E-4	0.0	0.03649013688057567	5.107484166799082E-4
36-37	0.0	1.1349964815109073E-4	0.0	0.05933194107098268	5.107484166799082E-4
38-39	0.0	1.1349964815109073E-4	0.0	0.09812044582661794	5.107484166799082E-4
40-41	0.0	1.1349964815109073E-4	0.0	0.14474042630467845	5.107484166799082E-4
42-43	0.0	1.1349964815109073E-4	0.0	0.18724604453726193	5.107484166799082E-4
44-45	0.0	1.1349964815109073E-4	0.0	0.23897350918212154	5.107484166799082E-4
46-47	0.0	1.1349964815109073E-4	0.0	0.2940208385354005	5.39123328717681E-4
48-49	0.0	1.1349964815109073E-4	0.0	0.35145166049985244	5.674982407554537E-4
50-51	0.0	1.1349964815109073E-4	0.0	0.41274147050144144	6.242480648309991E-4
52-53	0.0	1.1349964815109073E-4	0.0	0.4828558781467777	6.242480648309991E-4
54-55	0.0	1.1349964815109073E-4	0.0	0.5560064013801558	6.242480648309991E-4
56-57	0.0	1.1349964815109073E-4	0.0	0.6327889133543686	6.242480648309991E-4
58-59	0.0	1.1349964815109073E-4	0.0	0.7206943908473884	6.242480648309991E-4
60-61	0.0	1.1349964815109073E-4	0.0	0.8128561051460741	6.242480648309991E-4
62-63	0.0	1.1349964815109073E-4	0.0	0.9206807708896103	7.944975370576351E-4
64-65	0.0	1.1349964815109073E-4	0.0	1.0220359566885344	7.944975370576351E-4
66-67	0.0	1.1349964815109073E-4	0.0	1.1409835879508774	7.944975370576351E-4
68-69	0.0	1.1349964815109073E-4	0.0	1.2653792023244728	7.944975370576351E-4
70-71	0.0	1.1349964815109073E-4	0.0	1.389065443897124	7.944975370576351E-4
72-73	0.0	1.1349964815109073E-4	0.0	1.5177172950763853	9.931219213220439E-4
74-75	0.0	1.1349964815109073E-4	0.0	1.655165368987356	0.001078246657435362
76-77	0.0	1.1349964815109073E-4	0.0	1.8229178489546682	0.001078246657435362
78-79	0.0	1.1349964815109073E-4	0.0	2.007184527727964	0.0011349964815109072
80-81	0.0	1.1349964815109073E-4	0.0	2.1792499943250174	0.0012484961296619981
82-83	0.0	1.1349964815109073E-4	0.0	2.362353301704765	0.0013336208657753162
84-85	0.0	1.1349964815109073E-4	0.0	2.562311306834949	0.0013619957778130889
86-87	0.0	1.1349964815109073E-4	0.0	2.781848001271196	0.0013619957778130889
88-89	0.0	1.1349964815109073E-4	0.0	3.031121603523029	0.0013619957778130889
90-91	0.0	1.1349964815109073E-4	0.0	3.2925680430390667	0.0013619957778130889
92-93	0.0	1.1349964815109073E-4	0.0	3.5427780173881462	0.001418745601888634
94-95	0.0	1.1349964815109073E-4	0.0	3.8049622046171656	0.0014754954259641796
96-97	0.0	1.1349964815109073E-4	0.0	4.100430163666493	0.0015606201620774977
98-99	0.0	1.1349964815109073E-4	0.0	4.4345447529112665	0.0016457448981908155
100-101	0.0	1.1349964815109073E-4	0.0	4.797261253490114	0.0016457448981908155
102-103	0.0	1.1349964815109073E-4	0.0	5.161453249494927	0.001872744194492997
104-105	0.0	1.702494722266361E-4	0.0	5.544259187796518	0.0019294940185685425
106-107	0.0	1.702494722266361E-4	0.0	5.959043651964679	0.0019294940185685425
108-109	0.0	1.702494722266361E-4	0.0	6.413921866842212	0.0019862438426440877
110-111	0.0	1.702494722266361E-4	0.0	6.89984223548907	0.002042993666719633
112-113	0.0	1.702494722266361E-4	0.0	7.369078155857717	0.002042993666719633
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3460	0.0	50.752586	1
GGTATCA	2025	0.0	50.267044	1
TCAACGC	4120	0.0	41.458214	4
CAACGCA	4225	0.0	40.285885	5
TATCAAC	4470	0.0	38.744617	2
ATCAACG	4495	0.0	38.39673	3
AACGCAG	4510	0.0	37.997543	6
GTACATG	9340	0.0	37.15643	1
TACATGG	9535	0.0	36.0148	2
ACATGGG	9855	0.0	34.060295	3
CATGGGG	8640	0.0	32.788315	4
ACGCAGA	5285	0.0	32.30377	7
ATGGGGG	5355	0.0	31.229168	5
CGCAGAG	5790	0.0	29.27994	8
TATACCG	210	3.1322998E-9	28.339636	5
GCAGAGT	6540	0.0	25.284748	9
TGGGGGG	4350	0.0	24.074816	6
GAGTACA	6785	0.0	22.81055	1
AGTACAT	6715	0.0	22.246086	2
CTATACG	135	0.0025520981	22.042566	4
>>END_MODULE