FastQCFastQC Report
Mon 15 Aug 2016
SRR2921643_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921643_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1547551
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34180.22086509588375441No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26850.17349993635104755No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17970.11611895181483518No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA59500.047.209151
GGTATCA37450.044.270581
TATCAAC75400.037.3281632
TCAACGC76150.036.564254
CAACGCA76550.036.41575
ATCAACG79100.035.2794043
AACGCAG79500.035.2279476
GTACATG100800.031.3572221
TACATGG102000.030.6335332
ACGCAGA94800.029.5424257
CATGGGG80750.029.5343324
ACATGGG104100.029.4990733
ATGGGGG50900.028.9046125
CGCAGAG97900.028.484318
GAGTACT44050.026.69090512-13
GCAGAGT105950.026.2076159
GTACTTT46500.025.21326414-15
AGTACTT47400.024.30215312-13
TGGGGGG35800.023.9682376
AGAGTAC98300.022.30677610-11
Produced by FastQC (version 0.11.5)