FastQCFastQC Report
Mon 15 Aug 2016
SRR2921643_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921643_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1547551
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40300.042.8312761
GGTATCA25500.040.3807141
AGCCTCG10600.037.613062
GTACATG94650.034.0208171
TCAACGC49650.033.918524
CAACGCA50400.033.5307665
TACATGG97550.033.1239662
TATCAAC51400.032.995252
CATGGGG81150.032.6318134
ATCAACG52400.032.1384473
AACGCAG53250.031.7351386
ACATGGG100550.031.3663143
ATGGGGG53550.030.7805215
ACGCAGA62050.027.1287
TATACCG1807.287308E-726.4468045
CGCAGAG67100.025.0863258
TGGGGGG39900.025.0540546
GTATAGG4750.023.808281
GCAGAGT73450.022.514089
GCCTCGG13850.022.3420643
Produced by FastQC (version 0.11.5)