FastQCFastQC Report
Mon 15 Aug 2016
SRR2921644_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921644_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1762120
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27060.15356502394842578No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23000.13052459537375433No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27600.065.492611
GTATCAA49400.052.2901271
TCAACGC63950.040.186984
CAACGCA65250.039.4384425
TATCAAC67250.038.311282
ATCAACG67100.038.308043
AACGCAG68300.037.870016
GTACATG96900.034.845881
TACATGG100400.032.611732
ACGCAGA81000.031.8551677
ACATGGG101250.031.631113
CGCAGAG84650.030.3365068
CATGGGG88100.030.3215084
ATGGGGG55500.029.1939245
GTGGTAT13750.029.0690521
GCAGAGT93800.027.3772439
TGGGGGG37900.025.7887926
AGAGTAC86100.024.63554210-11
CAGAGTA90150.024.28884710-11
TGGTATC16550.024.1441542
Produced by FastQC (version 0.11.5)