FastQCFastQC Report
Mon 15 Aug 2016
SRR2921642_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921642_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1515076
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14350.054.3498541
GTATCAA24150.045.1141661
TCAACGC26750.039.605944
CAACGCA28150.037.845145
GTACATG71550.037.1943441
ATCAACG29500.036.317393
TACATGG74450.035.0965162
AACGCAG31000.034.165946
TATCAAC31950.033.34622
ACATGGG78550.033.340393
CATGGGG69650.031.6187554
TATACCG2001.8590072E-929.758125
ATGGGGG42850.028.7511355
GCCTAGT4800.028.5200824
ACGCAGA37250.027.9477217
ATGCCTA8100.026.4534112
CGCAGAG40050.025.8444358
GAGTACA51600.024.9220071
GTACCGT2651.378794E-924.699146
GTATAGG4100.024.6856211
Produced by FastQC (version 0.11.5)